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71.
As the genetic and physical mapping stage of the Human Genome Project nears completion, the focus is shifting toward the development of technologies for high-throughput analysis of gene function. Whereas DNA sequencing will enable the assignment of presumed function to a large number of genes in mice and humans, it is clear that the great majority of genes will have to be evaluated in vivo to accurately assess their role in a complex organism. While gene targeting in mouse embryonic stem (ES) cells is the current method of choice for the characterization of gene function in mice, it remains relatively labor intensive and lacks the throughput required for analysis of genome function on a large scale. Alternative methods of efficient mutagenesis will clearly be required for this task. Chromosomal deletions are powerful tools in the genetic analysis of complex genomes, enabling the systematic identification and localization of functional units along defined chromosomal regions. Not only are deletions useful for the identification of genetic functions, but they serve as mapping reagents for existing mutations or traits. While their use has been an essential tool in Drosophila genetics, classical mutagenesis in mice has been logistically impractical for generating deletions. We have previously described an efficient method for generating radiation-induced deletion complexes at defined regions in the genome using ES cells. In this article, we detail the methodological aspects of this technology and describe the applications of chromosomal deletions for characterizing gene function in ways that make optimal use of the information generated by the first stage of the Genome Project. 相似文献
72.
The paper investigates multichannel switching as a promising alternative to traditional single-channel switching where virtual paths established in a switch are between a single input channel and a single output channel. A particular non-blocking condition is derived for flip networks, which is exploited to realize a multichannel switching architecture that supports an arbitrary number of channel groups. The architecture is internally nonblocking and bufferless. Using one flip network recursively a number of times based on the number of channel groups, the resulting architecture becomes efficient in the sense that the cross point complexity is O(N log2 N) for N inputs. Other distinguishing features are the abilities to provide multicasting, superrate switching (i.e., rates that exceed the capacity of a single channel are accommodated), multirate switching (i.e., bit pipes of different rates are supported simultaneously), multiple performance requirements (i.e., services with different performance requirements are treated accordingly), and fair access to all inputs (i.e., no input is systematically discriminated against). In multichannel switching, cells belonging to a single session can traverse multiple channels. Providing the cell sequencing integrity becomes a challenging issue. The architecture proposed in the paper accomplishes the task without employing any cell resequencing mechanism 相似文献
73.
Mating success of large and small flies of Drosophila malerkotliana and D. bipectinata was studied using multiple, male and female choice methods. In multiple choice method the large male mated with large female and small male paired with small female. In female choice method, large male was successful in mating with female irrespective of its size while in male choice method, preferential mating occurred between large male and large female as well as small male and small female. The consequence of such non-random mating in the presence of male rivarly and preferential mating has been discussed. 相似文献
74.
The phylogenetic distribution of transposable families, P, gypsy, hobo, I, and mariner has been analyzed in 33 species of 11 groups of neotropical Drosophila and a Drosophilidae species Zygotrica vittimaculosa, using squash blot and dot blot. Genomic DNA of almost all neotropical species tested hybridized with gypsy probe and some species showed a particularly strong hybridization signal, as D. gaucha, D. virilis, and species of flavopilosa group. The hobo element was restricted to melanogaster group and some strains of D. willistoni. Only D. simulans DNA showed hybridization to mariner probe in all species tested and D. simulans and D. melanogaster showed hybridization with I element probe. P element homologous sequence was present in D. melanogaster and all species and strains of the willistoni and saltans groups tested. The presence of at least one P-homologous sequence was detected in Drosophila mediopunctata. This one was the only P-bearing species of all six tested from the tripunctata group. Four different pairs of primers homologous to segments of the canonical sequence of D. melanogaster's P were used to amplify specific sequences from D. mediopunctata DNA, showing the occurrence of seemingly well-conserved P-homologous sequences. 相似文献
75.
Conservative Approximation–Based Full‐Search Block Matching Algorithm Architecture for QCIF Digital Video Employing Systolic Array Architecture
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This paper presents a power‐efficient hardware realization for a motion estimation technique that is based on the full‐search block matching algorithm (FSBMA). The considered input is the quarter common intermediate format of digital video. The mean of absolute difference (MAD) is the distortion criteria employed for the block matching process. The conventional architecture considered for the hardware realization of FSBMA is that of the shift register–based 2‐D systolic array. For this architecture, a conservative approximation technique is adapted to eliminate unnecessary MAD computations involved in the block matching process. Upon introducing the technique to the conventional architecture, the power and complexity of its implantation is reduced, while the accuracy of the motion vector extracted from the block matching process is preserved. The proposed architecture is verified for its functional specifications. A performance evaluation of the proposed architecture is carried out using parameters such as power, area, operating frequency, and efficiency. 相似文献
76.
An Adaptive Non Reference Anchor Array Framework for Audio Retrieval in Teleconferencing Environment
Karan Nathwani Arpit Shukla Shubham Khunteta Rajesh M. Hegde 《Journal of Signal Processing Systems》2014,74(1):91-102
In this paper, an adaptive framework for audio retrieval in live teleconferencing environments with multiple participants is proposed. The framework uses a non reference anchor array (NRA) to capture the interfering speech sources, in addition to the primary array that captures the speech source of interest (SOI). A linearly constrained-minimum variance (LC-MV) beamformer is used herein such that the signal coming from the look direction is preserved while interferences coming from the non look direction are nulled. Additionally, the reverberant component of the speech acquired by this framework is removed by a novel method that uses the linear prediction (LP) residual cepstrum. This method does not require the computation of the acoustic impulse response (AIR) of the teleconferencing room and hence is computationally efficient. The NRA framework is therefore able to remove correlated noise coming from the direction of the SOI and also dereverberating the noise free signal. The performance of the proposed framework is evaluated by conducting experiments on clean speech acquisition from distant microphone arrays. Experiments on distant speech recognition are also conducted using the TIMIT and MONC databases. Experimental results obtained from the proposed framework indicate a reasonable improvement over correlation, subspace and standard minimum variance beamforming methods. The application of the framework in audio retrieval in a live teleconferencing environment with multiple participants is also discussed. 相似文献
77.
Shreedatta Hegde Ravindrachary Vasachar Rohan Nandeesh Sagar Ismayil Ganesh Sanjeev 《应用聚合物科学杂志》2024,141(19):e55349
In the present study, solid polymer electrolytes (SPEs) based on poly (vinyl alcohol) (PVA) doped with lithium bromide (LiBr) were prepared by solution casting method. Fourier transform infrared spectroscopy results affirm the complexation of LiBr with PVA. X-ray diffraction results exhibit the increase of amorphous nature of the polymer electrolytes, which is also observed in scanning electron microscopy images and atomic force microscopy topographs. Thermogravimetric analysis thermographs endorse the increase of thermal stability of the polymer due to doping. Dielectric studies exhibit non-Debye nature of the polymer electrolytes. Conductivity spectra reveal the maximum ionic conductivity (1.15 × 10−4 S/cm) for 20 wt% LiBr/PVA electrolyte at ambient temperature. Impedance analysis reveals the decrease of ionic relaxation in the polymer electrolytes and the studied transport properties of the electrolyte show that the major contribution to the conduction in this polymer electrolyte is ions. 相似文献
78.
79.
VL Gott 《Canadian Metallurgical Quarterly》1998,47(5):247-252
A high-performance liquid chromatographic (HPLC) method is presented for the simultaneous detection of ubiquinone-9 and 10 in rat tissues such as blood, myocardium, and muscle. After liquid-liquid extraction, the ubiquinones are subsequently analyzed by HPLC with ultraviolet (UV) detection at their maximum absorbance (275 nm). Reference calibration curves in ethanol are used to determine tissular levels of ubiquinones. Because a treatment with HMG-CoA reductase inhibitors is expected to decrease the ubiquinone levels, reference calibration curves are performed to ensure that the ratios (ubiquinone/internal standard) observed in such an experiment could be evaluated directly on a calibration curve. The assay is sensitive (0.0625 microgram/mL), reproducible (4% coefficient of variation for ubiquinone-9 and 6% for ubiquinone-10), and linear up to 20 micrograms/mL (or 100 mg of tissue) for ubiquinone-9 and up to 10 micrograms/mL (or 100 mg of tissue) for ubiquinone-10. The ubiquinone levels in control tissues or blood are within the ranges of those previously reported. 相似文献
80.
VL Tesh 《Canadian Metallurgical Quarterly》1998,6(6):228-233
Many bacterial exotoxins, originally defined by cytopathic effects, may also possess additional biological activities. The capacity of exotoxins to elicit the synthesis and secretion of pro- or anti-inflammatory cytokines may be as important as their direct toxic effects in pathogenesis. One example of such 'molecular crosstalk' occurs between Shiga toxins and the cytokines made in response to these toxins during the development of disease. 相似文献