Predicting precipitate particle breakage in a pipeline: Effect of agitation intensity during precipitate formation

This paper describes the breakage behaviour of whey protein aggregates formed under different agitation intensities when flowing turbulently through a pipe. The experimental results show that particles formed at lower agitation intensity remain larger along the pipeline even after long exposure time to turbulent conditions than those particles formed at higher agitation rates. This suggests that the agitation rate during the formative stages of precipitates has important effect on the size and perhaps on the structure of aggregates which in turn determine their flow behaviour under turbulent conditions. It is postulated that larger aggregate cores were formed at low agitation which were hardly broken during precipitate transportation.A predictive model that relates particle breakage with the local dissipation rate (ε_i) in the pipe, the maximum or threshold dissipation rate (ε_th) that a particle with a given size can withstand before any disruption occurs and the residence time of the particle in the pipeline (t) was used to model the break-up of the precipitates. The profile of the turbulent eddy dissipation rate (ε_i) along the experimental flow geometry was described using the results of CFD simulations which in turn were run using the standard κ-ε turbulent model. The breakage model was improved by introducing a new term which accounts for the restructuring process during the aggregate formation stage. The predicted results agree well with the experimental data allowing one to conclude that the particle size reduction was well coupled with the variation of ε along the turbulent flow path.     

Walnut consumption in a weight reduction intervention: effects on body weight,biological measures,blood pressure and satiety

Background

Dietary strategies that help patients adhere to a weight reduction diet may increase the likelihood of weight loss maintenance and improved long-term health outcomes. Regular nut consumption has been associated with better weight management and less adiposity. The objective of this study was to compare the effects of a walnut-enriched reduced-energy diet to a standard reduced-energy-density diet on weight, cardiovascular disease risk factors, and satiety.

Methods

Overweight and obese men and women (n =?100) were randomly assigned to a standard reduced-energy-density diet or a walnut-enriched (15% of energy) reduced-energy diet in the context of a behavioral weight loss intervention. Measurements were obtained at baseline and 3- and 6-month clinic visits. Participants rated hunger, fullness and anticipated prospective consumption at 3 time points during the intervention. Body measurements, blood pressure, physical activity, lipids, tocopherols and fatty acids were analyzed using repeated measures mixed models.

Results

Both study groups reduced body weight, body mass index and waist circumference (time effect p <?0.001 for each). Change in weight was ?9.4 (0.9)% vs. -8.9 (0.7)% (mean [SE]), for the standard vs. walnut-enriched diet groups, respectively. Systolic blood pressure decreased in both groups at 3 months, but only the walnut-enriched diet group maintained a lower systolic blood pressure at 6 months. The walnut-enriched diet group, but not the standard reduced-energy-density diet group, reduced total cholesterol and low-density lipoprotein cholesterol (LDL-C) at 6 months, from 203 to 194 mg/dL and 121 to 112 mg/dL, respectively (p <?0.05). Self-reported satiety was similar in the groups.

Conclusions

These findings provide further evidence that a walnut-enriched reduced-energy diet can promote weight loss that is comparable to a standard reduced-energy-density diet in the context of a behavioral weight loss intervention. Although weight loss in response to both dietary strategies was associated with improvements in cardiovascular disease risk factors, the walnut-enriched diet promoted more favorable effects on LDL-C and systolic blood pressure.

Trial registration

The trial is registered at (NCT02501889).

     

Lake Sammamish response to wastewater diversion and increasing urban runoff

Lake Sammamish has shown a decrease in its mean annual concentration of phosphorus following diversion of about one third of the external loading in 1968. During 1971–1975 the P concentration averaged 27 μg l⁻¹, in contrast to the prediversion (1964–1966) concentration of 33 μg l⁻¹, and may be equilibrating near the predicted steady state concentration of 22 μg l⁻¹. Neither phytoplankton biomass or Secchi visibility has changed following diversion, however the blue green component of the phytoplankton decreased by nearly 50%. The failure of biomass and visibility to improve is probably a result of similar pre- and postdiversion winter spring epilimnetic P concentrations. The marked reduction in P since diversion occurred during and prior to fall overturn and may have represented a supply of P for later summer early fall blue green algal populations that declined after diversion.Runoff from a rapidly developing westside portion (18%) of the watershed is contributing substantially to P loading of the lake. Development to a density of about ten dwellings ha⁻¹ has increased loading possibly on the order of 14%. Future development of the eastside portion (26% of watershed) may increase loading by 20% and be equivalent to nearly one half of the P previously diverted in 1968.     

Cloning and characterization of a eukaryotic pantothenate kinase gene (panK) from Aspergillus nidulans

Pantothenate kinase (PanK) is the key regulatory enzyme in the CoA biosynthetic pathway. The PanK gene from Escherichia coli (coaA) has been previously cloned and the enzyme biochemically characterized; highly related genes exist in other prokaryotes. We isolated a PanK cDNA clone from the eukaryotic fungus Aspergillus nidulans by functional complementation of a temperature-sensitive E. coli PanK mutant. The cDNA clone allowed the isolation of the genomic clone and the characterization of the A. nidulans gene designated panK. The panK gene is located on chromosome 3 (linkage group III), is interrupted by three small introns, and is expressed constitutively. The amino acid sequence of A. nidulans PanK (aPanK) predicted a subunit size of 46.9 kDa and bore little resemblance to its bacterial counterpart, whereas a highly related protein was detected in the genome of Saccharomyces cerevisiae. In contrast to E. coli PanK (bPanK), which is regulated by CoA and to a lesser extent by its thioesters, aPanK activity was selectively and potently inhibited by acetyl-CoA. Acetyl-CoA inhibition of aPanK was competitive with respect to ATP. Thus, the eukaryotic PanK has a distinct primary structure and unique regulatory properties that clearly distinguish it from its prokaryotic counterpart.     

Thermal stability and mechanical behavior of cycloaliphatic–DGEBA epoxy blend system initiated by cationic latent catalyst

The effect of the composition of an epoxy blend based on a cycloaliphatic (CAE) and diglycidyl ether of bisphenol A (DGEBA) epoxides containing N‐benzylpyrazinium hexafluoroantimonate (BPH) as a thermal or UV latent initiator was investigated in the context of their thermal stability and mechanical properties. The compositions of a CAE–DGEBA blend were varied within 100:0, 80:20, 60:40, 20:80, and 0:100 by mole percent. Latent properties were measured by the degree of conversion. As a result, the thermal stability characterized from the initial decomposition temperature (IDT), the temperature of maximum weight loss (T_max), the integral procedural decomposition temperature (IPDT), and the decomposition activation energies by TGA increased when the DGEBA composition was increased. According to the mechanical measurements, the flexural and tensile strengths increased with an increase of the DGEBA composition because of the compact hydrogen bond, long repeat unit, and bulky side groups of the DGEBA, while both the elastic and tensile moduli decreased. This latter result was attributed to the DGEBA intermolecular interaction, resulting in a toughened three‐dimensional network, which dispersed the internal stress. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 78: 290–297, 2000     

Novel Acyl Phosphate Mimics that Target PlsY,an Essential Acyltransferase in Gram‐Positive Bacteria

PlsY is a recently discovered acyltransferase that executes an essential step in membrane phospholipid biosynthesis in Gram‐ positive bacteria. By using a bioisosteric replacement approach to generate substrate‐based inhibitors of PlsY as potential novel antibacterial agents, a series of stabilized acyl phosphate mimetics, including acyl phosphonates, acyl α,α‐difluoromethyl phosphonates, acyl phosphoramides, reverse amide phosphonates, acyl sulfamates, and acyl sulfamides were designed and synthesized. Several acyl phosphonates, phosphoramides, and sulfamates were identified as inhibitors of PlsY from Streptococcus pneumoniae and Bacillus anthracis. As anticipated, these inhibitors were competitive inhibitors with respect to the acyl phosphate substrate. Antimicrobial testing showed the inhibitors to have generally weak activity against Gram‐positive bacteria with the exception of some acyl phosphonates, reverse amide phosphonates, and acyl sulfamates, which had potent activity against multiple strains of B. anthracis.     

Directionality effect on mechanical properties of 3D n-directional braided (SiO2)f/SiO2 composites prepared by silica sol-infiltration-sintering method

Three-dimensional (3D) and n-directional (n = 4, 5, 6 and 7) woven (SiO₂)_f/SiO₂ composites were prepared by silica sol-infiltration-sintering (SIS) method. All the woven reinforced structures were of the same fiber content, and the prepared samples were cut in the same manner parallel to the respective braiding direction. The effect of n-direction on mechanical properties has been studied by conducting tensile, flexural and shear tests. The results showed that flexural and shear strengths of the samples increased gently with increasing n-direction from 4 to 7. However, with increasing n-direction, tensile strength initially increased steeply for 4–5 n-directions, followed by a gradual decrease for 6–7 n-directions. 3D 5-directional sample had the maximum tensile strength while 3D 7-directional sample had the maximum flexural strength and shear strength.     

FPGA-based Novel Adaptive Scheme Using PN Sequences for Self-Calibration and Self-Testing of MEMS-based Inertial Sensors

We propose a novel adaptive technique based on pseudo-random (PN) sequences for self-calibration and self-testing of MEMS-based inertial sensors (accelerometers and gyroscopes). The method relies on using a parameterized behavioral model implemented on FPGA, whose parameters values are adaptively tuned, based on the response to test pseudo-random actuation of the physical structure. Dedicated comb drives actuate the movable mass with binary maximum length pseudo-random sequences of small amplitude, to keep the device within the linear operating regime. The frequency of the stimulus is chosen within the mechanical spectral operating range of the micro-device, such that the induced response leads to the identification of the mechanical transfer function, and to the tuning of the associated digital behavioral model. In case of a micro-gyroscope, experimental results demonstrate the adaptive tracking of the damping coefficient from 5.57?×?10^?5? Kg/s to 7.12?×?10^?5? Kg/s and of the stiffness coefficient from 132?N/m to 137.7?N/m. In the case of a MEMS accelerometer, the damping and stiffness coefficients are correctly tracked from 3.4?×?10^?3? Kg/s and 49.56?N/m to 4.57?×?10^?3? Kg/s and 51.48?N/m, respectively—the former values are designer-specified target values, while the latter are experimentally measured parameters for fabricated devices operating in a real environment. Hardware resources estimation confirms the small area the proposed algorithm occupies on the targeted FPGA device.     

Biofilm growth and characteristics in an alternating pumped sequencing batch biofilm reactor (APSBBR)

A novel biofilm reactor-alternating pumped sequencing batch biofilm reactor (APSBBR)-was developed to treat synthetic dairy wastewater at a volumetric chemical oxygen demand (COD) loading rate of 487 g COD m(-3) d(-1) and an areal loading rate of 5.4 g COD m(-2) d(-1). This biofilm reactor comprised two tanks, Tanks 1 and 2, with two identical plastic biofilm modules in each tank. The maximum volume of bulk fluid in the two-tank reactor was the volume of one tank. The APSBBR was operated as a sequencing batch biofilm reactor with five operational phases-fill (25 min), anoxic (9 h), aerobic (9 h), settle (6 h) and draw (5 min). The fill, anoxic, settle and draw phases occurred in Tank 1. In the aerobic phase, the wastewater was circulated between the two tanks with centrifugal pumps and aeration was mainly achieved through oxygen absorption by micro-organisms in the biofilms when they were exposed to the air. In this paper, the biofilm growth and characteristics in the APSBBR were studied in a 98-day laboratory-scale experiment. During the course of the study, it was found that the biofilm thickness (delta) in Tank 1 ranged from 1.2 to 7.2 mm and that in Tank 2 from 0.5 to 2.2 mm; the biofilm growth against time (t) can be simulated as delta=0.07t0.99 (R2 = 0.97, P = 0.002) in Tank 1 and delta = 0.08t0.66 (R2 = 0.81, P = 0.04) in Tank 2. The biomass yield coefficient, Y, was 0.18 g volatile solids (VS) g(-1) COD removal. The biofilm density in both tanks, X, decreased as the biofilm thickness increased and can be correlated to the biofilm thickness, delta .