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991.
In this paper we show a new method for calculating the nucleolus by solving a unique minimization linear program with O(4n) constraints whose coefficients belong to {−1,0,1}. We discuss the need of having all these constraints and empirically prove that they can be reduced to O(kmax2n), where kmax is a positive integer comparable with the number of players. A computational experience shows the applicability of our method over (pseudo)random transferable utility cooperative games with up to 18 players. 相似文献
992.
Andrea Santos Ion Cristbal Jaime Rubio Cristina Carams Melani Luque Marta Sanz-lvarez Sandra Zazo Juan Madoz-Gúrpide Federico Rojo Jesus García-Foncillas 《International journal of molecular sciences》2022,23(20)
The standard clinical management of locally advanced rectal cancer (LARC) patients includes neoadjuvant 5-fluorouracil (5-FU)-based chemoradiotherapy (CRT) followed by mesorectal excision. MicroRNA (miR)-19b expression levels in LARC biopsies obtained from initial colonoscopy have recently been identified as independent predictors of both patient outcome and pathological response to preoperative CRT in this disease. Moreover, it has been discovered that this miR increases its expression in 5-FU resistant colon cancer cells after 5-FU exposure. Despite the fact that these observations suggest a functional role of miR-19b modulating 5-FU response of LARC cells, this issue still remains to be clarified. Here, we show that downregulation of miR-19b enhances the antitumor effects of 5-FU treatment. Moreover, ectopic miR-19b modulation was able to restore sensitivity to 5-FU treatment using an acquired resistant model to this compound. Notably, we also evaluated the potential clinical impact of miR-19b as a predictive marker of disease progression after tumor surgery resection in LARC patients, observing that miR-19b overexpression significantly anticipates patient recurrence in our cohort (p = 0.002). Altogether, our findings demonstrate the functional role of miR-19b in the progressively decreasing sensitivity to 5-FU treatment and its potential usefulness as a therapeutic target to overcome 5-FU resistance, as well as its clinical impact as predictor of tumor progression and relapse. 相似文献
993.
Valentino Le Noci Giancarla Bernardo Giacomo Manenti Gabriele Infante Dariush Khaleghi Hashemian Lucia Minoli Simone Canesi Francesca Bianchi Tiziana Triulzi Stefania Arioli Loris De Cecco Simone Guglielmetti Federico Ambrogi Camilla Recordati Nicoletta Gagliano Elda Tagliabue Michele Sommariva Lucia Sfondrini 《International journal of molecular sciences》2022,23(21)
994.
995.
Laura Caliogna Valentina Bina Alice Maria Brancato Giulia Gastaldi Salvatore Annunziata Mario Mosconi Federico Alberto Grassi Francesco Benazzo Gianluigi Pasta 《International journal of molecular sciences》2022,23(22)
Bone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their action. Specifically, we evaluated the effects of PEMFs both on human adipose mesenchymal stem cells (hASCs) and on primary human osteoblasts (hOBs) by testing gene and protein expression of early bone markers (on hASCs) and the synthesis of late bone-specific proteins (on hOBs) as markers of bone remodeling. Our results indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells. Understanding the role of PEMFs on bone regenerative processes provides important details for their clinical application. 相似文献
996.
Andrea Piccioni Federico Rosa Federica Manca Giulia Pignataro Christian Zanza Gabriele Savioli Marcello Covino Veronica Ojetti Antonio Gasbarrini Francesco Franceschi Marcello Candelli 《International journal of molecular sciences》2022,23(21)
Our digestive system, particularly our intestines, harbors a vast amount of microorganisms, whose genetic makeup is referred to as the microbiome. Clostridium difficile is a spore-forming Gram-positive bacterium, which can cause an infection whose symptoms range from asymptomatic colonization to fearsome complications such as the onset of toxic megacolon. The relationship between gut microbiota and Clostridium difficile infection has been studied from different perspectives. One of the proposed strategies is to be able to specifically identify which types of microbiota alterations are most at risk for the onset of CDI. In this article, we understood once again how crucial the role of the human microbiota is in health and especially how crucial it becomes, in the case of its alteration, for the individual’s disease. Clostridium difficile infection is an emblematic example of how a normal and physiological composition of the human microbiome can play a very important role in immune defense against such a fearsome disease. 相似文献
997.
Antonella Brizzi Samuele Maramai Francesca Aiello Maria Camilla Baratto Federico Corelli Claudia Mugnaini Marco Paolino Francesco Scorzelli Carlo Aldinucci Luciano De Petrocellis Cinzia Signorini Federica Pessina 《International journal of molecular sciences》2022,23(21)
α-Lipoic acid is a sulfur-containing nutrient endowed with pleiotropic actions and a safe biological profile selected to replace the unsaturated alkyl acid of capsaicin with the aim of obtaining lipoic amides potentially active as a TRPV1 ligand and with significant antioxidant properties. Thus, nine compounds were obtained in good yields following a simple synthetic procedure and tested for their functional TRPV1 activity and radical-scavenger activity. The safe biological profile together with the protective effect against hypoxia damage as well as the in vitro antioxidant properties were also evaluated. Although less potent than capsaicin, almost all lipoic amides were found to be TRPV1 agonists and, specifically, compound 4, the lipoic analogue of capsaicin, proved to be the best ligand in terms of efficacy and potency. EPR experiments and in vitro biological assays suggested the potential protective role against oxidative stress of the tested compounds and their safe biological profile. Compounds 4, 5 and 9 significantly ameliorated the mitochondrial membrane potential caused by hypoxia condition and decreased F2-isoprostanes, known markers of oxidative stress. Thus, the experimental results encourage further investigation of the therapeutic potential of these lipoic amides. 相似文献
998.
Elena Pontemezzo Eleonora Foglio Enza Vernucci Alessandra Magenta Marco DAgostino Sara Sileno Elena Astanina Federico Bussolino Laura Pellegrini Antonia Germani Matteo Antonio Russo Federica Limana 《International journal of molecular sciences》2021,22(9)
Recent findings suggest that epithelial to mesenchymal transition (EMT), a key step during heart development, is involved in cardiac tissue repair following myocardial infarction (MI). MicroRNAs (miRNAs) act as key regulators in EMT processes; however, the mechanisms by which miRNAs target epicardial EMT remain largely unknown. Here, by using an in vitro model of epicardial EMT, we investigated the role of miRNAs as regulators of this process and their potential targets. EMT was induced in murine epicardial-mesothelial cells (EMCs) through TGF β1 treatment for 48, 72, and 96 h as indicated by the expression of EMT-related genes by qRT-PCR, WB, and immunofluorescence. Further, enhanced expression of stemness genes was also detected. Among several EMT-related miRNAs, miR-200c-3p expression resulted as the most strongly suppressed. Interestingly, we also found a significant upregulation of Follistatin-related protein 1 (FSTL1), a miR-200c predicted target already identified as a potent cardiogenic factor produced by epicardial cells that promotes regeneration following MI. Dual-luciferase reporter assay demonstrated that miR-200c-3p directly targeted the 3′-untranslated region of FSTL1 in EMCs. Consistently, WB analysis showed that knockdown of miR-200c-3p significantly increased FSTL1 expression, whereas overexpression of miR-200c-3p counteracted TGF β1-mediated FSTL1 upregulation. Importantly, FSTL1 silencing maintained epithelial features in EMCs, despite EMT induction by TGF β1, and attenuated EMT-associated traits, including migration and stemness. In conclusion, epicardial FSTL1, an important cardiogenic factor in its secreted form, induces EMT, stemness, and migration of EMCs in a miR-200c-3p dependent pathway. 相似文献
999.
1000.
Glenn J. Lematta Christopher C. Corral Verica Buchanan Craig J. Johnson Anagha Mudigonda Federico Scholcover Margaret E. Wong Akuadasuo Ezenyilimba Manuel Baeriswyl Jimin Kim Eric Holder Erin K. Chiou Nancy J. Cooke 《人机工程学与制造业中的人性因素》2022,32(1):133-150
This study focuses on methodological adaptations and considerations for remote research on Human–AI–Robot Teaming (HART) amidst the COVID-19 pandemic. Themes and effective remote research methods were explored. Central issues in remote research were identified, such as challenges in attending to participants' experiences, coordinating experimenter teams remotely, and protecting privacy and confidentiality. Instances of experimental design overcoming these challenges were identified in methods for recruitment and onboarding, training, team task scenarios, and measurement. Three case studies are presented in which interactive in-person testbeds for HART were rapidly redesigned to function remotely. Although COVID-19 may have temporarily constrained experimental design, future HART studies may adopt remote research methods to expand the research toolkit. 相似文献