Lipid production in Porphyridium cruentum grown under different culture conditions

Autotrophic growth of Porphyridium cruentum under 18:12 h and 12:12 h light:dark cycles showed the maximum cell concentration of 2.1 g-dry wt./L, whereas the specific growth rate, 0.042 (1/h), at 18:6 h is faster than that of 12:12 h, 0.031 (1/h), respectively. The highest lipid accumulation level, 19.3 (%, w/w), was achieved at 12:12 h cycle. Under dark cultivation condition with 10 g/L of glucose, the lipid accumulation in the cell was 10.9 (%, w/w), whereas the heterotrophic growth with glycerol as the carbon resource showed low level of cell concentration and lipid production, compared to that of glucose. The glucose was decided to be a suitable carbon resource for the heterotrophic growth of P. cruentum. The lipids from P. cruentum seemed be feasible for biodiesel production, because over 30% of the lipid was C₁₆–C_18:1. The cultivation time and temperature were important factors to increase the maximum cell concentration. Extending the cultivation time helps maintain the maximum cell concentration, and higher lipid accumulation was achieved at 25 °C, compared to 35 °C. The fed-batch cultures showed that, under the light condition, the specific production rate was slightly decreased to 0.4% lipid/g-dry wt./day at the later stage, whereas, under the dark condition, the specific production rate was maintained to be a maximum value of 1.1% lipid/g-dry wt./day, even in the later stage of cultivation. The results indicate that the heterotrophic or 12:12 h cyclic mixotrophic growth of P. cruentum could be used for the production of biodiesel in long-term fed-batch cultivation of P. cruentum.     

Template-Free Preparation of a Mesopore-Rich Hierarchically Porous Carbon Monolith from a Thermally Rearrangeable Polyurea Network

A mesopore-rich, hierarchically porous carbon monolith was prepared by carbonizing a polyisocyanurate network derived by thermal rearrangement of a polyurea network. The initial polyurea network was synthesized by the cross-linking polymerization of tetrakis(4-aminophenyl)methane (TAPM) and hexamethylene diisocyanate (HDI) in the sol-forming condition, followed by precipitation into nanoparticulate solids in a nonsolvent. The powder was molded into a shape and then heated at 200–400 °C to obtain the porous carbon precursor composed of the rearranged network. The thermolysis of urea bonds to amine and isocyanate groups, the subsequent cyclization of isocyanates to isocyanurates, and the vaporization of volatiles caused sintering of the nanoparticles into a monolithic network with micro-, meso-, and macropores. The rearranged network was carbonized to obtain a carbon monolith. It was found that the rearranged network, with a high isocyanurate ratio, led to a porous carbon with a high mesopore ratio. The electrical conductivity of the resulting carbon monoliths exhibited a rapid response to carbon dioxide adsorption, indicating efficient gas transport through the hierarchical pore structure.     

Effect of substrate on the phase transformation of TiO2 in pearlescent pigment

The TiO₂/substrate pearlescent pigments were prepared by the hydrolysis of TiOCl₂ on the substrate followed by a calcinations process. The natural mica (muscovite), synthetic mica (fluorophlogopite) and -alumina flake were selected as the substrates for pearlescent pigments. The effect of substrate on the anatase to rutile (A–R) phase transformation of TiO₂ was studied. The A–R phase transformation of TiO₂ during the preparation of pearlescent pigments and their proportion in the TiO₂ layer have been analyzed by XRD measurements. The phase compositions of TiO₂ layer in each pearlescent pigment are quite different depending on the substrates. The TiO₂ layer deposited on -alumina has higher rutile fraction than those on the natural and synthetic mica. The XPS analysis showed that the cations originally present in the substrates diffused into the TiO₂ layer. The TiO₂ layer deposited on -alumina contains Al, while those on the natural and synthetic mica substrates contain Si and K in addition to Al. The metal cations diffusing from the substrate into TiO₂ layer might retard the A–R phase transformation of TiO₂. The suppressing effect on the A–R transformation of TiO₂ by mixed cations seems to be much stronger than that of single cation, resulting in relatively higher rutile fraction in the case of TiO₂ layer deposited on -alumina.     

Experimental research on thermal comfort in the university classroom of regular semesters in Korea

This research has investigated physical variables affecting indoor thermal comfort and subjective responses of thermal comfort of students in a university in Korea in which the weather is oceanic temperate climate, and has been performed to contribute to the research fields of Sustainable Thermal Standard and Adaptive Thermal Comfort (ATC). This research is based on the ISO 7730-2005 standard and the ATC theories and 4 main variables of PMV such as dry bulb temperature (Ta), relative humidity (RH), black bulb temperature (Tg), and air velocity (Va) are measured once a week during two regular semesters. A clothing insulation, a thermal sensation vote (TSV), an acceptability of thermal environment, and a preference for cooling and heating are investigated at the same time using a questionnaire. This study was carried out for 26 weeks during the spring season, from March to June 2009, and the autumn season, from September to December 2009. The main achievements of this study are as follows. Monthly Mean Outdoor Temperature (MMOT) and Operative Temperature (OT) in the classroom during research periods are 7.4∼23.3°C and 17.5∼29.0°C, respectively. The acceptability ratio of thermal environment shows over 80% when the range of OT in the classroom is 17∼25°C, and the range can be applicable to operative index of heating and cooling of classroom. The mean TSV of respondents is almost “neutral (0)” when the PMV in the classroom moves to “neutral (0)” and “slightly cool (−1)”, and the TSV is almost “+1.5” when the PMV moves to “slightly warm (+1)”. The acceptability ratio of thermal environment is slightly different from ASHRAE Standard 55-2004. So it is necessary to more investigate standard range of acceptability of thermal environment in oceanic temperate climate region using much more databases.     

Reverse‐Conducting IGBT Using MEMS Technology on the Wafer Back Side

In this paper, we present a 600‐V reverse conducting insulated gate bipolar transistor (RC‐IGBT) for soft and hard switching applications, such as general purpose inverters. The newly developed RC‐IGBT uses the deep reactive‐ion etching trench technology without the thin wafer process technology. Therefore, a freewheeling diode (FWD) is monolithically integrated in an IGBT chip. The proposed RC‐IGBT operates as an IGBT in forward conducting mode and as an FWD in reverse conducting mode. Also, to avoid the destructive failure of the gate oxide under the surge current and abnormal conditions, a protective Zener diode is successfully integrated in the gate electrode without compromising the operation performance of the IGBT.     

Rule-based personalized comparison shopping including delivery cost

Comparison shopping allows customers to reduce time and effort when searching for product information and prices. However, traditional comparison sites mainly compare product prices without using precise information on delivery cost. To overcome this limitation, we adopted a rule-based comparison shopping framework using the eXtensible Rule Markup Language (XRML) architecture, which computes the exact personalized delivery cost at comparison sites. The prototype ConsiderD, which was developed for this purpose, computes the exact delivery costs considering the shipping rules, destination, delivery speed, and shipping rates. The XRML architecture effectively maintains the consistency of formal rules with the original Web pages. To demonstrate the performance of rule-based comparisons, we conducted an experiment on the purchase of books based on real-world data from five leading online bookstores. This experiment shows that rule-based comparison can significantly outperform data-based comparison in terms of the total cost of product and delivery. We also found that the comparison of delivery cost is very important because the variance of delivery cost can be as big as the variance of book prices itself.     

Nonlinear properties analysis on rutting behaviour of bituminous materials with different air void contents

Rutting is one of the major distresses of flexible pavement. It is defined as the formation of longitudinal depressions along the wheel paths caused by the progressive movement of materials under traffic loading in the asphalt pavement layers or in the underlying base through consolidation or plastic flow. This structural damage has a negative financial impact to the economy. In this study, the rutting behaviour of bituminous materials with different air void contents was investigated. The dynamic cyclic compression testing was carried out to establish nonlinear material models with multiple regression technique. With the specified material models, finite element analysis was carried out to study the rutting behaviour of the wearing course materials with different air void contents in a flexible pavement structure. The simulation result shows that the rutting depth is small at the air void contents of 4.5–8% for wearing course materials. However, for the air void contents above or below this range, the rutting resistance reduces, and thus the rutting depth increases. To verify this simulation result, wheel tracking tests were performed to obtain laboratory data, and the test data was found to be very close to the simulated one. This proved that the developed nonlinear model is applicable to simulate the rutting performance of bituminous mixture and it is a convenient and economical method to be used for the design of bituminous mixtures for both new and rehabilitated pavements.     

Fluorescent dye labeled bacteriophages--a new tracer for the investigation of viral transport in porous media: 1. Introduction and characterization

A new method for the study of pathogen transport in porous media is presented. The method is based on conjugation of fluorescent dyes to target bacteriophages and application of the modified bacteriophages for tracer studies. We demonstrate that the relevant transport determining properties of Rhodamine and several fluorescein-labeled phages are practically identical to those of the native bacteriophages. The advantages of the proposed method relative to direct enumeration of bacteriophages by plaque forming unit method, turbidity, fluorescent microspheres, and other alternative tracers are discussed. Notable advantages include simple quantitation by optical methods, unbiased signals even when virus aggregates are formed, and the ability to decouple inactivation kinetics from transport phenomena. Additionally, the signal reflects the removal and transport of the studied microorganism and not a surrogate.     

Organically modified silica sol-mediated capillary electrophoresis

We describe in this paper the use of ormosil (organically modified silica) sols as additives to the run buffer for selectivity manipulations between solutes in capillary electrophoresis. CE systems that contain sol additives in the run buffer can be thought of as pseudocapillary electrochromatography. Three sols based on different types of silanes were studied. Methyltrimethoxysilane (MTMOS)-based sol was found to improve selectivities between various aromatic acids. Aminopropyltrimethoxysilane (APS) sol interacts differently with structural isomers of aromatic acids than does MTMOS. At low pH with APS sol in the run buffer, neutral solutes can be separated, as well. The separation of the neutral solutes seems to be facilitated by the formation of hydrogen bonds between the solutes and the APS sol. APS and N-[3-(trimethoxysilyl)propyl]-ethylenediamine (EDAS) affect the separation of the same compounds differently, thus indicating that even small changes of the functional groups of the sol have pronounced effect on the interactions between the sols and the solutes.