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81.
Gabriel Alves Bonaf Jssica Silva dos Santos Jussara Vaz Ziegler Fernando Augusto Lima Marson Thalita Rocha Manoela Marques Ortega 《International journal of molecular sciences》2022,23(13)
Glycyrrhizic acid (GA), a natural compound isolated from licorice (Glycyrrhiza glabra), has exhibited anti-inflammatory and anti-tumor effects in vitro. Dipotassium glycyrrhizinate (DPG), a dipotassium salt of GA, also has shown an anti-tumor effect on glioblastoma cell lines, U87MG and T98G. The study investigated the DPG effects in the melanoma cell line (SK-MEL-28). MTT assay demonstrated that the viability of the cells was significantly decreased in a time- and dose-dependent manner after DPG (IC50 = 36 mM; 24 h). DNA fragmentation suggested that DPG (IC50) induced cellular apoptosis, which was confirmed by a significant number of TUNEL-positive cells (p-value = 0.048) and by PARP-1 [0.55 vs. 1.02 arbitrary units (AUs), p-value = 0.001], BAX (1.91 vs. 1.05 AUs, p-value = 0.09), and BCL-2 (0.51 vs. 1.07 AUs, p-value = 0.0018) mRNA compared to control cells. The proliferation and wound-healing assays showed an anti-proliferative effect on DPG-IC50-treated cells, also indicating an inhibitory effect on cell migration (p-values < 0.001). Moreover, it was observed that DPG promoted a 100% reduction in melanospheres formation (p-value = 0.008). Our previous microRNAs (miRs) global analysis has revealed that DPG might increase miR-4443 and miR-3620 expression levels. Thus, qPCR showed that after DPG treatment, SK-MEL-28 cells presented significantly high miR-4443 (1.77 vs. 1.04 AUs, p-value = 0.02) and miR-3620 (2.30 vs. 1.00 AUs, p-value = 0.01) expression compared to control cells, which are predicted to target the NF-kB, CD209 and TNC genes, respectively. Both genes are responsible for cell attachment and migration, and qPCR revealed significantly decreased CD209 (1.01 vs. 0.54 AUs, p-value = 0.018) and TNC (1.00 vs. 0.31 AUs, p-value = 2.38 × 10−6) mRNA expression levels after DPG compared to untreated cells. Furthermore, the migration of SK-MEL-28 cells stimulated by 12-O-tetradecanoylphorbol-13-acetate (TPA) was attenuated by adding DPG by wound-healing assay (48 h: p-value = 0.004; 72 h: p-value = 7.0 × 10−4). In addition, the MMP-9 expression level was inhibited by DPG in melanoma cells stimulated by TPA and compared to TPA-treated cells (3.56 vs. 0.99 AUs, p-value = 0.0016) after 24 h of treatment. Our results suggested that DPG has an apoptotic, anti-proliferative, and anti-migratory effect on SK-MEL-28 cells. DPG was also able to inhibit cancer stem-like cells that may cause cerebral tumor formation. 相似文献
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83.
W. A. C. Fernando 《Multimedia Tools and Applications》2006,28(3):301-320
This paper addresses an important area in video processing, namely compressed domain processing. For video indexing, video
scene transition detection is an essential step to segment the video. Current techniques for scene change detection tend to
suffer from a major limitation as most of them cannot identify scene transitions in the compressed domain. Since most video
is expected to be stored in the compressed domain, scene transition detection in this domain is highly desirable. In this
paper an algorithm for video scene change detection is proposed to overcome this limitation. In this scheme, properties of
the B-frames are used as it is capable of measuring the correlation between two adjacent reference frames. The results show
that this scheme performs better than schemes based on P-frames. Proposed scheme can be directly applied with compressed data
with minimum decompression and hence it is computationally efficient and makes real time implementations possible. Results
show that video scene transitions can be identified satisfactorily with the proposed scheme. 相似文献
84.
Celso M. Ogawa Kurt Faltin Fernando A. Maeda Cristina L. F. Ortolani Renata O. Guar Cristiane A. B. Cardoso Andr L. F. Costa 《Microscopy research and technique》2020,83(8):928-936
This study was undertaken to assess in vivo the corrosion in two commercial nickel–titanium (NiTi) orthodontic archwires removed from the oral cavity of patients using fluoride mouthwashes. Five volunteers took part in this study on the corrosion behavior of two brands of NiTi archwires (3M and AO (brand of archwire)) during use of two mouthwashes with neutral sodium fluoride 1.1%, one with acidulated fluoride 1.1%, and one with placebo and a control group. Each patient used one mouthwash in three different periods of time for 1 min a day for 30 days. The archwires were assessed with scanning electron microscopy and atomic force microscopy for qualitative and quantitative analysis. The values obtained with atomic force microscopy (AFM) were submitted to normality test, two‐way analysis of variance, and Tukey's test at a significance level of 5%. The AFM images showed a gradual qualitative increase in the roughness of both types of wire between the treatments: control < placebo < neutral fluoride < acidulated fluoride. The arithmetic average of the roughness and root mean square of the roughness were similar. As for 3M archwires, only the acidulated fluoride group differed statistically from the others. As for AO archwires, the control and placebo groups did not differ from each other, but differed from the other fluoride treatments. The group using neutral fluoride also differed significantly from the acidulated fluoride group. 3M archwires were not affected by daily oral challenges. AO archwires were not affected by daily oral challenges either; their association with fluoride, either neutral or acidulated, increased their roughness. 相似文献
85.
Antônio Alisson Alencar Freitas Fernando Lessa Tofoli Edilson Mineiro Sá Junior Sergio Daher Fernando Luiz Marcelo Antunes 《International Journal of Electronics》2016,103(5):898-912
A high voltage step-up nonisolated DC–DC converter based on coupled inductors suitable to photovoltaic (PV) systems applications is proposed in this paper. Considering that numerous approaches exist to extend the voltage conversion ratio of DC–DC converters that do not use transformers, a detailed comparison is also presented among the proposed converter and other popular topologies such as the conventional boost converter and the quadratic boost converter. The qualitative analysis of the coupled-inductor-based topology is developed so that a design procedure can be obtained, from which an experimental prototype is implemented to validate the theoretical assumptions. 相似文献
86.
Matilde Ortiz-Gonzalez Ignacio Prez-Victoria Inmaculada Ramirez-Macias Nuria de Pedro Angel Linde-Rodriguez Víctor Gonzlez-Menndez Victoria Sanchez-Martin Jesús Martín Ana Soriano-Lerma Olga Genilloud Virginia Perez-Carrasco Francisca Vicente Jos Maceira Carlos A. Rodrígues-Poveda Jos María Navarro-Marí Fernando Reyes Miguel Soriano Jose A. Garcia-Salcedo 《International journal of molecular sciences》2022,23(11)
87.
Marina Paiva Abuafy Regina Celia Galvao Frem Giulia Polinario Fernando Rogerio Pavan Heng Zhao Angelika Mielcarek Cedric Boissiere Christian Serre Leila Aparecida Chiavacci 《International journal of molecular sciences》2022,23(14)
Nanoparticles of metal–organic frameworks (MOF NPs) are crystalline hybrid micro- or mesoporous nanomaterials that show great promise in biomedicine due to their significant drug loading ability and controlled release. Herein, we develop porous capsules from aggregate of nanoparticles of the iron carboxylate MIL-100(Fe) through a low-temperature spray-drying route. This enables the concomitant one-pot encapsulation of high loading of an antitumor drug, methotrexate, within the pores of the MOF NPs, and the collagenase enzyme (COL), inside the inter-particular mesoporous cavities, upon the formation of the capsule, enhancing tumor treatment. This association provides better control of the release of the active moieties, MTX and collagenase, in simulated body fluid conditions in comparison with the bare MOF NPs. In addition, the loaded MIL-100 capsules present, against the A-375 cancer cell line, selective toxicity nine times higher than for the normal HaCaT cells, suggesting that MTX@COL@MIL-100 capsules may have potential application in the selective treatment of cancer cells. We highlight that an appropriate level of collagenase activity remained after encapsulation using the spray dryer equipment. Therefore, this work describes a novel application of MOF-based capsules as a dual drug delivery system for cancer treatment. 相似文献
88.
Soroosh Sharifi‐Asl Fernando A. Soto Tara Foroozan Mohammad Asadi Yifei Yuan Ramasubramonian Deivanayagam Ramin Rojaee Boao Song Xuanxuan Bi Khalil Amine Jun Lu Amin Salehi‐khojin Perla B. Balbuena Reza Shahbazian‐Yassar 《Advanced functional materials》2019,29(23)
LiCoO2 is a prime example of widely used cathodes that suffer from the structural/thermal instability issues that lead to the release of their lattice oxygen under nonequilibrium conditions and safety concerns in Li‐ion batteries. Here, it is shown that an atomically thin layer of reduced graphene oxide can suppress oxygen release from LixCoO2 particles and improve their structural stability. Electrochemical cycling, differential electrochemical mass spectroscopy, differential scanning calorimetry, and in situ heating transmission electron microscopy are performed to characterize the effectiveness of the graphene‐coating on the abusive tolerance of LixCoO2. Electrochemical cycling mass spectroscopy results suggest that oxygen release is hindered at high cutoff voltage cycling when the cathode is coated with reduced graphene oxide. Thermal analysis, in situ heating transmission electron microscopy, and electron energy loss spectroscopy results show that the reduction of Co species from the graphene‐coated samples is delayed when compared with bare cathodes. Finally, density functional theory and ab initio molecular dynamics calculations show that the rGO layers could suppress O2 formation more effectively due to the strong C? Ocathode bond formation at the interface of rGO/LCO where low coordination oxygens exist. This investigation uncovers a reliable approach for hindering the oxygen release reaction and improving the thermal stability of battery cathodes. 相似文献
89.
90.