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91.
A number of shortcomings have been noted in factorial studies of the Child Nowicki-Strickland Internal–External (CNS–IE) scale. Using different computational procedures, factor analysis of the CNS–IE responses of 2 independent samples of British schoolchildren (N?=?188; mean age 14.85 yrs) produced a factor that correlated significantly better with socialization than the total scale score. Results are discussed in terms of their implications for the factorial validity of the CNS–IE scale and the utility of locus of control subscales. (15 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
92.
Casey PG Butler D Gardiner GE Tangney M Simpson P Lawlor PG Stanton C Ross RP Hill C Fitzgerald GF 《Journal of food protection》2004,67(12):2797-2800
Salmonella carriage in pigs represents a serious health problem that undoubtedly contributes to the spread of human disease. Thus, the efficient and reliable testing of farm animals for bacteria such as Salmonella is an important aspect of any efficient control strategy. Serological analysis of 15 meat juice samples detected antibodies against Salmonella in some. but not all, of the animals identified bacteriologically as harboring the pathogen, indicating a lack of correlation between the bacteriological and serological methods used for Salmonella detection. The results suggest that testing by enzyme-linked immunosorbent assay is appropriate at the herd level, with culture methods preferable for individual animal analysis. A novel culture protocol detected Salmonella in the cecal contents of 15 pigs, whereas a method based on the European Standard identified only 9 pigs as being Salmonella-positive. During the study, an unusual finding was the relatively high incidence of Salmonella London carriage in the pigs tested. 相似文献
93.
A new algorithm for ranking the input features and obtaining the best feature subset is developed and illustrated in this paper. The asymptotic formula for mutual information and the expectation maximisation (EM) algorithm are used to developing the feature selection algorithm in this paper. We not only consider the dependence between the features and the class, but also measure the dependence among the features. Even for noisy data, this algorithm still works well. An empirical study is carried out in order to compare the proposed algorithm with the current existing algorithms. The proposed algorithm is illustrated by application to a variety of problems. 相似文献
94.
Mass spectrometry (MS)-based techniques have enormous potential for kinetic studies on enzyme-catalyzed processes. In particular, the use of electrospray ionization (ESI) MS for steady-state measurements is well established. However, there are very few reports of MS-based studies in the pre-steady-state regime, because it is difficult to achieve the time resolution required for this type of experiment. We have recently developed a capillary mixer with adjustable reaction chamber volume for kinetic studies by ESI-MS with millisecond time resolution (Wilson, D. J.; Konermann, L. Anal. Chem. 2003, 75, 6408-6414). Data can be acquired in kinetic mode, where the concentrations of selected reactive species are monitored as a function of time, or in spectral mode, where entire mass spectra are obtained for selected reaction times. Here, we describe the application of this technique to study the kinetics of enzyme reactions. The hydrolysis of p-nitrophenyl acetate by chymotrypsin was chosen as a simple chromophoric model system. On-line addition of a "makeup solvent" immediately prior to ionization allowed the pre-steady-state accumulation of acetylated chymotrypsin to be monitored. The rate constant for acetylation, as well as the dissociation constant of the enzyme-substrate complex obtained from these data, is in excellent agreement with results obtained by conventional stopped-flow methods. Bradykinin was chosen to illustrate the performance of the ESI-MS-based method with a nonchromophoric substrate. In this case, the unfavorable rate constant ratio for acylation and deacylation of the enzyme precluded measurements in the pre-steady-state regime. Steady-state experiments were carried out to determine the turnover number and the Michaelis constant for bradykinin. The methodologies used in this work open a wide range of possibilities for future ESI-MS-based kinetic assays in enzymology. 相似文献
95.
Derek C. Oppen 《Theoretical computer science》1980,12(3):291-302
We restrict our attention to decidable quantifier-free theories, such as the quantifier-free theory of integers under addition, the quantifier-free theory of arrays under storing and selecting, or the quantifier-free theory of list structure under cons, car and cdr. We consider combinations of such theories: theories whose sets of symbols are the union of the sets of the symbols of the individual theories and whose set of axioms is the union of the sets of axioms of the individual theories. We give a general technique for determining the complexity of decidable combinations of theories, and show, for example, that the satisfiability problem for the quantifier-free theory of integers, arrays, list structure and uninterpreted function symbols under +, ≤, store, select, cons, car and cdr is NP-complete. We next consider the complexity of the satisfiability problem for formulas already in disjunctive normal form: why some combinations of theories admit deterministic polynomial time decision procedures while for others the problem is NP-hard. Our analysis hinges on the question of whether the theories being combined are convex; that is, whether any conjunction of literals in the theory can entail a proper disjunction of equalities between variables. This leads to a discussion of the role that case analysis plays in deciding combinations of theories. 相似文献
96.
The Likens-Nickerson concurrent steam distillation-solvent extraction apparatus was used to obtain an essence from malt extract. The essence was submitted to combined gas chromatography-mass spectrometry (g.c.-m.s.), to examination by sulphurspecific and nitrogen-specific detectors, and to sensory evaluation of the components eluted from gas chromatographic capillary columns. Some 33 compounds were identified by direct examination by g.c.-m.s., another 14 by trapping and re-injection, which provides an efficient method of concentration, and a further six, which had not been previously identified due to interference from the solvent peak, by the use of charcoal adsorption traps. Results indicate that several compounds need to be combined to simulate malt flavour. The odour contributions of several components are discussed. 相似文献
97.
Coherent anti-Stokes Raman spectroscopy (CARS) is a nonlinear optical wave mixing process that is used in gas-phase systems to determine the energy distribution of the probed species (usually N2) and, through a fitting procedure, the temperature giving rise to it. CARS signal strengths are maximized when the phase matching condition is met. Because gases are generally non-dispersive, this phase matching condition can be found geometrically as a function of the crossing angles between the CARS beams and their wavelengths. In addition, perfect phase matching in non-dispersive media occurs automatically for collinear beams. To improve spatial resolution, however, intersecting the laser beams is desirable. Being a third-order process, phase matching for CARS in gases typically requires three input laser beams. This paper discusses and demonstrates the issues of phase matching for CARS when the medium is dispersive, and the ability for CARS phase matching to occur with only two crossed laser beams (one pump and one probe). This two-beam X-CARS in dispersive media can be used as an alignment tool for gas-phase CARS and may be relevant as a simpler diagnostic in high-pressure environments. The paper also discusses the effects of non-ideal phase matching in dispersive and non-dispersive media. 相似文献
98.
A nanometre-scale pore in a solid-state membrane provides a new way of electronically probing the structure of single linear polymers, including those of biological interest in their native environments. Previous work with biological protein pores wide enough to let through and sense single-stranded DNA molecules demonstrates the power of using nanopores, but many future tasks and applications call for a robust solid-state pore whose nanometre-scale dimensions and properties may be selected, as one selects the lenses of a microscope. Here we demonstrate a solid-state nanopore microscope capable of observing individual molecules of double-stranded DNA and their folding behaviour. We discuss extensions of the nanopore microscope concept to alternative probing mechanisms and applications, including the study of molecular structure and sequencing. 相似文献
99.
In this paper, the design and development of a physical and virtual environment to support assembly of micron sized devices
is discussed. Micro assembly involves handling and assembling of micron sized parts (10−6 m). As manual assembly of micron-sized parts is difficult and tedious, the design of computer assisted micro-assembly techniques
becomes crucial. An Integrated Physical and Virtual Reality (VR) environment has been created to aid in the assembly of such
micro devices, which supports the establishment of an interface between a virtual and physical micro assembly work cell. The
overall architecture and the various modules implemented in this environment are outlined in this paper. 相似文献
100.
The neural mechanisms contributing to the arousal-eliciting actions of smoking and nicotine involve multiple neurotransmitter systems. The current study examined the role of opioid neurotransmission in modulating the neuroelectric- and mood-activating response to acute nicotine administration in overnight tobacco-deprived smokers. In a randomized, double-blind, placebo-controlled design involving 18 (10 male, 8 female) overnight tobacco-abstinent smokers, spectrally analyzed electroencephalographic (EEG) activity and subjective reports of mood, euphoria, and smoking withdrawal were assessed in response to nicotine gum (4 mg) after pretreatment with placebo or with 50 mg of the opioid antagonist naltrexone. In addition to reducing withdrawal symptoms and increasing euphoria ratings, as well as subjective alertness in male participants, nicotine induced an EEG arousal response consisting of diffuse slow wave (delta, theta) amplitude reductions, frontal fast alpha wave amplitude increments, and elevations in beta wave amplitude, which were greater in female than in male smokers. Naltrexone attenuated the alerting and euphoric actions of nicotine but did not affect nicotine's ameliorating effects on withdrawal symptoms. Nicotine-induced frontal reductions in delta and global reductions in theta were prevented by naltrexone pretreatment, as were increases in anterior recordings of relative fast alpha. These findings suggest that the opioid system is involved in nicotine-induced subjective and neuroelectric arousal and implicate opioid-cholinergic interactions in the elicitation of these arousal responses to nicotine. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献