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121.
A new method for seed oil body purification and examination of oil body integrity following germination 总被引:1,自引:0,他引:1
Plant seeds store triacylglycerols as energy sources for germination and postgerminative growth of seedlings. The triacylglycerols are preserved in small, discrete, intracellular organelles called oil bodies. A new method was developed to purify seed oil bodies. The method included extraction, flotation by centrifugation, detergent washing, ionic elution, treatment with a chaotropic agent, and integrity testing by use of hexane. These processes subsequently removed non-specifically associated or trapped proteins within the oil bodies. Oil bodies purified by this method maintained their integrity and displayed electrostatic repulsion and steric hindrance on their surface. Compared with the previous procedure, this method allowed higher purification of oil bodies, as demonstrated by SDS-PAGE using five species of oilseeds. Oil bodies purified from sesame were further analyzed by two-dimensional gel electrophoresis and revealed two potential oleosin isoforms. The integrity of oil bodies in germinating sesame seedlings was examined by hexane extraction. Our results indicated that consumption of triacylglycerols reduced gradually the total amount of oil bodies in seedlings, whereas no alteration was observed in the integrity of remaining oil bodies. This observation implies that oil bodies in germinating seeds are not degraded simultaneously. It is suggested that glyoxisomes, with the assistance of mitochondria, fuse and digest oil bodies one at a time, while the remaining oil bodies are preserved intact during the whole period of germination. 相似文献
122.
机械合金化过程中Fe70B30粉末晶粒尺寸和微观应变的研究 总被引:2,自引:0,他引:2
用X射线和电镜研究了Fe_(70)B_(30)粉末经不同时间高能球磨后晶粒尺寸和微观应力的变化。在机械合金化过程中,粉末的X射线衍射谱的宽度随球磨时间的增加逐渐加宽,这是晶粒细化和内部微观应力共同作用的结果。X射线衍射结果表明:随着机械合金化的进行,粉末的晶粒尺寸逐渐减小,球磨初期晶粒尺寸下降较快,经15h球磨,晶粒尺寸为25nm,机械合金化进行到一定时间后晶粒尺寸下降缓慢,80h球磨后晶粒尺寸可达5nm。在机械合金化过程中球磨所造成的微观应变不大,球磨初期粉末的内应力随球磨时间增加而增加,当粉末粒子尺寸很小时,随球磨的进行粉末中的微观应变显著下降。 相似文献
123.
Ku T.K. Chen S.H. Yang C.D. She N.J. Wang C.C. Chen C.F. Hsieh I.J. Cheng H.C. 《Electron Device Letters, IEEE》1996,17(5):208-210
Undoped and phosphorus (P)-doped diamond-clad Si field emitter arrays have been successfully fabricated using microwave plasma chemical vapor deposition (MPCVD) technology. The electron emission from the blunt diamond-clad microtips are much higher than those for the pure Si tips with sharp curvature due to a lower work function. Furthermore, the characteristics of emission current against applied voltage for the P-doped diamond-clad tips show superior emission at lower field to the undoped ones. After the examination of Auger electron spectroscopy (AES) and electrical characteristics of as-grown diamond, such a significant enhancement of the electron emission from the P-doped diamond-clad tips is attributed to a higher electron conductivity and defect densities 相似文献
124.
Wen-Chau Liu Lih-Wen Laih Shiou-Ying Cheng Wen-Lung Chang Wei-Chou Wang Jing-Yuh Chen Po-Hung Lin 《Electron Devices, IEEE Transactions on》1998,45(2):373-379
In this paper, a new multiple negative-differential-resistance (MNDR) device based on a metal-insulator-semiconductor-insulator-metal (MISIM)-like structure with step-compositional InxGa1-x As quantum wells has been fabricated and demonstrated. The interesting MNDR phenomena are found in the current-voltage (I-V) characteristics of this device. At room temperature, the triple switching behaviours and quadruple stable operation states are obtained. In addition, the sixfold switching behaviors and a staircase-shaped I-V characteristic are observed at -105°C. A sequential carrier accumulation at InGaAs subwells and the potential lowering process are used to qualitatively explain the interesting MNDR phenomena. From the experimental results, it is shown that the studied device has good potential in multiple-valued logic applications 相似文献
125.
Because causal relations are neither observable nor deducible, they must be induced from observable events. The 2 dominant approaches to the psychology of causal induction—the covariation approach and the causal power approach—are each crippled by fundamental problems. This article proposes an integration of these approaches that overcomes these problems. The proposal is that reasoners innately treat the relation between covariation (a function defined in terms of observable events) and causal power (an unobservable entity) as that between scientists' law or model and their theory explaining the model. This solution is formalized in the power PC theory, a causal power theory of the probabilistic contrast model (P. W. Cheng & L. R. Novick, 1990). The article reviews diverse old and new empirical tests discriminating this theory from previous models, none of which is justified by a theory. The results uniquely support the power PC theory. (PsycINFO Database Record (c) 2011 APA, all rights reserved) 相似文献
126.
乘性和加性噪声中谐波恢复的循环统计量方法 总被引:6,自引:0,他引:6
本文从循环平衡的观点出发来研究乘性和加性噪声中的谐波恢复问题.首先,在一定条件下建立了一般复过程的有限长付里叶变换的大样本性质.然后,得到了任意阶循环矩的样本估计关于循环频率的一致收敛速度.对于乘性和加性噪声中的谐波信号,建立了一、二、三阶循环矩样本估计的统计性质.在此基础上,分别提出了基于不同阶循环矩的谐波分最个数和频率的估计方法,并得到了估计的强相容性质和强收敛速度,最后给出了模拟实验结果. 相似文献
127.
128.
Restless legs syndrome affects millions, but most healthcare providers don't know it exists. Its sleep-deprived victims are plagued by indescribable crawling sensations in their legs that occur at night and that are only relieved by moving the legs. Many home healthcare clients, already challenged by their primary illness, are also affected by the persistent symptoms of Restless Legs Syndrome. It is important that home care nurses learn about Restless Legs Syndrome-what it is, how to assess it, and what can be done about it. 相似文献
129.