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101.
Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide‐Modified Quantitative PCR 下载免费PDF全文
Dr. Yu Luo Hannah L. Bolt Dr. Gabriela A. Eggimann Prof. Dr. Danny F. McAuley Dr. Ronan McMullan Dr. Tanya Curran Dr. Mei Zhou Professor Colin A. B. Jahoda Dr. Steven L. Cobb Dr. Fionnuala T. Lundy 《Chembiochem : a European journal of chemical biology》2017,18(1):111-118
Biofilms containing Candida albicans are responsible for a wide variety of clinical infections. The protective effects of the biofilm matrix, the low metabolic activity of microorganisms within a biofilm and their high mutation rate, significantly enhance the resistance of biofilms to conventional antimicrobial treatments. Peptoids are peptide‐mimics that share many features of host defence antimicrobial peptides but have increased resistance to proteases and therefore have better stability in vivo. The activity of a library of peptoids was tested against monospecies and polymicrobial bacterial/fungal biofilms. Selected peptoids showed significant bactericidal and fungicidal activity against the polymicrobial biofilms. This coupled with low cytotoxicity suggests that peptoids could offer a new option for the treatment of clinically relevant polymicrobial infections. 相似文献
102.
T. Leslie Youd Hannah W. Bartholomew Jamison H. Steidl 《Canadian Metallurgical Quarterly》2008,134(3):397-400
Automatic trip hammers have advantages for standard penetration test (SPT) of consistent drop height and low friction loss during hammer fall. These advantages, however, generate high energy transfer ratios (ER), typically about 90%. This efficiency causes lower sensitivity and higher energy correction coefficients, CE. To reduce ER and CE and to increase the sensitivity of SPT conducted at the Wildlife Liquefaction Array (WLA) and the Garner Valley Downhole Array, instrumented Network for Earthquake Engineering Simulation sites, a 127?mm (5.00?in.) long sleeve was placed in the hammer mechanism to reduce the drop height from 762?mm (30?in.) to 635?mm (25in.). To calibrate the energy for these drop heights, measurements were made for a series of SPT tests in Borehole X2 at WLA on November 21, 2003. For these SPT, sleeves were inserted with lengths of 50?mm (2?in.), 127?mm (5?in.) 177?mm (7?in.), and no sleeve. Resulting drop heights were 762?mm (30?in.), 711?mm (28?in.), 635?mm (25?in.), and 584?mm (23?in.). Results indicate that: (1) ER increases with rod length as expected; (2) corrections for rod length, CR, increased with rod length in accordance with CR published in 2001 by Youd et al.; and (3) for lengths greater than 6?m, ER increased approximately linearly with drop height. Average ER30 [ER based on a 762?mm (30?in.) drop height] were 43% for a 584?mm (23?in.) drop, 60% for a 635?mm (25?in.) drop, 84% for 711?mm (28?in.) drop, and 89% for a 762?mm (30?in.) drop. 相似文献
103.
Hannah McLachlan 《Chemical Engineering Communications》2016,203(9):1189-1197
Confidential industrial trials showed that higher purity crystals were produced in an oscillatory baffled crystallizer (OBC) compared to a stirred tank crystallizer (STC) when operating at similar conditions. This trend is verified in this work using urea as the test compound that is different from that was employed in industrial tests. We have observed that a higher supersaturation level at nucleation coupled with a lower nucleation temperature was measured in the STC at all investigated conditions compared to that in the OBC. This led to a higher nucleation rate in the STC, consequently producing smaller crystals. Crystal size distributions and imaging analyses suggest that these smaller crystals were more likely to form agglomerates in the STC, trapping either mother liquor or impurity or both and leading to the lower purity observed when compared to that in the OBC. 相似文献
104.
We demonstrate vertically aligned epitaxial GaAs nanowires of excellent crystallographic quality and optimal shape, grown by Au nanoparticle-catalyzed metalorganic chemical vapor deposition. This is achieved by a two-temperature growth procedure, consisting of a brief initial high-temperature growth step followed by prolonged growth at a lower temperature. The initial high-temperature step is essential for obtaining straight, vertically aligned epitaxial nanowires on the (111)B GaAs substrate. The lower temperature employed for subsequent growth imparts superior nanowire morphology and crystallographic quality by minimizing radial growth and eliminating twinning defects. Photoluminescence measurements confirm the excellent optical quality of these two-temperature grown nanowires. Two mechanisms are proposed to explain the success of this two-temperature growth process, one involving Au nanoparticle-GaAs interface conditions and the other involving melting-solidification temperature hysteresis of the Au-Ga nanoparticle alloy. 相似文献
105.
106.
Jan Hahn Manuela Moritz Hannah Voß Penelope Pelczar Samuel Huber Hartmut Schlüter 《International journal of molecular sciences》2021,22(19)
It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient for sampling and homogenizing biological tissue. In this study we utilized a tunable nanosecond infrared laser (NIRL) for tissue sampling and homogenization with subsequent liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis for mass spectrometric proteomics. For the first time, laser sampling was performed with murine spleen and colon tissue. An ablation volume of 1.1 × 1.1 × 0.4 mm³ (approximately 0.5 µL) was determined with optical coherence tomography (OCT). The results of bottom-up proteomics revealed proteins with significant abundance differences for both tissue types, which are in accordance with the corresponding data of the Human Protein Atlas. The results demonstrate that tissue sampling and homogenization of small tissue volumes less than 1 µL for subsequent mass spectrometric proteomics is feasible with a NIRL. 相似文献
107.
M P Christian C Grainger B J Sutherland J J Mayes M C Hannah B Kefford 《The Journal of dairy research》1999,66(3):341-355
The effects of supplementing cows' diets with protein and energy on milk composition and the composition and yield of Cheddar cheese were investigated. This research addresses the problems of seasonal reduction in the capacity of cheese curds to expel moisture as observed in parts of south-eastern Australia. Milk was collected from cows offered a basal diet of silage and hay supplemented with different sources and levels of dietary protein and energy. The protein supplements were sunflower, canola, cottonseed meal and lupin, and the energy supplements were maize grain, oats, wheat and barely. This milk was used to manufacture Cheddar cheese on a pilot scale. Cheese moisture content was dependent on the source and level of dietary protein and energy. Milk from cows offered the lupin protein supplements and wheat energy supplements consistently produced cheese with a lower moisture content and moisture in fat-free matter. Milk from these supplemented diets had increased casein concentrations and higher proportions of alpha S2-casein than milk from the poor quality control diet. Cheese yield was directly related to the total casein concentration of milk, but was not influenced by differences in casein composition. Supplementing the cows' diets increased the inorganic P, Mg and Ca concentrations in milk. A low inorganic P concentration in milk from cows offered the control diet was caused by a low intake of dietary P. These findings showed that changes in the mineral and casein composition of milk, associated with diet, could influence the composition of Cheddar cheese. 相似文献
108.
Freshwater mussels are often relocated from existing beds for both conservation and management reasons. In this study, we empirically tested whether the habitat type at the destination site was important in predicting the success of mussel relocation. In 1993, four species of freshwater mussels were relocated in the Apalachicola River in Florida, into three distinct habitat types: stable sand, limestone/sand and cobble. The conditional probability of survival of relocated mussels varied by species and habitat. Two species were considered habitat specialists, one species was considered a habitat generalist and recovery rates for the fourth species were too low to assess habitat preferences. We show empirically that microhabitat is important in the survival of relocated mussels and that the habitat-specific criteria for relocation is species specific. These results suggest that survival of relocated mussels can be enhanced if species-specific site selection criteria are developed using quantitative information. © 1997 John Wiley & Sons, Ltd. 相似文献
109.
110.
We propose a new method that treats visible human behaviour at the level of navigational strategies. By inferring intentions in terms of known goals, it is possible to explain the behaviour of people moving around within the field of view of a video camera. The approach presented here incorporates models of navigation from within psychology which are both simple and conceptually plausible, whilst providing good results in an event-detection application. The output is in the form of statements involving goals, such as “Agent 25 went to exit 8 via sub-goals 34 and 21” for a given navigational strategy, an image representing the path through the scene, and an overall score for each trajectory. The central algorithm generates all plausible paths through the scene to known goal sites and then compares each path to the agent's actual trajectory thus finding the most likely explanation for their behaviour. Two navigational strategies are examined, shortest path and simplest path. Experimental results are presented for an outdoor car-park and an indoor foyer scene, and our method is found to produce psychologically plausible explanations in the majority of cases. We propose a novel approach to determining the effectiveness of event detection systems, and evaluate the method presented here against this new ground truth. This evaluation method uses human observers to judge the behaviour shown in various video clips, then uses these judgements in correlation with those of the software. We compare the method with a standard machine learning approach based on nearest neighbour. Finally we consider the application of such a system in a binary event-detection or behaviour filtering system. 相似文献