Importance of platelets in increased vascular permeability evoked by experimental haemarthrosis in synovium of the rat

Increased vascular permeability of synovium induced by experimental haemarthrosis was studied in the stifle joint of the rat. Abnormal permeability was detected by injecting animals intravenously with colloidal carbon and examining the synovial vessels for intramural deposits of carbon. Both fresh and heparinized whole blood injected into the joint induced a marked permeability response of synovial venules which persisted for 18 hours. Platelets suspended in heparinized Tyrode solution induced a similar response, but one lasting only 12 hours. The permeability effects of suspensions of leucocytes and erythrocytes on the synovial vasculature were relatively mild and were maximal 5 and 12 h respectively after intra-articular injection. Heparinized platelet-free plasma had no significant permeability effects. These results indicate that platelets may play an important role in the pathogenesis of synovial inflammation in haemarthrosis.     

Performance of Offset Quadriphase Spread-Spectrum Multiple-Access Communications

New analytical results are obtained on the performance of quadriphase spread-spectrum multiple-access communications. The primary performance parameter considered is the signal-to-noise ratio at the receiver output. This parameter includes the effects of multiple-access interference and channel noise, and it has been shown to give a reliable estimate of the average probability of error.     

Universal versus personal helplessness in depression: Belief in uncontrollability or incompetence?

The learned helplessness model of depression predicts that depressed individuals believe outcomes are more response independent than do nondepressed individuals in a skill situation. The present study assessed whether depressives' cognitive distortions are specific to their belief about their own skilled action or are a result of a general belief in uncontrollability in the world. Changes in expectancies following success and failure in skill and chance tasks were examined in 32 depressed and 32 nondepressed college students who either performed themselves or observed a confederate perform a pair of tasks. In the skill task, depressed Ss showed significantly smaller changes in expectancy than nondepressed Ss when estimating the probability of their own success. In contrast, depressed and nondepressed Ss did not differ when estimating the probability of another person's success on the identical skill task. It is inferred that depressed individuals view themselves as helpless in a skilled situation but do not view the situation itself as uncontrollable. Results are discussed in terms of the reformulated learned helplessness model. (52 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

1.4 Fast volume determination using a differential capacitance manometer

The reliable pressure readings with a capacitance manometer are the basis for a new type of instrument designed for accurate and rapid measurements of capillary volumes. The basic measuring principle is based on expansion of air atmospheric pressure enclosed in a known volume into a pre-evacuated cylinder so that the enclosed volume will cause a reduced pressure in the cyllinder. The same is done with the air in an unknown volume into another identical pre-evacuated cylinder. A differential capacitance manometer is then used to measure the difference in pressure between the two cylinders with a high degree of accuracy. This difference in pressure is shown to be ainear function of the difference in volume between the unknown and the known volume. The accuracy and repeatability of the instrument is better than 0.5%.     

Acid-base and electrolyte effects of shortening steeplechase in a three-day-event

This study was designed to characterise the acid-base and electrolyte effects of shortening the distance required during steeplechase (Phase B) in the face of hot and humid weather conditions during a treadmill-simulated Speed and Endurance test. Eight conditioned Thoroughbred horses underwent 3 randomised permutations of a standardised exercise test on a high speed treadmill. Each test consisted of trotting at 3.7 m/s for 10 min (Phase A); galloping at 11 m/s (Phase B) for 4 (cool laboratory conditions), 3 (hot and humid), or 2 (hot and humid) min; trotting at 3.7 m/s for 30 min (Phase C); and walking at 1.8 m/s for 10 min (Phase X). The treadmill slope was 4% for trotting and galloping and 0% for walking. Cool versus hot and humid conditions were 20 degrees C and 50-60% relative humidity vs. 26-28 degrees C and 80-85% relative humidity, respectively. Pulmonary artery blood samples were obtained at rest prior to exercise (Rest); at the end of Phases A (A10) and B (B2-4); at 10 (C10), 20 (C20) and 30 (C30) min through Phase C; and at 5 min into Phase X (X5). Additional samples for lactate (LA) and glucose (GLC) analysis were obtained 5 min into Phase C (C5) and at the end of Phase X (X10). Samples were analysed for packed cell volume (PCV), haemoglobin (HB), total plasma protein (TP), sodium (Na), potassium (K), chloride (Cl), anion gap (AG), plasma glucose (GLC) and lactate (LA), pH, PCO2, bicarbonate (HCO3) and base excess (BE). Shortening steeplechase distance by 50% under hot and humid conditions (2 min B) resulted in a consistent return to control measurements (4 min B) only for plasma LA. Changes in PCV, HB, TP, K and Cl were related more to the longer galloping distance in the 4 min B trials than to hot vs. cold laboratory conditions. Alternatively, changes in LA, GLC, pH, PCO2 and AG were more related to hot and humid laboratory conditions than they were to galloping distance. These latter variables, when combined with physical measures such as core temperature, bodyweight loss, point of fatigue on Phase C and recovery heart rates may serve as the best monitors of positive responses in future studies of proposed modifications to Phase C, rather than those variables which were more distance than weather-related.     

Contribution of rpoB mutations to development of rifamycin cross-resistance in Mycobacterium tuberculosis

The contributions of 23 insertion, deletion, or missense mutations within an 81-bp fragment of rpoB, the gene encoding the beta-subunit of the DNA-dependent RNA polymerase of Mycobacterium tuberculosis, to the development of resistance to rifamycins (rifampin, rifabutin, rifapentine, and KRM-1648) in 29 rifampin-resistant clinical isolates were defined. Specific mutant rpoB alleles led to the development of cross-resistance to all rifamycins tested, while a subset of mutations were associated with resistance to rifampin and rifapentine but not to KRM-1648 or rifabutin. To further study the impact of specific rpoB mutant alleles on the development of rifamycin resistance, mutations were incorporated into the rpoB gene of M. tuberculosis H37Rv, contained on a mycobacterial shuttle plasmid, by in vitro mutagenesis. Recombinant M. tuberculosis clones containing plasmids with specific mutations in either codon 531 or 526 of rpoB exhibited high-level resistance to all rifamycins tested, whereas clones containing a plasmid with a mutation in codon 516 exhibited high-level resistance to rifampin and rifapentine but were susceptible to both rifabutin and KRM-1648. These results provided additional proof of the association of specific rpoB mutations with the development of rifamycin resistance and corroborate previous reports of the usefulness of rpoB genotyping for predicting rifamycin-resistant phenotypes.