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71.
Daniela T?uber Christian von Borczyskowski 《International journal of molecular sciences》2013,14(10):19506-19525
Single molecule (SM) methods are able to resolve structure related dynamics of guest molecules in liquid crystals (LC). Highly diluted small dye molecules on the one hand explore structure formation and LC dynamics, on the other hand they report about a distortion caused by the guest molecules. The anisotropic structure of LC materials is used to retrieve specific conformation related properties of larger guest molecules like conjugated polymers. This in particular sheds light on organization mechanisms within biological cells, where large molecules are found in nematic LC surroundings. This review gives a short overview related to the application of highly sensitive SM detection schemes in LC. 相似文献
72.
Peptide‐Based Fluorescent Probes for Deacetylase and Decrotonylase Activity: Toward a General Platform for Real‐Time Detection of Lysine Deacylation 下载免费PDF全文
Dr. Debra R. Rooker Yuliya Klyubka Dr. Ritika Gautam Prof. Dr. Elisa Tomat Prof. Dr. Daniela Buccella 《Chembiochem : a European journal of chemical biology》2018,19(5):496-504
Histone deacetylases regulate the acetylation levels of numerous proteins and play key roles in physiological processes and disease states. In addition to acetyl groups, deacetylases can remove other acyl modifications on lysines, the roles and regulation of which are far less understood. A peptide‐based fluorescent probe for single‐reagent, real‐time detection of deacetylase activity that can be readily adapted for probing broader lysine deacylation, including decrotonylation, is reported. Following cleavage of the lysine modification, the probe undergoes rapid intramolecular imine formation that results in marked optical changes, thus enabling convenient detection of deacylase activity with good statistical Z′ factors for both absorption and fluorescence modalities. The peptide‐based design offers broader isozyme scope than that of small‐molecule analogues, and is suitable for probing both metal‐ and nicotinamide adenine dinucleotide (NAD+)‐dependent deacetylases. With an effective sirtuin activity assay in hand, it is demonstrated that iron chelation by Sirtinol, a commonly employed sirtuin inhibitor, results in an enhancement in the inhibitory activity of the compound that may affect its performance in vivo. 相似文献
73.
Daniela Fabricius 《The Journal of Architecture》2013,18(5):812-817
74.
75.
Maria Lisa Garavaglia Daniela Giustarini Graziano Colombo Francesco Reggiani Silvia Finazzi Marta Calatroni Lucia Landoni Nicola Marcello Portinaro Aldo Milzani Salvatore Badalamenti Ranieri Rossi Isabella Dalle-Donne 《International journal of molecular sciences》2022,23(5)
Thiols (sulfhydryl groups) are effective antioxidants that can preserve the correct structure of proteins, and can protect cells and tissues from damage induced by oxidative stress. Abnormal levels of thiols have been measured in the blood of patients with moderate-to-severe chronic kidney disease (CKD) compared to healthy subjects, as well as in end-stage renal disease (ESRD) patients on haemodialysis or peritoneal dialysis. The levels of protein thiols (a measure of the endogenous antioxidant capacity inversely related to protein oxidation) and S-thiolated proteins (mixed disulphides of protein thiols and low molecular mass thiols), and the protein thiolation index (the molar ratio of the S-thiolated proteins to free protein thiols in plasma) have been investigated in the plasma or red blood cells of CKD and ESRD patients as possible biomarkers of oxidative stress. This type of minimally invasive analysis provides valuable information on the redox status of the less-easily accessible tissues and organs, and of the whole organism. This review provides an overview of reversible modifications in protein thiols in the setting of CKD and renal replacement therapy. The evidence suggests that protein thiols, S-thiolated proteins, and the protein thiolation index are promising biomarkers of reversible oxidative stress that could be included in the routine monitoring of CKD and ESRD patients. 相似文献
76.
Giuseppe Donato Mangano Malak Fouani Daniela DAmico Valentina Di Felice Rosario Barone 《International journal of molecular sciences》2022,23(6)
Cachexia is a multifactorial and multi-organ syndrome that is a major cause of morbidity and mortality in late-stage chronic diseases. The main clinical features of cancer-related cachexia are chronic inflammation, wasting of skeletal muscle and adipose tissue, insulin resistance, anorexia, and impaired myogenesis. A multimodal treatment has been suggested to approach the multifactorial genesis of cachexia. In this context, physical exercise has been found to have a general effect on maintaining homeostasis in a healthy life, involving multiple organs and their metabolism. The purpose of this review is to present the evidence for the relationship between inflammatory cytokines, skeletal muscle, and fat metabolism and the potential role of exercise training in breaking the vicious circle of this impaired tissue cross-talk. Due to the wide-ranging effects of exercise training, from the body to the behavior and cognition of the individual, it seems to be able to improve the quality of life in this syndrome. Therefore, studying the molecular effects of physical exercise could provide important information about the interactions between organs and the systemic mediators involved in the overall homeostasis of the body. 相似文献
77.
Maria Chiara Sportelli Margherita Izzi Daniela Loconsole Anna Sallustio Rosaria Anna Picca Roberto Felici Maria Chironna Nicola Cioffi 《International journal of molecular sciences》2022,23(6)
In 2019, the new coronavirus disease (COVID-19), related to the severe acute respiratory syndrome coronavirus (SARS-CoV-2), started spreading around the word, giving rise to the world pandemic we are still facing. Since then, many strategies for the prevention and control of COVID-19 have been studied and implemented. In addition to pharmacological treatments and vaccines, it is mandatory to ensure the cleaning and disinfection of the skin and inanimate surfaces, especially in those contexts where the contagion could spread quickly, such as hospitals and clinical laboratories, schools, transport, and public places in general. Here, we report the efficacy of ZnO nanoparticles (ZnONPs) against SARS-CoV-2. NPs were produced using an ecofriendly method and fully characterized; their antiviral activity was tested in vitro against SARS-CoV-2, showing a decrease in viral load between 70% and 90%, as a function of the material’s composition. Application of these nano-antimicrobials as coatings for commonly touched surfaces is envisaged. 相似文献
78.
Daniela Grimm Herbert Schulz Marcus Krüger Jos Luis Corts-Snchez Marcel Egli Armin Kraus Jayashree Sahana Thomas J. Corydon Ruth Hemmersbach Petra M. Wise Manfred Infanger Markus Wehland 《International journal of molecular sciences》2022,23(6)
Cancer is a disease exhibiting uncontrollable cell growth and spreading to other parts of the organism. It is a heavy, worldwide burden for mankind with high morbidity and mortality. Therefore, groundbreaking research and innovations are necessary. Research in space under microgravity (µg) conditions is a novel approach with the potential to fight cancer and develop future cancer therapies. Space travel is accompanied by adverse effects on our health, and there is a need to counteract these health problems. On the cellular level, studies have shown that real (r-) and simulated (s-) µg impact survival, apoptosis, proliferation, migration, and adhesion as well as the cytoskeleton, the extracellular matrix, focal adhesion, and growth factors in cancer cells. Moreover, the µg-environment induces in vitro 3D tumor models (multicellular spheroids and organoids) with a high potential for preclinical drug targeting, cancer drug development, and studying the processes of cancer progression and metastasis on a molecular level. This review focuses on the effects of r- and s-µg on different types of cells deriving from thyroid, breast, lung, skin, and prostate cancer, as well as tumors of the gastrointestinal tract. In addition, we summarize the current knowledge of the impact of µg on cancerous stem cells. The information demonstrates that µg has become an important new technology for increasing current knowledge of cancer biology. 相似文献
79.
Daniela C.D. da Silva Sonia Letichevsky Luiz E.P. Borges Lucia G. Appel 《International Journal of Hydrogen Energy》2012
The Ni/ZrO2 catalyst is one of the most active systems for the methanation of CO to be employed in the hydrogen purification for PEMFC. This contribution aims to study the effect of ZrO2 on the methanation of CO and CO2. The catalytic behavior of Ni/ZrO2, Ni/SiO2, a physical mixture comprising Ni and ZrO2, and a double-bed reactor were evaluated. The TPD of CO and CO2, TPSR and the cyclohexane dehydrogenation reaction were carried out to describe the catalysts and the reactions. The high activity of Ni/ZrO2 catalyst toward the methanation of CO is related to the presence of active sites on the ZrO2 surface. The methanation of CO occurs on ZrO2 due to its ability to adsorb CO and also because of the hydrogen spillover phenomenon. Apparently, the effect of ZrO2 is less relevant for the methanation of CO2. Ni/ZrO2 is a very promising system for the purification of hydrogen. 相似文献
80.
Daniele Viciani Lorella Dell’Olmo Antonio Gabellini Daniela Gigante Lorenzo Lastrucci 《Landscape Research》2018,43(7):952-964
Widespread trends of abandonment have strongly affected Mediterranean mountains after the Second World War, triggering spontaneous recolonisation of forests. A diachronic analysis of the landscape in a Natura 2000 site (Tuscany, Italy) was carried out using digital aerial photographs (1954, 2013) and a GIS-based methodology, focusing on territories above 1300 m a.s.l. The detected variations of total areas, patch shape, patch dimensions and selected metrics showed a notable shift of the vegetation towards woody types and a drastic reduction of open grasslands, some with high conservation value, accompanied by a strong increase in patch number, surface and edge. A decrease of SDI and SEI diversity indices was also observed. Considering that fragmentation is one of the main causes threatening habitats and species, our results point out a clear necessity for a monitoring programme and suitable actions aimed at improving the status of biodiversity-rich montane grasslands. 相似文献