The Italian Wall Lizard Podarcis siculus as a Biological Model for Research in Male Reproductive Toxicology

Spermatogenesis is a genetically driven differentiation process that occurs in the testis and leads to the formation of spermatozoa. This process is extensively studied in several experimental models, particularly in vertebrates that share the morphological structure and functionality of the mammalian testis. Although reptiles are not generally considered biological models, the lizard Podarcis siculus has represented a suitable organism for the study of spermatogenesis over the years. In this lizard, the process of spermatogenesis is regulated by the interaction between systemic factors such as gonadotropins and local factors, i.e., molecules produced by the somatic and germinal cells of the testis. Many exogenous substances are able to alter the production of these regulative factors, thus altering the course of spermatogenesis, and P. siculus has proven to be an excellent model for studying the effects of various endogenous or exogenous substances on mechanisms underlying spermatogenesis. This review summarizes the available data on the effects of different substances on the control of spermatogenesis, highlighting the induced morphological and molecular alterations. Overall, the data show that sex hormone levels as well as the final stages of spermatogenesis are most affected by an imbalance of endogenous compounds or contamination by environmental pollutants. This is helpful for the male individual, since the damage, not affecting the spermatogonial stem cells, can be considered transient and not irreversible.     

Differential CFTR-Interactome Proximity Labeling Procedures Identify Enrichment in Multiple SLC Transporters

Proteins interacting with CFTR and its mutants have been intensively studied using different experimental approaches. These studies provided information on the cellular processes leading to proper protein folding, routing to the plasma membrane, recycling, activation and degradation. Recently, new approaches have been developed based on the proximity labeling of protein partners or proteins in close vicinity and their subsequent identification by mass spectrometry. In this study, we evaluated TurboID- and APEX2-based proximity labeling of WT CFTR and compared the obtained data to those reported in databases. The CFTR-WT interactome was then compared to that of two CFTR (G551D and W1282X) mutants and the structurally unrelated potassium channel KCNK3. The two proximity labeling approaches identified both known and additional CFTR protein partners, including multiple SLC transporters. Proximity labeling approaches provided a more comprehensive picture of the CFTR interactome and improved our knowledge of the CFTR environment.     

Chemical substances present in discharge water generated by laundry industry: Analytical monitoring

To our knowledge, precise data concerning the pollution in terms of qualitative and quantitative fluctuations in discharge water from the laundry sector have seldom been reported. This study investigated the chemical composition of the discharge water from a laundry industry. Over 160 chemical substances and 15 standard water parameters were monitored. The results showed that the discharge water presented both inorganic and organic polycontamination with a high degree of qualitative and quantitative variability. However, of all monitored substances, only five metals (Al, Cu, Fe, Sr, and Zn), five minerals (P, Ca, K, Na, and S), and alkylphenols were systematically present and quantifiable. For a daily average water flow of 129 m³, the released metal flux was 356 g/d. Substances, such as trichloromethane, brominated diphenyl ether (BDE) 47, and fluorides, were occasionally found and quantified. Other substances, such as chlorophenols, organo-tins, and pesticides were never identified. All the samples had quantifiable levels in the chemical oxygen demand (COD), biological oxygen demand (BOD), and hydrocarbons. Only the concentrations of Zn (8.3 g/d), Cu (21.4 g/d), and BOD (57.4 g/d) were close to or above the regulatory values: 74.0 g/d for Zn, 9.0 g/d for Cu, and 57.0 kg/d for BOD. The data obtained from this study are useful to the choice of additional treatments for the reduction of pollutant fluxes.     

Emerging Roles of the Iron Chelators in Inflammation

Iron is a crucial element for mammalian cells, considering its intervention in several physiologic processes. Its homeostasis is finely regulated, and its alteration could be responsible for the onset of several disorders. Iron is closely related to inflammation; indeed, during inflammation high levels of interleukin-6 cause an increased production of hepcidin which induces a degradation of ferroportin. Ferroportin degradation leads to decreased iron efflux that culminates in elevated intracellular iron concentration and consequently iron toxicity in cells and tissues. Therefore, iron chelation could be considered a novel and useful therapeutic strategy in order to counteract the inflammation in several autoimmune and inflammatory diseases. Several iron chelators are already known to have anti-inflammatory effects, among them deferiprone, deferoxamine, deferasirox, and Dp44mT are noteworthy. Recently, eltrombopag has been reported to have an important role in reducing inflammation, acting both directly by chelating iron, and indirectly by modulating iron efflux. This review offers an overview of the possible novel biological effects of the iron chelators in inflammation, suggesting them as novel anti-inflammatory molecules.     

Hydrogen evolution reaction in PTFE bonded Raney-Ni electrodes

This study is concerned with the hydrogen evolution reaction (HER) in several PTFE bonded Raney-Ni electrodes as function of temperature and treatments. The Mo-doped Raney-Ni catalysts are activated by hours of long cathodic polarization interleaved with few deep “charge - discharge” (polarity reversal) cycles. Moreover, the HER efficiency of the electrode requires additives which enhance conductivity and surface properties: with powders of Ni alloys (Ni-Ti, Ni-Cr, Ni-Fe) the electrode becomes also more stable, and almost insensitive to polarity reversal. The main effect of a temperature increase is the reduction of the Tafel slope, which is about 120 mV/dec at 25 °C, and about 60 mV/dec at 60 °C. A proper choice of additives yield electrodes which withstand polarity reversal and may be used in electrolysers which are intermittently operated, or have anodes which require periodic in situ re-activation by reduction.     

Efficient estimation of tuned liquid column damper inerter (TLCDI) parameters for seismic control of base-isolated structures

This paper presents an enhanced base-isolation (BI) system equipped with a novel passive control device composed of a tuned liquid damper and an inerter (TLCDI). With the aim of reducing the seismic response of BI systems, this contribution focuses on the design of the TLCDI providing analytical solutions for the optimal TLCDI parameters, easily implementable in the design phase. The effectiveness of the proposed approach in terms of seismic response reduction and computational gain is validated by comparison with classical numerical optimization techniques. The control performance of two different base-isolated TLCDI-controlled structures is assessed by employing real-ground motion records, and relevant comparisons with both uncontrolled base-isolated structures and equipped with a conventional TLCD are presented.     

The Facets of Diversity: The EFMC Perspective**

Diversity in science refers to cultivating talent, while promoting full inclusion across the community. In medicinal chemistry and chemical biology, it enhances creativity and encourages contributions from multiple perspectives, leading to better decision making and broader scientific impact. The European Federation for Medicinal chemistry and Chemical biology (EFMC) embraces and promotes diversity, to ensure representation of all talents, and enable equality of opportunity through fairness and transparency. EFMC has historically paid continuous attention to diversity in terms of culture, geography and equilibrium between academia and industry, with over the last few years a focus on increasing gender balance, aiming at a fair representation of the scientific community and equal opportunities independently of gender. EFMC promotes cultural diversity as it reinforces openness and mutual respect. All scientific organizations of a scope compatible with its remit are welcome within EFMC, where their members benefit from a welcoming, psychologically safe, and stimulating environment. Herein, we describe the state of diversity within the EFMC, how the situation has evolved over the years and where diversity should be further encouraged.     

Molecular Pathogenesis and Treatment Perspectives for Hypereosinophilia and Hypereosinophilic Syndromes

Hypereosinophilia (HE) is a heterogeneous condition with a persistent elevated eosinophil count of >350/mm³, which is reported in various (inflammatory, allergic, infectious, or neoplastic) diseases with distinct pathophysiological pathways. HE may be associated with tissue or organ damage and, in this case, the disorder is classified as hypereosinophilic syndrome (HES). Different studies have allowed for the discovery of two major pathogenetic variants known as myeloid or lymphocytic HES. With the advent of molecular genetic analyses, such as T-cell receptor gene rearrangement assays and Next Generation Sequencing, it is possible to better characterize these syndromes and establish which patients will benefit from pharmacological targeted therapy. In this review, we highlight the molecular alterations that are involved in the pathogenesis of eosinophil disorders and revise possible therapeutic approaches, either implemented in clinical practice or currently under investigation in clinical trials.     

PTX3 Effects on Osteogenic Differentiation in Osteoporosis: An In Vitro Study

Pentraxin 3 (PTX3) is a glycoprotein belonging to the humoral arm of innate immunity that participates in the body’s defence mechanisms against infectious diseases. It has recently been defined as a multifunctional protein, given its involvement in numerous physiological and pathological processes, as well as in the pathogenesis of age-related diseases such as osteoporosis. Based on this evidence, the aim of our study was to investigate the possible role of PTX3 in both the osteoblastic differentiation and calcification process: to this end, primary osteoblast cultures from control and osteoporotic patients were incubated with human recombinant PTX3 (hrPTX3) for 72 h. Standard osteinduction treatment, consisting of β-glycerophosphate, dexamethasone and ascorbic acid, was used as control. Our results showed that treatment with hrPTX3, as well as with the osteogenic cocktail, induced cell differentiation towards the osteoblastic lineage. We also observed that the treatment not only promoted an increase in cell proliferation, but also the formation of calcification-like structures, especially in primary cultures from osteoporotic patients. In conclusion, the results reported here suggest the involvement of PTX3 in osteogenic differentiation, highlighting its osteoinductive capacity, like the standard osteoinduction treatment. Therefore, this study opens new and exciting perspectives about the possible role of PTX3 as biomarker and therapeutic agent for osteoporosis.