Liposome and lipid bilayer arrays towards biosensing applications

Sensitive and selective biosensors for high-throughput screening are having an increasing impact in modern medical care. The establishment of robust protein biosensing platforms however remains challenging, especially when membrane proteins are involved. Although this type of proteins is of enormous relevance since they are considered in >60% of the pharmaceutical drug targets, their fragile nature (i.e., the requirement to preserve their natural lipid environment to avoid denaturation and loss of function) puts strong additional prerequisites onto a successful biochip. In this review, the leading approaches to create lipid membrane-based arrays towards the creation of membrane protein biosensing platforms are described. Liposomes assembled in micro- and nanoarrays and the successful set-ups containing functional membrane proteins, as well as the use of liposomes in networks, are discussed in the first part. Then, the complementary approaches to create cell-mimicking supported membrane patches on a substrate in an array format will be addressed. Finally, the progress in assembling free-standing (functional) lipid bilayers over nanopore arrays for ion channel sensing will be reported. This review illustrates the rapid pace by which advances are being made towards the creation of a heterogeneous biochip for the high-throughput screening of membrane proteins for diagnostics, drug screening, or drug discovery purposes.     

Biosensing by Densely Packed and Optically Coupled Plasmonic Particle Arrays

Densely packed plasmonic particle arrays are investigated for biosensing applications. Such particle arrays exhibit interparticle optical coupling creating a strong field between the particles, which is useful for sensing purposes. The sensor properties, such as bulk sensitivity, layer sensitivity, and the depth of sensitivity are investigated with the aid of a multiple multipole program. Sensitivity to the analyte with low concentration is also examined by a dynamic adsorption processes. The detectable concentration limit of streptavidin within 3000 s in the detection system is expected from the signal‐to‐noise to be less than 150 pM .     

Megaloblastic anaemia in childhood due to vitamin B12 deficiency, report of 3 cases of congenital selective vitamin B12 malabsorption (author''s tranls)

Three cases of congenital selective malabsorption of vitamin B12 (Imerslund-Grasbeck syndrome) are presented. Pathophysiological aspected and clinical symptoms of this disease are discussed together with other megaloblastic anaemias in childhood caused by vitamin B12 deficiency.     

Non‐destructive assessment of retaining wall of former coal mine plant

A large retaining wall (appr. 9 m high and 286 m long) of a former coal mine has been investigated using Ground Penetrating Radar (GPR), with two low frequency antennas (200 and 400 MHz), and Large Aperture UltraSound (LAUS) to gather information about the condition and inner structure of the wall. Three vertical lines were selected in representative areas of the wall to evaluate the usefulness of Non‐Destructive Testing (NDT) with these two methods. The LAUS results showed the layer structure at one line where the wall was enforced by a concrete shell and were unspecific regarding the inner structure beyond the first layer. GPR results were able to be collected much faster and showed some internal features. Penetration was limited to 2–3 m due to the high absorption in the material.     

Microwave-Assisted Synthesis of Glycoconjugates by Transgalactosylation with Recombinant Thermostable β-Glycosidase from Pyrococcus

The potential of the hyperthermophilic β-glycosidase from Pyrococcus woesei (DSM 3773) for the synthesis of glycosides under microwave irradiation (MWI) at low temperatures was investigated. Transgalactosylation reactions with β-N-acetyl-d-glucosamine as acceptor substrate (GlcNAc-linker-tBoc) under thermal heating (TH, 85 °C) and under MWI at 100 and 300 W resulted in the formation of (Galβ(1,4)GlcNAc-linker-tBoc) as the main product in all reactions. Most importantly, MWI at temperatures far below the temperature optimum of the hyperthermophilic glycosidase led to higher product yields with only minor amounts of side products β(1,6-linked disaccharide and trisaccharides). At high acceptor concentrations (50 mM), transgalactosylation reactions under MWI at 300 W gave similar product yields when compared to TH at 85 °C. In summary, we demonstrate that MWI is useful as a novel experimental set-up for the synthesis of defined galacto-oligosaccharides. In conclusion, glycosylation reactions under MWI at low temperatures have the potential as a general strategy for regioselective glycosylation reactions of hyperthermophilic glycosidases using heat-labile acceptor or donor substrates.     

Nanomechanical study of polycarbonate/boehmite nanoparticles/epoxy ternary composite and their interphases

Thermoplastic modified thermosets are of great interest especially due to their improved fracture toughness. Comparable enhancements have been achieved by adding different nanofillers including inorganic particles such as nanosized boehmite. Here, we present a nanomechanical study of two composite systems, the first comprising a polycarbonate (PC) layer in contact with epoxy resin (EP) and the second consisting of a PC layer containing boehmite nanoparticles (BNP) which is also in contact with an EP layer. The interaction between PC and EP monomer is tested by in situ Fourier transformed infrared (FT-IR) analysis, from which a reaction induced phase separation of the PC phase is inferred. Both systems are explored by atomic force microscopy (AFM) force spectroscopy. AFM force-distance curves (FDC) show no alteration of the mechanical properties of EP at the interface to PC. However, when a PC phase loaded with BNP is put in contact with an epoxy system during curing, a considerable mechanical improvement exceeding the rule of mixture was detected. The trend of BNP to agglomerate preferentially around EP dominated regions and the stiffening effect of BNP on EP shown by spatial resolved measurements of Young's modulus, suggest the effective presence of BNP within the EP phase.     

Pseudohomophone effects in lexical decision: Still a challenge for current word recognition models.

Computational models that implement a serial mechanism of phonological assembly predict interactions between the size of the pseudohomophone (PsH) effect and stimulus length. Models with frequency-sensitive word representations predict baseword frequency effects. These predictions were tested in a lexical-decision task. The results showed constant PsH effects across different word lengths (in favor of parallel phonological activation) and baseword frequency effects (in favor of frequency-sensitive representations). However, the baseword frequency effect was opposite of what the models predicted. This result is most easily accommodated by models that assume an orthographic verification mechanism. The plausibility of such a mechanism was further supported by the results of 2 additional experiments investigating the effects of response speed and spelling probability (feedback consistency) on the size of the PsH effect. (PsycINFO Database Record (c) 2010 APA, all rights reserved)