Prevalence, distribution, and diversity of Listeria monocytogenes in retail environments, focusing on small establishments and establishments with a history of failed inspections

Despite growing concerns about cross-contamination of ready-to-eat foods with Listeria monocytogenes, our knowledge about the ecology and transmission of L. monocytogenes in retail establishments has remained limited. We conducted a cross-sectional study to characterize the prevalence, distribution, and subtype diversity of L. monocytogenes in 120 New York State retail deli establishments that were hypothesized to present an increased risk for environmental L. monocytogenes contamination (i.e., small establishments and establishments with a history of failed New York State Agriculture and Markets inspections). Analysis of these data along with previously reported data for 121 predominantly larger retail establishments in New York State identified establishment size, geographic location, and inspection history as significant predictors of L. monocytogenes presence and prevalence. The odds of an establishment being L. monocytogenes positive were approximately twice as high for large establishments, establishments located in New York City, or establishments with poor inspection history (as compared with establishments without these attributes), even though correlation between location and inspection history complicated interpretation of results. Within an establishment, L. monocytogenes was significantly more prevalent on nonfood contact surfaces than on food contact surfaces; prevalence was particularly high for floors and in floor drains, sinks, the dairy case, and milk crates. L. monocytogenes subtype diversity differed between sites, with lineage I isolates significantly associated with nonfood contact surfaces and lineage II isolates significantly associated with food contact surfaces. Isolates belonging to the same ribotype were often found dispersed across multiple sites within an operation.     

3D Active Edge Silicon Detector Tests With 120 GeV Muons

3D detectors with electrodes penetrating through the silicon wafer and covering the edges were tested in the SPS beam line X5 at CERN in autumn 2003. Detector parameters including efficiency, signal-to-noise ratio, and edge sensitivity were measured using a silicon telescope as a reference system. The measured sensitive width and the known silicon width were equal within less than 10 mum.     

Microclimatic modeling of the urban thermal environment of Singapore to mitigate urban heat island

This study investigates the urban heat island effect in Singapore and examines the key factors causing this effect. The possibilities of improving heat extraction rate by optimizing air flow in selected hot spots were explored. The effect of building geometry, façade materials and the location of air-conditioning condensers on the outdoor air temperature was explored using computational fluid dynamics (CFD) simulations. It was found that at very low wind speeds, the effect of façade materials and their colours was very significant and the temperature at the middle of a narrow canyon increased up to 2.5 °C with the façade material having lower albedo. It was also found that strategically placing a few high-rise towers will enhance the air flow inside the canyon thereby reducing the air temperature. Adopting an optimum H/W ratio for the canyons increased the velocity by up to 35% and reduced the corresponding temperature by up to 0.7 °C.     

Design of biorefinery systems for conversion of corn stover into biofuels using a biorefinery engineering framework

Unlocking the potential and value of lignocellulosic residues is an important step in making biorefineries economically and environmentally promising. This calls for a holistic and systematic approach in designing sustainable industrial systems. In this work, biorefinery systems via biochemical route (acetone–butanol–ethanol or ABE system) and thermochemical route (gasification and mixed alcohols or GMA system) for converting corn stover into biofuels have been designed using a Sustainable Engineering Framework. The framework involves eight main steps: (1) design problem definition, (2) data collection, (3) process synthesis and simulation, (4) process integration, (5) resource recovery from residues, (6) utility system design, (7) economic and environmental modelling and (8) economic value and environmental impact margin analysis for decision making. Consideration of resource recovery from biorefinery waste streams has proven to be the key in making biorefineries self-sustaining and with low environmental impacts. Simultaneous economic and environmental feasibility assessment at the early stage of process design is highly envisaged. The cost of biofuel production in the ABE system has been found to be 49.2 US$/GJ and 69.9 US$/GJ in the GMA system. The greenhouse gas emissions are 46.2 g CO₂-eq/GJ for ABE and 19.0 g CO₂-eq/GJ for GMA, lower than gasoline (85 g CO₂-eq/GJ). The GMA system is not economically compelling though with high environmental benefit, while the ABE system has shown to be both economically and environmentally feasible.     

Tunable Spectrum Selectivity for Multiphoton Absorption with Enhanced Visible Light Trapping in ZnO Nanorods

Observation of visible light trapping in zinc oxide (ZnO) nanorods (NRs) correlated to the optical and photoelectrochemical properties is reported. In this study, ZnO NR diameter and c‐axis length respond primarily at two different regions, UV and visible light, respectively. ZnO NR diameter exhibits UV absorption where large ZnO NR diameter area increases light absorption ability leading to high efficient electron–hole pair separation. On the other hand, ZnO NR c‐axis length has a dominant effect in visible light resulting from a multiphoton absorption mechanism due to light reflection and trapping behavior in the free space between adjacent ZnO NRs. Furthermore, oxygen vacancies and defects in ZnO NRs are associated with the broad visible emission band of different energy levels also highlighting the possibility of the multiphoton absorption mechanism. It is demonstrated that the minimum average of ZnO NR c‐axis length must satisfy the linear regression model of Z _p,min = 6.31d to initiate the multiphoton absorption mechanism under visible light. This work indicates the broadening of absorption spectrum from UV to visible light region by incorporating a controllable diameter and c‐axis length on vertically aligned ZnO NRs, which is important in optimizing the design and functionality of electronic devices based on light absorption mechanism.     

台湾的“化妆间”与“垃圾不落地”

正趁着春节假期,我到台北和高雄看望久别的姐妹们。虽是第一次来到宝岛,不过吃住都是在姐妹家中,这趟旅行倒像是回家一样,没什么违和感与陌生感,然而小新鲜却总是不断。刚下飞机时,看到几乎人人戴口罩,这吓了我一跳,其实那里空气真不错,没遇到过雾霾天。这是怎么回事呢?     

Bacterium‐Templated Polymer for Self‐Selective Ablation of Multidrug‐Resistant Bacteria

The recognition and inactivation of specific pathogenic bacteria remain an enormous scientific challenge and an important therapeutic goal. Therefore, materials that can selectively target and kill specific pathogenic bacteria, without harming beneficial strains are highly desirable. Here, a material platform is reported that exploits bacteria as a template to synthesize polymers with aggregation‐induced emission (AIE) characteristic by copper‐catalyzed atom transfer radical polymerization for self‐selective killing of the bacteria that templates them with no antimicrobial resistance. The bacteria‐templated polymers show very weak fluorescence in aqueous media, however, the fluorescence is turned on upon recognition of the bacteria used as the template to synthesize the polymer even at a low concentration of 600 ng mL^?1. Moreover, the incorporated AIE fluorogens (AIEgens) can act as an efficient photosensitizer for reactive oxygen species (ROS) generation after bacteria surface binding, which endows the templated polymers with the capability for selective bacterial killing. The bacterium‐templated synthesis is generally applicable to a wide range of bacteria, including clinically isolated multidrug‐resistant bacterial strains. It is envisioned that the bacterium‐templated method provides a new strategy for bacteria‐specific diagnostic and therapeutic applications.