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41.
A practical Raman analyzing technique with suppression of the strong fluorescent background in order to obtain quantitative information is proposed in the present study. The technique is based on the shifted excitation method and partial least squares regression (PLSR) analysis. The Raman system consists of a single Raman spectrometer, a background-free electrically tunable Ti:Sapphire laser (BF-ETL), and a micro-Raman probe (MRP). The system allows one to obtain reliable shifted excitation Raman spectra with a simple operation. The PLSR analysis successfully provides quantitative information from the obtained spectra with the suppression of random noise including photon shot noise. The present study demonstrates that the technique is effective for extracting quantitative information concealed behind a fluorescent background that is more than 200 times stronger than the Raman signal. 相似文献
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Mayuko Ikarashi Junko Tsuchida Masayuki Nagahashi Shiho Takeuchi Kazuki Moro Chie Toshikawa Shun Abe Hiroshi Ichikawa Yoshifumi Shimada Jun Sakata Yu Koyama Nobuaki Sato Nitai C. Hait Yiwei Ling Shujiro Okuda Kazuaki Takabe Toshifumi Wakai 《International journal of molecular sciences》2021,22(24)
Although numerous experiments revealed an essential role of a lipid mediator, sphingosine-1-phosphate (S1P), in breast cancer (BC) progression, the clinical significance of S1P remains unclear due to the difficulty of measuring lipids in patients. The aim of this study was to determine the plasma concentration of S1P in estrogen receptor (ER)-positive BC patients, as well as to investigate its clinical significance. We further explored the possibility of a treatment strategy targeting S1P in ER-positive BC patients by examining the effect of FTY720, a functional antagonist of S1P receptors, on hormone therapy-resistant cells. Plasma S1P levels were significantly higher in patients negative for progesterone receptor (PgR) expression than in those positive for expression (p = 0.003). Plasma S1P levels were also significantly higher in patients with larger tumor size (p = 0.012), lymph node metastasis (p = 0.014), and advanced cancer stage (p = 0.003), suggesting that higher levels of plasma S1P are associated with cancer progression. FTY720 suppressed the viability of not only wildtype MCF-7 cells, but also hormone therapy-resistant MCF-7 cells. Targeting S1P signaling in ER-positive BC appears to be a possible new treatment strategy, even for hormone therapy-resistant patients. 相似文献
44.
The effect of the signal transition variation, due to the signal transition density, data asymmetry, and sinusoidal modulation, on the performance of the data transition tracking loop (DTTL) symbol synchronizer is addressed. These factors will affect the DTTL performance, such as the clock jitter and the cycle slippage rate, and unnecessary system loss may result if the system parameters are not well selected. Given the background of C. S. Tsang and W. C. Lindsey (1986), this result can serve as an engineering design guideline for the DTTL in the context of the signal transition variation 相似文献
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Chie Ando Koichiro Tsuzuku Tomomi Kobayashi Hiroshi Kishi Shinichi Kuroda Mamoru Senna 《Journal of Materials Science: Materials in Electronics》2009,20(9):844-850
Effects of glycine added to BaCO3 and TiO2 mixtures were examined during a solid-state reaction toward phase pure BaTiO3 for MLCC with the average particle size below 180 nm and the tetragonality, c/a, above 1.009. Changes in the states of glycine
during heating were monitored by differential thermogravimetry and high-temperature X-ray photoelectron spectroscopy. Glycine
residue persists up to around 350 °C and destabilizes Ti–O bonds in TiO2 prior to the initiation of reaction toward BaTiO3. This, in turn, leads to a decrease in the reaction termination temperature due to higher mobility of Ba2+ ions through the reaction zone. 相似文献
47.
A general "phase-plane" technique for investigating the stability of nonlinear difference equation is described and applied to determine the stability criterion of a first-order digital phase-locked loop (DPLL). If the loop is stable, it is shown that acquisition behavior can be modeled as a first passage time problem. Using this model, one can evaluate the acquisition probability and the mean time to acquire, from the appropriate Chapman-Kolmogorov (C-K) equation describing the transition of the phase error sequence. This approach is verified by direct simulation. 相似文献
48.
Masaru Sato Hiroaki Tanaka Koji Inaka Shinichi Shinozaki Ari Yamanaka Sachiko Takahashi Mari Yamanaka Erika Hirota Shigeru Sugiyama Mitsuyasu Kato Chie Saito Satoshi Sano Moritoshi Motohara Tai Nakamura Tomoyuki Kobayashi Susumu Yoshitomi Tetsuo Tanaka 《Microgravity science and technology》2006,18(3-4):184-189
Since 2003, Japan Aerospace Exploration Agency (JAXA, former NASDA) has been conducting a project on a semi-annual basis (JAXA-GCF) to obtain high-quality protein crystals in the microgravity environment using the Russian transportation system. For this project, protein samples were mostly provided by Japanese users for whom JAXA provided technical and clerical support for crystallization experiments in microgravity. For the project, JAXA has constructed a user-friendly support service for microgravity experiments and provided regular and frequent flight opportunities. To simplify and improve technological matters, JAXA devised a gel-tube method crystallization device, which is effective both in space and on ground, based on the counter-diffusion technique. JAXA also provided ground-based techniques for efficient preliminary optimization of crystallization conditions using a 1-dimensional simulation and for harvesting and cryoprotecting crystals before X-ray diffraction experiments. These improvements have significantly increased the success rate of obtaining useful results. In conclusion, JAXA has developed technologies for growing, in microgravity, high-quality protein crystals, which may diffract up to atomic resolution, for a better understanding of 3-dimensional protein structures through X-ray diffraction experiments. 相似文献
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Notch effect in austenitic stainless steel under cyclic torsion is quite different depending on the superposition of static tension. In pure torsion, the rubbing of the serrated factory-roof type crack faces delays the crack growth along the notch root. Thus, the lifetime in notched specimen becomes longer than in smooth specimen. However, in cyclic torsion with static tension, the flat crack path and mean tensile stress reduce the influence of the crack face contact. Accordingly, shorter lifetime resulted from higher strain concentration at the notch root. Crack growth in low carbon steel under cyclic torsion is highly affected by the ferrite/pearlite banded microstructure besides the addition of static tension. Because of a small amount of the crack face contact, the reduction of lifetime in notched specimen is revealed irrespective of superposition of static tension. 相似文献