Chromosome translocation based on illegitimate recombination in human tumors

Recurrent chromosome translocations in nonhematological tumors are restricted to specific subtypes, and their mechanism is currently unknown. Analysis of the sequence data of 113 interchromosomal junctions derived from 77 Ewing's tumors carrying the characteristic t(11;22) translocation indicate that, in this tumor, translocations are initiated independently on each chromosome in regions that lack site specific recombination signal. Local sequence duplications, deletions, and, most importantly, inversions that are diagnostic of DNA hairpin formation indicate that, at the breakpoint, single-stranded DNA ends are processed individually before interchromosomal joining. Taken together, these observations suggest that chromosome translocations in Ewing's tumors are mediated through a genuine illegitimate recombination mechanism.     

Anti-GM-CSF monoclonal antibody therapy for refractory acute leukemia

Several phase I trials and pilot studies using Monoclonal Antibody (MoAb) have been performed in B-cell neoplasms, but this approach has not until now been extensively tested in myeloid leukemias. Recently, we evaluated the use of anti-Granulocyte-Macrophage Colony-Stimulating Factor MoAb (Anti-GM-CSF MoAb) in acute myeloid leukemia (AML). Eight patients fulfilled inclusion criteria and received a single course of Anti-GM-CSF MoAb infusion during 5 to 15 days. Anti-GM-CSF MoAb was well tolerated and was detectable in pharmacokinetics studies. Using Human Anti-Rat Antibodies (HARA), we also observed an immunological response to the MoAb. Despite sufficient levels detected in the serum and biological activity of Anti-GM-CSF MoAb in vivo, no anti-leukemic effect was noted, except for one patient who had a decrease of 50% in the marrow blast cell mass. These observations indicate that leukemic proliferation in vivo involves a complex network spanning many mechanisms, and inhibition of leukemia is not effective if only one of these key targets is attacked. The development of these new approaches may be more effective in the future.     

Application of the Whittle Estimator to the Modeling of Traffic Based on the M/G/∞ Process

Several traffic measurements reports have shown the presence of persistent correlations in current networ Its. The essence of this behavior can be captured by several classes of stochastic processes. The M/G/infin process plays a key role for this purpose. In addition to its theoretical simplicity, it has been successfully used to model widely different traffic sources. In this letter, we obtain the parametric form of the spectral density of an M/G/infin-based process, able to model both the long-term and the short-term correlation behavior of traffic, and we check the robustness of the Whittle estimator taking this process as the underlying one.     

Efficient use of side information in multiple-antenna datatransmission over fading channels

We derive performance limits for two closely related communication scenarios involving a wireless system with multiple-element transmitter antenna arrays: a point-to-point system with partial side information at the transmitter, and a broadcast system with multiple receivers. In both cases, ideal beamforming is impossible, leading to an inherently lower achievable performance as the quality of the side information degrades or as the number of receivers increases. Expected signal-to-noise ratio (SNR) and mutual information are both considered as performance measures. In the point-to-point case, we determine when the transmission strategy should use some form of beamforming and when it should not. We also show that, when properly chosen, even a small amount of side information can be quite valuable. For the broadcast scenario with an SNR criterion, we find the efficient frontier of operating points and show that even when the number of receivers is larger than the number of antenna array elements, significant performance improvements can be obtained by tailoring the transmission strategy to the realized channel     

Neural networks for variational problems in engineering

In this work a conceptual theory of neural networks (NNs) from the perspective of functional analysis and variational calculus is presented. Within this formulation, the learning problem for the multilayer perceptron lies in terms of finding a function, which is an extremal for some functional. Therefore, a variational formulation for NNs provides a direct method for the solution of variational problems. This proposed method is then applied to distinct types of engineering problems. In particular a shape design, an optimal control and an inverse problem are considered. The selected examples can be solved analytically, which enables a fair comparison with the NN results. Copyright © 2008 John Wiley & Sons, Ltd.     

Comparison of system performance at 50, 62.5 and 100 GHz channel spacing over transoceanic distances at 40 Gbit/s channel rate using RZ-DPSK

Assuming RZ-DPSK format at 40 Gbit/s channel rate, the impact on the Q-factor of a variable channel spacing has been evaluated in five WDM experiments conducted in a recirculating loop over transoceanic distances. A degradation of the Q-factor by nearly 3 dB is observed when the channel spacing is reduced from 100 to 50 GHz     

Reducing hysteresis in magnetostrictive-piezoelectric magneticsensors

In this paper, we present a method to avoid hysteresis in magnetostrictive-piezoelectric sensors. It is based on the application of an alternating magnetic field of a frequency different than the piezoelectric exciting frequency that supplies enough energy to overcome any domain wall pinning. The signal is filtered with a notch filter synchronized to the magnetic field frequency and thus it does not contribute to the useful response of the sensor. The results show an appreciable decrease in the magnetic hysteresis and also a small decrease in the sensitivity of the sensor     

Searching for Lyme disease in Colombia: a preliminary study on the vector

Since ageing is accompanied by various alterations of the immune system, the aim of the present study was to investigate the effect of age on the expression of surface markers in peripheral blood monocytes. We studied 28 healthy young subjects and 28 elderly subjects fulfilling the criteria of the SENIEUR protocol. Peripheral blood mononuclear cells were isolated and the expression of various surface markers was analysed by double colour flow cytometry. The mean fluorescence intensity of the intercellular adhesion molecule- (CD54), CD29, CD45RO and CD32 was increased significantly in CD14+ cells of elderly people when compared with the young subjects. No significant differences were found in the expression of CD11a, CD11b, CD15, CD26, CD27, CD33, CD45RA, CD45RB, CD49d, HLA-DR and CD65. In summary, we demonstrated significant increase in four monocyte surface markers in subjects of old age; this finding may be related to certain immune phenomena observed in ageing subjects such as auto-immunity.     

Differential colocalization of estrogen receptor beta (ERbeta) with oxytocin and vasopressin in the paraventricular and supraoptic nuclei of the female rat brain: an immunocytochemical study

Evidence exists for the localization of the newly identified estrogen receptor beta (ERbeta) within the rat paraventricular nucleus (PVN) and supraoptic nucleus (SON), regions which lack ERalpha. Presently, we investigate whether ERbeta-like-immunoreactivity (-ir) is found within cells of several major neuropeptide systems of these regions. Young adult Sprague-Dawley rats were ovariectomized (OVX), and 1 week later half of the animals received estradiol-17beta (E). Dual-label immunocytochemistry was performed on adjacent sections by using an ERbeta antibody, followed by an antibody to either oxytocin (OT), arginine-vasopressin (AVP), or corticotropin releasing hormone. Nuclear ERbeta-ir was identified within SON and retrochiasmatic SON, and in specific PVN subnuclei: medial parvicellular part, ventral and dorsal zones, dorsal and lateral parvicellular parts, and in the posterior magnocellular part, medial and lateral zones. However, the ERbeta-ir within magnocellular areas was noticeably less intense. OT-/ERbeta-ir colocalization was confirmed in neurons of the parvicellular subnuclei, in both OVX and OVX+E brains ( approximately 50% of OT and 25% of ERbeta-labeled cells between bregma -1.78 and -2.00). In contrast, few PVN parvicellular neurons contained both AVP- and ERbeta-ir. As well, very little overlap was observed in the distribution of cells containing corticotropin releasing hormone- or ERbeta-ir. In the SON, most nuclear ERbeta-ir colocalized with AVP-ir, whereas few OT-/ERbeta-ir dual-labeled cells were observed. These findings suggest that estrogen can directly modulate specific OT and AVP systems through an ERbeta-mediated mechanism, in a tissue-specific manner.