Activation of the NLRP3 Inflammasome Increases the IL-1β Level and Decreases GLUT4 Translocation in Skeletal Muscle during Insulin Resistance

Low-grade chronic inflammation plays a pivotal role in the pathogenesis of insulin resistance (IR), and skeletal muscle has a central role in this condition. NLRP3 inflammasome activation pathways promote low-grade chronic inflammation in several tissues. However, a direct link between IR and NLRP3 inflammasome activation in skeletal muscle has not been reported. Here, we evaluated the NLRP3 inflammasome components and their role in GLUT4 translocation impairment in skeletal muscle during IR. Male C57BL/6J mice were fed with a normal control diet (NCD) or high-fat diet (HFD) for 8 weeks. The protein levels of NLRP3, ASC, caspase-1, gasdermin-D (GSDMD), and interleukin (IL)-1β were measured in both homogenized and isolated fibers from the flexor digitorum brevis (FDB) or soleus muscle. GLUT4 translocation was determined through GLUT4myc-eGFP electroporation of the FBD muscle. Our results, obtained using immunofluorescence, showed that adult skeletal muscle expresses the inflammasome components. In the FDB and soleus muscles, homogenates from HFD-fed mice, we found increased protein levels of NLRP3 and ASC, higher activation of caspase-1, and elevated IL-1β in its mature form, compared to NCD-fed mice. Moreover, GSDMD, a protein that mediates IL-1β secretion, was found to be increased in HFD-fed-mice muscles. Interestingly, MCC950, a specific pharmacological NLRP3 inflammasome inhibitor, promoted GLUT4 translocation in fibers isolated from the FDB muscle of NCD- and HFD-fed mice. In conclusion, we found increased NLRP3 inflammasome components in adult skeletal muscle of obese insulin-resistant animals, which might contribute to the low-grade chronic metabolic inflammation of skeletal muscle and IR development.     

Entire Life Time Monitoring of Filament Wound Composite Cylinders Using Bragg Grating Sensors: II. Process Monitoring

This article is the second of a series of three papers concerning monitoring of filament wound cylinders using Bragg gratings. In this second part, the tooling presented in Part I is used to embed gratings and thermocouples in filament wound glass reinforced epoxy composite cylinders during fabrication. Bragg grating strain was obtained from wavelength and temperature response, by a calibration technique described here. Results from tests on five cylinders show the Bragg grating’s capability to monitor strain evolution during fabrication, and the capacity to detect several phenomena occurring during cure is established, in addition to quantifying the initial material condition of the cylinder before it enters service.     

A new engine indicating measurement procedure for combustion heat release analysis

This paper presents a new arrangement for the indicating measurement system utilized in the study of internal combustion engines. In this experimental configuration, the current polarized by a piezoelectric pressure transducer is converted into an analog signal proportional to the cylinder pressure variation rate, which constitutes the primary information for combustion heat release analysis. The proposed technique reduces the uncertainty in the pressure derivative data, providing more accurate heat release results than those obtained with the traditional transducer signal conditioning procedure, which requires the numerical derivation of in-cylinder pressure data supplied by a charge amplifier.     

DNA damage detection technique applying time-resolved fluorescence measurements

A novel DNA damage detection technique based on the characteristic fluorescence lifetimes exhibited by Pico-Green-single-stranded DNA and -double-stranded DNA complexes is employed to establish the damage produced on DNA isolated from sheep white blood cells following gamma radiation. This technique, which incorporates key concepts such as alkaline unwinding buffers and higher unwinding rates of damaged DNA compared to undamaged DNA, allows for the differentiation of DNA damage resulting from doses of gamma radiation in the 0-100-Gy range, with the potential of analyzing samples consisting of as little as 10(4) cells. Experiments were carried out using commercial DNA sources as well as DNA isolated from sheep white blood cells, suggesting its potential for use with isolated DNA from virtually any eukaryotic cell.     

Switching class labels to generate classification ensembles

Ensembles that combine the decisions of classifiers generated by using perturbed versions of the training set where the classes of the training examples are randomly switched can produce a significant error reduction, provided that large numbers of units and high class switching rates are used. The classifiers generated by this procedure have statistically uncorrelated errors in the training set. Hence, the ensembles they form exhibit a similar dependence of the training error on ensemble size, independently of the classification problem. In particular, for binary classification problems, the classification performance of the ensemble on the training data can be analysed in terms of a Bernoulli process. Experiments on several UCI datasets demonstrate the improvements in classification accuracy that can be obtained using these class-switching ensembles.     

Effective bandwidth estimation and testing for Markov sources

This work addresses the resource sharing problem in broadband communication networks that can guarantee some quality of service (QoS), and develops some results about data source and traffic modelling, especially in aspects of model testing and parameter estimation. The multiplexing of variable bit rate (VBR) sources poses a mathematical and statistical problem: the estimation of the resource requirements of a source or set of sources. The estimation method shall be simple enough to be practically implemented in the connection acceptance control (CAC) function.

In this paper, the VBR video sources are taken as a typical case of variable rate, with real-time constraints. This association of requirements makes the case especially interesting. A Markov model is assumed for the VBR sources. The validity of such models is under research; they seem to be appropriate at least in certain time scales. The model is tested against real video traces. In order to estimate the resource allocation or “channel occupation” of each source, the concept of equivalent bandwidth proposed by Kelly [Notes on effective bandwidth, in: F.P. Kelly, S. Zachary, I.B. Ziedins (Eds.), Stochastic Networks: Theory and Applications, Oxford University Press, Oxford, 1996, pp. 141] is used; it is based on a consistent mathematical theory, and has proven to be robust and useful for technical applications.

A calculation of the equivalent bandwidth of a Markov source, given its parameters, can be found in the literature [IEEE ACM Trans. Networking 1 (4) (1993) 424]. But in fact, one can only estimate model and parameters. In this work, an estimation of the equivalent bandwidth is given, which can be obtained from real data. The convergence and the consistency of the estimation are studied, and practical bounds are found. Illustrative calculations are performed from real video traces that were obtained using a software MPEG coder, developed by the authors. The mathematical and statistical results are valid for whatever phenomenon that can be modelled as a Markov process.     

Comparison of linear and rotationally shearing interferometric layouts for extrasolar planet detection from space

We use the point spread function and the modulation transfer function (MTF) as two figures-of-merit to evaluate the performance of the multiaperture interferometric configurations for the detection of a faint planet in the vicinity of its bright star. We design nonredundant interferometric layouts that provide satisfactory coverage of the spatial frequencies of interest. We propose a design incorporating a rotating, rotationally shearing interferometer in a gravity-free environment and compare its performance with the Earth-based, fixed, linear configurations. The side peak of its MTF may be centered on the coordinate associated with a likely planet spatial frequency, resulting in planet signal enhancement and isolation.     

Modeling and Analysis of Composite Antenna Superstrates Consisting on Grids of Loaded Wires

We study the characteristics and radiation mechanism of antenna superstrates based on closely located periodical grids of loaded wires. An explicit analytical method based on the local field approach is used to study the reflection and transmission properties of such superstrates. It is shown that as a result of proper impedance loading there exists a rather wide frequency band over which currents induced to the grids cancel each other, leading to a wide transmission maximum. In this regime radiation is produced by the magnetic dipole moments created by circulating out-of-phase currents flowing in the grids. An impedance matrix representation is derived for the superstrates, and the analytical results are validated using full-wave simulations. As a practical application example we study numerically the radiation characteristics of dipole antennas illuminating finite-size superstrates.     

Ultrasound image segmentation with shape priors: application to automatic cattle rib-eye area estimation.

Automatic ultrasound (US) image segmentation is a difficult task due to the quantity of noise present in the images and the lack of information in several zones produced by the acquisition conditions. In this paper, we propose a method that combines shape priors and image information to achieve this task. In particular, we introduce knowledge about the rib-eye shape using a set of images manually segmented by experts. A method is proposed for the automatic segmentation of new samples in which a closed curve is fitted taking into account both the US image information and the geodesic distance between the evolving curve and the estimated mean rib-eye shape in a shape space. This method can be used to solve similar problems that arise when dealing with US images in other fields. The method was successfully tested over a database composed of 610 US images, for which we have the manual segmentations of two experts.     

Oxidation of amino groups by hydroxyl radicals in relation to the oxidation degree of the alpha-carbon

Nitrogen organic compounds constitute a large class of aqueous pollutants. These compounds include not only azoic structures, nitrogen heterocycles, and nitrous groups but also amides and amines. This work consisted in studying the OH* induced oxidation of simple primary amines in dilute aqueous solution with special attention to mineralization of the nitrogen group as a function of the nature of the alpha-carbon. H2O2/UV and gamma-irradiation processes were used for the production of OH* radicals, and the molecules studied were one alpha-amino acid i.e., glycine (HOOCCH2NH2), and two primary amides i.e., acetamide (CH3CONH2) and oxamic acid (HOOCCONH2). It was shown that the oxidation of glycine leads to the formation of ammonia, whereas the acetamide molecule is first oxidized into oxamic acid ending in complete mineralization with production of nitrates. Reaction mechanisms are proposed which account for the observed inorganic nitrogen end product depending on the oxidation degree of the carbon atoms of the molecules. It follows that the present study will allow for prediction of the fate of nitrogen resulting from the oxidation of primary amino groups by OH* radicals.