Effects of aging, diet, and sex on plasma glucose, fructosamine, and lipid concentrations in barrier-raised Fischer 344 rats

We studied the relationships of plasma glucose, fructosamine, triglycerides, and cholesterol as a function of age, gender, and diet in barrier-raised Fischer 344 rats aged 5 to 26 months, fed a diet either ad libitum or restricted to 60% of the ad libitum caloric intake. The complex relationships of these plasma levels to age, gender, and diet led to the development of a model with age, diet, and sex as covariates. Overall, fasting plasma glucose concentrations were reduced by approximately 25% in rats on the restricted diet, compared to ad libitum-fed animals. There was a significant age-dependent decline in glucose levels in male animals, whereas in females there was an increase in plasma glucose with aging. Plasma fructosamine levels in calorie-restricted animals, overall, were reduced by 7% compared to levels in animals fed ad libitum. There was a significant positive correlation between plasma glucose and fructosamine levels. Mean plasma triglyceride content was decreased by 50% in calorie-restricted rats compared to ad libitum-fed animals. A significant decrease in triglyceride levels with increasing age was seen in male animals, and an increase with aging in females. There was a significant positive correlation between plasma glucose and triglyceride levels. Plasma cholesterol levels in calorie-restricted animals were reduced by 7% compared to levels in ad libitum-fed animals. An increase of cholesterol concentration with aging was significant in both males and females. Analysis of the data showed that there were significant differences between male and female Fischer 344 rats in the response of plasma glucose and fructosamine to aging and calorie restriction. Changes of plasma triglyceride and cholesterol with aging and dietary calorie restriction were also different in males and females. Studies of the effect of aging on glycemia and blood lipid content should take into account the contributions of animal sex.     

A study of the capacity of soils to induce nodules in Alnus glutinosa (L.) Gaertn. and Myrica gale L., with special reference to the specificity of the endophytes

1. Several ring-substituted derivatives of diphenyleneiodonium catalyse the exchange of Cl- and OH- ions across the inner membrane of rat liver mitochondria. They also inhibit state 3 and state 3u oxidations of glutamate plus malate in the presence of Cl- more than in its absence. Most have activities similar to diphenyleneiodonium, although 2,4-dichlorodiphenyleneiodonium is up to 50 times more active. 2. Diphenyleneiodonium inhibits soluble rat liver NADH dehydrogenase and NADH oxidation by rat liver sub-mitochondrial particles directly; 2,4-dichlorodiphenyleneiodonium is only about twice as inhibitory. 3. Liver mitochondria contain two classes of binding sites for diphenylene[125I]iodonium, namely high-affinity sites with an affinity constant of 3 X 10(5) M-1 (1--2 nmol/mg of protein), and low-affinity sites with an affinity constant of 1.3 X 10(3) M-1 (80 nmol/mg of protein). Both sites occur in hepatocytes with a relative enrichment of the low-affinity site. Nadh dehydrogenase preparations only apparently contain high-affinity binding sites. Only low-affinity sites occur in erythrocytes. 4. 2,4-Dichlorodiphenyleneiodonium competes with diphenylene[125I]iodonium for both low- and high-affinity sites, whereas tri-n-propyltin only competes for the low-affinity sites. 5. The high-affinity sites are apparently associated with NADH dehydrogenase and the low-affinity sites probably represent electrostatic binding of diphenylene[125I]iodonium to phospholipids. The high-affinity site does not appear to be associated with a rate-limiting stage of NADH oxidation.     

Antagonism of croton oil inflammation by topical emu oil in CD-1 mice

Emu oil is derived from the emu (Dromaius novaehollandiae), which originated in Australia, and has been reported to have anti-inflammatory properties. Inflammation was induced in anesthetized CD-1 mice by applying 50 μL of 2% croton oil to the inner surface of the left ear. After 2 h, the area was treated with 5μL of emu, fish, flaxseed, olive, or liquified chicken fat, or left untreated. Animals were euthanized at 6 h postapplication of different oils, and earplugs (FP) and plasma samples were collected. Inflammation was evaluated by change in earlobe thickness, increase in weight of EP tissue (compared to the untreated ear), and induction in cytokines interleukin (IL)-1α and tumor necrosis factor-α (TNF-α) in EP homogenates. Al-though reductions relative to control (croton oil) were noted for all treatments, auricular thickness and EP weights were, significantly reduced (−72 and −71%, respectively) only in the emu oil-treated group. IL-1α levels in homogenates of auricular tissue were significantly reduced in the fish oil (−57%) and emu oil (−70%) groups relative to the control group. The cytokine TNF-α from auricular homogenates was significantly reduced in the olive oil (−52%) and emu oil (−60%) treatment groups relative to the control group. Plasma cytokine levels were not changed by croton oil treatment. Although auricular thickness and weight were significantly correlated with each other (r=0.750, P<0.003), auricular thickness but not weight was significantly correlated with cytokine IL-1α (r=0.750, P<0.006) and TNF-α (r=0.690, P<0.02). These studies indicate that topical emu oil has anti-inflammatory properties in the CD-1 mouse that are associated with decreased auricular thickness and weight, and with the cytokines IL-1α and TNF-α.     

Optical and microstructural properties of p-type SrCu2O2: First principles modeling and experimental studies

In this paper, we report first principles calculations and experimental studies of the optical and microstructural properties of both bulk and thin films of SrCu₂O₂. Polycrystalline SrCu₂O₂ films were grown by a conventional Pulsed Laser Deposition method in a flowing oxygen environment on corning glass 7059 and silicon substrates. Several characterization techniques, including X-ray diffraction (XRD), Fourier Transform IR (FTIR), Raman, spectroscopic ellipsometry, reflectance/transmission spectrophotometry and Atomic Force Microscopy have been used for the investigation of the microstructural and vibrational properties of both bulk and thin films of SrCu₂O₂.XRD shows that bulk SrCu₂O₂ is polycrystalline and assumes the pure tetragonal phase of SrCu₂O₂. The vibrational properties of the tetragonal phase of SrCu₂O₂ have been inferred from Raman and FTIR spectroscopies and for the first time both Raman and IR active modes have been assigned. The bulk polycrystalline SrCu₂O₂ optical band gap determined from spectroscopic ellipsometry was 3.34 ± 0.01 eV. XRD results confirmed that pure non-textured polycrystalline phase SrCu₂O₂ thin films with a smooth surface can be grown by PLD at low temperature (300 °C).     

Shape Selective Chemistries with Modified Mordenite Zeolites

This is a review of work carried at Dow Chemical on the design and characterization of novel mordenite catalysts with a unique micro and meso-porous structure characterized by a variety of methods including, TEM, BET surface area and pore size distribution, and n-decane hydro-conversion. The catalytic performance of these catalysts in shape selective reactions is illustrated with results from n-decane hydro-conversion and applications to alkylation and trans-alkylation reactions of mono and polynuclear aromatic hydrocarbons and other chemistries. Juan M. Garcés and G. John Lee both retired from The Dow Chemical Company.     

Omental transposition flap for gross locally recurrent breast cancer

A randomized double-blind controlled trial was undertaken to study the efficacy of a cow udder ointment versus petroalatum alone. A total of 30 patients participated in the study. Sixteen patients completed the trial with 8 of 9 patients improving in the active group and 6 of 7 patients improving in the placebo group. This difference was not statistically significant. The use of a cow udder ointment for psoriasis cannot be supported, particularly with the potential for side effects.