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131.
Christina Borch-Jensen Benny Jensen Kim Mathiasen Jørgen Mollerup 《Journal of the American Oil Chemists' Society》1997,74(3):277-284
The seed oils from Dimorphoteca pluvialis and Ricinus communis contain hydroxy fatty acids. Dimorphoteca pluvialis contains Δ-9-hydroxy-10t, 12t-octadecadienoic acid (dimorphecolic acid) and R. communis contains Δ-12-hydroxy-9c-octadecenoic acid (ricinoleic acid). The oils were derivatized and analyzed to determine the content of hydroxy fatty acids.
The trimethylsilyl fatty acid methyl ester (TMS-FAME) derivatives were analyzed by capillary gas chromatography (GC), and
the free fatty acid (FFA) derivatives and the oils were analyzed by capillary supercritical fluid chromatography (SFC). Further,
mass spectroscopy of the TMS-FAME derivatives was performed to check the purity of the derivatives. The results from the GC
analyses of TMS-FAME corresponded to the results found by SFC analysis of the FFA. The content of ricinoleic acid in the glycerolipids
of R. communis was 87.7 wt%, and the content of dimorphecolic acid in D. pluvialis was 54.0 wt%. The methods were evaluated with respect to the cost, ease, and time needed for sample preparation and analysis. 相似文献
132.
133.
Polymeric Micelle‐Mediated Delivery of DNA‐Targeting Organometallic Complexes for Resistant Ovarian Cancer Treatment 下载免费PDF全文
Xiaopin Duan Demin Liu Christina Chan Wenbin Lin 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(32):3962-3972
Three half‐sandwich iridium and ruthenium organometallic complexes with high cytotoxicity are synthesized, and their anticancer mechanisms are elucidated. The organometallic complexes can interact with DNA through coordination or intercalation, thereby inducing apoptosis and inhibiting proliferation of resistant cancer cells. The organometallic complexes are then incorporated into polymeric micelles through the polymer‐metal coordination between poly(ethylene glycol)‐b‐poly(glutamic acid) [PEG‐b‐P(Glu)] and organometallic complexes to further enhance their anticancer effects as a result of the enhanced permeability and retention effect. The micelles with particle sizes of ≈60 nm are more efficiently internalized by cancer cells than the corresponding complexes, and selectively dissociate and release organometallic anticancer agents within late endosomes and lysosomes, thereby enhancing drug delivery to the nuclei of cancer cells and facilitating their interactions with DNA. Thus, the micelles display higher antitumor activity than the organometallic complexes alone with a lack of the systemic toxicity in a mouse xenograft model of cisplatin‐resistant human ovarian cancer. These results suggest that the polymeric micelles carrying anticancer organometallic complexes provide a promising platform for the treatment of resistant ovarian cancer and other hard‐to‐treat solid tumors. 相似文献
134.
135.
Schwaiger K Helmke K Hölzel CS Bauer J 《International journal of food microbiology》2011,148(3):191-196
The aim of this study was to elucidate whether and to what extent fresh produce from Germany plays a role as a carrier and reservoir of antibiotic resistant bacteria. For this purpose, 1001 vegetables (fruit, root, bulbous vegetables, salads and cereals) were collected from 13 farms and 11 supermarkets in Germany and examined bacteriologically. Phenotypic resistance of Enterobacter cloacae (n = 172); Enterobacter gergoviae (n = 92); Pantoea agglomerans (n = 96); Pseudomonas aeruginosa (n = 295); Pseudomonas putida (n = 106) and Enterococcus faecalis (n = 100) against up to 30 antibiotics was determined by using the microdilution method. Resistance to ß-lactams was most frequently expressed by P. agglomerans and E. gergoviae against cefaclor (41% and 29%). Relatively high resistance rates were also observed for doxycycline (23%), erythromycin (21%) and rifampicin (65%) in E. faecalis, for spectinomycin (28%) and mezlocillin (12%) in E. cloacae, as well as for streptomycin (19%) in P. putida. In P. aeruginosa, relatively low resistance rates were observed for the aminoglycosides amikacin, apramicin, gentamicin, neomycin, netilmicin and tobramycin (< 4%); 11% was resistant to streptomycin. No glycopeptide-resistant enterococci were observed. Resistance rates of bacteria isolated from farm samples were higher than those of the retail markets whenever significant differences were observed. This suggests that expressing resistance is at the expense of bacterial viability, since vegetables purchased directly at the farm are probably fresher than at the supermarket, and they have not been exposed to stress factors. However, this should not keep the customer from buying directly at the farm, since the overall resistance rates were not higher than observed in bacteria from human or animal origin. Instead, peeling or washing vegetables before eating them raw is highly recommended, since it reduces not only the risk of contact with pathogens, but also that of ingesting and spreading antibiotic resistant bacteria. 相似文献
136.
Schofer MD Veltum A Theisen C Chen F Agarwal S Fuchs-Winkelmann S Paletta JR 《Journal of materials science. Materials in medicine》2011,22(7):1753-1762
Mesenchymal stem cell differentiation of osteoblasts is triggered by a series of signaling processes including integrin and
bone morphogenetic protein (BMP), which therefore act in a cooperative manner. The aim of this study was to analyze whether
these processes can be remodeled in an artificial poly-(l)-lactide acid (PLLA) based nanofiber scaffold. Matrices composed of PLLA-collagen type I or BMP-2 incorporated PLLA-collagen
type I were seeded with human mesenchymal stem cells (hMSC) and cultivated over a period of 22 days, either under growth or
osteoinductive conditions. During the course of culture, gene expression of alkaline phosphatase (ALP), osteocalcin (OC) and
collagen I (COL-I) as well as Smad5 and focal adhesion kinase (FAK), two signal transduction molecules involved in BMP-2 or
integrin signaling were analyzed. Furthermore, calcium and collagen I deposition, as well as cell densities and proliferation,
were determined using fluorescence microscopy. The incorporation of BMP-2 into PLLA-collagen type I nanofibers resulted in
a decrease in diameter as well as pore sizes of the scaffold. Mesenchymal stem cells showed better adherence and a reduced
proliferation on BMP-containing scaffolds. This was accompanied by an increase in gene expression of ALP, OC and COL-I. Furthermore
the presence of BMP-2 resulted in an upregulation of FAK, while collagen had an impact on the gene expression of Smad5. Therefore
these different strategies can be combined in order to enhance the osteoblast differentiation of hMSC on PLLA based nanofiber
scaffold. By doing this, different signal transduction pathways seem to be up regulated. 相似文献
137.
Kevin P. Musselman Andrew Marin Andreas Wisnet Christina Scheu Judith L. MacManus‐Driscoll Lukas Schmidt‐Mende 《Advanced functional materials》2011,21(3):573-582
A novel buffering method is presented to improve the stability of zinc oxide processed in aqueous solutions. By buffering the aqueous solution with a suitable quantity of sacrificial zinc species, the dissolution of functional zinc oxide structures and the formation of unwanted impurities can be prevented. The method is demonstrated for ZnO films and nanowires processed in aqueous solutions used for the selective etching of mesoporous anodic alumina templates and the electrochemical deposition of Cu2O. In both cases, improved ZnO stability is observed with the buffering method. ZnO‐Cu2O heterojunction solar cells (bilayer and nanowire cells) synthesized using both traditional and buffered deposition methods are characterized by impedance spectroscopy and solar simulation measurements. Buffering the Cu2O deposition solution is found to reduce unwanted recombination at the heterojunction and improve the photovoltaic performance. 相似文献
138.
Young CR Menegazzo N Riley AE Brons CH DiSanzo FP Givens JL Martin JL Disko MM Mizaikoff B 《Analytical chemistry》2011,83(16):6141-6147
Simultaneous and molecularly selective parts-per-billion detection of benzene, toluene, and xylenes (BTX) using a thermal desorption (TD)-FTIR hollow waveguide (HWG) trace gas sensor is demonstrated here for the first time combining laboratory calibration with real-world sample analysis in field. A calibration range of 100-1000 ppb analyte/N(2) was developed and applied for predicting the concentration of blinded environmental air samples within the same concentration range, and demonstrate close agreement with the validation method used here, GC-FID. The analyte concentration prediction capability of the TD-FTIR-HWG trace gas sensor also compares well with the industrial standard and other experimental techniques including GC-PID, ultrafast GC-FID, and GC-DMS, which were simultaneously operated in the field. With the advent of a quantum cascade laser with emission frequencies specifically tailored to efficiently overlap benzene absorption as the most relevant analyte, the overall sensor footprint could be considerably reduced to ultimately yield hand-held trace gas sensors facilitating direct and real-time detection of BTX in air down to low ppb levels. 相似文献
139.
Individual safety performance in the construction industry: development and validation of two short scales 总被引:1,自引:0,他引:1
DeArmond S Smith AE Wilson CL Chen PY Cigularov KP 《Accident; analysis and prevention》2011,(3):948-954
In the current research a short measure of safety performance is developed for use in the construction industry and the relationships between different components of safety performance and safety outcomes (e.g., occupational injuries and work-related pain) are explored within the construction context. This research consists of two field studies. In the first, comprehensive measures of safety compliance and safety participation were shortened and modified to be appropriate for use in construction. Evidence of reliability and validity is provided. Both safety compliance and safety participation were negatively related to occupational injuries, yet these two correlations were not statistically different. In the second study, we investigated the relationships between these two components of safety performance and work-related pain frequency, in addition to replicating Study 1. Safety compliance had a stronger negative relationship with pain than safety participation. Implications for research are discussed. 相似文献
140.