稀土和碱土在热镀合金中应用的研究

研究了钢件热浸镀Fe-Al-RE(Sr)的工艺,测定了镀层的性能,分析了镀层结构与性能之间的关系     

基于邻居决策的协同过滤推荐算法

协同过滤技术应用于个性化推荐系统中,稀疏性问题和可扩展性问题成为亟需解决的问题。针对传统方法的不足,提出一种凭借邻居数做决策的方法,比较各个待测位置的用户邻居数和项目邻居数,由数量多的一方作预测,同时对预测值判定给出一种合理而有效的度量方法。实验结果表明,该方法能够提高推荐质量。     

基于小波的继电保护装置电磁干扰软件防护快速算法研究

基于Mallat分解的小波变换方法能可靠地检测出电力波形中的奇异点,但不适合于实际工程应用。提出了基于S变换的改进的快速算法,即对AD采样点进行前向差分。对于正常的正弦采样信号,点与点之间的采样点值相差较小,所以差分值也较小;而对于奇异信号,在奇异点附近AD采样值有突变,所以差分值较大。因此,选择合适的差分门槛值就可以检测出信号中的奇异点。分析奇异点附近差分值的正负特性可以检测出该奇异点是正常的故障信号或是受干扰信号。最后,通过对典型波形的仿真计算和实际的电磁兼容性试验,验证了该软件抗干扰措施能显著提高数字式继电保护装置运行的稳定性。     

平行单级双倒立摆系统的建模与滑模变结构控制

从两摆间无弹簧连接的平行双倒立摆系统特定物理结构出发,结合控制目标进行合理建模与分析,验证所建模型可行性,讨论系统能控性和参数间的关系,并设计了滑模变结构控制律.该文的理论和方法对平行单级双倒立摆的进一步研究工作具有重要意义.     

基于调峰形势的联络线受电模型及其优化

探讨了基于调峰形势的联络线受电优化模型。该模型根据负荷预测数据,采用分时电价计算方法,综合考虑电网内机组的发电、联络线受电和调峰能力三者的关系,在保证电网安全的条件下,得到了经济效益优化的受电曲线,从而改变了以往受电曲线由人工凭经验制定的状况,为电网的安全经济运行提供了技术依据。     

Generalized Approach towards Secretion-Based Protein Production via Neutralization of Secretion-Preventing Cationic Substrate Residues

Many heterologous proteins can be secreted by bacterial ATP-binding cassette (ABC) transporters, provided that they are fused with the C-terminal signal sequence, but some proteins are not secretable even though they carry the right signal sequence. The invention of a method to secrete these non-secretable proteins would be valuable both for understanding the secretory physiology of ABC transporters and for industrial applications. Herein, we postulate that cationic “supercharged” regions within the target substrate protein block the secretion by ABC transporters. We also suggest that the secretion of such substrate proteins can be rescued by neutralizing those cationic supercharged regions via structure-preserving point mutageneses. Surface-protruding, non-structural cationic amino acids within the cationic supercharged regions were replaced by anionic or neutral hydrophilic amino acids, reducing the cationic charge density. The examples of rescued secretions we provide include the spike protein of SARS-CoV-2, glutathione-S-transferase, streptavidin, lipase, tyrosinase, cutinase, growth factors, etc. In summary, our study provides a method to predict the secretability and a tool to rescue the secretion by correcting the secretion-blocking regions, making a significant step in understanding the physiological properties of ABC transporter-dependent protein secretion and laying the foundation for the development of a secretion-based protein-producing platform.     

Nanoscale Calculation of Proton-Induced DNA Damage Using a Chromatin Geometry Model with Geant4-DNA

Monte Carlo simulations can quantify various types of DNA damage to evaluate the biological effects of ionizing radiation at the nanometer scale. This work presents a study simulating the DNA target response after proton irradiation. A chromatin fiber model and new physics constructors with the ELastic Scattering of Electrons and Positrons by neutral Atoms (ELSEPA) model were used to describe the DNA geometry and the physical stage of water radiolysis with the Geant4-DNA toolkit, respectively. Three key parameters (the energy threshold model for strand breaks, the physics model and the maximum distance to distinguish DSB clusters) of scoring DNA damage were studied to investigate the impact on the uncertainties of DNA damage. On the basis of comparison of our results with experimental data and published findings, we were able to accurately predict the yield of various types of DNA damage. Our results indicated that the difference in physics constructor can cause up to 56.4% in the DNA double-strand break (DSB) yields. The DSB yields were quite sensitive to the energy threshold for strand breaks (SB) and the maximum distance to classify the DSB clusters, which were even more than 100 times and four times than the default configurations, respectively.