Intra- and inter-laboratory reproducibility of estrogen and progesterone receptor enzyme immunoassay in breast cancer cytosol samples--a Swedish multicenter study. Swedish Society of Cancer Study Group

Estrogen and progesterone receptor analysis results were compared within and between six laboratories in Sweden using frozen breast cancer cytosol samples, and the same technique (enzyme immunoassay, Abbott Laboratories). The concordance in receptor status (positive vs. negative) was excellent (98.4% (571/580)). The discordant results were attributable to values near cut-off (n = 4) or outliers (n = 5), the latter probably being due to analytical errors. One laboratory reported significantly higher ER concentrations than the others; thus caution should be observed when comparing absolute values from different centers. For PgR there were similar differences between the laboratories. However, the intra- and inter-laboratory differences were small compared with the overall variability in ER and PgR content between different samples in a large database. The range of the median intra-laboratory coefficient of variation was 11-23% for ER and 12-19% for PgR, indicating that there is room for improvement in the quality of assay performance.     

Thermal fracture analysis of orthotropic functionally graded materials using an equivalent domain integral approach

A new computational method based on the equivalent domain integral (EDI) is developed for mode I fracture analysis of orthotropic functionally graded materials (FGMs) subjected to thermal stresses. By using the constitutive relations of plane orthotropic thermoelasticity, generalized definition of the J-integral is converted to an equivalent domain integral to calculate the thermal stress intensity factor. In the formulation of the EDI approach, all the required thermomechanical properties are assumed to have continuous spatial variations through the functionally graded medium. Developed methodology is integrated into a fracture mechanics research finite element code FRAC2D using graded finite elements that possess cubic interpolation. Steady-state and transient temperature distribution profiles in orthotropic FGMs are computed using the finite elements based heat transfer analysis software HEAT2D. EDI method is validated and domain independence is demonstrated by comparing the numerical results obtained using EDI to those calculated by an enriched finite element method and to those available in the literature. Single and periodic edge crack problems in orthotropic FGMs are examined in order to study the influences of principal thermal expansion coefficient and thermal conductivity components, relative crack length and crack periodicity on the thermal stress intensity factors. Numerical results show that among the three principal thermal expansion coefficient components, the in-plane component perpendicular to the crack axis has the most significant influence on the mode I stress intensity factor. Gradation profile of the thermal expansion coefficient parallel to the crack axis is shown to have no effect on the outcome of the fracture analysis.     

Aroma volatile compositions of high- and low-aromatic guava varieties

BACKGROUND: The traditional guava variety cultivated in Israel, ‘Ben Dov’, emits a very strong odour, whereas some newly bred varieties have a mild odour. In this study the aroma profile composition of the high‐aromatic ‘Ben Dov’ variety was compared with those of four new low‐aromatic varieties. RESULTS: Overall, using gas chromatography/mass spectrometry, a total of 30 aroma volatiles were detected in fresh ripe guava fruit: 15 of them were specifically detected only in the high‐aromatic ‘Ben Dov’ variety, 13 were detected in both the high‐ and low‐aromatic varieties and two were detected only in the new low‐aromatic varieties. Interestingly, 11 out of the 15 volatiles specifically detected in ‘Ben Dov’ were esters that contribute sweet, tropical and fruity notes. In contrast, ten out of 13 detected terpenes and two detected aldehydes, contributing green, spicy, herbal and woody notes, were common to both the high‐ and low‐aromatic varieties. CONCLUSION: Based on these findings, it is concluded that accumulation of esters is the main reason why the traditional ‘Ben Dov’ guava variety emits such a strong tropical fruity odour. In contrast, the newly bred low‐aromatic guava varieties did not synthesise esters at all and thus lacked fruity aromatic notes. Overall, the results of this study point out the important role of esters in forming tropical fruity guava odours. Copyright © 2011 Society of Chemical Industry     

Effects of Personality on Pair Programming

Personality tests in various guises are commonly used in recruitment and career counseling industries. Such tests have also been considered as instruments for predicting the job performance of software professionals both individually and in teams. However, research suggests that other human-related factors such as motivation, general mental ability, expertise, and task complexity also affect the performance in general. This paper reports on a study of the impact of the Big Five personality traits on the performance of pair programmers together with the impact of expertise and task complexity. The study involved 196 software professionals in three countries forming 98 pairs. The analysis consisted of a confirmatory part and an exploratory part. The results show that: 1) Our data do not confirm a meta-analysis-based model of the impact of certain personality traits on performance and 2) personality traits, in general, have modest predictive value on pair programming performance compared with expertise, task complexity, and country. We conclude that more effort should be spent on investigating other performance-related predictors such as expertise, and task complexity, as well as other promising predictors, such as programming skill and learning. We also conclude that effort should be spent on elaborating on the effects of personality on various measures of collaboration, which, in turn, may be used to predict and influence performance. Insights into such malleable, rather than static, factors may then be used to improve pair programming performance.     

Estimating the Vigdergauz constants for regular hexagonal honeycombs

In this paper we propose a new model for estimating the effective properties of regular hexagonal honeycombs. This model yields some new simple formulae which, in contrast to previous attempts, match the general invariant relations found by Vigdergauz in 1999. By comparing with numerical results, we also find some improved (but still simple) formulae which enable us to estimate the effective properties with an accuracy better than 1%, even for high density honeycombs.     

Determination of flavan-3-ols and trans-resveratrol in grapes and wine using HPLC with fluorescence detection

Concentrations of trans-resveratrol, catechin and epicatechin were analyzed in musts and wines produced from seven red and four white grape cultivars from various wine growing regions of Turkey. Phenolics were quantified using an HPLC method optimized for the separation of wine phenolics. Wine samples contained higher phenolics levels than the corresponding musts. With the exception of Semillion, white wines and musts contained lower concentrations of phenolics than red wines and musts. However, the white cultivar Semillion had the highest concentrations of catechin and epicatechin among all wine and must samples. Semillion wine catechin and epicatechin were 13.7 and 11.8 mg/L, respectively. The highest level of trans-resveratrol among the white cultivars was found in Narince wine (1.93 mg/L). Within the red wine and must cultivars, Bo?azkere, Öküzgozü, and Cabernet contained the highest concentrations of flavan-3-ols and trans-resveratrol. Catechin was the major phenolic in all wines and most musts. Epicatechin was the major phenolic in 6 of the 11 must samples, but none of the wine samples. trans-Resveratrol was generally found in lowest concentrations in both wines and musts.     

The effect of highly purified eicosapentaenoic and docosahexaenoic acids on monocyte phagocytosis in man

The n-3 fatty acids (FA) from marine sources are known to exert antiinflammatory effects on monocyte function. There is still controversy whether n-3 FA may increase the susceptibility to infections. The present study was designed to assess the effect of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHa) on monocyte phagocytosis and respiratory burst activity. Fifty-eight healthy men were randomized to take a daily supplement of 3.8 g highly purified EPA (n=20), 3.6 g DHA (n=19), or corn oil (n=19) for 7 wk. Mononuclear leukocytes were collected, isolated, and cryopreserved prior to and after dietary supplementation. Paired samples were analyzed in the presence of autologous serum in a crossover design. Monocyte phagocytosis and respiratory burst activity were measured by flow cytometry after ingestion of Escherichia coli. Monocytes retained their phagocytic ability and respiratory burst activity after supplementation. No reduction in internalization of bacteria was registered. Dietary n-3 FA and particularly EPA improved bacterial adherence to the monocyte surface. In the crossover experiments, there was an adverse effect of serum enriched with n-3 FA on bacterial adherence. We conclude that monocytes retain their phagocytic potential after supplementation with purified EPA and DHA.