On the Dynamic Performance of Flax Fiber Composite Beams Manufactured at Different Relative Humidity Levels

ABSTRACT

Development of environment-friendly natural fiber composites has been a recent trend. However, due to the fact that natural fibers permit high level of moisture absorption from the surroundings, it can lead to weak bindings and degradation of composite properties. This paper presents an experimental study on the dynamic performance of flax fiber composite beams manufactured at different relative humidity (RH) levels. Five types of flax fiber-reinforced composite materials were made under different RH values, i.e., dry, 35%, 50%, 70%, and 95% RH, and beam samples were prepared using the composite. Impact hammer testing was conducted to measure the natural frequencies and damping of the beams. It was found that for the first three modes, while the resonant frequencies are very close for most samples, there is a clear drop of frequencies for the composite fabricated at 95% RH. Along with an increase of the RH level, the damping ratios for all the three modes have reported a slight increase, but the variation is not significant.     

Monitoring of the discoloration on γ‐irradiated PE and EVA films to evaluate antioxidant stability

γ‐Irradiated films could provoke unexpected interaction with proteins for instance just after irradiation and not necessarily after 12 months indicating there is no more reactive species. The optical properties of two multilayer films [polyethylene (PE)/ethylene vinyl alcohol (EVOH)/PE and ethylene vinyl acetate (EVA)/EVOH/EVA] after different γ‐irradiation doses is then studied in this work. The investigation on these films, either non‐irradiated or γ‐irradiated (up to 270 kGy), is performed by colorimetry measurement over time (up to 12 months) to assess the generation of new species inside the materials. The color change is directly correlated with absorbed γ‐doses. Over time, the color decreases and goes back to its initial time level. This discoloration evolution could be therefore used as an indication of the completion of the generated species reactions induced by γ‐irradiation. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 46114.     

Nanopatterned UV curable hydrogels for biomedical applications

With an increasing use of emerging patterning technologies such as UV-NIL in biotechnological applications there is at the same time a raising demand for new material for such applications. Here we present a PEG based precursor mixed with a photoinitiator to make it UV sensitive as a new material aimed at biotechnological applications. Using HSQ patterned quartz stamps we observed excellent pattern replication indicating good flow properties of the resist. We were able to obtain imprints with <20 nm residual layer. The PEG based resist has hydrogel properties and it swelling in water was observed by AFM.     

Limitations of codon adaptation index and other coding DNA-based features for prediction of protein expression in Saccharomyces cerevisiae

The relationship between codon usage and protein/mRNA expression in S. cerevisiae has been extensively studied. Recently, protein expression data for the whole yeast genome was published. We investigate which properties of coding DNA sequences can be used to predict expression levels. The new algorithm by Carbone et al. for computing dominating codon bias in a genome is evaluated. It is concluded that it works at least as well as existing methods, and eliminates the need to arbitrarily choose a set of highly expressed genes. Also, the hypothesis that information on codon pair frequencies can be used to predict expression is investigated. Our conclusion is that codon pairs do not contribute more information than do single codon frequencies. Overall correlation between predicted and actual expression data using properties of coding DNA sequences is around 0.65. Hence, while being a useful source of information, the expression levels predicted by these methods should only be used as a rule of thumb.     

Compact LED based nanoimprinter for UV-NIL

UV-based nanoimprint lithography (UV-NIL) is a cheap and fast way to imprint patterns ranging from nanometres to micrometres. However, commonly used equipment can be expensive and require a clean room infrastructure. Here we present the design and testing of a simple UV-NIL system based on a light emitting diode. The current design permits imprints of 10 × 10 mm² in size using a 25 × 25 mm² master. This printer can be used in a semi-clean environment such as a laminar flow bench. The imprinter was used to imprint photoresists as well as UV sensitised hydrogels. The best results were obtained using SU-8 photoresist with features down to 50 nm in size, only limited by the imprint master. Patterns in SU-8 resist were also transferred into silicon substrates by reactive ion etching demonstrating its full potential as a lithographic tool.     

Heat shock proteins and arthritis--new readers start here

We have characterised G protein and fatty acid regulation of the Na+ conductance in purified apical membrane vesicles prepared from late gestation fetal guinea-pig lung. Addition of 100 microM GTP gamma S or beta gamma-methylene-GTP, irreversible G protein activators, stimulated conductive 22Na+ uptake (ratio of experimental to control 1.35 +/- 0.02 and 1.34 +/- 0.05, respectively). Conversely, the addition of GDP beta S, an irreversible G protein inhibitor, reduced conductive 22Na+ uptake from 1.00 (control) to 0.79 +/- 0.04. A range of saturated (myristic, palmitic, stearic), monounsaturated (elaidic, oleic) and polyunsaturated (linoleic, arachidonic) fatty acids all stimulated conductive 22Na+ uptake, by between 1.18 +/- 0.05 to 1.56 +/- 0.13 over the control. Both arachidonic acid and GTP gamma S-dependent stimulation were abolished in the presence of 10 microM amiloride. The non-metabolisable analogue of arachidonic acid, eicosa-5,8,11,14-tetraynoic acid also stimulated conductive 22Na+ uptake. Furthermore, addition of indomethacin and nordihydroguairetic acid, inhibitors of cyclooxygenase and lipoxygenase pathways of arachidonate metabolism respectively, did not affect the arachidonic acid stimulation suggesting a direct effect of fatty acid upon the Na+ channel Since mepacrine (50 microM), a phospholipase A2 inhibitor, did not affect the GTP gamma S-stimulated conductive 22Na+ uptake, and inhibition of G protein turnover by GDP beta S did not attenuate the arachidonic acid response we conclude that these two regulatory pathways modulate alveolar Na+ transport directly and independently of each other.     

Controlling exit site infections: Does it decrease the incidence of catheter‐related bacteremia in children on chronic hemodialysis?

The aim of this retrospective study was to investigate whether the application of a chlorhexidine-impregnated dressing (Biopatch^®) at the exit site of tunneled-cuffed hemodialysis catheters has any effect on the incidence and etiology of catheter-related bacteremia (CRB). This study was carried out over a 5-year period in a single center, where, in the first 2½ years, the exit sites were cleansed with betadine at every hemodialysis session and then covered with a transparent dressing (pre-Biopatch^® Era). During the next 2½ years, Biopatch^® was applied to the exit site once a week after cleansing with betadine, and then covered with a transparent dressing (Biopatch^® Era). The application of Biopatch^® significantly decreased the incidence of exit site infections (ESI) (P<0.05). However, there was no difference in the incidence of CRBs or their microbiological distribution. The improved ESI rate had no effect on the overall catheter survival time. The antimicrobial sensitivities of the Gram-positive microorganisms were statistically different for the 2 different types of infections (P<0.05). In conclusion, even though Biopatch^® is effective in decreasing the incidence of ESI, it has no effect on the incidence of CRB, the etiology of CRB, or the overall catheter survival time. The distinct difference between the antimicrobial sensitivities of the ESI and CRB suggests that they are not a spectrum of the same pathogenesis. These preliminary data support the intraluminal pathogenesis of CRB, rather than the exit site as a possible entry point for the extraluminal route.     

Mine Water as a Resource: Selective Removal and Recovery of Trace Antimony from Mine-Impacted Water

The objective of this study was to evaluate the feasibility of combined modular processes to selectively remove Sb from mine-impacted waters in an Arctic environment in order to fulfil local environmental criteria for discharged waters. Novel ion exchange, selective extraction and ultrafiltration, electrocoagulation, and dissolved air flotation technologies were investigated, individually or in combination, from the laboratory- to pilot-demonstration scale. Laboratory-scale testing using Fe₂(SO₄)₃ precipitation, ion exchange resin, selective ion extraction and ultrafiltration, and electrocoagulation with or without subsequent dissolved air flotation indicated that any of the methods are potentially applicable to Sb removal from mine water. The observed differences between Sb and As removal efficiency by ion exchange resin illustrated the need for Sb-specific removal and recovery technologies. Techno-economic analyses showed that treatment of mine water using electrocoagulation-dissolved air flotation yields the lowest comparative life-cycle cost of examined technologies. Results demonstrated increased Sb attenuation efficiency using either electrocoagulation-dissolved air flotation or selective extraction and ultrafiltration, even when treating only 50% of the mine-impacted water, compared with conventional Fe₂(SO₄)₃ precipitation from mine water. Additional investigation is necessary to characterize the long-term stability of the mineral phases in Sb-containing solid residues and to inform selection of Sb recovery methods and utilisation or final disposal options for the residual materials.