High yield gold nanoparticle‐based DNA isolation method for human papillomaviruses genotypes from cervical cancer tissue samples

Gold nanoparticles (AuNPs) are commonly used in biosensors of various kinds. However, its application to extract DNA from cancer tissues has not been extensively studied. The purification of DNA from cancer tissues is an important step in diagnostic and therapeutic development. Almost, all cervical cancer cases are associated with high‐risk human papillomavirus (HR‐HPV) infection. Accurate viral diagnosis has so far relied on the extraction of adequate amounts of DNA from formalin‐fixed, paraffin‐embedded (FFPE) tissue samples. Till now, no specific and sensitive DNA purification method has been introduced for the extraction of HR‐HPV from FFPE tissue. Since the commercially available purification kits are not sensitive and specific enough for HR‐HPV DNA targets, in this study, a DNA purification method was designed based on AuNPs to purify sufficient amounts of HR‐HPV DNA from cervical cancer tissue samples. AuNPs were coated with a series of oligonucleotide probes to hybridize to specific DNA sequences of HR‐HPV genotypes. Results showed that 733 out of 800 copies of type‐specific HPV DNA were recovered with complete specificity, compared to 36 copies with a standard commercial kit (Qiagen FFPE). The high yield of DNA (91.6%) is the main advantage of the AuNPs‐probe purification method.Inspec keywords: cancer, biological tissues, microorganisms, gold, DNA, lab‐on‐a‐chip, gynaecology, purification, biochemistry, molecular biophysics, nanoparticles, nanomedicine, cellular biophysicsOther keywords: specific extraction method, cervical cancer tissue samples, high‐risk human papillomavirus infection, paraffin‐embedded tissue samples, specific DNA purification method, sensitive DNA purification method, FFPE tissue, HR‐HPV DNA targets, specific DNA sequences, HR‐HPV genotypes, type‐specific HPV DNA, AuNP‐probe purification method, gold nanoparticle‐based DNA isolation method, oligonucleotide, human papillomaviruse genotypes, clinical samples, Au     

Corrosion Inhibition of Carbon Steel in Aqueous HCl Solutions by Acid Pre-Magnetization Technique: Experimental Study and Modelling

Protection of Metals and Physical Chemistry of Surfaces - The effect of magnetization of acid before usage (pre-magnetization technique) on the corrosion inhibition of carbon steel (CS) in 7.5, 10...     

Rapid determination of soil unconfined compressive strength using reflectance spectroscopy

Bulletin of Engineering Geology and the Environment - Understanding the physical and especially mechanical properties of forest soils is very important in forest engineering operations including...     

Optimal placement of steel plate shear walls for steel frames by bat algorithm

Layout optimization of steel frames with steel plate walls (SPWs) using a meta‐heuristic search algorithm is the main aim of the present study. SPWs are lateral load‐resisting systems, especially against earthquake excitation. These systems offer significant advantages in terms of cost, performance and ease of design compared with other systems. In this study, orthotropic membrane model is used to model the behaviour of steel plate shear walls. The newly developed bat algorithm, which is based on the echolocation behaviour of bats, is employed as the present study optimizer. Design variables of the optimization problem consist of the cross sections of beams and columns of the frame, the web plate thicknesses of SPWs and the placement of SPW in the frame. The bat algorithm performs suitable selection of sections from the AISC wide‐flange (W) shapes list. Strength constraints of the American Institute of Steel Construction Load and Resistance Factor Design and displacement constraints are checked during the optimization process. The results reveal the effectiveness of the proposed method for optimization of steel frames with SPWs. Copyright © 2014 John Wiley & Sons, Ltd.     

Probiotic-Induced Tolerogenic Dendritic Cells: A Novel Therapy for Inflammatory Bowel Disease?

Inflammatory bowel diseases (IBDs) are immune-mediated, chronic relapsing diseases with a rising prevalence worldwide in both adult and pediatric populations. Treatment options for immune-mediated diseases, including IBDs, are traditional steroids, immunomodulators, and biologics, none of which are capable of inducing long-lasting remission in all patients. Dendritic cells (DCs) play a fundamental role in inducing tolerance and regulating T cells and their tolerogenic functions. Hence, modulation of intestinal mucosal immunity by DCs could provide a novel, additional tool for the treatment of IBD. Recent evidence indicates that probiotic bacteria might impact immunomodulation both in vitro and in vivo by regulating DCs’ maturation and producing tolerogenic DCs (tolDCs) which, in turn, might dampen inflammation. In this review, we will discuss this evidence and the mechanisms of action of probiotics and their metabolites in inducing tolDCs in IBDs and some conditions associated with them.     

A study on the cure characteristics of epoxy/diaminodiphenylmethane system prepared at room temperature

The curing behavior and kinetics of epoxy resin with diaminodiphenylmethane (DDM) as the curing agent was studied by many researchers, however all of them prepared the system at a high‐temperature condition (i.e., T ≥ 80°C). In this study, a mixture of epoxy/DDM was prepared at ambient temperature and its curing characteristics were studied by using differential scanning calorimetry (DSC). The autocatalytic model was used to calculate the kinetic factors in the dynamic experiments. The kinetics of the curing reaction was also evaluated by two different isoconversional models; namely Friedman method and the Advanced Isoconversional method proposed by Vyazovkin to investigate the activation energy behavior during the curing reaction. The activation energy of the curing reaction was found to be in the range of 48 ± 2 kJ/mol and might be considered to be constant during the curing. In fact, our findings were different from the result reported by other researchers for the system which was prepared at elevated temperature. Therefore, it seems that the preparation temperature of the samples influenced considerably on the curing behavior of epoxy with DDM. Finally, a time–temperature–transformation (TTT) diagram was established to determine the cure process and glass transition properties of the system. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

Pomegranate fallen leaves as a source of natural dye for mordant-free dyeing of wool

The application of metal mordants is usually necessary in dyeing of wool with natural dyes to improve the dye exhaustion and fastness properties. The majority of metal salts generally used as mordants are considered as toxic and it is important to find replacements for them. Plant sources with high content of tannins are good candidates as bio-mordant or colourant to overcome this drawback. In this study, the waste fallen leaves of pomegranate tree were used as a source of natural dye for the eco-friendly dyeing of wool fabric without the use of metal mordants. The dyeing process variables including dye concentration, dyebath pH, and temperature were optimised using response surface methodology to obtain the highest colour strength. The colour strength was increased by increasing the natural dye powder up to 100%owf while the optimum pH and dyebath temperature were 4 and 100°C, respectively. The sample dyed under the optimal condition exhibited good fastness properties against washing and light. This study approved the potential of Punica granatum fallen leaves for the dyeing of wool without any mordant, while high fastness properties were obtained.     

Experimental investigation of the effect of vibration on mechanical properties of 304 stainless steel welded parts

Some of the problems that occur during the welding process include the creation of coarse grains in the weld structure and the hardening of the weld region, which reduce the strength and impact resistance of the welded parts. One technique to improve the mechanical properties of weld is the application of mechanical vibration to the molten pool. In this article, the effect of vibrating the part during welding on the mechanical properties of steel plates has been investigated in the tungsten inert gas (TIG) welding process. The plate is made of stainless steel 304 with 2 mm in thickness. A filler material has also been used for welding so that the effect of vibration can be observed on the weld pool region. The experimental tests have been performed under different welding conditions with respect to voltage, current, welding speed, vibrations amplitude, and frequency. Then, the resultant mechanical properties of the tested parts were measured. Also, the microstructure obtained by applying the vibration has been examined. Based on these experimental results, the effect of mechanical vibration on mechanical properties of the weld was investigated. Moreover, considering the mechanical properties obtained from these experiments, the optimum values of amplitude, frequency, and welding speed were determined.