基于组态软件的执行系统回转精确度检测系统研究

根据执行系统回转精确度测试实验,利用谐波分析原理,建立了相应的数学模型.基于MCGS组态软件,开发了执行系统回转精确度检测系统,并对测试过程进行了分析.通过实验数据证实,已实现良好的监测效果.     

An Iron Refractory Phenotype in Obese Adipose Tissue Macrophages Leads to Adipocyte Iron Overload

Adipocyte iron overload is a maladaptation associated with obesity and insulin resistance. The objective of the current study was to determine whether and how adipose tissue macrophages (ATMs) regulate adipocyte iron concentrations and whether this is impacted by obesity. Using bone marrow-derived macrophages (BMDMs) polarized to M0, M1, M2, or metabolically activated (MMe) phenotypes, we showed that MMe BMDMs and ATMs from obese mice have reduced expression of several iron-related proteins. Furthermore, the bioenergetic response to iron in obese ATMs was hampered. ATMs from iron-injected lean mice increased their glycolytic and respiratory capacities, thus maintaining metabolic flexibility, while ATMs from obese mice did not. Using an isotope-based system, we found that iron exchange between BMDMs and adipocytes was regulated by macrophage phenotype. At the end of the co-culture, MMe macrophages transferred and received more iron from adipocytes than M0, M1, and M2 macrophages. This culminated in a decrease in total iron in MMe macrophages and an increase in total iron in adipocytes compared with M2 macrophages. Taken together, in the MMe condition, the redistribution of iron is biased toward macrophage iron deficiency and simultaneous adipocyte iron overload. These data suggest that obesity changes the communication of iron between adipocytes and macrophages and that rectifying this iron communication channel may be a novel therapeutic target to alleviate insulin resistance.     

SVD based scale transform invariant observable degree for LTI system

Dear editor, The basic concept of observability is used to express the possibility to recover the state from measurements in modern control theory [1-3].It is a...     

Tumor-Localized Administration of α-GalCer to Recruit Invariant Natural Killer T Cells and Enhance Their Antitumor Activity against Solid Tumors

Invariant natural killer T (iNKT) cells have the capacity to mount potent anti-tumor reactivity and have therefore become a focus in the development of cell-based immunotherapy. iNKT cells attack tumor cells using multiple mechanisms with a high efficacy; however, their clinical application has been limited because of their low numbers in cancer patients and difficulties in infiltrating solid tumors. In this study, we aimed to overcome these critical limitations by using α-GalCer, a synthetic glycolipid ligand specifically activating iNKT cells, to recruit iNKT to solid tumors. By adoptively transferring human iNKT cells into tumor-bearing humanized NSG mice and administering a single dose of tumor-localized α-GalCer, we demonstrated the rapid recruitment of human iNKT cells into solid tumors in as little as one day and a significantly enhanced tumor killing ability. Using firefly luciferase-labeled iNKT cells, we monitored the tissue biodistribution and pharmacokinetics/pharmacodynamics (PK/PD) of human iNKT cells in tumor-bearing NSG mice. Collectively, these preclinical studies demonstrate the promise of an αGC-driven iNKT cell-based immunotherapy to target solid tumors with higher efficacy and precision.     

逆变电源PI双环数字控制技术研究

本文分析了正弦波逆变电源实际应用情况和数字控制的特点,提出了一种带输出电流前馈的PI双环(输出电压外环滤波电感电流内环)数字化控制方案,利用极点配置的方法设计控制系统参数,并根据实际系统模型设计了输出电压和电感电流的状态观测器,最后给出了各种实验条件下的实验波形.实验结果表明,该方案设计简单、实用,能很好地达到逆变电源输出的各项性能指标要求.     

数字图像传感器OV6630应用系统中的抖动问题及解决办法

本文介绍了以图像传感器OV6630、CPLD和C8051F单片机等为核心的图像采集系统,通过实验验证了在CPLD与C8051F单片机接口间存在的信号抖动问题.针对这一问题,根据微分电路原理.采用Verilog-HDL语言设计了去抖动模块,解决了图像采集系统的图像失真问题.     

基于TSIC506的高精度温度检测系统的设计

以ATmega16单片机为控制核心,利用数字化温度传感器TSIC506实现高精度温度检测系统。论述了该系统的硬件组成和软件设计,给出了系统总体结构及相应的ATmega16单片机温度测量程序。实际应用表明,该温度检测系统具有结构简单、成本低、稳定实用等特点,可根据不同需要应用于多种工农业温度检测领域。     

一种抑制时差法超声流量计静态漂移的方法

时差式超声流量计的静态漂移过大会带来动态计量准确度和精度的下降,对接收到的超声信号的准确判断可以有效抑制静态瞬时流量的漂移.传统的阚值法抗干扰能力比较弱,存在较大的静态漂移,严重影响了流量计精度的提升.文中提出过零阈值平滑模型,对阈值法进行改进.实验证明该方法能够准确判断接收到的超声信号,有效抑制了静态瞬时流量的漂移,提高了计量精度.     

双笼翻板施工升降机进楼层存在问题案例分析

施工升降机的布置是一项技术性较强的工作,吊笼进入楼层的方式有是否使用脚手架平台之异。以某住宅项目为例,分析了双笼翻板施工升降机构造与外脚手架类型、建筑物平面与立面造型、施工进度动态管理等方面遇到的问题,提出了缩短附着架长度、调整外脚手架杆件、变更施工方式等解决方案,为今后类似条件下的施工提供借鉴参考。     

TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation

Given that exosomes mediate intercellular communication by delivering cellular components to recipient cells or tissue, they have the potential to be engineered to deliver therapeutic payloads. However, the regulatory mechanism of exosome secretion is poorly understood. In addition, mitochondrial components have been found in exosomes, suggesting communication between mitochondria and exosomes. However, the molecular mechanism of the mitochondria and vesicle interaction remains unclear. Here, we showed that mitochondrial thioredoxin 2 (TRX2) decreased exosome concentrations and inhibited HCT116 cell migration. Coimmunoprecipitation/mass spectrometry (Co-IP/MS) showed that TRX2 interacted with Rab35. TRX2 and Rab35 bound to each other at their N-terminal motifs and colocalized on mitochondria. Furthermore, TRX2 induced Rab35 degradation, resulting in impaired exosome secretion. Additionally, Rab35 mediated the suppressive effects of TRX2 on cell migration, and TRX2 suppressed cell migration through exosomes. Taken together, this study first found an interaction between TRX2 and Rab35. These results revealed a new role for TRX2 in the regulation of exosome secretion and cell migration and explained the upstream regulatory mechanism of Rab35. Furthermore, these findings also provide new molecular evidence for communication between mitochondria and vesicles.