全文获取类型
收费全文 | 6859篇 |
免费 | 1篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 34篇 |
金属工艺 | 2篇 |
机械仪表 | 1篇 |
建筑科学 | 1篇 |
能源动力 | 2篇 |
轻工业 | 18篇 |
水利工程 | 2篇 |
石油天然气 | 4篇 |
无线电 | 24篇 |
一般工业技术 | 23篇 |
冶金工业 | 6733篇 |
原子能技术 | 1篇 |
自动化技术 | 15篇 |
出版年
2016年 | 1篇 |
2014年 | 2篇 |
2013年 | 2篇 |
2012年 | 5篇 |
2011年 | 5篇 |
2010年 | 3篇 |
2009年 | 2篇 |
2008年 | 1篇 |
2007年 | 4篇 |
2006年 | 7篇 |
2005年 | 4篇 |
2004年 | 6篇 |
2003年 | 11篇 |
2002年 | 3篇 |
2001年 | 3篇 |
2000年 | 5篇 |
1999年 | 216篇 |
1998年 | 2311篇 |
1997年 | 1282篇 |
1996年 | 808篇 |
1995年 | 424篇 |
1994年 | 343篇 |
1993年 | 417篇 |
1992年 | 38篇 |
1991年 | 47篇 |
1990年 | 53篇 |
1989年 | 50篇 |
1988年 | 55篇 |
1987年 | 58篇 |
1986年 | 55篇 |
1985年 | 37篇 |
1984年 | 2篇 |
1983年 | 22篇 |
1982年 | 24篇 |
1981年 | 31篇 |
1980年 | 62篇 |
1979年 | 4篇 |
1978年 | 13篇 |
1977年 | 139篇 |
1976年 | 279篇 |
1975年 | 12篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1966年 | 3篇 |
1955年 | 3篇 |
1932年 | 1篇 |
1931年 | 1篇 |
1930年 | 1篇 |
排序方式: 共有6861条查询结果,搜索用时 0 毫秒
141.
142.
143.
MJ Telford JW Martin BJ Rock LL Turik JE Hairfield 《Canadian Metallurgical Quarterly》1994,19(3):25-9, 33
Small local chapter groups can present quality cost effective regional seminars. Key components are dedication of members, teamwork, and a sense of direction. The following guidelines and information can provide a framework for planning and coordinating a seminar. 相似文献
144.
E Krementsova MJ Giffin D Pincus TA Baker 《Canadian Metallurgical Quarterly》1998,273(47):31358-31365
Mu transposase is a member of a protein family that includes many transposases and the retroviral integrases. These recombinases catalyze the DNA cleavage and joining reactions essential for transpositional recombination. Here we demonstrate that, consistent with structural predictions, aspartate 336 of Mu transposase is required for catalysis of both DNA cleavage and DNA joining. This residue, although located 55 rather than 35 residues NH2-terminal of the essential glutamate, is undoubtedly the analog of the second aspartate of the Asp-Asp-35-Glu motif found in other family members. The core domain of Mu transposase consists of two subdomains: the NH2-terminal subdomain (IIA) contains the conserved Asp-Asp-Glu motif residues, whereas the smaller COOH-terminal subdomain (IIB) contains a large positively charged region exposed on its surface. To probe the function of domain IIB, we constructed mutant proteins carrying deletion or substitution mutations within this region. The activity of the deletion proteins revealed that domains IIA and IIB can be provided by different subunits in the transposase tetramer. Substitution mutations at two pairs of exposed lysine residues within the positively charged surface of domain IIB render transposase defective in transposition at a reaction step after DNA cleavage but prior to DNA joining. The severity of this defect depends on the structure of the DNA flanking the cleavage site. Thus, these data suggest that domain IIB is involved in manipulating the DNA near the cleavage site and that this function is important during the transition between the DNA cleavage and the DNA joining steps of recombination. 相似文献
145.
G Zoli GR Corazza S Wood R Bartoli G Gasbarrini MJ Farthing 《Canadian Metallurgical Quarterly》1998,43(6):759-762
Relatively little is known about the occurrence of neurocysticercosis in northeastern Brazil. There have been no published reports from the state of Ceará, but a review of the records at the Hospital S?o José in Fortaleza, Brazil identified 119 patients with neurocysticercosis diagnosed between January 1988 and April 1994. Patients came from 43 municipalities in Ceará. Their ages ranged from five to 74 years; the greatest number of cases were in persons 10-40 years of age; 63% were males. Seizures were the presenting complaint in 64% of the patients and headache in 22%. Two patients, each with several hundred intracranial lesions, presented with mental status changes; one was initially given the clinical diagnosis of viral meningoencephalitis. Computed tomography scans showed that 44% of the patients had five or more lesions. Cysts were found throughout the brain. The parietal lobe was the most frequent site of involvement; 85% of patients had one or more lesions there. The brain stem was involved in 8%. There was no consistent association between the severity of the clinical abnormalities and the radiologic findings. Computed tomography of the thighs was done in 10 persons; cysts were identified in nine. 相似文献
146.
147.
MM Ott G Ott H Klinker MJ Trunk T Katzenberger HK Müller-Hermelink 《Canadian Metallurgical Quarterly》1998,22(4):500-506
A 28-year-old man presented with selective immunoglobulin A deficiency and severe diarrhea responding to a gliadin-free diet. Biopsy samples of the small intestine showed dense T-cell infiltrations in the lamina propria and a slight increase of intraepithelial T-lymphocytes. No clonal rearrangement of the T-cell receptor c-beta chain genes was detectable by Southern blotting. Four years later, at the age of 32, the patient was hospitalized again with liver failure, abdominal lymphadenopathy, pancytopenia, and recurrent bacterial infections. Retrospective polymerase chain reaction analysis of formalin-fixed tissues of the intestinal biopsy samples obtained 4 years earlier showed monoclonal T-cell receptor gamma-chain gene rearrangement. Lymphoid cells of the peripheral blood showed an immunophenotype of CD3-positive gamma/delta T cells with a negativity for CD4 and CD8. A clonally rearranged T-cell receptor delta chain gene and a germline configuration of the c-beta chain genes was found by Southern blotting. Cytogenetics showed an abnormal karyotype with unbalanced translocations t(1;5) and t(9;13). The patient died of extensive lung infiltrations by gamma/delta T cells; autopsy showed a peripheral T-cell lymphoma of the gamma/delta type in the enlarged abdominal lymph nodes. This is the first report of an abdominal T-cell lymphoma of the gamma/delta type in a patient with selective immunoglobulin A deficiency. 相似文献
148.
149.
J Balzarini A Karlsson VV Sardana EA Emini MJ Camarasa E De Clercq 《Canadian Metallurgical Quarterly》1994,91(14):6599-6603
Mutant HIV-1 that expresses a Glu138-->Lys substitution in its RT [(E138K)RT] is resistant to the HIV-1-specific RT inhibitor 2',5'-bis-O-(tert-butyldimethylsilyl)-3'-spiro-5"-(4"-amino-1",2"- oxathiole-2",2"-dioxide)pyrimidine (TSAO). However, cell cultures infected with this mutant were completely protected against virus-mediated destruction by micromolar concentrations of the HIV-1-specific RT inhibitors tetrahydroimidazo[4,5,1-jk][1,4]benzodiazepin-2(1H)-one and -thione (TIBO), nevirapine, and bis(heteroaryl)piperazine (BHAP). In contrast, cells infected with a virus mutant that expresses a Tyr181-->Cys substitution in its RT [(Y181C)RT] were not protected by nevirapine and TIBO and were only temporarily protected by BHAP. HIV-1 mutant that emerged under the latter conditions contained a Cys181-->Ile substitution in their RT [(LC181I)RT]. This mutant proved highly resistant to all HIV-1-specific RT inhibitors tested, except for several 1-(2-hydroxyethoxymethyl)-6-(phenylthio)thymine (HEPT) derivatives. When recombinant (C181I)RT was evaluated for susceptibility to the HIV-1-specific RT inhibitors, it was resistant to all inhibitors except the HEPT compounds. Since a (Y181F)RT HIV mutant strain was isolated from cells infected with (Y181C)RT HIV-1 and treated with BHAP, we postulate that the Ile codon was derived from a Cys-->Phe transversion mutation (TGT-->TTT), followed by a Phe-->Ile transversion mutation (TTT-->ATT). 相似文献
150.
The experience of being "in love" was studied in a sample of 186 early to middle adolescent males and 199 early to middle adolescent females. Results indicated that amount of dating experience of adolescents varied widely at each age. Being "in love" co-occurred with a reciprocal on-going relationship about half the time. Boys fell in love earlier and more often than girls, and both genders seemed to employ an increasingly narrow prototypical conception of being "in love". Qualitative analyses suggested that early and middle adolescents are actively reasoning about the nature and meaning of romantic feelings and experiences. Results are interpreted from the standpoint of psychosocial developmental theory, and implications for interventions with adolescents are discussed. 相似文献