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51.
Identification of a gene that causes primary open angle glaucoma 总被引:3,自引:0,他引:3
EM Stone JH Fingert WL Alward TD Nguyen JR Polansky SL Sunden D Nishimura AF Clark A Nystuen BE Nichols DA Mackey R Ritch JW Kalenak ER Craven VC Sheffield 《Canadian Metallurgical Quarterly》1997,275(5300):668-670
Glaucoma is a major cause of blindness and is characterized by progressive degeneration of the optic nerve and is usually associated with elevated intraocular pressure. Analyses of sequence tagged site (STS) content and haplotype sharing between families affected with chromosome 1q-linked open angle glaucoma (GLC1A) were used to prioritize candidate genes for mutation screening. A gene encoding a trabecular meshwork protein (TIGR) mapped to the narrowest disease interval by STS content and radiation hybrid mapping. Thirteen glaucoma patients were found to have one of three mutations in this gene (3.9 percent of the population studied). One of these mutations was also found in a control individual (0.2 percent). Identification of these mutations will aid in early diagnosis, which is essential for optimal application of existing therapies. 相似文献
52.
TD Foley 《Canadian Metallurgical Quarterly》1997,235(2):374-376
The effects of 1 microM concentrations of arachidonic acid hydroperoxide (HPETES) products of 5-, 12- and 15-lipoxygenase on Na+, K(+)-ATPase activity were investigated in synaptosomal membrane preparations from rat cerebral cortex. 5-HPETE inhibited Na+, K(+)-ATPase activity by up to 67 %. In contrast, 12-HPETE and 15-HPETE did not inhibit Na+, K(+)-ATPase activity. In addition, neither 5-HETE or LTA4 inhibited Na+, K(+)-ATPase activity. Dose-response studies indicated that 5-HPETE was a potent (IC25 = 10(-8) M) inhibitor of Na+, K(+)-ATPase activity. These findings indicate that 5-HPETE inhibits Na+, K(+)-ATPase activity by a mechanism that is dependent on the hydroperoxide position and independent of further metabolism by 5-lipoxygenase. It is proposed that 5-HPETE production by 5-lipoxygenase and subsequent inhibition of neuronal Na+, K(+)-ATPase activity may be a mechansim for modulating synaptic transmission. 相似文献
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Döne Demirgöz Rodrigo Navarro Mónica Pérez Helmut Reinecke Alberto Gallardo 《应用聚合物科学杂志》2010,115(2):896-900
2‐Hydroxyethylmethacrylate (HEMA) and acrylamide (AA) have been copolymerized via free radical mechanism, in the presence of 5 mol % of four different crosslinker systems, the symmetric ethylenglycoldimethacrylate (EGDMA), bisacrylamide (BIS), a mixture of EGDMA and BIS, and the asymmetric acrylamideethylenmethacrylate (METAA). The polymerizations have been monitored with a rheometer, exhibiting the gel obtained with the asymmetric METAA, an elastic modulus that is dramatically increased compared with those of the gels prepared with the other three crosslinker systems. A kinetic analysis using the terminal model has been used to build probabilistic surfaces that give information about how the crosslinker is incorporated into the network. This analysis shows a high dissimilarity between the reactions using the asymmetric and the mixture of symmetric crosslinkers, what has been correlated to the difference in modulus. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010 相似文献
54.
A method to prepare water sensitive composites employing crosslinked poly(vinyl amine) (PVAm) and Cu(II) ions as reticulation agent is disclosed in this work. This article presents in detail, the reticulation reaction and its pH dependence, as well as its electrical, rheological, and thermal properties of the PVAm‐Cu(II) composites obtained. The complex bonds formed between the Cu(II) ions and the amine groups of PVAm produce an interesting network of crosslinked structures that generate a microporous morphology when the material is extruded. This characteristic favors fast absorption of water when it is wetted, and a concomitant decrease in its apparent resistivity in a very short period of time. Furthermore, they present an excellent thermal stability and suitable processability. These characteristics make them interesting candidates to design ultra fast water sensors. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010 相似文献
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Pregnant Sprague-Dawley rats were administered primidone (PRM) by oral gavage on gestation days 8-17 in doses of 0.40, and 80 mg/kg. Although these doses of PRM did not produce significant differences in litter size, birth weight, mortality, date of attainment of developmental landmarks or measures of preweaning reflex and motor development, there were a number of significant differences that developed as the animals approached and entered adulthood. When tested as adults, the 80 mg/kg male rats showed a deficit in the performance of an eight-arm radial maze task. These same animals showed a significant reduction in open field activity when tested as adults. In addition, both male and female PRM-treated animals showed reduced body weights at different periods corresponding to onset of sexual maturation during development. These findings are consistent with the larger body of literature reporting on the neurobehavioral teratology of phenobarbital, including its ability to produce lesions in the hippocampus and endocrine dysfunction resulting in reproductive deficits. These results suggest that PRM produces its adverse effects as a result of its metabolism to phenobarbital, which in turn affects the limbic system. 相似文献
58.
C. Pi?eiro J. Barros-Velázquez Carmen G. Sotelo José M. Gallardo 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1999,11(12):342-348
The water-soluble proteins of nine flat fish species of high commercial value, belonging to the Pleuronectidae, Scophtalmidae, and Soleidae families, were analyzed by two-dimensional (2D) electrophoresis, this being carried out by nondenaturing isoelectric focusing (IEF) in the 3.5–9.5 pH range and gradient SDS-PAGE in the 12–14% range. Most of the major proteins fell in the 3.5–6.9 pH range. From these, the most specific proteins were in the acidic fraction (pI<5.2; MW<16 kDa). Species-specific 2D protein patterns were observed in all the nine species, and more than 25 proteins could be individualized. The combination of nondenaturing IEF and gradient SDS-PAGE, both in the absence of urea, allowed detailed characterization of both the isoelectric points and molecular weights of the major water-soluble proteins and proved to be a valuable tool for the differential characterization of the flat fish species studied. 相似文献
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