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931.
932.
C Sergi K Jundt S Seipp T Goeser L Theilmann G Otto HF Otto WJ Hofmann 《Canadian Metallurgical Quarterly》1998,29(6):861-871
BACKGROUND/AIMS: The aim of the study was to assess the impact factor of HCV and HGV in fulminant hepatic failure. METHODS: The 5'-untranslated regions of HCV RNA and HGV RNA and a segment of the core antigen sequence of HBV were amplified after extracting the nucleic acids from snap-frozen tissue aliquots from explanted livers of 26 consecutive patients undergoing orthotopic liver transplantation for fulminant hepatic failure preoperatively diagnosed as either autoimmune (n=2), HAV/HBV (n=8), toxic (n=4) or aetiologically unknown (n=12). RESULTS: HCV RNA was detected in five of 26 (19.2%) livers with fulminant hepatic failure. All five HCV RNA-positive livers belonged to the group of non-toxic, non-autoimmune liver failure (n=20), three of them were found in the group of liver failure with unknown aetiology (n=12) and two in the group of HBV-associated liver failure (n=7), making an HCV incidence of 25%, 25% and 28.6%, in the different groups, respectively. HGV RNA was detected in 10 of 17 (58.8%) explants and in all four groups of fulminant hepatic failure as defined preoperatively. HBV DNA was identified in six livers of 26 patients (23.1%) with fulminant hepatic failure. Neither HCV RNA nor HBV DNA was detected in the livers of patients with toxic or autoimmune fulminant hepatic failure. CONCLUSIONS: These results indicate that HBV and HCV, but not HGV, play an aetiologic role in fulminant hepatic failure. HCV-positive cases were concentrated either in the group of otherwise unexplained fulminant hepatic failure or in the group of HBV fulminant hepatic failure. HGV-positive cases, on the other hand, were found within all four preoperatively defined groups, indicating a role as cofactor rather than as single aetiologic agent. 相似文献
933.
Determination of cytochrome P450 2E1 activity was carried out via hydroxylation of the synthetic substrate p-nitrophenol to p-nitrocatechol. Crude microsomal preparation isolated from rat liver served as source for cytochrome P450 2E1. Under assay conditions guaranteeing a linear course of the reaction the cytochrome P450 2E1 was stimulated in the presence of a 10(-6) dilution of As2O3 corresponding to 0.915 microM final concentration compared with control. All other concentrations of As2O3 used inhibited the enzyme activity more or less drastically. Furthermore, we used this enzyme system to study the influence of arsenicum album (As2O3) and potassium cyanatum (KCN) in homeopathically prepared (i.e., by consecutive 1:10 steps) and conventional dilutions. We found significant differences between the effects caused by homeopathic potencies (D) and equally concentrated dilutions on catalytic activity of cytochrome P450 2E1. Such differing effects were observed in the case of arsenicum album (As2O3) between D4/10(-4) and D6/10(-6) and in the case of potassium cyanatum (KCN) between D6/10(-6) and D12/10(-12). When we used glutathione-S-transferases and uricase we also found different effects mediated by potencies and conventional dilutions. The results obtained suggest that these three enzyme systems are appropriate detection systems to hunt out differing effects of differently prepared dilutions of specific test substances. 相似文献
934.
The a.c. conductivity of the proteins lysozyme and subtilisin, both in their active and inactive or inhibited forms were determined theoretically. For this, quantum mechanical calculations and a random walk theory were used. The conclusions are that these enzymatic proteins can easily transport electrons which is most probably important in their role as catalysators of biochemical reactions. The electron transport is sensitive to three-dimensional changes of the active sites of these enzymes which have more flexibility than the whole molecule. Therefore, the flexibility of the active sites of enzyme catalysts is not only important for the conformational fitting with the reactants, but also strongly influences the transport in biochemical reactions. 相似文献
935.
W Eggert-Kruse G Rohr H Kerbel B Schwalbach T Demirakca K Klinga W Tilgen B Runnebaum 《Canadian Metallurgical Quarterly》1996,11(4):784-789
To determine the clinical usefulness of Acridine Orange (AO) staining of spermatozoa as a screening test for the evaluation of semen quality during basic infertility investigation, semen smears from 103 randomly chosen males of subfertile couples were examined. The median duration of infertility was 4.5 years (range 1-15) and the median age was 33 years (range 21-43). The outcome of AO staining ranged from 5 to 81%, with a median of 24%, green fluorescent spermatozoa. Results were not significantly related to the parameters of semen analysis (sperm count, motility, standard morphology, viability, pH and volume, as well as fructose concentration and number of found cells) or to local sperm antibody testing and semen cultures. Fluorescence after AO staining was also not related to sperm functional capacity (evaluated using sperm-mucus interaction tests in vitro and in vivo), or the medical history of the patient. No significant differences in the AO test outcome were seen in patients with explained and unexplained infertility, or with regard to subsequent fertility [with a median value of 21% (range 5-46) green fluorescence in the fertile group, compared with a median value of 28% (range 9-81) green fluorescence in the other men]. The results of this prospective study indicate that under the usual conditions of conception, the AO test is not clinically useful as a screening procedure to determine semen quality during basic infertility investigation. 相似文献
936.
V. E. Gul O. A. Sdobnikova A. L. Peshehonova L. G. Samoilova O. A. Khanchech A. I. Suvorova 《Polymer Engineering and Science》1993,33(22):1489-1493
Reported is an attempt to attain orientation of macromolecules during extrusion of films of cellulose derivatives that led to the liquid-crystalline state of a polymer and resulted in enhanced film strength. 相似文献
937.
V. F. Shevchenko A. A. Petrov V. G. Petrov 《Journal of Infrared, Millimeter and Terahertz Waves》1993,14(9):1755-1768
A basic principle of pulse radar reflectometry is considered in this paper. A numerical analysis is performed in order to study errors appearing due to the expansion of a microwave pulse reflected from the plasma. A block diagram of the basic pulse radar scheme and its overall performance is presented. The first experimental results obtained on T11-M tokamak are discussed. An improved pulse radar scheme with cross-detection is considered. The results of bench test experiments and future applications of this scheme are discussed. 相似文献
938.
A self-routing connection network is a switching device where the routing of each switch can be determined in terms of the destination addresses of its inputs alone, i.e. independent of the routing information regarding the other switches in the network. One family of connection networks that were considered in the literature for self-routing are Clos networks. Earlier studies indicate that some Clos networks can be self-routed for certain permutations. In this paper, it is proved that the only category of Clos networks that can be self-routed for all permutations are those with at most two switches in their outer stages 相似文献
939.
Results from applying the model on a sample of contractors, the majority of whom were international and operating in Egypt, reinforces the credibility of the developed methodology, claim the authors. 相似文献
940.
In a survey of the practical wind energy resource present in the Tayside Region of Scotland it was estimated that over 1500 km2 of land is suitable for wind energy development in the Region after consideration of a range of physical, technical and institutional factors. Wind speed data for this survey was obtained from the Energy Technology Support Unit (ETSU) UK Wind Speed Data Package. To verify the wind speeds obtained from the ETSU package a representative sample of sites in and around the identified areas of potential in Tayside were modelled for mean annual wind speed using the Wind Atlas Analysis and Application Program (WAsP). The wind speeds for the sites obtained from the WAsP analysis were compared with those obtained from the ETSU UK Wind Speed Data Package and conclusions drawn as to the reliability of the Tayside wind energy survey and the general applicability of the ETSU package for broad wind energy resource assessment. 相似文献