全文获取类型
收费全文 | 14547篇 |
免费 | 760篇 |
国内免费 | 41篇 |
专业分类
电工技术 | 171篇 |
综合类 | 16篇 |
化学工业 | 2681篇 |
金属工艺 | 449篇 |
机械仪表 | 939篇 |
建筑科学 | 230篇 |
矿业工程 | 3篇 |
能源动力 | 438篇 |
轻工业 | 1102篇 |
水利工程 | 83篇 |
石油天然气 | 13篇 |
无线电 | 2351篇 |
一般工业技术 | 2758篇 |
冶金工业 | 2503篇 |
原子能技术 | 167篇 |
自动化技术 | 1444篇 |
出版年
2024年 | 13篇 |
2023年 | 164篇 |
2022年 | 231篇 |
2021年 | 437篇 |
2020年 | 283篇 |
2019年 | 326篇 |
2018年 | 367篇 |
2017年 | 420篇 |
2016年 | 457篇 |
2015年 | 379篇 |
2014年 | 554篇 |
2013年 | 825篇 |
2012年 | 833篇 |
2011年 | 1015篇 |
2010年 | 735篇 |
2009年 | 782篇 |
2008年 | 722篇 |
2007年 | 569篇 |
2006年 | 494篇 |
2005年 | 447篇 |
2004年 | 413篇 |
2003年 | 369篇 |
2002年 | 383篇 |
2001年 | 292篇 |
2000年 | 262篇 |
1999年 | 300篇 |
1998年 | 894篇 |
1997年 | 527篇 |
1996年 | 398篇 |
1995年 | 231篇 |
1994年 | 199篇 |
1993年 | 210篇 |
1992年 | 84篇 |
1991年 | 79篇 |
1990年 | 67篇 |
1989年 | 92篇 |
1988年 | 51篇 |
1987年 | 63篇 |
1986年 | 34篇 |
1985年 | 48篇 |
1984年 | 16篇 |
1983年 | 9篇 |
1982年 | 21篇 |
1981年 | 23篇 |
1980年 | 18篇 |
1978年 | 8篇 |
1977年 | 62篇 |
1976年 | 124篇 |
1975年 | 4篇 |
1973年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 656 毫秒
101.
102.
103.
104.
The semicontinuous separation/concentration of two solutes with different molecular size by the size exclusion cyclic separation
method is based on the opposite swelling responses of two gels to a temperature change. Experimental results of separation
and concentration of solutes are verified qualitatively by the theoretical models based on the local equilibrium assumption.
Separation of two solutes is shown by the breakthrough curves in coupled gel columns. In closed coupled columns, the volumetric
space for the large molecules which are totally excluded from the gels becomes smaller, creating a concentrating effect as
the gels swell when temperature changes. A mechanistic model is suggested to predict the large molecule concentration to increase
to its solubility limit as cycle repeats. 相似文献
105.
106.
Dream work in therapeutic environments is reviewed, exploring the benefits and limitations of dreams. The application of dreams to groups and the impact of the work on group process and interaction is discussed. The integration of dream-work models within various group psychotherapeutic approaches is examined. A meta-classification of dream-work concludes the review. 相似文献
107.
108.
DG Robertson TK Braden ER Urda ND Lalwani FA de la Iglesia 《Canadian Metallurgical Quarterly》1998,72(6):362-371
Tetrahydroaminoacridine (tacrine) causes morphological and functional changes in the endoplasmic reticulum, ribosomes, and mitochondria in the liver of humans and animals. In order to investigate species differences as well as to understand the morphological changes, we examined the effects of tacrine on respiration and electron transport in mitochondria isolated from rat, dog, monkey, and human liver. Tacrine produced significantly decreased respiratory control ratios (RCR) in all species at concentrations ranging from 5 to 25 microg/ml. Human mitochondria were more sensitive to tacrine effects with RCR decreased 24% at 5 microg/ml while other species were unaffected at this concentration. The tacrine effects were characterized by increased hepatic mitochondrial State 4 respiration in rats and decreased State 3 respiration in humans. Mitochondria from aged rats were more sensitive to the effects of tacrine than mitochondria from young animals, with significantly decreased RCR at 10 microg/ml in aged rats while mitochondria from young rats were unaffected at this concentration. Concomitant with the respiratory changes, mitochondrial DNA synthesis was impaired. Since tacrine undergoes extensive biotransformation, we also explored the possibility that metabolites could exert detrimental effects. The ranking order of potency for decreasing RCR caused by monohydroxylated metabolites was: tacrine > 4-OH and 7-OH > 2-OH, 1-OH, and velnacrine with the latter group of metabolites having no effect on mitochondrial respiration at concentrations up to 50 microg/ml. In vivo administration of 20 mg/kg tacrine to rats for up to 20 days caused a paradoxical increase in RCR and P/O on Day 1 and decreased RCR on Days 9 and 20, the later findings being consistent with in vitro data. From these data we propose that tacrine does not necessarily have to be metabolized to exert effects on mitochondria at different sites in the electron transport chain that differ among species. These effects are exacerbated in mitochondria from older animals and humans appear to be more sensitive than the laboratory animals studied. 相似文献
109.
Copper amine oxidase contains a covalently bound quinonoid cofactor, 2,4,5-trihydroxyphenylalanyl quinone (TPQ), which is synthesized by post-translational modification of a specific tyrosyl residue occurring in the highly conserved sequence, Asn-Tyr-(Asp/Glu)-Tyr. To elucidate the role(s) of the conserved sequence in the biogenesis of TPQ, each of the corresponding residues at positions 401-404 in the recombinant histamine oxidase from Arthrobacter globiformis has been replaced with other amino acids by site-directed mutagenesis. When Asn-401 was changed to Asp or Gln, the rate of TPQ formation by copper-dependent self-processing was 10(3)- to 10(4)-fold slower than in the wild-type enzyme. When Tyr-402 was replaced by Phe, TPQ was not formed at all, showing that Tyr-402 is essential as the precursor to TPQ. In contrast, Asp-403 could be replaced by Glu without changes in the rate of TPQ formation, whereas its replacement by Asn led to a marked decrease. Furthermore, when Tyr-404 was changed to Phe, TPQ was formed swiftly on incubation with copper ions, but the TPQ enzyme exhibited very low activity with altered substrate specificity. These results collectively indicate that a very rigorous structural motif is required for efficient formation of TPQ and for the catalytic activity in the active site of copper amine oxidases. 相似文献
110.
It is known that the phosphorylation of two serine residues on the NH2-terminal extension specific to cardiac troponin-I (Tn-I) modulates the calcium-dependent activation of the myofilaments. The process by which this occurs remains an unsolved puzzle. We have applied a dissective approach to study the effect of this phosphorylation on the interactions between Tn-I and its partner proteins, actin and troponin-C (Tn-C). Using N-[14C]ethylmaleimide-labelled Tn-I in sedimentation assays with F-actin, we found that the dephosphorylated Tn-I binds to F-actin with pronounced positive cooperativity, both in the absence and the presence of tropomyosin. Phosphorylation of the protein slightly weakens the interaction in the absence of tropomyosin, but the cooperativity remained. In the presence of tropomyosin, phosphorylation of the Tn-I also appeared to slightly weaken the interaction as well but, more significantly, the cooperativity was eliminated. These data can only be explained simply by a cooperative interaction between the monomer units in the actin filament. The interactions between cardiac Tn-I and Tn-C were studied by labelling the Tn-C with the fluorescent probe dansyl aziridine. As expected, the binding of the dephosphorylated Tn-I to Tn-C was strengthened by over 20-fold upon the addition of calcium to the assay. Phosphorylation of the protein, however, had a dramatic effect on the interaction in that it appeared to desensitise the complex to the effect of calcium: the Ka values obtained for both interactions (+/- Ca2+) were almost identical. These results clearly indicate that the phosphorylation of Tn-I in the cardiac system has dramatic effects on the isolated inter-protein interactions. We also discuss the possible significance of such an effect on the interactions of the isolated proteins in their roles within the intact cardiac regulatory complex. 相似文献