Preparation of immunomagnetic beads coupled with a rhodamine hydrazine immunosensor for the detection of Mycobacterium avium subspecies paratuberculosis in bovine feces,milk, and colostrum

The aim of this study was to develop and evaluate a method for detecting Mycobacterium avium ssp. paratuberculosis (MAP) bacteria in bovine fecal, milk, and colostrum samples using immunomagnetic beads (IMB) and a rhodamine hydrazone immunosensor. Immunomagnetic beads were prepared by using purified antibodies from hyperimmunized sera that were coupled to Fe nanoparticles with diethylene triamine pentaacetic acid (DTPA) or ethyl (dimethyl aminopropyl) carbodiimide (EDC)-N-hydroxy succinimide (NHS) as linkers. Rhodamine hydrazone particles were synthesized and coupled to IgY anti-MAP antibodies using DTPA or EDC-NHS linkers. Separation efficiency of the IMB was tested on bovine fecal, milk, and colostrum samples experimentally contaminated with MAP. The studied methods were evaluated on their ability to detect MAP and separate bacteria in complex mediums. The ELISA results indicated 95% efficacy in antibody coupling to IMB, with the DTPA-IMB method being more efficient than the EDC-NHS-IMB method. By using the DTPA-IMB method, MAP bacteria were successfully recovered from fecal, milk, and colostrum samples. The DTPA-IMB method used in combination with the rhodamine hydrazone immunosensor had a limit of detection equal to 30 and 30,000 MAP cells/mL using chromogenic and fluorescent properties, respectively. Combining the DTPA-IMB separation method with the rhodamine hydrazone immunosensor provides a fast, sensitive, and cost-beneficial method for detecting MAP in bovine feces, milk, and colostrum.     

Knowledge mapping of the Iranian nanoscience and technology: a text mining approach

Nanoscience and technology (NST) is a relatively new interdisciplinary scientific domain, and scholars from a broad range of different disciplines are contributing to it. However, there is an ambiguity in its structure and in the extent of multidisciplinary scientific collaboration of NST. This paper investigates the multidisciplinary patterns of Iranian research in NST based on a selection of 1,120 ISI??indexed articles published during 1974?C2007. Using text mining techniques, 96 terms were identified as the main terms of the Iranian publications in NST. Then the scientific structure of the Iranian NST was mapped through multidimensional scaling, based upon the co-occurrence of the main terms in the academic publications. The results showed that the NST domain in Iranian publications has a multidisciplinary structure which is composed of different fields, such as pure physics, analytical chemistry, chemistry physics, material science and engineering, polymer science, biochemistry and new emerging topics.     

Tokamak edge plasma rotation in the presence of the biased electrode

Electrode biasing system was designed, constructed, and installed on the IR-T1 tokamak, and then biasing experiments were carried out. Also, using a Mach probes the effects of radial electric field (produced by biased electrode) on the poloidal and toroidal components of the edge plasma velocity were investigated. The results showed an increase in both toroidal and poloidal components of the edge plasma velocity during biasing regime. Results compared and discussed. During positive biasing, increased E_r tends to slow the poloidal rotation in the electron diamagnetic drift direction, i.e., to speed up rotation in the ion diamagnetic drift direction. An increased toroidal rotation velocity has the opposite effect on the poloidal rotation.     

Cylindrical functionally graded shell model based on the first order shear deformation nonlocal strain gradient elasticity theory

Microsystem Technologies - In this article, a cylindrical functionally graded shell model is developed in the framework of nonlocal strain gradient theory for the first time. For this purpose, the...     

Hot Corrosion Behavior and Microstructural Change of Al-Gradient CoNiCrAlYSi Coatings, Produced by LVPS and Diffusional Processes

Conventional and gradient CoNiCrAlYSi coatings were produced by using low vacuum plasma spray and an additional step of diffusional over aluminizing (pack cementation) techniques on an Inconel-738 substrate. Hot corrosion of these coatings was investigated using Na₂SO₄–20wt%NaVO₃ molten salt at 880?°C for 800?h. Hot corrosion rate was determined by measuring the weight gain of the specimens at regular intervals for a duration of 20?h. X-ray diffraction, field emission scanning electron microscopy and electron probe micro analysis techniques were used to determine the nature of phases, investigation of the thermally grown oxide, examination of the surface attack and determination of the elemental distribution. The gradient coating showed better performance by re-healing alumina scale due to its possession of more β phase as Al reservoir. Results indicated that pack cementation process caused an increase in amount of aluminum-rich β phase and better hot corrosion properties of gradient coatings owing to the Al enrichment in the outer layer and rapid formation of protective oxide on the surface.     

Adaptive Fuzzy Fault Tolerant Control Using Dynamic Sliding Mode

International Journal of Control, Automation and Systems - In this paper, an actuator fault tolerant control (FTC) has been designed using dynamic sliding mode control (DSMC) for single input...     

Green synthesis and characterization of RGO/Cu nanocomposites as photocatalytic degradation of organic pollutants in waste-water

Recently, nanocomposite photocatalysts based on semiconductors have attracted much attention due to their suitable bandgap. Combination of tow of several semiconductors can slow down the electron-hole recombination. In this regard, we have depicted an eco-friendly and green fabrication technique to synthesize RGO/Cu nanocomposite by the reduction of graphene oxide and Cu²⁺ ion utilizing spearmint extract as a reductant and capping agent. The sample was identified by FTIR, XRD, FESEM, EDS, HRTEM, and CV. The results of photocatalytic performance revealed that RGO/Cu is an efficient catalyst for degrading organic pollutants. This compound can eliminate Rhodamine B (RhB) and Methylene blue (MB) 91.0% and 72.0%, respectively.