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61.
Giovanna Rassu Elisabetta Gavini Gianpiera Spada Salvatore Marceddu 《Drug development and industrial pharmacy》2013,39(11):1178-1187
The preparation of ketoprofen spray-dried microspheres can be affected by the long drug recrystallization time. Polymer type and drug–polymer ratio as well as manufacturing parameters affect the preparation. The purpose of this work was to evaluate the possibility to obtain ketoprofen spray-dried microspheres using the Eudragit® RS and RL; the influence of the spray-drying parameters on morphology, dimension, and physical stability of microspheres was studied. Ketoprofen microspheres based on Eudragit® blend can be prepared by spray-drying and the nebulization parameters do not influence significantly particle properties; nevertheless, they can be affected by drying and storage methods. No effect of the container material is found. 相似文献
62.
Giulia Bernardini Maurizio Comanducci Stefania Bambini Giovanni Renzone Andrea Scaloni Giovanna Morelli Mark Achtman Giulio Ratti Annalisa Santucci 《Proteomics. Clinical applications》2009,3(10):1251-1254
We previously described the first reference map for the proteome of one strain of serogroup A Neisseria meningitidis (MenA), a major cause of epidemic meningitis in humans. As a preliminary finding, in that work we noted that 2‐DE protein maps of closely related MenA isolates from different epidemics spreads could be easily compared to detect minor differences and that 2‐DE phenotypes attributable to the well‐known epidemiological marker tbpB agreed with the genoclouds model of MenA epidemiological variation during pandemic waves. We explored here the possibility that an extended comparative study of 2‐DE maps of isolates representative of the nine genoclouds described by Achtman and collaborators could be used to discriminate between strains otherwise undistinguishable. We showed the example of 14 proteins with different 2‐DE spot patterns in different genoclouds that could be considered as putative tracers for alike‐strains discrimination. We introduce the novel concept that comparative proteomics can be useful in identifying new epidemiological markers for N. meningitidis. 相似文献
63.
Maria Silvia Tonti Marco Masseti Giovanna Poli Massimo Paci Pierluigi Magagnini Claudia Forte Carlo Alberto Veracini 《Polymer Engineering and Science》1998,38(9):1491-1505
The transesterification of poly(ethylene terephthalate) (PET) with a mixture of sebacic acid (S), 4,4′-diacetoxybiphenyl (B) and 4-acetoxybenzoic acid (H), carried out under conditions expectedly favoring the formation of a p(ET-SBH) random copolyester, produces biphasic materials with an isotropic matrix and a highly fibrous, liquid-crystalline dispersed phase. Spectroscopic, calorimetric, microscopic and diffractometric characterization of the fractions separated by solvent extraction has shown that the two phases consist of practically random copolyesters having different average composition. Interestingly, the degree of aromaticity of the matrix is even lower than that of PET, whereas that of the minor phase is appreciably higher than that calculated for the SBH copolyester that would be produced from the monomer mixture in the absence of PET. This unexpected result is interpreted on the basis of an enthalpy-driven progressive diffusion of aromatic-rich material toward the mesophase which segregates at an early stage of the polycondensation within the isotropic mixture of low molar mass oligomers initially produced by the PET acidolysis. Thus, an increasing differentiation, rather than an equilibration, of the composition of the two phases takes place. It is noteworthy that, despite the strong compositional difference, the two phases of these products show fairly good compatibility and interfacial adhesion. 相似文献
64.
65.
Rosalba Lanciotti Francesca Patrignani Luciana Iucci Maria Elisabetta Guerzoni Giovanna Suzzi Nicoletta Belletti Fausto Gardini 《Food chemistry》2007,104(2):693-701
The aim of this work was to evaluate the biogenic amine (BA) content during the ripening of both bovine and ovine cheeses obtained using milk subjected to a homogenization treatment at 100 MPa before cheese-making. The data obtained were compared with those from cheeses produced by the same milks without any treatment or thermized. The results showed that both microbial ecology and BA concentrations of cheeses during ripening were significantly influenced by the type of milk used for cheese-making and by the treatment applied to the raw materials. In particular, the microbial counts found in Caciotta indicated that the high pressure homogenization (HPH) of milk significantly reduced the presence of the yeasts, Micrococcaceae and lactobacilli at the end of ripening. On the other hand, the HPH treatment of milk favoured the proliferation of yeasts in ovine cheese. Moreover, the ovine cheeses were characterized by a remarkably higher accumulation of BA than bovine cheeses. However, the HPH treatment of milk was able to drastically reduce the biogenic amine concentrations in both cheese typologies at the end of ripening. 相似文献
66.
Natale Francesco; Antinucci Francesco; Spinozzi Giovanna; Potí Patrizia 《Canadian Metallurgical Quarterly》1986,100(4):335
Several investigators have reported the achievement of Stage 6 object concept in a number of species of nonhuman primates. Because Stage 6 object concept implies a capacity for mental representation, such an achievement would demonstrate the presence of a crucial capacity in nonhuman primate cognition. There are, it is suggested, problems in the way nonhuman primates have been tested for Stage 6 object concept (i.e., that the tasks employed could be solved in nonrepresentational ways). The present study attempted to differentiate between representational and nonrepresentational responses in the typical task used for testing Stage 6 object concept (following the invisible displacements of an object). The performance of 2 22-mo-old Ss, a Japanese macaque and a gorilla, was compared. It was found that only the gorilla solved the task with the use of mental representation. The macaque consistently tried to solve the task by employing practical, nonrepresentational strategies. (12 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
67.
Giovanna Calabrese Raffaella Giuffrida Debora Lo Furno Nunziatina Laura Parrinello Stefano Forte Rosario Gulino Cristina Colarossi Luciana Rita Schinocca Rosario Giuffrida Venera Cardile Lorenzo Memeo 《International journal of molecular sciences》2015,16(7):15609-15624
The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271− mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271− mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271− mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate. 相似文献
68.
Irene Vanni Simona Coco Anna Truini Marta Rusmini Maria Giovanna Dal Bello Angela Alama Barbara Banelli Marco Mora Erika Rijavec Giulia Barletta Carlo Genova Federica Biello Claudia Maggioni Francesco Grossi 《International journal of molecular sciences》2015,16(12):28765-28782
Next-generation sequencing (NGS) is a cost-effective technology capable of screening several genes simultaneously; however, its application in a clinical context requires an established workflow to acquire reliable sequencing results. Here, we report an optimized NGS workflow analyzing 22 lung cancer-related genes to sequence critical samples such as DNA from formalin-fixed paraffin-embedded (FFPE) blocks and circulating free DNA (cfDNA). Snap frozen and matched FFPE gDNA from 12 non-small cell lung cancer (NSCLC) patients, whose gDNA fragmentation status was previously evaluated using a multiplex PCR-based quality control, were successfully sequenced with Ion Torrent PGM™. The robust bioinformatic pipeline allowed us to correctly call both Single Nucleotide Variants (SNVs) and indels with a detection limit of 5%, achieving 100% specificity and 96% sensitivity. This workflow was also validated in 13 FFPE NSCLC biopsies. Furthermore, a specific protocol for low input gDNA capable of producing good sequencing data with high coverage, high uniformity, and a low error rate was also optimized. In conclusion, we demonstrate the feasibility of obtaining gDNA from FFPE samples suitable for NGS by performing appropriate quality controls. The optimized workflow, capable of screening low input gDNA, highlights NGS as a potential tool in the detection, disease monitoring, and treatment of NSCLC. 相似文献
69.
70.
Condensed tannin accumulation and nitrogen fixation potential of Onobrychis viciifolia Scop. grown in a Mediterranean environment
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