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排序方式: 共有835条查询结果,搜索用时 46 毫秒
21.
The focus of this article is to review recent techniques in proteomic analysis of ocular fluids. These fluids include tears, aqueous humor, and vitreous, they will also be compared to serum analysis. Furthermore, we attempt to summarize some disease correlated biomarkers in ocular fluids that were discovered through different proteomic techniques in eye diseases like dry eye, glaucoma, age-related macular degeneration, uveitis, or diabetic retinopathy. This review is trying to point out the importance of these biomarkers for clinical applications. 相似文献
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23.
W Beischer L Keller M Maas E Scherfer EF Pfeiffer 《Canadian Metallurgical Quarterly》1976,54(15):709-715
Synthetic human C-peptide bearing a Tyrosine group at its amino end is labelled with 125iodine using chloramin T or hydrogen peroxide and lactoperoxidase. The results are compared applying both methods. Antiserum to synthetic human C-peptide (without Tyrosine) which was partially compared to rabbit albumin, is raised in guinea pigs and goats. Goats show to be superior to guinea pigs concerning antibody production. The so-called "hook effect" phenomenon is observed in setting up the standard curves for the radioimmunoassay. Monotonically decreasing standard curves are obtained on dilution of antiserum with a high antibody titer which was produced by repeated immunization in goats. Free C-peptide and C-peptide bound to antiserum are separated with the anxion exchange resin Amberlite. Using this separation technique we excluded unspecific binding of labelled C-peptide to protein fractions in serum of diabetics. The sensitivity of our radioimmunoassay is approx. 0.3 ng C-peptide/ml serum. Intra- and interassay variability are below 10%. Human proinsulin is the only substance found to crossreact with the antiserum. 相似文献
24.
This paper describes a number of significant modeling considerations for SiGe heterojunction bipolar transistor power amplifiers operating at millimeter-wave frequencies. Small- and large-signal model-to-hardware correlation is presented for single transistor amplifiers, as well as for a combined dual-stage amplifier up to 65 GHz. The relevant parasitic effects are described along with the proposed modeling approach for each of them. The limits of the standard Vertical Bipolar Inter-Company device model at high-injection and their effect on the prediction of the achievable large-signal compression and power-added efficiency are also discussed. 相似文献
25.
Zwick T. Chandrasekhar A. Baks C.W. Pfeiffer U.R. Brebels S. Gaucher B.P. 《Microwave Theory and Techniques》2006,54(3):1001-1010
The focus of this paper is the determination of the complex permittivity of chip packaging materials at millimeter-wave frequencies. After a broad overview of existing measurement techniques, three methods will be presented that have been established for the dielectric property determination of substrate, as well as mold materials (encapsulants, under-fill, etc.) in the millimeter-wave frequency range. First, the open resonator used here will be briefly described. It allows accurate determination of the dielectric constant and loss of thin sheet substrate materials from below 20 GHz to above 100 GHz. Second, a filled waveguide method is explained in detail. The setup used here can determine the complex dielectric properties of mold materials from 70 to 100 GHz. Third, the method based on covered transmission lines will be described in detail. The used lines allow measurements from below 40 GHz to approximately 90 GHz. Verification of all three methods will be provided by inter-comparison and comparison to values from the literature. Additionally, results for several typical substrate and mold materials that are available for millimeter-wave packaging will be shown and discussed. 相似文献
26.
Patricia R. Pfeiffer Angela R. Bielefeldt Tissa Illangasekare Bruce Henry 《Canadian Metallurgical Quarterly》2005,131(10):1447-1452
Laboratory experiments were conducted to investigate the abiotic interactions of soybean oil (SoyOil) and chlorinated ethene (CE) nonaqueous phase liquids (NAPLs). The mixed NAPL density and interfacial tension behaved ideally, as predicted by the volume ratio. The mixed NAPL viscosity increased exponentially from that of the pure CE to that of pure SoyOil as the volume fraction increased. The measured contact angle was highly variable and was unpredictable as a function of the volume composition of the mixed NAPL. The physical property effects indicate that the mobilization of residual CE NAPLs because of SoyOil injection is unlikely. Equilibrium dissolution of CEs from the NAPL mixtures behaved linearly as a function of the mole fraction. Dissolved SoyOil in simulated groundwater enhanced the dissolution of trichloroethene (TCE) during flow tests, increasing the effluent TCE concentration from 141?to?202?mg/L. The ready intermingling of the CE dense nonaqueous phase liquids (DNAPLs) and SoyOil indicate that such interactions may be significant at sites where vegetable oil is injected into DNAPL source areas to enhance in situ anaerobic bioremediation. 相似文献
27.
We present a new solver for coupled nonlinear elliptic partial differential equations (PDEs). The solver is based on pseudo-spectral collocation with domain decomposition and can handle one- to three-dimensional problems. It has three distinct features. First, the combined problem of solving the PDE, satisfying the boundary conditions, and matching between different subdomains is cast into one set of equations readily accessible to standard linear and nonlinear solvers. Second, touching as well as overlapping subdomains are supported; both rectangular blocks with Chebyshev basis functions as well as spherical shells with an expansion in spherical harmonics are implemented. Third, the code is very flexible: The domain decomposition as well as the distribution of collocation points in each domain can be chosen at run time, and the solver is easily adaptable to new PDEs. The code has been used to solve the equations of the initial value problem of general relativity and should be useful in many other problems. We compare the new method to finite difference codes and find it superior in both runtime and accuracy, at least for the smooth problems considered here. 相似文献
28.
Immigrant homeowners’ function within ethnic boundaries in the housing market may have helped or hindered them during the recent U.S. Great Recession. This research explores this theme through interviews with immigrant and non-immigrant homeowners from four ethnic communities in Los Angeles County and the non-profit organizations that tried to assist them. Immigrant homeowners turned to co-ethnics for advice and support and formed multigenerational households as a strategy to achieve and sustain homeownership. Language and cultural barriers primed them for risky loans and thwarted their pursuit of refinance and modification when they struggled to make mortgage payments. These findings conform to existing evidence of ethnic segmentation in the housing market and imply that analyses of home buying and homeownership in areas with significant immigrant populations should factor in the role of ethnicity. 相似文献
29.
Production planning of final assembly systems is a challenging task, as the often fluctuating order volumes require flexible solutions. Besides, the calculated plans need to be robust against the process-level disturbances and stochastic nature of some parameters like manual processing times or machine availability. In the paper, a simulation-based optimisation method is proposed that utilises lower level shop floor data to calculate robust production plans for final assembly lines of a flexible, multi-stage production system. In order to minimise the idle times when executing the plans, the capacity control that specifies the proper operator–task assignments is also determined. The analysed multi-stage system is operated with a pull strategy, which means that the production at the final assembly lines generates demands for the preceding stages providing the assembled components. In order to guarantee the feasibility of the plans calculated for the final assembly lines, a decomposition approach is proposed to optimise the production plan of preceding stages. By this way, the robust production can be ensured resulting in reduced losses and overall production costs even though the system is exposed to changes and disturbances. 相似文献
30.
Vanessa Reusche Prof. Dr. Franziska Thomas 《Chembiochem : a European journal of chemical biology》2021,22(10):1779-1783
A two-step synthesis for methionine-containing hydrophobic and/or aggregation-prone peptides is presented that takes advantage of the reversibility of methionine oxidation. The use of polar methionine sulfoxide as a building block in solid-phase peptide synthesis improves the synthesis quality and yields the crude peptide, with significantly improved solubility compared to the reduced species. This facilitates the otherwise often laborious peptide purification by high-performance liquid chromatography. The subsequent reduction proceeds quantitatively. This approach has been optimised with the methionine-rich Tar-DNA-binding protein 43 (307–347), but is also more generally applicable, as demonstrated by the syntheses of human calcitonin and two aggregation-prone peptides from the human prion protein. 相似文献