Pilot experiments with electrodialysis and ozonation for the production of a fertiliser from urine.

Pilot tests were performed with a process combination of electrodialysis and ozonation for the removal of micropollutants and the concentration of nutrients in urine. In continuous and batch experiments, maximum concentration factors up to 3.5 and 4.1 were obtained, respectively. The desalination capacity did not decrease significantly during continuous operation periods of several weeks. Membrane cleaning after 195 days resulted in approximately 35% increase in desalination rate. The Yeast Estrogen Screen (YES), a bioassay that selectively detects oestrogenic compounds, confirmed that about 90% of the oestrogenic activity was removed by electrodialysis. HPLC analysis showed that ibuprofen was removed to a high extent, while other micropollutants were below the detection limit. In view of the fact that ibuprofen is among the most rapidly transported micropollutants in electrodialysis processes, this result indicates that electrodialysis provides an effective barrier for micropollutants. Standardised plant growth tests were performed in the field with the salt solution resulting from the treatment by electrodialysis and subsequent ozonation. The results show that the plant height is comparable to synthetic fertilisers, but the crop yield is slightly lower. The latter is probably caused by volatilisation losses during field application, which can be prevented by improved application technologies.     

Intramyocardial Inflammation after COVID-19 Vaccination: An Endomyocardial Biopsy-Proven Case Series

Myocarditis in response to COVID-19 vaccination has been reported since early 2021. In particular, young male individuals have been identified to exhibit an increased risk of myocardial inflammation following the administration of mRNA-based vaccines. Even though the first epidemiological analyses and numerous case reports investigated potential relationships, endomyocardial biopsy (EMB)-proven cases are limited. Here, we present a comprehensive histopathological analysis of EMBs from 15 patients with reduced ejection fraction (LVEF = 30 (14–39)%) and the clinical suspicion of myocarditis following vaccination with Comirnaty^® (Pfizer-BioNTech) (n = 11), Vaxzevria^® (AstraZenica) (n = 2) and Janssen^® (Johnson & Johnson) (n = 2). Immunohistochemical EMB analyses reveal myocardial inflammation in 14 of 15 patients, with the histopathological diagnosis of active myocarditis according the Dallas criteria (n = 2), severe giant cell myocarditis (n = 2) and inflammatory cardiomyopathy (n = 10). Importantly, infectious causes have been excluded in all patients. The SARS-CoV-2 spike protein has been detected sparsely on cardiomyocytes of nine patients, and differential analysis of inflammatory markers such as CD4⁺ and CD8⁺ T cells suggests that the inflammatory response triggered by the vaccine may be of autoimmunological origin. Although a definitive causal relationship between COVID-19 vaccination and the occurrence of myocardial inflammation cannot be demonstrated in this study, data suggest a temporal connection. The expression of SARS-CoV-2 spike protein within the heart and the dominance of CD4⁺ lymphocytic infiltrates indicate an autoimmunological response to the vaccination.     

Face to virtual face

The first virtual humans appeared in the early 1980s in such films as Dreamflight (1982) and The Juggler (1982). Pioneering work in the ensuing period focused on realistic appearance in the simulation of virtual humans. In the 1990s, the emphasis has shifted to real-time animation and interaction in virtual worlds. Virtual humans have begun to inhabit virtual worlds and so have we. To prepare our place in the virtual world we first develop techniques for the automatic representation of a human face capable of being animated in real time using both video and audio input. The objective is for one's representative to look, talk, and behave like oneself in the virtual world. Furthermore, the virtual inhabitants of this world should be able to see our avatars and to react to what we say and to the emotions we convey. We sketch an overview of the problems related to the analysis and synthesis of face-to-virtual-face communication in a virtual world. We describe different components of our system for real-time interaction and communication between a cloned face representing a real person and an autonomous virtual face. It provides an insight into the various problems and gives particular solutions adopted in reconstructing a virtual clone capable of reproducing the shape and movements of the real person's face. It includes the analysis of the facial expression and speech of the cloned face, which can be used to elicit a response from the autonomous virtual human with both verbal and nonverbal facial movements synchronized with the audio voice     

Rosemary Extract and Precooking Effects on Lipid Oxidation in Heat-Sterilized Meat

The influence of precooking and addition of refined rosemary extract on lipid oxidation during storage of heat-sterilized meat in trays was investigated. Short precooking resulted in increased lipid oxidation, while more extended precooking using peanut oil gave products with higher stability during storage. The addition of a gravy-type sauce (instead of water) to the meat when filling it into trays also resulted in higher storage stability. However, only the application of rosemary extract, especially after precooking, resulted in products with stability similar to those packed with nitrogen flushing.     

pH dependence of the partitioning of triphenyltin and tributyltin between phosphatidylcholine liposomes and water

Triorganotin compounds are very toxic contaminants. The site of their basic mechanism of action of acute toxicity is the biomembrane. Liposome-water distribution ratios of triphenyltin and tributyltin were determined between pH 3 and pH 8 with the equilibrium dialysis method in the micromolar concentration range, which is the concentration range where acute toxicity is observed. In addition, biomembrane-water distribution ratios of tributyltin were determined with chromatophores of Rhodobacter sphaeroides that contain approximately 70% protein intercalated in the lipid bilayer. The liposome-water distribution of both compounds showed only weak pH dependence. For tributyltin, the apparent distribution ratio decreased from 4100 at low pH to 2000 at high pH, while this ratio decreased from 70 000 to 22 000 for TPT. The distribution ratio of the triorganotin cation exceeded that of the neutral hydroxo complex by a factor of 2. The distribution ratio of both the cation and the hydroxo complex of triphenyltin exceeded that of tributyltin by a factor of 10. It is postulated that the sorption of the cation is governed by complex formation with ligands in the phospholipids, presumably the phosphate group. The biomembrane-water distribution ratio of tributyltin was found to be lower than the liposome-water distribution ratio at high pH. The hydroxo complex appears to partition only to the lipid fraction of the biomembrane. Yet, at low pH the biomembrane-water distribution ratio exceeded the liposome-water distribution ratio, which is attributed to complex formation of the cationic species with ligands of the protein fraction.     

Business owners' action planning and its relationship to business success in three African countries.

A model of business success was developed with motivational resources (locus of control, self-efficacy, achievement motivation, and self-reported personal initiative) and cognitive resources (cognitive ability and human capital) as independent variables, business owners' elaborate and proactive planning as a mediator, and business size and growth as dependent variables. Three studies with a total of 408 African micro and small-scale business owners were conducted in South Africa, Zimbabwe, and Namibia. Structural equation analyses partially supported the hypotheses on the importance of psychological planning by the business owners. Elaborate and proactive planning was substantially related to business size and to an external evaluation of business success and was a (partial) mediator for the relationship between cognitive resources and business success. The model carries important implications for selection, training, and coaching of business owners. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Natural versus wastewater derived dissolved organic carbon: implications for the environmental fate of organic micropollutants

The interaction of organic micropollutants with dissolved organic carbon (DOC) can influence their transport, degradation and bioavailability. While this has been well established for natural organic carbon, very little is known regarding the influence of DOC on the fate of micropollutants during wastewater treatment and water recycling. Dissolved organic carbon-water partition coefficients (K_DOC) for wastewater derived and reference DOC were measured for a range of micropollutants using a depletion method with polydimethylsiloxane disks. For micropollutants with an octanol-water partition coefficient (log K_OW) greater than 4 there was a significant difference in K_DOC between reference and wastewater derived DOC, with partitioning to wastewater derived DOC over 1000 times lower for the most hydrophobic micropollutants. The interaction of nonylphenol with wastewater derived DOC from different stages of a wastewater and advanced water treatment train was studied, but little difference in K_DOC was observed. Organic carbon characterisation revealed that reference and wastewater derived DOC had very different properties due to their different origins. Consequently, the reduced sorption capacity of wastewater derived DOC may be related to their microbial origin which led to reduced aromaticity and lower molecular weight. This study suggests that for hydrophobic micropollutants (log K_OW > 4) a higher concentration of freely dissolved and thus bioavailable micropollutants is expected in the presence of wastewater derived DOC than predicted using K_DOC values quantified using reference DOC. The implication is that naturally derived DOC may not be an appropriate surrogate for wastewater derived DOC as a matrix for assessing the fate of micropollutants in engineered systems.     

Elimination of beta-blockers in sewage treatment plants

beta-Blockers are used to treat high blood pressure as well as patients recovering from heart attacks. In several studies, they were detected in surface water, thus indicating incomplete degradability of these substances in sewage treatment plants (STPs). In this study, we determined the sorption coefficients (K(D)) and degradation rates of the four beta-blockers sotalol, atenolol, metoprolol and propranolol in sludge from an STP operating with municipal wastewater. The sorption coefficients (K(D), standard deviations in brackets) were determined as 0.04(+/-0.035), 0.04(+/-0.033), 0.00(+/-0.023) and 0.32(+/-0.058) Lg(-1)(COD), and the pseudo-first-order degradation rate constants were estimated to be 0.29(+/-0.02), 0.69(+/-0.05), 0.58(+/-0.05) and 0.39(+/-0.07) Ld(-1)g(-1)(COD) for sotalol, atenolol, metoprolol and propranolol, respectively. These values translate into a typical elimination in STPs (sludge concentrations of 4g(COD)L(-1) and a hydraulic retention time of 6h) of 25%, 37%, 44% and 50% for sotalol, propranolol, metoprolol and atenolol, respectively. These results are also confirmed by measurements in two municipal STPs for atenolol, sotalol and propranolol. The estimated eliminations are slightly too high for metoprolol.     

Some thoughts on the aquarius program: A prospective world effort in hydrogen-energy

Increasing discussion of what actions the US, and the world community generally, can take in the sphere of energy is being heard. The tempo is picking up; mention of 'lscrash programme’ possibilities is even made. Now may be the time to bring forward focused ideas, especially those which may make long-term sense. One such idea is that of a ‘hydrogen-energy system’ about which much has been written and spoken, but little yet accomplished in the sense of physical development.The United States is well known for its ability to establish an effective large-scale programme, once the stage of ‘determination’ has been reached, usually late-in-the-day in the sense of our facing up to the basic problem. This paper sketches out a ‘program-matistion’ of the hydrogen-energy concept somewhat in the spirit of the Apollo Program with which there are some parallels. But ‘Aquarius’ would be a much more ambitious and longer termed programme. It would involve practically all US citizens and, hopefully, gain international participation. Expensive? Of course. But the monies spent are with the identical constituency who would profit from the transition from fossil fuels.