全文获取类型
收费全文 | 993篇 |
免费 | 31篇 |
国内免费 | 26篇 |
专业分类
电工技术 | 39篇 |
综合类 | 60篇 |
化学工业 | 54篇 |
金属工艺 | 186篇 |
机械仪表 | 53篇 |
建筑科学 | 26篇 |
矿业工程 | 1篇 |
能源动力 | 13篇 |
轻工业 | 285篇 |
水利工程 | 6篇 |
石油天然气 | 1篇 |
无线电 | 50篇 |
一般工业技术 | 171篇 |
冶金工业 | 23篇 |
原子能技术 | 19篇 |
自动化技术 | 63篇 |
出版年
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 8篇 |
2020年 | 3篇 |
2019年 | 11篇 |
2018年 | 7篇 |
2017年 | 10篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 37篇 |
2013年 | 145篇 |
2012年 | 75篇 |
2011年 | 102篇 |
2010年 | 58篇 |
2009年 | 82篇 |
2008年 | 47篇 |
2007年 | 35篇 |
2006年 | 51篇 |
2005年 | 21篇 |
2004年 | 30篇 |
2003年 | 17篇 |
2002年 | 13篇 |
2001年 | 6篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 20篇 |
1997年 | 58篇 |
1996年 | 20篇 |
1995年 | 10篇 |
1994年 | 17篇 |
1993年 | 13篇 |
1992年 | 10篇 |
1991年 | 10篇 |
1990年 | 4篇 |
1989年 | 14篇 |
1988年 | 7篇 |
1987年 | 15篇 |
1986年 | 9篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1978年 | 6篇 |
1976年 | 4篇 |
1975年 | 5篇 |
1973年 | 1篇 |
1970年 | 1篇 |
1967年 | 2篇 |
1962年 | 1篇 |
1960年 | 2篇 |
1959年 | 1篇 |
排序方式: 共有1050条查询结果,搜索用时 15 毫秒
101.
High-purity immunoglobulins (Ig), which may be useful for immunologic supplementation of food products, were isolated from Cheddar cheese whey in a one-step process using avidin-biotinylated yolk immunoglobulin (IgY) column chromatography. Yolk antibodies specific to bovine IgG (IgYIgG ) were biotinylated with biotinyl amido-hexanoic acid-N-hydroxy-sulfo-succinimide ester without any notable effect on antigen-binding activity, and coupled to immobilized avidin columns. The resulting avidin-biotinylated IgYIgG columns, with binding capacity of 50–55% (w/w percent ratio of IgG to immobilized IgYIgG ), were used for specific binding of IgG from cheese whey. Elution with a commercially available eluent (Actisep) or 0.1 M glycine-HCl buffer at pH 2.8 yielded IgG with purity of 99% by radial immunodiffusion. 相似文献
102.
YANBIN LI MICHAEL F. SLAVIK CARL L. GRIFFIS JEONG-WEON KIM 《Journal of Food Safety》1993,13(4):241-252
Electrical stimulation was evaluated as a method to kill Salmonella typhimurium in various salt solutions at different concentration . Salmonella typhimurium at 2 × 105 CFU/ml was treated at 22–24C for 60 min in each salt solution using electricity at 10 mA/cm2 current, 1 kHz frequency, and 50% duty cycle. Samples taken at various times were serially diluted, plated on tryptic soy agar and xylose lysine desoxycholate agar, and incubated at 37C for 18–24 h. To detect injured cells, samples were also pre-enriched in buffered peptone water at 37C for 4–5h before being plated. Results indicated all salmonellae were electrically killed at 5 min in NaCl, at 30 min in NaNO3 , and at 45 min in NaC2 H3 O2 at 0.15 and 0.015 M concentrations. Salmonellae were also killed at 45 min in Na3 PO4 and at 60 min in Na2 CO3 at 0.0015 M concentration by electricity in combination with high pH . 相似文献
103.
Solvent extracts were prepared from Manda Enzyme®, one of the fermented health foods, and their activities of radical scavenging and cancer cell growth inhibition were evaluated. Manda Enzyme® was extracted with 55% ethanol, and then fractionated into n‐hexane, chloroform, ethyl acetate, methanol‐soluble and methanol‐insoluble fractions. The antioxidant activities were in the order chloroform > ethyl acetate > other fractions and of each fraction were positively related to the amount of total phenolics and the intensity of brown color. The cancer cell growth inhibitory activities were in the order n‐hexane > chloroform > other fractions. Proliferation of HRT‐18, HCT‐48 and HepG2 human cancer cells was inhibited by the treatment of the n‐hexane fraction of Manda Enzyme® at a concentration of 400 μg/mL to the extent of 75, 89 and 90%, respectively. From these results, it is considered that Manda Enzyme® has chemically different ingredients showing strong antioxidant and anticancer activity in vitro. 相似文献
104.
Canola Phytase: Isolation and Characterization 总被引:1,自引:0,他引:1
Two phytase isoenzymes were isolated from 8-day germinated canola cv Regent. Gel filtration chromatography of an ammonium sulfate fractionated extract on Sephadex G-100 produced one peak with phytase activity. The phytase fraction was separated into two isoenzymes by DEAE-cellulose chromatography. The optimum pH was 4.5–5.0 and 5.0 for the phytase isoenzymes 1 and 2, respectively. Both isoenzymes exhibited maximum activity at 50°C. Km values at pH 5.0 were 0.36 and 0.25 mM for phytase 1 and 2 isoenzymes, respectively, while molecular weight determination showed both fraction were identical with a molecular weight of 70,100 ± 4,000 daltons. 相似文献
105.
In-Soo SON Sang-Pill LEE Jin-Kyung LEE Woo-Cheol KIM Ji-Seon MOON Sangmok LEE Jong-Sup LEE Yong-Bae KIM Geun-Ahn LEE Dong-Su BAE 《中国有色金属学会会刊》2014,(Z1):75-80
研究液压对混合复合铸造Al 3003/Al 4004包覆材料微观组织变化的影响。复合铸造Al 3003/Al 4004包覆材料试样为圆形,由Al 3003(外部)和Al 4004(内部)材料组成。采用液压设备,在温度423 K、挤压比5的条件下制造试样。在挤压之前,Al 4004材料的直径为80 mm,Al 3003材料的厚度为35 mm,在挤压后,2种材料的直径和厚度分别为30 mm和5 mm。研究了2个试样的微观组织和硬度。在挤压之前,复合铸造Al 3003/Al 4004包覆材料横截面的硬度以抛物线的形式向中心增大。然而,在挤压后,Al 4004材料的硬度几乎没有变化。在挤压之前,Al 3003和Al 4004材料的界面存在大量直径超过1 mm的大空洞,这些大空洞随着液压过程的进行而消失。 相似文献
106.
Hyun CHO Seok Min HWANG Jae Bin LEE Dong Hyun KA Tae Wan KIM Bo Seul LEE Ji Young LEE Jung-Il LEE Jeong Ho RYU 《中国有色金属学会会刊》2014,(Z1):134-140
采用微波辐射辅助柠檬酸络合法制备Ho3+/Tm3+/Yb3+共掺杂CaWO4纳米晶升频转换荧光粉。将柠檬酸络合物前驱体在300~700°C热处理3 h。Ho3+/Tm3+/Yb3+共掺杂CaWO4C在400°C时开始结晶,在600°C时结晶完成。经600°C热处理的Ho3+/Tm3+/Yb3+共掺杂CaWO4主要呈球形,且形态均匀。在980 nm的激光激发下,Ho3+/Tm3+/Yb3+共掺杂CaWO4纳米晶出现肉眼可见的明亮的白色升频转换发射,这种现象来自Tm3+的475 nm蓝光发射以及Ho3+的543 nm绿光和651 nm红光发射。通过调整Tm3+和Ho3+的浓度可以控制Ho3+/Tm3+/Yb3+共掺杂CaWO4的CIE色度图从冷到暖白色之间变化。讨论了Tm3+和Ho3+浓度对升频转换光性能的影响以及与激光泵功率相关的影响机制。 相似文献
107.
108.
Abstract. A new hybrid classifier is proposed which utilizes the advantages of the maximum likelihood classifier and the parallelepiped classifier. The new hybrid classifier reduces the processing time more substantially than the conventional one by introducing a lower boundary for each class. The rate of misclassification is comparable to that of maximum likelihood classifier. Performance of the conventional and the new method are compared by an example. 相似文献
109.
110.