Formation of Proto-Kranz in C3 Rice Induced by Spike-Stalk Injection Method

Introduction of C4 photosynthetic traits into C3 crops is an important strategy for improving photosynthetic capacity and productivity. Here, we report the research results of a variant line of sorghum–rice (SR) plant with big panicle and high spikelet density by introducing sorghum genome DNA into rice by spike-stalk injection. The whole-genome resequencing showed that a few sorghum genes could be integrated into the rice genome. Gene expression was confirmed for two C4 photosynthetic enzymes containing pyruvate, orthophosphate dikinase and phosphoenolpyruvate carboxykinase. Exogenous sorghum DNA integration induced a series of key traits associated with the C4 pathway called “proto-Kranz” anatomy, including leaf thickness, bundle sheath number and size, and chloroplast size in bundle sheath cells. Significantly, transgenic plants exhibited enhanced photosynthetic capacity resulting from both photosynthetic CO₂-concentrating effect and improved energy balance, which led to an increase in carbohydrate levels and productivity. Furthermore, such rice plant exhibited delayed leaf senescence. In summary, this study provides a proof for the feasibility of inducing the transition from C3 leaf anatomy to proto-Kranz by spike-stalk injection to achieve efficient photosynthesis and increase productivity.     

The miR-106b/NR2F2-AS1/PLEKHO2 Axis Regulates Migration and Invasion of Colorectal Cancer through the MAPK Pathway

Increasing numbers of miRNAs have been observed as oncogenes or tumor suppressors in colorectal cancer (CRC). It was recently reported that hsa-miR-106b-5p (miR-106b) promoted CRC cell migration and invasion. However, there were also studies showing contradictory results. Therefore, in the present study, we further explore the role of miR-106b and its downstream networks in the carcinogenesis of CRC. We observed that the expression of miR-106b is significantly increased in Pan-Cancer and CRC tissues compared with normal tissues from The Cancer Genome Atlas (TCGA) database. Furthermore, we used Transwell, Cell Counting Kit-8, and colony formation assays to clarify that miR-106b promotes the migratory, invasive, and proliferative abilities of CRC cells. For the first time, we systematically screened the target mRNAs and lncRNAs of miR-106b using TCGA database and the bioinformatics algorithms. Dual-luciferase reporter assay confirmed that NR2F2-AS1 and PLEKHO2 are the direct targets of miR-106b. Furthermore, NR2F2-AS1 acts as a competing endogenous RNA (ceRNA) to regulate PLEKHO2 expression by sponging miR-106b. The results of Gene set enrichment analysis (GSEA) and Western blot indicated that they play important roles in CRC progression by regulating MAPK pathway. Thus, miR-106b/NR2F2-AS1/PLEKHO2/MAPK signaling axis may suggest the potential usage in CRC treatment.     

A Review on the Fundamentals of Palm Oil Fractionation: Processing Conditions and Seeding Agents

Fractionation is a well-established process adopted in the fats and oils industries. It involves the separation of low and high melting triacylglycerol under controlled cooling conditions into olein and stearin fractions with distinct chemical and physical properties. Amongst the other vegetable oils, palm oil is one of the most fractionated oils in the past few decades mainly attributed to its semisolid properties. The various fraction of palm oil allows it to be used in different types of food products such as margarine, frying oil, and cocoa butter substitute. In fractionation, proper control of the fractionation conditions is important to produce the fractions with desirable stearin and olein quality. The purpose of this paper is to critically review the fractionation conditions (crystallization temperature, agitation, cooling rate and crystallization time) that affect the yield and quality of the oil produced. Additionally, it also provides the latest updates on the influence of seeding agents (diacylglycerol, monoacylglycerol, hard fat, phytosterol, phospholipid, lecithin, essential oil, sugar, polyglycerol ester, and talc) used in fractionation. This article is useful to provide a fundamental understanding of fractionation to scientists from the industries or academia working in the fats and oils industries. Practical Applications: This paper provides an in-depth understanding of fractionation particularly on the parameters of fractionation in influencing the quality and yield of the stearin and olein produced. It also for the first time presents the effect of addition of various seeding agents on palm oil fractionation which can help the industry to select the appropriate seeding agents to improve the currently employed fractionation process. Thus, it can act as a guideline for the industry to understand and select the appropriate fractionation conditions when developing a new product using this approach. The fractionation conditions discussed here can also be used as a reference when fractionating other types of fats and oils as most of them share a common background.     

基于时空特性以及需求响应的DG和EV充电站多目标优化配置

针对可再生分布式电源(DG)及电动汽车(EV)大规模接入给配电网带来的用电量增长以及电压波动问题,提出一种基于时空特性以及需求响应的DG和EV充电站多目标协调优化配置方法.通过提取城市路网的拓扑结构,监测路网流量,基于交通规划软件TransCAD进行起讫点(OD)矩阵反推,构建出行概率矩阵以描述用户的出行特性;基于蒙特卡洛方法模拟EV的时空分布特性,考虑EV、DG与常规负荷的时序特性,并基于改进K-means算法构建风-光-负荷的典型运行场景;兼顾电网侧与用户侧,以综合效益、系统负荷波动以及充电耗时成本为目标,构建DG和EV充电站的多目标联合配置模型,并采用改进粒子群优化算法进行求解.结合IEEE 33节点配电网与某城区主干道路网模型进行仿真分析,结果验证了所建模型的有效性与可行性.     

含储能型虚拟同步发电机的直驱风机并网系统自适应协调阻尼控制策略

储能型虚拟同步发电机(VSG)的灵活控制特性能够为风电并网系统提供有效的频率和电压支撑,然而系统振荡特性会受到一定程度的影响,同时风电时变出力特性导致的运行点变化也将使得定参数阻尼控制表现出适应性不足的问题.为此,提出了适应风电出力时变特性的自适应协调阻尼控制策略.首先,在风电并网系统状态空间模型的基础上推导线性变参数模型,以直驱风机的有功功率为调度变量,根据稳定域确定运行空间范围;然后,利用间隙度量对运行空间进行划分,确定各子运行空间的典型运行点并将其作为多胞形顶点,建立调度增益与系统运行工况以及控制器的映射关系;最后,针对不同频率振荡模式协调设计自适应控制器.测试系统的仿真结果表明,所设计的自适应协调阻尼控制器不仅能够同时阻尼含储能型VSG并网系统中的次同步振荡和低频振荡,也能在风电出力大范围时变工况下保持良好的阻尼水平.     

粘结剂对镍基复合粉末及涂层性能影响研究

本文对比分析了四种不同粘结剂制备的镍铬铁铝氮化硼复合粉末及涂层性能, 结果表明： A3 粘结剂的固 化性能和热降解性能与镍铬铁铝氮化硼复合粉末的粉末制备工艺及喷涂工艺匹配性良好, 制备的复合粉末的形貌 和性能较优, 以此制备的封严涂层组织均匀, 硬度为 62 HR15Y, 结合强度为 7.99 MPa, 耐盐浴腐蚀性能良好。     

Zr替代Ti对Ti19．5V40Mn16．2Cr9．8Ni14．5合金的影响Ⅱ：电化学性能

在对合金微观结构和储氢性能研究的基础上，详细考察了少量Zr取代Ti对Ti19．5V40Mn16．2Cr9．8Ni14．5合金电化学性能的影响。通过观察电极的充放电行为，并结合EIS及多种极化曲线的分析结果，发现少量Zr对Ti的取代能够显著提高合金电极的实际电化学容量和循环稳定性，而且还能在一定程度上改善合金的电极动力学性能。对于这些性能的改善，从合金微观结构方面进行了解释。     

应用BP神经网络的单机架可逆式冷轧机弯辊力预设定

针对单机架可逆式冷轧机生产过程中，由于不同热轧生产厂的热轧板形原料性能、板形差别较大、从而造成弯辊力预设定值和实际指标相差较大的问题，利用现场生产工艺数据丰富，采用人工智能BP神经网络的方法，通过大量数据输入进行训练，大大的提高了生产过程中弯辊力预设定值的准确性。     

Ni3Al合金中晶界与位错交互作用的研究

运用嵌入原子法势函数和静态驰豫最陡梯度的计算方法,原子模拟了Ni_3Al合金中晶界与位错的交互作用.主要研究了晶界与位错交互作用的能量特征、晶界结构单元的畸变行为和晶界附近位错的核心结构,并分析讨论了各种因素对它们的影响以及与Ni_3Al晶界韧脆行为之间的关系