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51.
Automated Buildup of Biomimetic Films in Cell Culture Microplates for High‐Throughput Screening of Cellular Behaviors 下载免费PDF全文
Paul Machillot Catarina Quintal Fabien Dalonneau Loic Hermant Pauline Monnot Kelsey Matthews Vincent Fitzpatrick Jie Liu Isabelle Pignot‐Paintrand Catherine Picart 《Advanced materials (Deerfield Beach, Fla.)》2018,30(27)
An automatic method is established for layer‐by‐layer (LbL) assembly of biomimetic coatings in cell culture microplates using a commercial liquid‐handling robot. Highly homogeneous thin films are formed at the bottom of each microwell. The LbL film‐coated microplates are compatible with common cellular assays, using microplate readers and automated microscopes. Cellular adhesion is screened on crosslinked and peptide‐functionalized LbL films and stem cell differentiation in response to increasing doses of bone morphogenetic proteins (2, 4, 7, 9). This method paves the way for future applications of LbL films in cell‐based assays for regenerative medicine and high‐throughput drug screening. 相似文献
52.
Abhilash Sreekumar Savvas P. Triantafyllou François-Xavier Bécot Fabien Chevillotte 《International journal for numerical methods in engineering》2020,121(8):1791-1821
We introduce a novel heterogeneous multiscale method for the elastic analysis of two-dimensional domains with a complex microstructure. To this end, the multiscale finite element method is revisited and originally upgraded by introducing virtual element discretizations at the microscale, hence allowing for generalized polygonal and nonconvex elements. The microscale is upscaled through the numerical evaluation of a set of multiscale basis functions. The solution of the equilibrium equations is performed at the coarse scale at a reduced computational cost. We discuss the computation of the multiscale basis functions and corresponding virtual projection operators. The performance of the method in terms of accuracy and computational efficiency is evaluated through a set of numerical examples. 相似文献
53.
Florian Mitjana Sonia Cafieri Florian Bugarin Christian Gogu Fabien Castanie 《工程优选》2019,51(1):140-159
Structural optimization is of increasing interest in a wide variety of application fields. In this article, structural optimization under stress and buckling constraints is investigated. A structure comprised of a set of frame elements is considered. The aim is to obtain the minimal mass structure, by optimizing the number of frame elements and their cross sectional dimensions. A formulation as a mixed-integer nonlinear optimization problem with a tailored objective function is introduced. This cost function is a combination of the structural mass and the sum of the second moments of inertia of each structural element. Moreover, a new algorithm, tailored to the considered problem, is proposed. Numerical results show that the proposed approach provides interesting structural mass savings. 相似文献
54.
Fabien Cremona Alo Laas Toomas Kiv Margot Sepp Peeter Nges Tiina Nges 《Lakes & Reservoirs: Research and Management》2019,24(1):18-23
Theoretical pelagic primary production of phytoplankton and benthic primary production of periphyton were modelled for two small lakes in Estonia (Northeast Europe). Although located only 500 m apart, the water colour and light attenuation of these two lakes differed markedly. The Secchi depth (SD) in the clear‐water lake was 4.5 m and only 0.47 m in the dark‐water lake. The total phosphorus (TP) concentrations were, respectively, 15 μg/l and 28 μg/l. An empirical model whose inputs were morphometric, light conditions and dissolved organic carbon parameters obtained from in situ measurements was employed for the present study. The model calculated primary production with a time‐step of 10 min, and a spatial resolution of 10 cm, from sunrise to sunset and from lake surface to lake bottom. The primary production of periphyton and phytoplankton was almost equal in the clear lake, whereas only phytoplankton contributed to whole‐lake primary production in the dark lake because of the stronger light attenuation in the water column. The results of the present study indicated the depth‐distribution profiles differed dramatically between the two lakes. The clear lake had a deep, U‐shaped curve, with the productive layer reaching considerable depth soon after sunrise and maintaining a similar profile throughout the light hours. In contrast, the dark lake production declined rapidly with increasing depth, whereas the profile changed over the day reaching the greatest depth at noon. 相似文献
55.
Vernotte Alexandre Cretin Aymeric Legeard Bruno Peureux Fabien 《International Journal on Software Tools for Technology Transfer (STTT)》2022,24(2):127-158
International Journal on Software Tools for Technology Transfer - The ADS-B—automatic dependent surveillance-broadcast—technology requires aircraft to broadcast their position and... 相似文献
56.
Structure and analgesic properties of layered double hydroxides intercalated with low amounts of ibuprofen 下载免费PDF全文
Robson Sousa Jenny Jouin Olivier Masson Fabien Remondiere Alex Lemarchand Maggy Colas Philippe Thomas Jerônimo Lameira Gilmara N. T. Bastos Anderson B. Lima José L. M. Nascimento Marcos Anicete‐Santos Waldinei R. Monteiro Cláudio N. Alves 《Journal of the American Ceramic Society》2017,100(6):2712-2721
Ibuprofen‐intercalated layered double hydroxides (LDH‐IBU) have been successfully synthesized via a coprecipitation method with a nominal [Al3+]/[Mg2+] ratio of 0.5 and a variable molar IBU/([Al3+]+[Mg2+]) ratio of 0, 0.15, 0.18, 0.24, 0.36, and 0.72. After an accurate determination of the composition, the nature of the intercalated species and the effective intercalation yield from to IBU, it is shown that the synthesis route used allows a good control of the quantity of intercalated IBU within the LDH framework. This results in different samples with full or partial IBU intercalation in the interlayer space in exchange of nitrate anions. The analysis of the X‐ray diffraction basal reflections reveals that the intercalation of IBU in the framework only increases the basal distances with no alteration of the brucite‐type layers. Also, a computational study used to model the positions and shapes of the basal reflections showed that the structure of the nonfully intercalated compounds follows a random interstratification scheme. Finally, three samples ranging from slightly to fully IBU‐intercalated galleries were selected for preliminary in vivo assays. These tests showed a strong tendency that after 24 hours the low yield of IBU‐intercalated compounds are almost as efficient as the fully intercalated sample. 相似文献
57.
We report an experimental investigation of the electrokinetic properties and size variations of four F-specific bacteriophages of the types MS2, GA, Qbeta and SP (21-30 nm in diameter) over a broad range of pH values (1.5-7.5) and NaNO3 electrolyte concentrations (1-100 mM). The results obtained by dynamic light scattering show that the aggregation of SP and GA particles takes place over the whole range of pH and ionic strength conditions examined. For MS2 phages, the aggregation of MS2 particles is not observed for pH higher than the isoelectric point (pI) and large ionic strengths for which interparticular repulsive electrostatic interactions are however expected to be sufficiently screened. Aggregation of the MS2 phages, dispersed in 1 and 100 mM electrolyte concentration, occurs at pH 4, which basically corresponds to the pI as determined by electrophoresis measurements. The Qbeta particles suspended in solutions of low electrolyte concentrations aggregate at low pH values (pI approximately 3) and, unlike MS2, at large ionic strengths over the whole range of pH conditions considered in this study. These elements allow the determination of the hydrophobic sequence for the four phages SP approximately GA>Qbeta>MS2. Close inspection of the electrokinetic results reveals small to significant variations of the pI values-depending on the phage considered-with respect to the concentration of indifferent NaNO3 electrolyte. This indicates that features other than chemical and electrostatic in nature play a key role in determining the pI and more generally the electrophoretic mobility mu of viral particles. A qualitative interpretation is given and is based on the consideration of inner electro-osmotic flow within the isolated or aggregated particles. The impact of the flow properties within the particles is further in agreement with recent theoretical formalism developed for the electrokinetics of soft multiplayer particles, the phages analyzed here being some illustrative examples. The determination and qualitative interpretation of the surface properties of the viral particles as reported in the current study are commented within the context of water treatment especially concerning viral removal by membrane filtration processes. 相似文献
58.
Fabien Choffat Paul Smith Walter Caseri 《Advanced materials (Deerfield Beach, Fla.)》2008,20(11):2225-2229
Organometallic polymers comprising a backbone of covalently connected metal atoms can be regarded as molecular metal wires surrounded by a jacket of organic matter. Such polymers are rare and their materials properties are largely unexplored. Here, we report on polystannanes, (SnR2)n, that is, polymers with a backbone of tin atoms, which are synthesized by dehydropolymerization of dialkylstannanes (H2SnR2) with the catalyst [RhCl(PPh3)3]. The polystannanes feature reversible phase transitions into liquid‐crystalline states, remarkably, even below room temperature, and, interestingly, oriented either parallel or perpendicular to external driving forces, depending on the length of the alkyl substituents. 相似文献
59.
Eva Monroy Fabien Guillot Sylvain Leconte Laurent Nevou Laetitia Doyennette Maria Tchernycheva Francois H. Julien Esther Baumann Fabrizio R. Giorgetta Daniel Hofstetter 《Journal of Materials Science: Materials in Electronics》2008,19(8-9):821-827
In this work, we summarize the latest progress in intersubband devices based on GaN/AlN nanostructures for operation in the near-infrared. We first discuss the growth and characterization of ultra-thin GaN/AlN quantum well and quantum dot superlattices by plasma-assisted molecular-beam epitaxy. Then, we present the performance of nitride-based infrared photodetectors and electro-optical modulators operating at 1.55 μm. Finally, we discuss the progress towards intersubband light emitters, including the first experimental observation of intersubband photoluminescence in nitride nanostructures. 相似文献
60.
Sampling for metabolome analysis of microorganisms 总被引:6,自引:0,他引:6
In the present work we investigated the most commonly applied methods used for sampling of microorganisms in the field of metabolomics in order to unravel potential sources of error previously ignored but of utmost importance for accurate metabolome analysis. To broaden the significance of our study, we investigated different Gram-negative and Gram-positive bacteria, i.e., Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Gluconobacter oxydans, Pseudomonas putida, and Zymononas mobilis, and analyzed metabolites from different catabolic and anabolic intracellular pathways. Quenching of cells with cold methanol prior to cell separation and extraction led to drastic loss (>60%) of all metabolites tested due to unspecific leakage. Using fast filtration, Gram-negative bacteria also revealed a significant loss (>80%) when inappropriate washing solutions with low ionic strength were applied. Adapting the ionic strength of the washing solution to that of the cultivation medium could almost completely avoid this problem. Gram-positive strains did not show significant leakage independent of the washing solution. Fast filtration with sampling times of several seconds prior to extraction appears to be a suitable approach for metabolites with relatively high intracellular level and low turnover such as amino acids or TCA cycle intermediates. Comparison of metabolite levels in the culture supernatant and the cell interior revealed that the common assumption of whole broth quenching protocols attributing the metabolites found exclusively to the intracellular pools may not be valid in many cases. In such cases a differential approach correcting for medium-contained metabolites is required. 相似文献