Juice of New citrus hybrids (Citrus clementina Hort. ex Tan.×C. sinensis L. Osbeck) as a source of natural antioxidants

Fruit juices of pigmented and non-pigmented new citrus hybrids obtained by crossing clementine cv. Oroval with different cultivars of blood oranges were analysed to determine parameters related to fruit quality (total soluble solids titratable acidity, pH), and the content of ascorbic acid, total phenolics, flavanones, anthocyanins and phenolic acids. Antioxidant properties were evaluated by oxygen radical absorbance capacity (ORAC), and inhibition of induced linoleic acid peroxidation (InLAP) assays. The results of this study show that some hybrids with high antioxidant activity owing to considerable polyphenol content may be considered rich sources of phytochemicals. The OTA 9 hybrid was shown to be richest in polyphenols, suggesting that consumption of OTA 9 fruit or juice could be useful in health promotion and a disease-preventing diet. Moreover, the juice of this hybrid could be used as raw material to produce antioxidant ingredients for dietary, pharmaceutical, or cosmetic purposes.     

Recent relative sea-level trends: an attempt to quantify the forcing factors

Local sea-level is affected by a number of forcing factors, which all contribute to the trends observed by tide gauges. Here we use the fingerprints of main factors contributing to secular sea-level trends to construct an initial empirical model that explains best the trends in sea-level as recorded by the large number of coastal tide gauges over the last 50 years. The forcing factors considered include steric changes derived from observations, post-glacial rebound as predicted by geophysical models and mass changes in the Greenland and Antarctic ice sheets as predicted by the static sea-level equation. The approximation of the observed spatial pattern of sea-level trends through a model based on the spatial fingerprints of the main forcing factors fully utilizes the information contents of the available observations and models and allows the interpolation of the sea-level trends between the tide gauges. As a result, we obtain the global picture of sea-level trends due to the forcing factors accounted for in the analysis. Moreover, we derive constraints on the mass changes of the large ice sheets.The empirical models explain about 15% of the variance of the sea-level trends. Nevertheless, the models are correlated with the observations on the level of 0.38+/-0.07, indicating that most of the unexplained variance is due to contributions with small spatial scales. Averaged over the last five decades, the results indicate that the Antarctic and Greenland ice sheets have been melting with an equivalent contribution to global sea-level rise of 0.39+/-0.11 and 0.10+/-0.05 mm yr(-1), respectively. The steric signal derived from observations is clearly identified in the sea-level trends and is found to be at a minimum of 0.2 mm yr(-1), with the most likely value being close to 0.35 mm yr(-1). The global tide gauge network, which covers only a small fraction of the ocean surface, appears to sense an average sea-level rise larger than the global average. Extrapolating the regression models to the global ocean and taking into account the uncertainties in the extrapolation results in a most likely global average of the order of 1.05+/-0.75 mm yr(-1).     

Modeling of fiber-optic fluorescence probes for strongly absorbing samples

The dynamic range of fiber-optic fluorescent probes such as single fibers and fiber bundles is calculated for strongly absorbing samples, such as process liquids, foodstuffs, and lubricants. The model assumes an excitation beam profile based on a Lambertian light source and uses analytical forms of the collection efficiency, followed by an Abel transformation and numerical integration. It is found that the effect of primary absorption of the excitation light and secondary absorption of the fluorescence is profound. For fiber bundles and bifurcated fiber probes, the upper accessible concentration limit is roughly given by the absorption length of the primary and secondary absorption. Fluorescence detectors that are placed at right angles to the excitation beam axis or collinear to the beam axis are equally strongly affected by secondary absorption. A probe in which the same fiber is used for excitation and for collection of the fluorescence emerges as the fiber probe with the largest accessible concentration range.     

Application of the electrochemical microcapillary technique to study intergranular stress corrosion cracking of austenitic stainless steel on the micrometre scale

The electrochemical microcapillary technique was applied for the first time to study the electrochemical dissolution signals from single growing cracks due to stress corrosion cracking of thermally sensitised AISI 304 stainless steel in potassium tetrathionate solution. Potentiostatic current measurements on initiating cracks along grain boundaries were performed. Typical current signals of potentiostatic and potentiodynamic measurements consisted of a series of current peaks showing fast rise and exponential decay, sometimes interrupted by passive phases. The results indicate that stress corrosion crack growth is a discontinuous process of passive and active phases, which might be explained by the film rupture model.     

Preparation and sintering behaviour of a fine grain BaTiO<Subscript>3</Subscript> powder containing 10 mol% BaGeO<Subscript>3</Subscript>

The formation of solid solutions of the type [Ba(HOC₂H₄OH)₄][Ti_1−x Ge _x (OC₂H₄O)₃] as Ba(Ti_1−x /Ge _x )O₃ precursors and the phase evolution during thermal decomposition of [Ba(HOC₂H₄OH)₄][Ti_0.9Ge_0.1(OC₂H₄O)₃] (1) are described herein. The 1,2-ethanediolato complex 1 decomposes above 589 °C to a mixture of BaTiO₃ and BaGeO₃. A heating rate controlled calcination procedure, up to 730 °C, leads to a nm-sized Ba(Ti_0.9/Ge_0.1)O₃ powder (1a) with a specific surface area of S = 16.9 m²/g, whereas a constant heating rate calcination at 1,000 °C for 2 h yields a powder (1b) of S = 3.0 m²/g. The shrinkage and sintering behaviour of the resulting Ba(Ti_0.9/Ge_0.1)O₃ powder compacts in comparison with nm-sized BaTiO₃ powder compacts (2a) has been investigated. A two-step sintering procedure of nm-sized Ba(Ti_0.9/Ge_0.1)O₃ compacts (1a) leads, below 900 °C, to ceramic bodies with a relative density of ≥90%. Furthermore, the cubic ⇆ tetragonal phase transition temperature has been detected by dilatometry, and the temperature dependence of the dielectric constant (relative permittivity) has also been measured.     

Formation of toxic 2-nonyl-p-benzoquinones from α-tertiary 4-nonylphenol isomers during microbial metabolism of technical nonylphenol

In many environmental compartments, microbial degradation of α-quaternary nonylphenols proceeds along an ipso-substitution pathway. It has been reported that technical nonylphenol contains, besides α-quaternary nonylphenols, minor amounts of various α-H, α-methyl substituted tertiary isomers. Here, we show that potentially toxic metabolites of such minor components are formed during ipso-degradation of technical nonylphenol by Sphingobium xenophagum Bayram, a strain isolated from activated sewage sludge. Small but significant amounts of nonylphenols were converted to the corresponding nonylhydroquinones, which in the presence of air oxygen oxidized to the corresponding nonyl-p-benzoquinones-yielding a complex mixture of potentially toxic metabolites. Through reduction with ascorbic acid and subsequent analysis by gas chromatography-mass spectrometry, we were able to characterize this unique metabolic fingerprint and to show that its components originated for the most part from α-tertiary nonylphenol isomers. Furthermore, our results indicate that the metabolites mixture also contained several α, β-dehydrogenated derivatives of nonyl-p-benzoquinones that originated by hydroxylation induced rearrangement, and subsequent ring and side chain oxidation from α-tertiary nonylphenol isomers. We predict that in nonylphenol polluted natural systems, in which microbial ipso-degradation is prominent, 2-alkylquinone metabolites will be produced and will contribute to the overall toxicity of the remaining material.     

Cheers,proost, saúde: Cultural,contextual and psychological factors of wine and beer consumption in Portugal and in the Netherlands

Wine and beer consumption are an integral part of European culture: Southern Europe is associated with wine and Northern Europe is associated with beer. When consumed in moderation, these alcoholic beverages can be part of a balanced and healthy diet. In the 1990s, non-alcoholic beer (NAB), which has no cultural roots, became available in the market. This review identifies determinants for consumption of wine, beer, and NAB, using data on consumption patterns from Portugal and the Netherlands. Since the 1960s the image of Portugal as a wine country declined, whereas the image of the Netherlands as a beer country remained stable. In each country beer is now the most consumed alcoholic beverage and is mainly a men's beverage, whereas wine is the second most consumed and is consumed by both genders. Cultural differences define Portuguese as “outdoors, everyday drinkers”, within a meal context, and Dutch as “at home, weekend drinkers.” Wine is perceived as the healthiest beverage, followed by NAB, and regular beer. Motivation for consumption is related to context: wine for special occasions, beer for informal occasions, and NAB for occasions when alcohol is not convenient. Moderate wine and beer consumption seems to be surrounded by positive emotions.

This review is relevant for public health, for industry market strategies, and identifies opportunities of future research on drinking behaviour.     

A novel and simple approach for assessing the freshness of hazelnuts

Visual inspection and sensory analysis are the only suitable ways to assess the quality of hazelnuts under routine conditions. To obtain a more objective parameter for the freshness, a fast and easy-to-use method was developed. It is based on the well-known test for the germination capacity of seeds where their vitality is determined by use of 2,3,5-triphenyltetrazolium chloride (TTC) which is reduced by flavo enzymes to 1,3,5-triphenylformazane, which appears red. For the determination, 100 hazelnut halves were embedded in TTC containing methyl cellulose gel on a glass plate and kept at 35 °C in the dark. After 6 h, the cut planes of viable nuts were stained red, the cut planes of rotten nuts were yellow or brown, and the colour of dead or mould-infected nuts remained unchanged. The colour pattern was examined either visually by counting the coloured halves or with computer support using image editing software. The ratio of the sum of coloured areas to the total area of the hazelnut cut planes gave a measure of the average degree of viability ("vitality index"). The analysis of several samples of hazelnuts of well-defined age and storage conditions confirmed the efficiency of the method.