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991.
Combined use of confocal laser scanning microscopy (CLSM) and Raman microscopy (RM): Investigations on EPS - Matrix 总被引:2,自引:0,他引:2
Confocal laser scanning microscopy (CLSM) was applied in combination with Raman microscopy (RM) for the characterization of heterotrophic biofilms. Compared to CLSM, RM allows for a deeper insight into the chemical structure of extracellular polymeric substances (EPS) of the biofilm matrix. A low load of glucose (2 g m−2 d−1) was applied as substrate to ensure small growth rates of the heterotrophic biofilm. To investigate the influence of hydrodynamic conditions on the chemical composition of EPS, a three funnel flow system was used, wherein biofilms were grown at Reynolds numbers of 1000, 2500 and 4000, respectively.31 and 92 days after inoculation with activated sludge supernatant RM was applied as an additional technique to standard CLSM measurements for a more detailed analysis of the biofilm matrix. Polysaccharide-related Raman bands are in good agreement with the lectin binding analysis from CLSM. For the older biofilm, lectin binding analysis showed no change in the composition of EPS, whereas Raman spectra pointed out a change of EPS composition from predominantly polysaccharides to predominantly (glyco) proteins. For the applied substrate condition no significant influence of the Reynolds number on the chemical properties was observed. 相似文献
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Spundwände werden im Wesentlichen auf Biegung infolge einer Belastung durch Erd‐ und Wasserdrücke beansprucht. Sie können aber auch zum Abtrag von Vertikallasten dienen. Das Tragverhalten von Spundwänden ist bei genauer Betrachtung äußerst komplex, da diesem eine räumlich und zeitlich gekoppelte Boden‐Bauwerk‐Interaktion zugrunde liegt. Für die praktische Bemessung muss das Tragverhalten daher stark idealisiert werden. Die Rechtfertigung der dafür notwendigen Modellvorstellungen sowie deren Vereinfachungen und Annnahmen sind, wie häufig in der Geotechnik, hauptsächlich durch Erfahrung begründet. Researching the vertical load bearing behaviour of sheet piles. Sheet pile walls are mainly used to carry loads caused by earth and water pressure via bending. In special cases these walls can be used to transfer vertical loads to the subsoil. The complex load bearing behaviour of sheet pile walls is caused by the sterical and temporal coupled soil structure interaction. For practical design the load bearing behaviour needs to be idealised. The apologies used to explain the model conceptions as well as their simplifications are, as usual in geotechnics, mainly motivated by operating experience. 相似文献
995.
Jens Kappes Mathias Liewald Simon Jupp Christoph Pirchl Roman Herstelle 《Production Engineering》2012,6(3):219-228
Relatively low tooling costs, high design complexity coupled with low forming speeds make the superplastic sheet metal forming process attractive, especially for smaller lot sizes. Due to the relatively small lot size, the effort and budget for designing superplastic forming processes is usually limited (Kappes and Liewald in J Mater Sci Eng B1:472?C478, 2011). For this reason the tool design and corresponding pressure profiles in superplastic forming processes are often based on trial and error (Franchitti et al. in 11th international Esaform conference on material forming, 2008; Barnes in J Mater Eng Perform 4:440?C454, 2007). Consequently a process chain should be established to design superplastic forming processes accurately and efficiently. This paper deals with the process chain to form an aluminium part superplastically. At the beginning of the process chain, there is a new, developmental aluminium alloy sheet (AA5456, s0?=?1.6?mm) designed for superplastic forming supplied by Hydro Aluminium Rolled Products GmbH. The relevant material parameters of this sheet are then determined via pneumatic bulge testing with and without in situ measurement of strains. Using these experimentally determined parameters superplastic forming process can be simulated by FE modelling (PAM-STAMP 2G). Due to in situ measurement of strains during pneumatic bulging, the comparison of experiment and FE-simulation results over the whole pneumatic bulging process could be done. This comparison shows good correlation for the observed conditions. Furthermore a cylindrical cup was simulated, evaluated via determined isobar Superplastic Forming Limit Curve (at fracture) and finally formed by pneumatic bulging. Material characterisation of the bottom of this cup showed that excessive cavitation was observed as a result of the iron-silicon particles. Superplastic forming of a bracket usually formed out of AA5083 was also simulated using material parameters of AA5456. The simulation was able to show that this part is not able to be manufactured out of AA5456 under these forming conditions, which was confirmed by forming trials performed at ALU-SPF AG. 相似文献
996.
Brochhausen C Schmitt VH Rajab TK Planck CN Krämer B Tapprich C Wallwiener M Hierlemann H Planck H Kirkpatrick CJ 《Journal of materials science. Materials in medicine》2012,23(8):1931-1939
Separation of traumatized tissue represents the only promising strategy in postoperative adhesion prevention, a relevant clinical problem after surgical intervention. In the present study scanning electron microscopy (SEM) and subsequent morphometry were used to analyse the tissue response to five commercial adhesion barriers. Standardised peritoneal lesions in Wistar rats were covered with solid and viscous barrier materials and semiquantitatively analysed 14 days postoperatively. Striking morphological differences in lesion surface organisation between the barrier groups became apparent with colonisation of the barrier by mesothelial cells to different degrees. Furthermore, the mesothelial cells showed either a normal or activated phenotype depending on the underlying biomaterial. These experiments demonstrate that the examination by SEM gives useful insights into the performance of barrier materials and the cellular processes of adhesion prevention, since mesothelial cells play an active role in the pathogenesis of adhesion formation. 相似文献
997.
Lenzen C Wattenhofer R 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2012,370(1958):11-26
Distributed algorithms are an established tool for designing protocols for sensor networks. In this paper, we discuss the relation between distributed computing theory and sensor network applications. We also present a few basic and illustrative distributed algorithms. 相似文献
998.
Christoph Czaderski Enzo Martinelli Julien Michels Masoud Motavalli 《Composites Part B》2012,43(2):398-410
Civil structures such as bridges and buildings can be strengthened with prestressed fibre reinforced polymer (FRP) strips to enhance both their stiffness and load-bearing capacity. End anchorage is a crucial issue for prestressed FRP strips. An innovative anchorage procedure, called the “gradient anchorage method” and based on the possible accelerated curing of the epoxy-resin in the end region of the FRP strip, has recently been conceived with the aim of avoiding more invasive mechanical fastening systems. An in-depth knowledge of the actual development of the key mechanical properties of resins under different curing conditions (i.e., in terms of curing temperature) is of paramount importance for employing the above mentioned gradient method in practical applications. This paper presents experimental results and analytical investigations aimed at developing a better understanding of the strength development of a commercial adhesive under different curing times and temperatures. Firstly, direct tensile tests on epoxy specimens were performed at different curing temperatures. It was shown that the necessary curing time to reach the maximum tensile strength can be significantly reduced from several hours at room temperature to approximately 30 min at 90 °C. Furthermore, higher curing temperatures reduced the activation time after which strength starts to increase. The experimental observations are shown graphically with both the activation time and reaction duration at different curing temperatures. Secondly, pull-off bond tests were conducted on 100 mm wide and 1.2 mm thick FRP strips bonded to concrete using epoxy adhesives cured either at 90 °C for different durations or at room temperature. An optical image correlation system (ICS) allowed the load transfer behaviour of the inhomogeneous cured adhesive between the FRP strip(s) and concrete to be studied. Finally, using the experimental measurements, the bond shear stress–slip interface relationships for the different test specimens were identified in order to present the effect of elevated curing temperatures and curing durations. 相似文献
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1000.
Indirect nanoplasmonic sensing (INPS) is an experimental platform exploiting localized surface plasmon resonance (LSPR) detection of processes in nanomaterials, molecular assemblies, and films at the nanoscale. Here we have for the first time applied INPS to study dye molecule adsorption/impregnation of two types of TiO(2) materials: thick (10 μm) mesoporous films of the kind used as photoanode in dye-sensitized solar cells (DSCs), with particle/pore size in the range of 20 nm, and thin (12-70 nm), dense, and flat films. For the thick-film experiments plasmonic Au nanoparticles were placed at the hidden, internal interface between the sensor surface and the mesoporous TiO(2). This approach provides a unique opportunity to selectively follow dye adsorption locally in the hidden interface region inside the material and inspires a generic and new type of nanoplasmonic hidden interface spectroscopy. The specific DSC measurement revealed a time constant of thousands of seconds before the dye impregnation front (the diffusion front) reaches the hidden interface. In contrast, dye adsorption on the dense, thin TiO(2) films exhibited much faster, Langmuir-like monolayer formation kinetics with saturation on a time scale of order 100 s. This new type of INPS measurement provides a powerful tool to measure and optimize dye impregnation kinetics of DSCs and, from a more general point of view, offers a generic experimental platform to measure adsorption/desorption and diffusion phenomena in solid and mesoporous systems and at internal hidden interfaces. 相似文献