不同光质对苦荞萌发过程中的黄酮类化合物及其抗氧化活性的影响

本文以萌发3 d的苦荞为实验材料,通过红光、黄光、绿光、白光、蓝光、紫外A（254 nm）、紫外C（365 nm）七种不同的光质处理萌发过程中的苦荞,研究了不同光质对萌发苦荞中黄酮类化合物、抗氧化活性以及与黄酮合成和降解有关酶类的影响。实验结果表明:经紫外、蓝光、白光、绿光、黄光和红光处理均可显著地增加苦荞芽中总黄酮和芦丁的含量（与暗处理比较）,不同处理之间有显著性差异（p<0.05）。C段紫外（UV-C）处理后,苦荞芽中总黄酮和芦丁含量最高,与对照相比分别提高了30.1%、40.9%,蓝光处理其次。同时,经光处理后,苦荞芽中黄酮类物质代谢的关键酶苯丙氨酸解氨酶（PAL）和察尔酮异构酶（CHI）活性均显著升高,与总黄酮和芦丁含量呈现正相关。此外,苦荞萌发过程中,光处理可增加苦荞芽对DPPH自由基的清除率,最高可达35.1%,清除率与总黄酮含量呈正相关,表明光处理可以通过增加苦荞芽中黄酮类物质含量进而提高苦荞的抗氧化活性。      

超声辅助半干法制备马铃薯阳离子淀粉工艺研究

以马铃薯淀粉为原料,2,3-环氧丙基三甲基氯化铵为醚化剂,氢氧化钠为催化剂,采用超声辅助半干法制备了马铃薯阳离子淀粉。在考察单因素实验基础上,利用响应面法对制备马铃薯阳离子淀粉工艺参数进行了优化。结果表明,超声辅助半干法制备马铃薯阳离子淀粉的最佳工艺条件为:超声功率115 W,超声时间40 min,醚化温度60℃,醚化时间3 h。在最优工艺条件下,马铃薯阳离子淀粉的取代度为0.141,反应效率高达85.16%。超声处理能够显著提高阳离子淀粉的取代度和反应效率。      

不同涂膜剂对雷州黑鸭蛋保鲜效果的研究

为研究不同涂膜保鲜剂对雷州黑鸭蛋保鲜效果的影响,采用壳聚糖、白芨为主要原料的中药、壳聚糖和中药复合液及植物油(花生油)四种不同的涂膜剂,对鸭蛋进行涂膜处理。在27℃,湿度65%下贮藏50 d,测定分析评价鸭蛋鲜度的各项指标。结果表明:与对照组相比,复合组与植物油组延缓散黄30 d,显著降低失重率(p<0.01),且植物油组失重率最小(1.61%±0.73%);贮藏30 d,复合组与植物油组可以显著延缓蛋白高度降低(p<0.05);贮藏20 d,植物油组显著延缓了哈氏单位的下降(p<0.05);贮藏30 d,复合组、植物油组抑菌效果极显著高于对照组(p<0.01);整个贮藏期间复合组蛋清p H变化较为平缓。综合各项指标,植物油组保鲜效果最优,其为筛选最适合鸭蛋保鲜涂膜剂提供依据。      

EPLIN,a Putative Tumour Suppressor in Colorectal Cancer,Implications in Drug Resistance

Colorectal cancer is a serious threat to human health. Poor prognosis and frequently reported drug resistance urges research into novel biomarkers and mechanisms to aid in the understanding of the development and progression of colorectal cancer and to optimise therapeutic strategies. In the current study, we investigated the roles of a putative tumour suppressor, EPLIN, in colorectal cancer. Our clinical colorectal cancer cohort and online databases revealed a downregulation of EPLIN in colorectal cancer tissues compared with normal tissues. The reduced expression of EPLIN was associated with poor clinical outcomes of patients. In vitro cellular function assays showed that EPLIN elicited an inhibitory effect on cellular growth, adhesion, migration and invasion. Utilising a protein microarray on protein samples from normal and tumour patient tissues suggested HSP60, Her2 and other signalling events were novel potential interacting partners of EPLIN. It was further revealed that EPLIN and HSP60 were negative regulators of Her2 in colorectal cancer cells. The clinical cohort also demonstrated that expression of HSP60 and Her2 affected clinical outcomes, but most interestingly the combination of EPLIN, HSP60 and Her2 was able to identify patients with the most unfavourable clinical outcome by independently predicting patient overall survival and disease free survival. Furthermore, EPLIN and HSP60 exhibited potential to regulate cellular response to chemotherapeutic and EGFR/Her2 targeted therapeutic agents. In conclusion, EPLIN is an important prognostic factor for patients with colon cancer and reduced EPLIN in CRC contributes to aggressive traits of CRC cells and their responses to chemotherapeutic drugs. Collectively, EPLIN is a pivotal factor for the development and progression of colorectal cancer and has important clinical and therapeutic values in this cancer type.     

SRSF3 and HNRNPH1 Regulate Radiation-Induced Alternative Splicing of Protein Arginine Methyltransferase 5 in Hepatocellular Carcinoma

Protein arginine methyltransferase 5 (PRMT5) is an epigenetic regulator which has been proven to be a potential target for cancer therapy. We observed that PRMT5 underwent alternative splicing (AS) and generated a spliced isoform PRMT5-ISO5 in hepatocellular carcinoma (HCC) patients after radiotherapy. However, the regulatory mechanism and the clinical implications of IR-induced PRMT5 AS are unclear. This work revealed that serine and arginine rich splicing factor 3 (SRSF3) silencing increased PRMT5-ISO5 level, whereas heterogeneous nuclear ribonucleoprotein H 1 (HNRNPH1) silencing reduced it. Then, we found that SRSF3 and HNRNPH1 competitively combined with PRMT5 pre-mRNA located at the region around the 3′- splicing site on intron 2 and the alternative 3′- splicing site on exon 4. IR-induced SRSF3 downregulation led to an elevated level of PRMT5-ISO5, and exogenous expression of PRMT5-ISO5 enhanced cell radiosensitivity. Finally, we confirmed in vivo that IR induced the increased level of PRMT5-ISO5 which in turn enhanced tumor killing and regression, and liver-specific Prmt5 depletion reduced hepatic steatosis and delayed tumor progression of spontaneous HCC. In conclusion, our data uncover the competitive antagonistic interaction of SRSF3 and HNRNPH1 in regulating PRMT5 splicing induced by IR, providing potentially effective radiotherapy by modulating PRMT5 splicing against HCC.     

Inflammatory Mechanism of Brucella Infection in Placental Trophoblast Cells

Brucellosis is a severe zoonotic infectious disease caused by the infection of the Brucella, which is widespread and causes considerable economic losses in underdeveloped areas. Brucella is a facultative intracellular bacteria whose main target cells for infection are macrophages, placental trophoblast cells and dendritic cells. The main clinical signs of Brucella infection in livestock are reproductive disorders and abortion. At present, the pathogenesis of placentitis or abortion caused by Brucella in livestock is not fully understood, and further research on the effect of Brucella on placental development is still necessary. This review will mainly introduce the research progress of Brucella infection of placental trophoblast cells as well as the inflammatory response caused by it, explaining the molecular regulation mechanism of Brucella leading to reproductive system disorders and abortion, and also to provide the scientific basis for revealing the pathogenesis and infection mechanism of Brucella.     

A Novel Antithrombocytopenia Agent,Rhizoma cibotii,Promotes Megakaryopoiesis and Thrombopoiesis through the PI3K/AKT,MEK/ERK,and JAK2/STAT3 Signaling Pathways

Background: Cibotii rhizoma (CR) is a famous traditional Chinese medicine (TCM) used to treat bleeding, rheumatism, lumbago, etc. However, its therapeutic effects and mechanism against thrombocytopenia are still unknown so far. In the study, we investigated the effects of aqueous extracts of Cibotii rhizoma (AECRs) against thrombocytopenia and its molecular mechanism.Methods: Giemsa staining, phalloidin staining, and flow cytometry were performed to measure the effect of AECRs on the megakaryocyte differentiation in K562 and Meg-01 cells. A radiation-induced thrombocytopenia mouse model was constructed to assess the therapeutic actions of AECRs on thrombocytopenia. Network pharmacology and experimental verification were carried out to clarify its mechanism against thrombocytopenia. Results: AECRs promoted megakaryocyte differentiation in K562 and Meg-01 cells and accelerated platelet recovery and megakaryopoiesis with no systemic toxicity in radiation-induced thrombocytopenia mice. The PI3K/AKT, MEK/ERK, and JAK2/STAT3 signaling pathways contributed to AECR-induced megakaryocyte differentiation. The suppression of the above signaling pathways by their inhibitors blocked AERC-induced megakaryocyte differentiation. Conclusions: AECRs can promote megakaryopoiesis and thrombopoiesis through activating PI3K/AKT, MEK/ERK, and JAK2/STAT3 signaling pathways, which has the potential to treat radiation-induced thrombocytopenia in the clinic.     

云南某金矿选矿试验研究

针对云南某金矿石金以细粒、微细粒赋存于石英、黄铁矿的性质,在磨矿细度-74μm75%、T31为活化剂、丁基黄药与A25为捕收剂的条件下,采用一粗一扫两精的工艺流程,获得了金精矿品位79.30g/t,回收率89.45%的较好指标。