Antimicrobial edible apple films inactivate antibiotic resistant and susceptible Campylobacter jejuni strains on chicken breast

Abstract: Campylobacter jejuni is the leading cause of bacterial diarrheal illness worldwide. Many strains are now becoming multidrug resistant. Apple‐based edible films containing carvacrol and cinnamaldehyde were evaluated for bactericidal activity against antibiotic resistant and susceptible C. jejuni strains on chicken. Retail chicken breast samples inoculated with D28a and H2a (resistant strains) and A24a (a sensitive strain) were wrapped in apple films containing cinnamaldehyde or carvacrol at 0.5%, 1.5%, and 3% concentrations, and then incubated at 4 or 23 °C for 72 h. Immediately after wrapping and at 72 h, samples were plated for enumeration of viable C. jejuni. The antimicrobial films exhibited dose‐ and temperature‐dependent bactericidal activity against all strains. Films with ≥1.5% cinnamaldehyde reduced populations of all strains to below detection at 23 °C at 72 h. At 4 °C with cinnamaldehyde, reductions were variable for all strains, ranging from 0.2 to 2.5 logs and 1.8 to 6.0 logs at 1.5% and 3.0%, respectively. Films with 3% carvacrol reduced populations of A24a and H2a to below detection, and D28a by 2.4 logs at 23 °C and 72 h. A 0.5‐log reduction was observed for both A24a and D28a, and 0.9 logs for H2a at 4 °C at 3% carvacrol. Reductions ranged from 1.1 to 1.9 logs and 0.4 to 1.2 logs with 1.5% and 0.5% carvacrol at 23 °C, respectively. The films with cinnamaldehyde were more effective than carvacrol films. Reductions at 23 °C were greater than those at 4 °C. Our results showed that antimicrobial apple films have the potential to reduce C. jejuni on chicken and therefore, the risk of campylobacteriosis. Possible mechanisms of antimicrobial effects are discussed. Practical Application: Apple antimicrobial films could potentially be used in retail food packaging to reduce C. jejuni commonly present on food.     

Analysis of Field Data on Computer Failures

This paper emphasizes the importance of making field measurements for effective and realistic dependability evaluatians.Two examples are given,both based on real data from IBM mainframes.The first evaluates the imnact of the operating environment on system failure characteristics and the second shows how an accurate model depicting this interaction can be extracted from real data.     

Designing an agent synthesis system for cross-RPC communication

Remote procedure call (RPC) is the most popular paradigm used today to build distributed systems and applications. As a consequence, the term “RPC” has grown to include a range of vastly different protocols above the transport layer. A resulting problem is that programs often use different RPC protocols, cannot be interconnected directly, and building a solution for each case in a large heterogeneous environment is prohibitively expensive. We describe the design of a system that can synthesize programs (RPC agents) to accommodate RPC heterogeneities. Because of its synthesis capability, the system also facilitates the design and implementation of new RPC protocols through rapid prototyping. We have built a prototype system to validate the design and to estimate the agent development costs and cross-RPC performance. The evaluation shows that the synthesis approach provides a more general solution than existing approaches do, and with lower software development and maintenance costs, while maintaining reasonable cross-RPC performance     

Genetic analysis of adult-onset cataract in a city-based ophthalmic hospital

When oxygen delivery (DO2) critically decreases, oxygen consumption (VO2) becomes supply dependent. We examined whether end-tidal PCO2 (PetCO2) would identify supply dependency during shock. Five dogs (Group I) underwent progressive hemorrhage to decrease DO2 until they could no longer maintain a stable blood pressure. Five additional animals (Group II) were bled until VO2 decreased to 70% of baseline, followed by resuscitation. The PetCO2 versus time inflection point was compared with the DO2 at onset of supply dependency (DO2crit). DO2crit for Groups I and II were 6.9 +/- .4 and 8.1 +/- 1.3, respectively (p = NS), and not statistically different from the DO2 values at which PetCO2 decreased (6.6 +/- .7 and 6.3 +/- .7 mL/kg per min, respectively). AT constant minute volume, PetCO2 effectively indicated the onset of supply dependency and rapidly increased during resuscitation, paralleling the changes in VO2 in this model of hemorrhagic shock.     

Voice transmission quality of mobile satellite communications systems

Recently, a number of commercial digital mobile satellite communications systems have become operational, and the introduction of more systems is imminent. The voice transmission quality of the Inmarsat-aeronautical, Inmarsat-M and Inmarsat-B systems was evaluated and compared with the quality of the Inmarsat-A analogue communication system on the basis of subjective listener opinion assessments. From this evaluation it can be concluded that, under nominal operating conditions, the current generation of digital mobile systems may offer a perceived performance that surpasses that of the older Inmarsat-A analogue system, at least when measured in terms of transmission quality.     

Effect of O2 scavenger on the shelf‐life of catfish (Pangasius sutchi) steaks during chilled storage

BACKGROUND: Active packaging for preserving meat products, including seafoods, has been gaining importance in recent times. The present study was undertaken to assess the effectiveness of O₂ scavenger, the most widely used active packaging technology, on the shelf‐life of catfish steaks in chilled storage conditions. RESULTS: O₂ scavenger was effective in reducing the oxygen concentration inside the packaging to as low as 0.42% within 24 h. This reduction in oxygen in O₂ scavenger packages showed positive effects on quality parameters compared to control air packs. Total volatile base nitrogen (TVB‐N), thiobarbituric acid value and peroxide value of catfish steaks packed in O₂ scavenger was significantly different (P < 0.05) compared to air‐packed samples. Based on the sensory, microbiological and TVB‐N values, the control samples were acceptable only up to 10 days, compared to 20 days in O₂ scavenger‐packed samples, extending the shelf‐life by 10 days. CONCLUSION: O₂ scavenger reduced the oxygen concentration inside the package significantly within 24 h compared to control air packs. By using this technique, the use of a vacuum packing machine can be avoided. Further, O₂ scavenger extended the product's shelf‐life up to 20 days, compared to 10 days in control packs. Copyright © 2007 Society of Chemical Industry     

Thermal and hydraulic characteristics of a triangular duct using an Al2O 3 nanofluid in a turbulent flow regime

This paper presents the computational analysis of convective heat transfer characteristics, pressure drop, and entropy generation characteristics of Al₂O₃/water nanofluids in a noncircular duct (triangular) using a single phase approach under a turbulent flow regime. The thermal and pressure drop characteristics of different concentrations of Al₂O₃ nanoparticles (NPs) and the analysis were carried out in Fluent software using a k‐ε approach under constant wall heat flux around the boundary. The results show that there is an increase in pressure drop and thereby an increase in friction by 20% for the smooth condition. The total pressure drop between the entry and exit section of the duct is increased to approximately 84.2% and 85.6% for a higher Reynolds number (Re = 10 000) compared with that of base fluid. Similarly, the entropy generation of water is increased by 40% as compared with 0.05% and 0.1% Al₂O₃ NPs. There is also a decrease in entropy generation identified while there is an increase in the Reynolds number. The convective heat transfer of 0.05% and 0.1% nanofluid has a similar trend with increased Reynolds number. The maximum performance is observed at the Reynolds number (Re = 4000) and found to be 1.29 for 0.1% concentration, whereas, the fluid at 0.05% is observed to be at 1.23. At a higher Reynolds number (Re = 10 000) the performance index decreased to approximately 1.19 and 1.25 for 0.05% and 0.1%, respectively.