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121.
Aging is characterized by increase in reactive oxygen (ROS) and nitrogen (RNS) species, key factors of cardiac failure and disuse-induced muscle atrophy. This study focused on serum nitroproteome as a trait of longevity by adopting two complementary gel-based techniques: two-dimensional differential in gel electrophoresis (2-D DIGE) and Nitro-DIGE coupled with mass spectrometry of albumin-depleted serum of aged (A, n = 15) and centenarian (C, n = 15) versus young females (Y, n = 15). Results indicate spots differently expressed in A and C compared to Y and spots changed in A vs. C. Nitro-DIGE revealed nitrosated protein spots in A and C compared to Y and spots changed in A vs. C only (p-value < 0.01). Nitro-proteoforms of alpha-1-antitripsin (SERPINA1), alpha-1-antichimotripsin (SERPINA3), ceruloplasmin (CP), 13 proteoforms of haptoglobin (HP), and inactive glycosyltransferase 25 family member 3 (CERCAM) increased in A vs. Y and C. Conversely, nitrosation levels decreased in C vs. Y and A, for immunoglobulin light chain 1 (IGLC1), serotransferrin (TF), transthyretin (TTR), and vitamin D-binding protein (VDBP). Immunoblottings of alcohol dehydrogenase 5/S-nitrosoglutathione reductase (ADH5/GSNOR) and thioredoxin reductase 1 (TRXR1) indicated lower levels of ADH5 in A vs. Y and C, whereas TRXR1 decreased in A and C in comparison to Y. In conclusion, the study identified putative markers in C of healthy aging and high levels of ADH5/GSNOR that can sustain the denitrosylase activity, promoting longevity.  相似文献   
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125.
An energy-based control for an n-H-bridges multilevel active rectifier   总被引:1,自引:0,他引:1  
This paper deals with the control of a multilevel n-H-bridges front-end rectifier. This topology allows n distinct dc buses to be fed by the same ac source offering a high loading flexibility suitable for traction applications as well as for industrial automation plants. However, this flexibility can lead the system to instability if the dc buses operate at different voltage levels and with unbalanced loads. Thus, linear controllers, designed on the basis of the small-signal linearization, are not effective any longer and stability can not be ensured as large-signal disturbances occur. The use of a passivity-based control (PBC) designed via energy considerations and without small-signal linearization properly fits stability problems related to this type of converter. The system has been split into n subsystems via energy considerations in order to achieve the separate control of each dc bus and its stability in case of load changes or disturbances generated by other buses. Then, a set of n passivity-based controllers (one for each subsystem) is adopted: the controllers are linked using dynamical parameters computed through energy balance equations. Hence, the system dc buses are independent and stable as experimental results demonstrate.  相似文献   
126.
The surface tension and the viscosity of a series of industrial alloys have been measured by the oscillating drop technique with an electromagnetic levitation device under reduced gravity conditions in several parabolic flights. It was demonstrated that the 20 seconds of reduced gravity available in a parabola were sufficient for melting, heating into the liquid phase, and cooling to solidification of typically 7 mm diameter metallic specimen. The surface tension and the viscosity were obtained from the frequency and the damping time constant of the oscillation which were evaluated from the temperature signal of a highresolution pyrometer. Alloys processed included steels, Ni-based superalloys, and Ti-alloys which were supplied by industrial partners to the project. Three to four parabolas were sufficient to obtain the surface tension and the viscosity over a large range in temperature.  相似文献   
127.
The development of reliable numerical models permits to investigate the manufacturing processes with very low incremental costs or prototyping efforts hence it provides a relevant help in process optimisation and gives great opportunity for making maximum use of sparse process data [Shercliff HR, Lovatt AM. Selection of manufacturing process in design and the role of process modelling. Prog Mater Sci 2001;46:429–59].

Among others the metal forming processes have heavily benefited from the finite element numerical computing technology [Chenot JL, Massoni E. Finite element modelling and control of new metal forming processes. Int J Machine Tool Manuf 2006;46:1194–200].

The self piercing riveting (SPR) is a cold forming process which creates a strong mechanical interlock between two or more sheets by means of a semi-tubular rivet, which, pressed by a punch, pierces the upper sheet and flares into the bottom one. It is governed by complex multiphysics phenomena whose governing equations can be resolved using the finite element method.

In this paper all the governing equations are fully reported along with the mathematics of the resolving method needed for setting up and simulate a finite element model of the self piercing riveting of an aluminium alloy. A case study of the SPR of two sheets of the 6060T4 aluminium alloy using a steel rivet was investigated. The calculations were performed using the LsDyna finite element commercial code. The problems encountered and the solutions applied for the preparation of the model and the run of the calculation were presented and discussed. The obtained results were validated by comparison with data coming from a laboratory experiment.  相似文献   

128.
1. Polymorphonuclear leukocytes (PMN) may contribute to the pathogenesis of acute coronary heart disease (CHD). 2. Epidemiological and laboratory evidence suggests that red wine, by virtue of its polyphenolic constituents, may be more effective than other alcoholic beverages in reducing the risk of CHD mortality. 3 The aim of the present study was to investigate the effects of trans-resveratrol (3,4',5-trihydroxy-trans-stilbene), a polyphenol present in most red wines, on functional and biochemical responses of PMN, upon in vitro activation. 4. trans-Resveratrol exerted a strong inhibitory effect on reactive oxygen species produced by PMN stimulated with 1 microM formyl methionyl leucyl phenylalamine (fMLP) (IC50 1.3+/-0.13 microM, mean+/-s.e.mean), as evaluated by luminol-amplified chemiluminescence. 5. trans-Resveratrol prevented the release of elastase and beta-glucuronidase by PMN stimulated with the receptor agonists fMLP (1 microM, IC50 18.4+/-1.8 and 31+/-1.8 microM), and C5a (0.1 microM, IC50 41.6+/-3.5 and 42+/-8.3 microM), and also inhibited elastase and beta-glucuronidase secretion (IC50 37.7+/-7 and 25.4+/-2.2 microM) and production of 5-lipoxygenase metabolites leukotriene B4 (LTB4), 6-trans-LTB4 and 12-trans-epi-LTB4 (IC50 48+/-7 microM) by PMN stimulated with the calcium ionophore A23187 (5 microM). 6. trans-Resveratrol significantly reduced the expression and activation of the beta2 integrin MAC-1 on PMN surface following stimulation, as revealed by FACS analysis of the binding of an anti-MAC-1 monoclonal antibody (MoAb) and of the CBRM1/5 MoAb, recognizing an activation-dependent epitope on MAC-1. Consistently, PMN homotypic aggregation and formation of mixed cell-conjugates between PMN and thrombin-stimulated fixed platelets in a dynamic system were also prevented by transresveratrol. 7. These results, indicating that trans-resveratrol interferes with the release of inflammatory mediators by activated PMN and down-regulates adhesion-dependent thrombogenic PMN functions, may provide some biological plausibility to the protective effect of red wine consumption against CHD.  相似文献   
129.
A purine nucleoside phosphorylase from Aeromonas hydrophyla (AhPNP) was covalently immobilized in a pre‐packed stainless steel column containing aminopropylsilica particles via Schiff base chemistry upon glutaraldehyde activation. The resulting AhPNP‐IMER (Immobilized Enzyme Reactor, immobilization yield ≈50%) was coupled on‐line through a 6‐way switching valve to an HPLC apparatus containing an analytical or a semi‐preparative chromatographic column. The synthesis of five 6‐modified purine ribonucleosides was carried out by continuously pumping the reaction mixture through the AhPNP‐IMER until the highest conversion was reached, and then directing the reaction mixture to chromatographic separation. The conditions of the AhPNP‐catalyzed transglycosylations (2:1 ratio sugar donor:base acceptor; 10 mM phosphate buffer; pH 7.5; temperature 37 °C, flow rate 0.5 mL min−1) were optimized by a fractional factorial experimental design. Coupling the bioconversion step with the product purification in such an integrated platform resulted in a fast and efficient synthetic process (yield=52–89%; <10 mg) where sample handling was minimized. To date, AhPNP‐IMER has retained completely its activity upon 50 reactions in 10 months.

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130.
The H-bridge-based multilevel active rectifier is an attractive topology that allows feeding of multiple dc loads. The main control problem is to manage state variables (one current plus dc voltages) with only switching functions. In this paper, three PI-based solutions are discussed, and the tuning of the best solution is addressed in case resonant controllers are used instead of classical integrators. Moreover, practical problems such as the implementation and the influence of phase-locked-loop systems and of analog filters (first order and Butterworth) on the measurements are discussed.  相似文献   
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