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M Brown M Webb E Phillips E Skidmore P McIntyre 《Canadian Metallurgical Quarterly》1995,73(7):780-786
We describe the results of functional studies on DNA clones encoding functional bradykinin receptors derived from human, rat, and mouse sources and including both genomic and complementary DNA clones. In both the Xenopus oocyte and the COS cell expression systems, the receptors from human and rat showed the pharmacological properties of B2 receptors, but receptors from mouse displayed both B1- and B2-like pharmacological properties. We further investigated the molecular relationship between the B1 and B2 receptor subtypes expressed by a human fibroblast cell line, and we demonstrate that these two receptor subtypes are encoded by distinct mRNA species. 相似文献
23.
JL Szarek NL Stewart JZ Zhang JA Webb MA Valentovic P Catalano 《Canadian Metallurgical Quarterly》1995,28(2):199-208
Short-term exposure to high concentrations of ozone has been shown to increase airway responsiveness in normal humans and in all laboratory animal species studied to date. While our knowledge concerning the pulmonary effects of single exposures to ozone has increased rapidly over recent years, the effects of repeated exposures are less understood. The goal of the present study was to determine whether airway responsiveness is increased after near-lifetime exposure to ozone. Airway segments representing approximately eighth generation airways were isolated from Fischer 344 rats of both genders that had been exposed for 6 hr per day, 5 days per week for 20 months to 0, 0.12, 0.5, or 1.0 parts per million (ppm) ozone. Circumferential tension development was measured in isolated airways in response to bethanechol, acetylcholine, and electrical field stimulation. Responsiveness of the airways to the contractile stimuli was described by the effective dose or frequency that elicited half-maximum contraction (ED50) and the maximum response. Since ozone exposure is associated with remodeling of peripheral airways, smooth muscle area was determined and tension responses were normalized to the area measurements. Before normalization of tension data to smooth muscle area, neither the ED50 nor maximum response of small bronchi to the contractile stimuli was altered after chronic ozone exposure. Smooth muscle area was greater in airways isolated from animals that had been exposed to 0.5 ppm ozone. After accounting for smooth muscle area, maximum responses of the small bronchi isolated from male rats were significantly reduced after 0.12 and 0.5 ppm ozone. Although not significant statistically, a similar trend was observed in airways isolated from female rats. These results suggest that the increase in airway responsiveness associated with acute ozone exposure does not persist during near-lifetime exposure. Although the mechanism responsible for the adaptation to the effects of O3 on airway responsiveness is unknown, the results indicate that smooth muscle cell function was compromised by the chronic exposure. The mechanism(s) responsible for mediating this effect and the relevance of these results to humans remains to be determined. 相似文献
24.
The electromagnetic fields at reentrant edges made of conductor are generally singular and the polynomial basis functions used in traditional finite elements do not model the fields well in these regions. Two new tetrahedral elements are introduced with basis functions that incorporate the known asymptotic variation of the fields with distance from the edge. The basis is split into gradient and rotational parts. Additional vertex functions can be added to improve the conditioning of the global matrix system that arises in the time-harmonic case. Results are presented for the scattering parameters of a number of waveguide discontinuities. The new elements reduce the error considerably compared to the Whitney element, and generally outperform the second-order edge element, even though that has more degrees of freedom 相似文献
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BACKGROUND: While monotherapy has significant limitations in bipolar disorder, few published data addressing alternatives exist. Treatment algorithms have been proposed, but none have undergone empirical evaluation. This study provides a systematic prospective, open evaluation of the effectiveness and tolerability of a treatment algorithm for patients with histories of mania. METHOD: Twenty-eight symptomatic outpatients from a public mental health facility who were diagnosed as having either bipolar I or schizoaffective illness, bipolar type, entered the study. Minimum blood levels of lithium and divalproex sodium were defined. Medications were pushed to predetermined levels (as tolerated) before proceeding to the next algorithm step. Clinical symptoms were assessed monthly using the Brief Psychiatric Rating Scale (BPRS, 27 item) and Clinical Global Impressions scale. RESULTS: Pretreatment and posttreatment clinical symptoms were compared. Over 50% of patients attained 30% improvement from baseline BPRS after 4 months. Thirty-six percent of patients (N = 10) became mood stable, 46% (N = 13) remained mood unstable, and 18% (N = 5) dropped out before completing the algorithm. Although patients who finished the algorithm were taking more medication, either dosage and/or drugs, somatic complaints did not increase. CONCLUSION: The potential benefit of a defined treatment algorithm was demonstrated for these complex and persistently ill patients. Despite long treatment histories, patients improved with more frequent visits and addition of medication(s). A randomized controlled trial comparing a similar treatment algorithm with treatment-as-usual is warranted. 相似文献
27.
J Whittle CJ Lin JR Lave MJ Fine KM Delaney DZ Joyce WW Young WN Kapoor 《Canadian Metallurgical Quarterly》1998,36(7):977-987
OBJECTIVES: The authors describe the relation of provider characteristics to processes, costs, and outcomes of medical care for elderly patients hospitalized for community-acquired pneumonia. METHODS: Using Medicare claims data, Medicare beneficiaries discharged from Pennsylvania hospitals during 1990 with community-acquired pneumonia were identified. Claims data were used to ascertain mortality, readmissions, use of procedures and physician consultations, and the costs of care. The relationship of these measures to provider characteristics was analyzed using regression techniques to adjust for patient characteristics, including comorbidity and microbial etiology. RESULTS: Among 22,294 pneumonia episodes studied, 30-day mortality was 17.0%. After adjusting for patient characteristics, 30-day mortality and readmission rates were unrelated to hospital teaching status or urban location or to physician specialty. Use of procedures and physician consultations was more common and costs were 11% higher among patients discharged from teaching hospitals compared with nonteaching hospitals. Similarly, costs were 15% higher at urban hospitals compared with rural hospitals. General internists and medical subspecialists used more procedures and had higher costs than family practitioners. CONCLUSIONS: Processes and costs of care for community-acquired pneumonia varied by provider characteristics, but neither mortality nor readmission rates did. These differences cannot be explained by clinical variables in the database. Further studies should determine whether less costly patterns of care for pneumonia, and perhaps other conditions, could replace more costly ones without compromising patient outcomes. 相似文献
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Direct reconstruction in three dimensions for two-dimensional projection data has been achieved by cone-beam reconstruction techniques. In this paper explicit formulas for a cone-beam convolution and back-projection reconstruction algorithm are given in a form which can be easily coded for a computer. The algorithm is justified by analyzing tomographic reconstructions of a uniformly attenuating sphere from simulated noisy projection data. A particular feature of this algorithm is the use of a one-dimensional rather than two-dimensional convolution function, greatly speeding up the reconstruction. The technique is applicable however large the cone angle of data capture and correctly reduces to the pure fan-beam reconstruction technique in the central section of the cone. The method has been applied to data captured on a cone-beam CT scanner designed for bone mineral densitometry. 相似文献
30.
An Objective Method to Prioritize Socio‐Environmental Water Management Tradeoffs Using Multi‐Criteria Decision Analysis
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Rivers provide many social and environmental services that benefit humanity. A critical role of water mangers is to prioritize water allocation options that trade off socio‐economic and hydro‐ecological benefits in rivers. Methods for multi‐criteria decision analysis (MCDA) provide a structured and systematic manner for researchers to aid in this process. In this paper, we describe a new MCDA method that prioritizes large multi‐dimensional sets of tradeoffs to support well‐informed water management in rivers. The method was developed based on an environmental flows planning study in the Goulburn‐Broken River catchment, Victoria, Australia. A combined simulation and heuristic optimization procedure was previously integrated into a hydrological catchment network model. That process resulted in a large set of viable daily water allocation schedules that traded off long‐term irrigation and hydro‐ecological benefits at the catchment outlet. We provided new guidance procedures to identify priority tradeoffs that can be used in stakeholder deliberations and catchment decision‐making. Our MCDA method included combined multi‐dimensional ordination and cluster analysis to spread the water allocation alternatives onto a two‐dimensional plane to discover alternatives with similar criteria tradeoffs. A geometric distance‐based method was performed on the full set of alternatives and on the identified clusters to rank the alternatives in accordance with minimizing the distance of the alternatives to an ideal but non‐feasible reference point in multi‐dimensional space. This method complements the use of elicitation procedures when water manager or other stakeholder interaction is not an option or when objectivity is desired. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献