Electron-beam treatment of aromatic hydrocarbons that can be air-stripped from contaminated groundwater. 2. Gas-phase studies

The electron-beam (EB) degradation of volatile aromatics (benzene, toluene, ethylbenzene, xylenes: BTEX) in groundwater strip gas, which in the present work has been modeled by the introduction of the desired aromatic(s) to a stream of air or another gas, such as oxygen, is initiated essentially by the addition of *OH radicals to the aromatic ring, giving rise to hydroxycyclohexadienyl radicals, which form the corresponding peroxyl radicals upon addition of oxygen. As studied in some detail with benzene as a BTEX representative, various reactions of these lead to numerous oxidation products in a cascade of reactions, including the decomposition of products under the prevailing conditions of high turnover of the initial aromatic. Importantly, hydroxycyclohexadienylperoxyl radical formation is partly reversible, and the reactions of the hydroxycyclohexadienyl radicals, which thus have a significant presence in these systems, must therefore also be taken into consideration. In the gas phase, in contrast to the aqueous phase (see Part 1), the reactions of the hydroxycyclohexadienyl radicals lead to oligomeric products that appear to contribute, in addition to ionic clusters, to nucleation for the aerosols observed. Various nitrated products, among them nitrophenols, are observed when air is used for the stripping. However, these studies did not clear the pilot plant stage, since BTEX degradation using a bioreactor carried out in parallel was so successful that the EB technology was judged to be noncompetitive. As for the latter, expensive equipment consisting of a stripper, the EB machine, and an aerosol precipitator would be required. The condensed aerosols are biorefractory and would require further treatment for detoxification.     

Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence

Visualization of proteins inside acrylamide and other gels usually relies on different staining methods. To omit the protein-staining procedure, we visualized unstained proteins inside acrylamide gels by laser excitation with ultraviolet (UV) light (280 nm, 35 mJ/cm2) and directly detected native UV fluorescence. In one-dimensional gels, a detection limit as low as 1 ng for bovine serum albumin and 5 ng for other proteins with a linear dynamic range (2.7 orders of magnitude) comparable to state of the art fluorescent dyes could be achieved. In addition, the application of this method to 20 microg of a whole cell lysate separated in a two-dimensional gel showed more than 600 spots. Since protein labeling always represents a serious obstacle in protein identification technologies, the working efficiency with our procedure can be considered as a significant improvement for protein visualization and reproducibility in proteomics.     

An integrated microfluidic system for reaction, high-sensitivity detection, and sorting of fluorescent cells and particles

Presented is a novel approach for an integrated micro total analysis system (microTAS) based on a microfluidic on-chip device that supports ultrasensitive confocal detection of fluorescent cells and particles and subsequently allows for their precise sorting in the fluid phase with respect to spectroscopic properties, such as brightness and color. The hybrid silicone elastomer/glass chip first comprises a branched channel system to initiate fluid mixing and to hydrodynamically focus the sample solution down to a thin flow layer, matching the size of the confocal detection volume placed at that position and, thus, providing a high detection efficiency. In the subsequent on-chip module, the dispersed cells or particles can be sorted into two different output channels. The sorting process is realized by a perpendicular deflection stream that can be switched electrokinetically. The performance of the automated sorting routine is demonstrated by precise partition of a mixture of differently colored fluorescent beads. Moreover, the specifically branched channel geometry allows for direct implementation of reaction steps prior to detection and sorting, which is demonstrated by inducing a selective recognition reaction between the fluorescent protein R-phycoerythrin and a mixture of live bacterial cells exhibiting or lacking the respective surface antigens.     

Accent Patterning on Domain-Related Information in Swedish Travel Dialogues

Investigation of travel-domain dialogues reveals travel-agent (System) utterances with intonational contours characterized by late-timed focal accents on given information. These accents occur on content words in utterance-initial position. The accentuation can be assumed to be related to the interactive nature of the dialogue in which the travel agent links back to a domain-related concept introduced by the client (User) and comments on it in an engaged manner. A perception test using constructed human-machine dialogues in which the machine (synthesized) responses vary as to the type of accent pattern on the initial words was developed to test listeners' preference for accent type. Results indicate that i) focal accents on domain-related utterance-initial given concepts are indeed preferred to nonfocal accents and that ii) late-timed focal accents are preferred to early-timed focal accents. These results have implications for the design of the prosody-generating component of human-machine dialogue systems.     

Working models of attachment and reactions to different forms of caregiving from romantic partners.

Inspired by attachment theory, the authors tested a series of theoretically derived predictions about connections between attachment working models (attachment to one's parents assessed by the Adult Attachment Interview; M. Main & R. Goldwyn, 1994) and the effectiveness of specific types of caregiving spontaneously displayed by dating partners during a stressful conflict-resolution discussion. Each partner first completed the Adult Attachment Interview. One week later, each couple was videotaped while they tried to resolve a current problem in their relationship. Trained observers then rated each interaction for the degree to which (a) emotional, instrumental, and physical caregiving behaviors were displayed; (b) care recipients appeared calmed by their partners' caregiving attempts; and (c) each partner appeared distressed during the discussion. Individuals who had more secure representations of their parents were rated as being more calmed if/when their partners provided greater emotional care, especially if they were rated as more distressed. Conversely, individuals who had more insecure (dismissive) representations of their parents reacted more favorably to instrumental caregiving behaviors from their partners, especially if they were more distressed. The broader theoretical implications of these findings are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Remote monitoring of aerosols with ocean colour sensors: then and now

Synoptic coverage of the temporal and spatial variability of aerosol distribution patterns can only be achieved with satellites. Results from the first ocean colour sensor, the Coastal Zone Color Scanner (CZCS), indicate an annual cycle of the major mineral aerosol plumes that is consistent with the published literature. Seasonality and interannual aerosol variability observed with the CZCS agrees well with that found by ground data measurements and other satellite platforms used to monitor aerosols. The successor to the CZCS—the Sea viewing Wide Field of view Sensor (SeaWiFS)—provides estimates of aerosol load and particle size, both on a global scale. Seasonal maps of both of these aerosol optical properties are in accord with well-known distribution patterns and also with independent satellite estimates. These results indicate that ocean colour sensors are capable of monitoring the variability of global aerosol loads and, more recently, with the retrieval of aerosol particle size, they can be used to characterize different aerosol events.     

Terrestrial laser scanning for estimating urban tree volume and carbon content

The acquisition of single-tree-related information is an important task, especially in urban areas where there is an increasing interest in standing carbon stock. Therefore, an easy and robust method was developed to extract the volume, diameter at breast height (DBH) and height of single trees from point clouds of terrestrial laser scanning. For data acquisition, nine trees were scanned from several positions and the resulting high-resolution point clouds (20–60 million points) were analysed by an algorithm based on voxel structure. First, noise reduction was carried out, followed by filling of voxels inside the stem and branches through the intersection of four orthogonal viewing directions. After the elimination of erroneously generated fillings, volume was determined layer-wise for each cross section. For quality assessment, nine deciduous trees were selected, cut, weighed and analysed for wet specific gravity and carbon content in order to provide a control value. The estimated volumes agree with the control value within a range of –5.1% to +14.3%. This is also the case with DBH values; however, heights are systematically underestimated.     

In situ Characterization of SiO2 Nanoparticle Biointeractions Using BrightSilica

By adding a gold core to silica nanoparticles (BrightSilica), silica‐like nanoparticles are generated that, unlike unmodified silica nanoparticles, provide three types of complementary information to investigate the silica nano‐biointeraction inside eukaryotic cells in situ. Firstly, organic molecules in proximity of and penetrating into the silica shell in live cells are monitored by surface‐enhanced Raman scattering (SERS). The SERS data show interaction of the hybrid silica particles with tyrosine, cysteine and phenylalanine side chains of adsorbed proteins. Composition of the biomolecular corona of BrightSilica nanoparticles differs in fibroblast and macrophage cells. Secondly, quantification of the BrightSilica nanoparticles using laser ablation inductively coupled plasma mass spectrometry (LA‐ICP‐MS) micromapping indicates a different interaction of silica nanoparticles compared to gold nanoparticles under the same experimental conditions. Thirdly, the metal cores allow the investigation of particle distribution and interaction in the cellular ultrastructure by cryo nanoscale X‐ray tomography (cryo‐XT). In 3D reconstructions the assumption is confirmed that BrightSilica nanoparticles enter cells by an endocytotic mechanism. The high SERS intensities are explained by the beneficial plasmonic properties due to agglomeration of BrightSilica. The results have implications for the development of multi‐modal qualitative and quantitative characterization in comparative nanotoxicology and bionanotechnology.