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71.
Sadik OA Xu H Gheorghiu E Andreescu D Balut C Gheorghiu M Bratu D 《Analytical chemistry》2002,74(13):3142-3150
This work describes the theoretical and experimental approaches for monitoring the interfacial biomolecular reaction between immobilized antibody and the antigen binding partner using novel differential impedance spectroscopy. The prerequisite of any biosensor is the immobilization of macromolecules onto the surface of a transducer. It is clear that the function of most macromolecules changes from what is observed in solution once immobilization has occurred. In the worst case, molecules entirely lose their binding activity almost immediately after immobilization. Certain conditions (e.g., denaturation, interfacial effects based on ionic strength, surface charge, dielectric constants, etc.) at interfaces are responsible for alterations of binding activity; it is not clear whether a combination of such processes is understood. However, these processes in combination must be reliably modeled in order to predict the outcome for most macromolecules. This work presents the theoretical and practical means for elucidating the surface reactivity of biomolecular reagents using ion displacement model with antibody-antigen (Ab-Ag) reaction as the test case. The Ab-Ag reaction was directly monitored using a dual-channeled, impedance analyzer capable of 1 measurement/s using covalent immobilization chemistry and polymer-modified electrodes in the absence of a redox probe. The evidence of Ab-Ag binding was revealed through the evolution of differential admittance. The surface loading obtained using the covalent immobilization chemistry was 9.0 x 10(16)/cm2, whereas with polymer-modified electrodes, the surface loading was 9.0 x 10(15)/cm2, representing a 10 times increase in surface reactivity. The proposed approach may be applicable to monitoring other surface interfacial reactions such as DNA-DNA interactions, DNA-protein interactions, and DNA-small molecule interactions. 相似文献
72.
Sarmad Sadik Alade Rahman Arshad Ali H. Farooq Ahmad Hiroki Suguri 《The Journal of supercomputing》2010,52(2):97-118
Disaster management systems are complex applications due to their distributed and decentralized nature. Various components
execute in parallel with high need of coordination with each other. In such applications, interaction and communication issues
are difficult to model and implement. In this paper, we have proposed agent-based Earthquake Management System (EMS) which
is modeled and analyzed using formal approach. Traditionally, such systems undergo through various transformations starting
from requirement models and specification to analysis, design and implementation. A variety of formal approaches are available
to specify systems for analyzing their structure and behavior; however, there are certain limitations in using these techniques
due to their expressiveness and behavior requirements. We have adopted combination of Pi-calculus and Pi-ADL formal languages
to model EMS from analysis to design. The formal approach helps to enhance reliability and flexibility of the system by reducing
the redundant information. It reduces chances of errors by explicitly mentioning working flow of information. Additionally,
a prototype application is presented as proof of concept in EMS context. We have also evaluated our formal specification by
using ArchWare and ABC tools; also, comparison of prototype application with major existing techniques is highlighted. 相似文献
73.
Understanding of the role of energy use at the national level requires the understanding of the relationship of energy use to economic activity and social well-being. Gross domestic product (GDP) measures the value of goods and services produced in a country in one year. There is a close relationship between energy supply, energy consumption, and GDP, which indicates the economic development of a country. The living standard of a country is often measured by the per capita GDP. This article presents the evaluations and future projections of energy and energy resources of the Organization for Economic Cooperation and Development (OECD). The total primary energy supply, total final energy consumption, and energy intensities for supply and consumption are analysed. The energy data for all OECD countries are presented and analyses of the differences in energy and GDP ratios are conducted at an aggregate level by examining differences in the factors that affect the energy intensities. To provide accurate projections for the future, new correlations are developed between average GDP, total primary energy supply, total final consumption, total per capita primary energy supply, total per capita final consumption and total OECD population. © 1997 by John Wiley & Sons, Ltd. 相似文献
74.
The article presents a simple technique for the purpose of heat and mass transfer analysis in an ACS. The LiBr/water ACS was selected as an example. based on optimum operation conditions (i.e., the maximum cooling temperature delivered by the ACS), simple but effective expression were derived in terms of the mass flow rate of absorbent—the most important parameter for practical design calculations. The results show that the proposed technique provides, for practical application purposes, a useful tool for a general analysis of the ACS as well as for the system design purpose. 相似文献
75.
Nusrat Lubna Zariff Chaudhury Golam Newaz 《Journal of Materials Engineering and Performance》2012,21(9):1962-1969
For miniaturized biomedical devices, laser joining of dissimilar materials offers excellent potential to make precise joints. An important system for consideration is titanium (Ti) coated glass joined with biocompatible imidex polyimide (PI). Metallic Ti with various thicknesses was deposited on top of pyrex 7740 borosilicate glass by using DC-magnetron sputtering deposition method. Effect of bond strength between Ti coated glass and imidex polyimide (PI), due to thickness variation of sputtered Ti coating was studied. Three different Ti inter-layer thicknesses were considered, 50, 200, and 400?nm. Tests results indicated that the thinner film produced lower shear strength and higher thickness produced higher shear strength. It has been observed that thicker film (200 and 400?nm) enhanced considerably the bond strength with enhancing the film roughness as well. Higher roughness resulted in more contact area at the interface, results higher number of chemical bonds and increased mechanical interlocking; which in turn increase the laser joint strength. For stronger bond with higher thickness, mixed mode failure was observed which included cohesive failure of polymer, interface failure of Ti/glass and failure on the glass itself. On the other hand, for weak bond with thinner film, mostly interface failure was observed for this system of Ti coated glass/imidex. For thicker film, chemical bond of Ti-C and Ti-O were observed. The role of both surface characteristics and chemical bonding for laser joints were investigated by using advanced techniques such as X-ray photoelectron spectroscopy, scanning electron microscopy, and energy dispersive spectroscopy. 相似文献
76.
In this study, different welding parameters were applied to two different steels with high alloys and mechanical and metallographical investigations are performed. Thus, the optimum welding parameters were determined for these materials and working conditions. 12·30 diameter steel bars made up of 1·4871 (X53CrMnNiN219) and 1·4718 (X45CrSi93) steel were used as experimental material. The material loss increased with increase in friction and rotating pressure. No fracture at the welding region was observed and the highest fracture energy was identified in B5 group. Based on micro hardness investigation; the hardness profile reached its minimum value at the welding region. 相似文献
77.
78.
Semiconductor Nanowire Light‐Emitting Diodes Grown on Metal: A Direction Toward Large‐Scale Fabrication of Nanowire Devices 下载免费PDF全文
ATM Golam Sarwar Santino D. Carnevale Fan Yang Thomas F. Kent John J. Jamison David W. McComb Roberto C. Myers 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(40):5402-5408
Bottom‐up nanowires are attractive for realizing semiconductor devices with extreme heterostructures because strain relaxation through the nanowire sidewalls allows the combination of highly lattice mismatched materials without creating dislocations. The resulting nanowires are used to fabricate light‐emitting diodes (LEDs), lasers, solar cells, and sensors. However, expensive single crystalline substrates are commonly used as substrates for nanowire heterostructures as well as for epitaxial devices, which limits the manufacturability of nanowire devices. Here, nanowire LEDs directly grown and electrically integrated on metal are demonstrated. Optical and structural measurements reveal high‐quality, vertically aligned GaN nanowires on molybdenum and titanium films. Transmission electron microscopy confirms the composition variation in the polarization‐graded AlGaN nanowire LEDs. Blue to green electroluminescence is observed from InGaN quantum well active regions, while GaN active regions exhibit ultraviolet emission. These results demonstrate a pathway for large‐scale fabrication of solid state lighting and optoelectronics on metal foils or sheets. 相似文献
79.
Sadik Buyukyoruk Naim D Ayaz Yilmaz E Gencay Devrim Beyaz Pelin Kocak 《International Journal of Dairy Technology》2014,67(1):103-109
Species distribution, virulence traits and vancomycin resistance gene profiles of Enterococcus isolated from 43 home‐made artisan cheese samples collected from open markets, located in Aydin region of Turkey, were investigated. Of the 129 isolates, 95 were identified as Enterococcus sp.; Enterococcus faecium being the most prevalent species (82.1%), followed by Enterococcus faecalis (13.6%) and Enterococcus durans (1.0%). None of the enterococci were harbouring vanA or vanC, while seven isolates (7.3%) were shown to harbour vanB gene by multiplex PCR. gelE (49.4%) being the most prevalent virulence factor was followed by asa1 (27.3%), esp (22.1%), cylA (4.2%) and hyl (3.1%). 相似文献
80.
Localized surface plasmon resonances in silver and gold nanostructures are engaged to enhance the inelastic Raman scattering and the fluorescence of a phopholipid containing a sulforhodamine 101 acid chloride dye known as Texas Red. The most efficient coupling and enhancement are attained when the excitation laser line is in resonance with both the chromophore and the plasmon absorption of the nanostructure, the case of surface-enhanced resonance Raman scattering, allowing single-molecule detection. The tagged phospholipid was incorporated into a single fatty acid Langmuir monolayer at varying concentrations and transferred onto an evaporated Ag nanoparticle film. Surface-enhanced fluorescence is achieved using shell-isolated silica-coated gold nanoparticles, an emission enhancement named SHINEF. 相似文献