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81.
Sousa F Mandal S Garrovo C Astolfo A Bonifacio A Latawiec D Menk RH Arfelli F Huewel S Legname G Galla HJ Krol S 《Nanoscale》2010,2(12):2826-2834
In the present study, the in vivo distribution of polyelectrolyte multilayer coated gold nanoparticles is shown, starting from the living animal down to cellular level. The coating was designed with functional moieties to serve as a potential nano drug for prion disease. With near infrared time-domain imaging we followed the biodistribution in mice up to 7 days after intravenous injection of the nanoparticles. The peak concentration in the head of mice was detected between 19 and 24 h. The precise particle distribution in the brain was studied ex vivo by X-ray microtomography, confocal laser and fluorescence microscopy. We found that the particles mainly accumulate in the hippocampus, thalamus, hypothalamus, and the cerebral cortex. 相似文献
82.
Rojo-Bezares B Sáenz Y Zarazaga M Torres C Ruiz-Larrea F 《International journal of food microbiology》2007,116(1):32-36
Nisin is a bacteriocin used against food spoilage bacteria. Sulphur dioxide is a potent antioxidant as well as an antimicrobial agent widely used in the wine industry. In this study we describe the effect of these important antibacterial agents on the growth of a collection of 64 lactic acid bacteria (23 Oenococcus, 29 Lactobacillus, 3 Leuconostoc and 9 Pediococcus), 23 acetic acid bacteria and 20 yeast isolates, most of them recovered from wine. Minimal inhibitory concentrations (MIC) and minimal bactericide concentrations of nisin, potassium metabisulphite and ethanol were determined. Nisin MIC(50) values for the tested isolates were as follows: 0.024, 12.5, 200 and > or micro for oenococci, lactobacilli-pediococci-leuconostoc, acetic acid bacteria and yeasts, respectively. Synergistic effects on bacterial growth inhibition were observed, and potassium metabisulphite MIC(50) values decreased from one to three orders of dilution when it was combined with subinhibitory concentrations of nisin in the growth media. This effect was observed in all lactic acid bacteria species of our study. Significant differences in nisin sensitivity were observed between Gram-positive and Gram-negative bacteria, and between Oenococcus oeni and other species of lactic acid bacteria. It is concluded that appropriate combinations of nisin and metabisulphite could control the growth of spoilage bacteria in wine and therefore allow a decrease in the levels of sulphur dioxide currently used by the wine industry. 相似文献
83.
Fernanda Peyronel Joseph Cooney Erzsebet Papp-Szabo Silvana Martini David Pink 《Journal of the American Oil Chemists' Society》2023,100(12):961-974
Three oleogelator molecules (Triacontane (TC), Stearic acid (SA), and Behenyl Lignocerate (BL)) were studied individually, in pairs, or all together to make an oleogel using triolein as the oil. WAXS, SAXS and USAXS were used to elucidate the solid structures from angstroms to a few micrometers. A two-dimensional mapping of atomic positions for each molecule was carried out to understand the crystalline multilayer structures formed. We assumed that the molecules were rigidly extended and that they underwent no significant (hindered) rotations so that the free energy is determined by the Lennard-Jones interactions of closely packed multilayers. TC molecules were predicted to form a tilt angle of , yielding a SAXS line at Å─1, in acceptable agreement with the measured . For SA crystals (predicted) yielding a SAXS line at compared to (observed). No mixed crystals were observed for any pair of molecules or when all three were used. USAXS data showed that SA forms large nanocrystals compared to TC and BL. All three combinations of molecular pairs showed basic scatterers smaller or similar to those of individual molecules. The theory presented here, together with the experimental results, showed why no mixed crystals are formed from two or all three molecules. Data from the USAXS region suggested that, when using all three molecules, a more compact fractal structure was obtained, compared with those if one or two of the molecules were used. 相似文献
84.
85.
Juliana Alvares Duarte Bonini CamposMiriane Lucindo Zucoloto Fernanda Salloume Sampaio BonaféPaula Cristina Jordani João Maroco 《Computers in human behavior》2011,27(5):1875-1883
Internet data collection is becoming increasingly popular in all research fields dealing with human perceptions, behaviors and opinions. Advantages of internet data collection, when compared to the traditional paper-and-pencil format, include reduced costs, automatic database creation, and the absence of researcher-related bias effects, such as availability and complete anonymity. However, the validity and reliability of internet gathered data must be established, in comparison to the usual paper-and-pencil accepted formats, before an inferential analysis can be done. In this study, we compared questionnaire data gathered from the internet with that from the traditional paper-and-pencil in a sample of college students. The questionnaires used were the Maslach Burnout Inventory - Student Survey (MBI-SS), the Oldenburg Burnout Inventory (OBI-SS) and the Copenhagen Burnout Inventory (CBI-SS). Data was gathered through a within-subject cross randomized and counterbalanced design, on both internet and paper-and-pencil formats. The results showed no interference in the application order, and a good reliability for both formats. However, concordance between answers was generally higher in the paper-and-pencil format than on the internet. The factorial structure was invariant in the three burnout inventories. Data gathered in this study supports the Internet as a convenient, user-friendly, comfortable and secure data gathering method which does not affect the accepted factorial structures existent in the paper format of the three burnout inventories used. 相似文献
86.
Luciana C. Costa Ailton S. Gomes Fernanda M.B. Coutinho Viviane G. Teixeira 《Reactive and Functional Polymers》2010,70(10):738-746
Chelating resins for mercury adsorption were prepared by grafting polyacrylamide chains onto styrene–divinylbenzene (Sty–DVB) copolymers by applying gamma radiation. Sty–DVB copolymers were synthesized by aqueous suspension polymerization employing different synthesis conditions. The copolymers were characterized by apparent density, surface area, pore size distribution and swelling capacity. The copolymers were irradiated using a 60Co-γ source at room temperature in the presence of acrylamide solution in methanol. The grafting reaction was evaluated with the aid of elemental analysis, FTIR and thermogravimetric analysis (TGA). Hg(II) uptake measurements were carried out in batch experiments. The results showed that these resins can be successfully used for Hg(II) adsorption at ppm levels. The porosity degree of the copolymers influences the grafting yield as well as the Hg(II) complexation capacity of the chelating resins. 相似文献
87.
We present our work on novel digitizing techniques to create virtual exhibitions in the context of the Gold Museum in Bogotá, Colombia, a world renown space for precious pre-Colombian gold artifacts. In order to solve issues related to high specular reflection in gold artifacts, we developed a multi-spectral approach that solves some of the shortfalls of many commercial scanners. We also integrated commercial haptic devices into a new virtual installation that allows visitors to touch, hear, and see virtual approximations of the real objects. As an evaluation methodology, we compare the results of a scanner with normal light with our approach, and we also present results from user studies on our virtual installation. 相似文献
88.
89.
The costs to protect a commercial microprocessor against soft errors are discussed in this work. Based on hardware and time redundancies, a protection scheme was designed at RT level to mitigate transient faults on combinational and memory circuits. A fault-tolerant IC version of a mass-produced 8-bit microprocessor is protected by the scheme. Design issues and results in area, performance and power are presented comparing the robust microprocessor with its non-protected version. The costs by flip-flop are also discussed permitting to estimate the overheads in area for any architecture. Furthermore, the RT-level protection scheme is compared with an electrical-level scheme based on a non-standard gate. 相似文献
90.
Soares Carlos R.J.; Gomide Fernanda I.C.; Ueda Eric K.M.; Bartolini Paolo 《Protein engineering, design & selection : PEDS》2003,16(12):1131-1138
The influence of different factors acting on Escherichia coliperiplasmic expression of recombinant human growth hormone (hGH)in shake flask cultures has been investigated. Bacterial vectorscontaining the phage 相似文献