全文获取类型
收费全文 | 293篇 |
免费 | 27篇 |
专业分类
综合类 | 1篇 |
化学工业 | 110篇 |
金属工艺 | 3篇 |
机械仪表 | 3篇 |
建筑科学 | 9篇 |
能源动力 | 8篇 |
轻工业 | 50篇 |
石油天然气 | 3篇 |
无线电 | 9篇 |
一般工业技术 | 66篇 |
冶金工业 | 19篇 |
自动化技术 | 39篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 30篇 |
2021年 | 36篇 |
2020年 | 13篇 |
2019年 | 9篇 |
2018年 | 8篇 |
2017年 | 12篇 |
2016年 | 11篇 |
2015年 | 15篇 |
2014年 | 15篇 |
2013年 | 14篇 |
2012年 | 16篇 |
2011年 | 20篇 |
2010年 | 12篇 |
2009年 | 15篇 |
2008年 | 10篇 |
2007年 | 11篇 |
2006年 | 16篇 |
2005年 | 8篇 |
2004年 | 6篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 7篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1988年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有320条查询结果,搜索用时 15 毫秒
171.
Michela Valentini Gianpiero Groppi Cinzia Cristiani Marinella Levi Enrico Tronconi Pio Forzatti 《Catalysis Today》2001,69(1-4):307-314
Deposition of γ-Al2O3 washcoats onto Aluminium and FeCrAlloy® supports in the form of slabs and onto -Al2O3 tubes was performed according to a two-step procedure involving: (a) deposition of a bohemite primer, (b) deposition of the γ-Al2O3 layer, both by dip coating into powder dispersions in HNO3 aqueous solutions. We present herein data concerning the effects of the major preparation variables (HNO3 and H2O concentrations in the dispersion, withdrawal velocity, drying temperature, number of dipping cycles, calcination temperature) on the deposited coating load and on the adherence of the washcoat. Based on a rheological characterisation of the γ-Al2O3 dispersions, we propose also a correlation between their apparent viscosity and the washcoat load. Finally, we briefly illustrate the activity in catalytic combustion reactions of structured Pd-based catalysts prepared according to the investigated washcoating methods. 相似文献
172.
Roberta Paolillo Stefania DApice Gabriele Giacomo Schiattarella Pietro Ameri Domenica Borzacchiello Daniele Catalucci Cristina Chimenti Lia Crotti Sebastiano Sciarretta Daniele Torella Antonio Feliciello Cinzia Perrino 《International journal of molecular sciences》2022,23(14)
Second messenger cyclic adenosine monophosphate (cAMP) has been found to regulate multiple mitochondrial functions, including respiration, dynamics, reactive oxygen species production, cell survival and death through the activation of cAMP-dependent protein kinase A (PKA) and other effectors. Several members of the large family of A kinase anchor proteins (AKAPs) have been previously shown to locally amplify cAMP/PKA signaling to mitochondria, promoting the assembly of signalosomes, regulating multiple cardiac functions under both physiological and pathological conditions. In this review, we will discuss roles and regulation of major mitochondria-targeted AKAPs, along with opportunities and challenges to modulate their functions for translational purposes in the cardiovascular system. 相似文献
173.
Valeria Rella Cinzia Rotondo Alberto Altomare Francesco Paolo Cantatore Addolorata Corrado 《International journal of molecular sciences》2022,23(10)
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by a wide variability of clinical manifestations due to the potential involvement of several tissues and internal organs, with a relapsing and remitting course. Dysregulation of innate and adaptive immune systems, due to genetic, hormonal and environmental factors, may be responsible for a broad spectrum of clinical manifestations, affecting quality of life, morbidity and mortality. Bone involvement represents one of the most common cause of morbidity and disability in SLE. Particularly, an increased incidence of osteoporosis, avascular necrosis of bone and osteomyelitis has been observed in SLE patients compared to the general population. Moreover, due to the improvement in diagnosis and therapy, the survival of SLE patient has improved, increasing long-term morbidities, including osteoporosis and related fractures. This review aims to highlight bone manifestations in SLE patients, deepening underlying etiopathogenetic mechanisms, diagnostic tools and available treatment. 相似文献
174.
Diego Guidolin Cinzia Tortorella Manuela Marcoli Guido Maura Luigi F. Agnati 《International journal of molecular sciences》2022,23(10)
In the last decades, new evidence on brain structure and function has been acquired by morphological investigations based on synergic interactions between biochemical anatomy approaches, new techniques in microscopy and brain imaging, and quantitative analysis of the obtained images. This effort produced an expanded view on brain architecture, illustrating the central nervous system as a huge network of cells and regions in which intercellular communication processes, involving not only neurons but also other cell populations, virtually determine all aspects of the integrative function performed by the system. The main features of these processes are described. They include the two basic modes of intercellular communication identified (i.e., wiring and volume transmission) and mechanisms modulating the intercellular signaling, such as cotransmission and allosteric receptor–receptor interactions. These features may also open new possibilities for the development of novel pharmacological approaches to address central nervous system diseases. This aspect, with a potential major impact on molecular medicine, will be also briefly discussed. 相似文献
175.
Giulia E. Valenti Silvana Alfei Debora Caviglia Cinzia Domenicotti Barbara Marengo 《International journal of molecular sciences》2022,23(11)
In the last few years, antibiotic resistance and, analogously, anticancer drug resistance have increased considerably, becoming one of the main public health problems. For this reason, it is crucial to find therapeutic strategies able to counteract the onset of multi-drug resistance (MDR). In this review, a critical overview of the innovative tools available today to fight MDR is reported. In this direction, the use of membrane-disruptive peptides/peptidomimetics (MDPs), such as antimicrobial peptides (AMPs), has received particular attention, due to their high selectivity and to their limited side effects. Moreover, similarities between bacteria and cancer cells are herein reported and the hypothesis of the possible use of AMPs also in anticancer therapies is discussed. However, it is important to take into account the limitations that could negatively impact clinical application and, in particular, the need for an efficient delivery system. In this regard, the use of nanoparticles (NPs) is proposed as a potential strategy to improve therapy; moreover, among polymeric NPs, cationic ones are emerging as promising tools able to fight the onset of MDR both in bacteria and in cancer cells. 相似文献
176.
Luca Gelsomino Ines Barone Amanda Caruso Francesca Giordano Matteo Brindisi Giovanna Morello Felice Maria Accattatis Salvatore Panza Anna Rita Cappello Daniela Bonofiglio Sebastiano And Stefania Catalano Cinzia Giordano 《International journal of molecular sciences》2022,23(21)
Tumor extracellular vesicles (EVs), as endocytic vesicles able to transport nucleic acids, proteins, and metabolites in recipient cells, have been recognized fundamental mediators of cell-to-cell communication in breast cancer. The biogenesis and release of EVs are highly regulated processes and both the quantity of EVs and their molecular cargo might reflect the metabolic state of the producing cells. We recently demonstrated that the adipokine leptin, whose circulating levels correlate with adipose tissue expansion, is an inducer of EV release from breast cancer cells. Here, we show a specific proteomic signature of EVs released by MCF-7 breast cancer cells grown in the presence of leptin (Lep-EVs), in attempt to find additional molecular effectors linking obesity to breast cancer biology. An analysis of the proteomic profile of Lep-EVs by LC-MS/MS revealed a significant enrichment in biological processes, molecular functions, and cellular components mainly related to mitochondrial machineries and activity, compared to protein content of EVs from untreated breast cancer cells. Metabolic investigations, carried out to assess the autocrine effects of these vesicles on breast cancer cells, revealed that Lep-EVs were able to increase ATP levels in breast cancer cells. This result is associated with increased mitochondrial respiration evaluated by Seahorse analyzer, supporting the concept that Lep-EVs can modulate MCF-7 breast cancer cell oxidative metabolism. Moreover, taking into account the relevance of tumor immune cell crosstalk in the tumor microenvironment (TME), we analyzed the impact of these vesicles on macrophage polarization, the most abundant immune component in the breast TME. We found that tumor-derived Lep-EVs sustain the polarization of M0 macrophages, derived from the human THP-1 monocytic cells, into M2-like tumor-associated macrophages, in terms of metabolic features, phagocytic activity, and increased expression of CD206-positive population. Overall, our results indicate that leptin by inducing the release of EV-enriched in mitochondrial proteins may control the metabolism of MCF-7 breast cancer cells as well as that of macrophages. Characterization of tumor-derived EV protein cargo in an obesity-associated milieu, such as in the presence of elevated leptin levels, might allow identifying unique features and specific metabolic mechanisms useful to develop novel therapeutic approaches for treatment of breast cancer, especially in obese patients. 相似文献
177.
Sonia Bellini Claudia Saraceno Luisa Benussi Andrea Geviti Antonio Longobardi Roland Nicsanu Sara Cimini Martina Ricci Laura Canafoglia Cinzia Coppola Gianfranco Puoti Giuliano Binetti Giacomina Rossi Roberta Ghidoni 《International journal of molecular sciences》2022,23(18)
Emerging data suggest the roles of endo-lysosomal dysfunctions in frontotemporal lobar degeneration (FTLD) and in other dementias. Cathepsin D is one of the major lysosomal proteases, mediating the degradation of unfolded protein aggregates. In this retrospective study, we investigated cathepsin D levels in human plasma and in the plasma small extracellular vesicles (sEVs) of 161 subjects (40 sporadic FTLD, 33 intermediate/pathological C9orf72 expansion carriers, 45 heterozygous/homozygous GRN mutation carriers, and 43 controls). Cathepsin D was quantified by ELISA, and nanoparticle tracking analysis data (sEV concentration for the cathepsin D level normalization) were extracted from our previously published dataset or were newly generated. First, we revealed a positive correlation of the cathepsin D levels with the age of the patients and controls. Even if no significant differences were found in the cathepsin D plasma levels, we observed a progressive reduction in plasma cathepsin D moving from the intermediate to C9orf72 pathological expansion carriers. Observing the sEVs nano-compartment, we observed increased cathepsin D sEV cargo (ng/sEV) levels in genetic/sporadic FTLD. The diagnostic performance of this biomarker was fairly high (AUC = 0.85). Moreover, sEV and plasma cathepsin D levels were positively correlated with age at onset. In conclusion, our study further emphasizes the common occurrence of endo-lysosomal dysregulation in GRN/C9orf72 and sporadic FTLD. 相似文献
178.
Jean-Baptiste Domergue Cinzia Bocca Rosine De Paepe Guy Lenaers Anis M. Limami Guillaume Tcherkez 《International journal of molecular sciences》2023,24(1)
Mitochondrial complex I (CI) plays a crucial role in oxidising NADH generated by the metabolism (including photorespiration) and thereby participates in the mitochondrial electron transfer chain feeding oxidative phosphorylation that generates ATP. However, CI mutations are not lethal in plants and cause moderate phenotypes, and therefore CI mutants are instrumental to examine consequences of mitochondrial homeostasis disturbance on plant cell metabolisms and signalling. To date, the consequences of CI disruption on the lipidome have not been examined. Yet, in principle, mitochondrial dysfunction should impact on lipid synthesis through chloroplasts (via changes in photorespiration, redox homeostasis, and N metabolism) and the endoplasmic reticulum (ER) (via perturbed mitochondrion–ER crosstalk). Here, we took advantage of lipidomics technology (by LC-MS), phospholipid quantitation by 31P-NMR, and total lipid quantitation to assess the impact of CI disruption on leaf, pollen, and seed lipids using three well-characterised CI mutants: CMSII in N. sylvestris and both ndufs4 and ndufs8 in Arabidopsis. Our results show multiple changes in cellular lipids, including galactolipids (chloroplastic), sphingolipids, and ceramides (synthesised by ER), suggesting that mitochondrial homeostasis is essential for the regulation of whole cellular lipidome via specific signalling pathways. In particular, the observed modifications in phospholipid and sphingolipid/ceramide molecular species suggest that CI activity controls phosphatidic acid-mediated signalling. 相似文献
179.
Eliana Gonalves-Alves Marta Garcia Carlos J. Rodríguez-Hernndez Soledad Gmez-Gonzlez Rupert C. Ecker Mariona Suol Oscar Muoz-Aznar Angel M. Carcaboso Jaume Mora Cinzia Lavarino Silvia Mateo-Lozano 《International journal of molecular sciences》2022,23(8)
Neuroblastoma is the most common extracranial solid tumor of childhood, with heterogeneous clinical manifestations ranging from spontaneous regression to aggressive metastatic disease. The calcium-sensing receptor (CaSR) is a G protein-coupled receptor (GPCR) that senses plasmatic fluctuation in the extracellular concentration of calcium and plays a key role in maintaining calcium homeostasis. We have previously reported that this receptor exhibits tumor suppressor properties in neuroblastoma. The activation of CaSR with cinacalcet, a positive allosteric modulator of CaSR, reduces neuroblastoma tumor growth by promoting differentiation, endoplasmic reticulum (ER) stress and apoptosis. However, cinacalcet treatment results in unmanageable hypocalcemia in patients. Based on the bias signaling shown by calcimimetics, we aimed to identify a new drug that might exert tumor-growth inhibition similar to cinacalcet, without affecting plasma calcium levels. We identified a structurally different calcimimetic, AC-265347, as a promising therapeutic agent for neuroblastoma, since it reduced tumor growth by induction of differentiation, without affecting plasma calcium levels. Microarray analysis suggested biased allosteric modulation of the CaSR signaling by AC-265347 and cinacalcet towards distinct intracellular pathways. No upregulation of genes involved in calcium signaling and ER stress were observed in patient-derived xenografts (PDX) models exposed to AC-265347. Moreover, the most significant upregulated biological pathways promoted by AC-265347 were linked to RHO GTPases signaling. AC-265347 upregulated cancer testis antigens (CTAs), providing new opportunities for CTA-based immunotherapies. Taken together, this study highlights the importance of the biased allosteric modulation when targeting GPCRs in cancer. More importantly, the capacity of AC-265347 to promote differentiation of malignant neuroblastoma cells provides new opportunities, alone or in combination with other drugs, to treat high-risk neuroblastoma patients. 相似文献
180.
The surface properties of a Ba-β-alumina sample (Ba ∶ Al ratio 1∶ 12) very stable to sintering (15 m2/g after calcination at 1670 K for 10 h) have been investigated by FT-IR spectroscopy of adsorbed probe molecules (water, pyridine and CO2) and by measuring the isoelectric point. The surface has a medium-strong basic character, justified by the preferential exposition of Ba-O-containing “mirror planes”, parallel to 0001 planes, on the surface of plate-like particles. This behavior has been associated to the resistance to sintering, typical of this material, possibly originated by the difficulty of the very big Ba2+ ions to migrate into the spinel-type alumina blocks. This should imply the blocking of the crystal growth along the crystallographicc axis. 相似文献