Increasing short-chain fatty acid yield during lipase hydrolysis of a butterfat fraction with periodic aqueous extraction

Factors affecting the release of short-chain fatty acids during hydrolysis of a butterfat fraction with a 1,3-positional and short-chain-specific Penicillium roqueforti lipase were investigated. When a short-chain triglyceride fraction was used as substrate, as opposed to whole butterfat, the ratio of desirable flavor short-chain free fatty acids (FFA) to undesirable medium-chain FFA in the FFA fraction increased from 0.75 to 1.80. However, with both substrates, FFA accumulation eventually led to lipase inhibition and limited the total amount of triglyceride hydrolysis. This inhibition phenomenon was principally due to product inhibition. Periodically extracting the FFA with a buffer solution minimized this inhibition phenomenon, thereby significantly increasing lipase activity and the degree of triglyceride hydrolysis. Thus, on-line extraction of FFA in lipase reactors has the potential of greatly increasing system productivity.     

The effect of goal setting on group performance: A meta-analysis.

Updating and extending the work of O'Leary-Kelly, Martocchio, and Frink (1994), with this meta-analysis on goal setting and group performance we show that specific difficult goals yield considerably higher group performance compared with nonspecific goals (d = 0.80 ± 0.35, k = 23 effect sizes). Moderately difficult and easy goals were also associated with performance benefits relative to nonspecific goals, but these effects were smaller. The overall effect size for all group goals was d = 0.56 ± 0.19 (k = 49). Unexpectedly, task interdependence, task complexity, and participation did not moderate the effect of group goals. Our inventory of multilevel goals in interdependent groups indicated that the effect of individual goals in groups on group performance was contingent upon the focus of the goal: “Egocentric” individual goals, aimed at maximizing individual performance, yielded a particularly negative group-performance effect (d = –1.75 ± 0.60, k = 6), whereas “groupcentric” goals, aimed at maximizing the individual contribution to the group's performance, showed a positive effect (d = 1.20 ± 1.03, k = 4). These findings demonstrate that group goals have a robust effect on group performance. Individual goals can also promote group performance but should be used with caution in interdependent groups. Future research might explore the role of multilevel goals for group performance in more detail. The striking lack of recent field studies in organizational settings that emerged from our brief review of trends in group goal-setting research should be taken into account when designing future studies in this domain. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Increasing short-chain fatty acid yield during lipase hydrolysis of a butterfat fraction with periodic aqueous extraction

Factors affecting the release of short-chain fatty acids during hydrolysis of a butterfat fraction with a 1,3-positional and short-chain-specific Penicillium roqueforti lipase were investigated. When a short-chain triglyceride fraction was used as substrate, as opposed to whole butterfat, the ratio of desirable flavor short-chain free fatty acids (FFA) to undesirable medium-chain FFA in the FFA fraction increased from 0.75 to 1.80. However, with both substrates, FFA accumulation eventually led to lipase inhibition and limited the total amount of triglyceride hydrolysis. This inhibition phenomenon was principally due to product inhibition. Periodically extracting the FFA with a buffer solution minimized this inhibition phenomenon, thereby significantly increasing lipase activity and the degree of triglyceride hydrolysis. Thus, on-line extraction of FFA in lipase reactors has the potential of greatly increasing system productivity.     

Biodiversity and population dynamics of microorganisms in a full-scale membrane bioreactor for municipal wastewater treatment

The total, ammonia-oxidizing, and denitrifying Bacteria in a full-scale membrane bioreactor (MBR) were evaluated monthly for over one year. Microbial communities were analyzed by denaturing gradient gel electrophoresis (DGGE) and clone library analysis of the 16S rRNA and ammonia monooxygenase (amoA) and nitrous oxide reductase (nosZ) genes. The community fingerprints obtained were compared to those from a conventional activated sludge (CAS) process running in parallel treating the same domestic wastewater. Distinct DGGE profiles for all three molecular markers were observed between the two treatment systems, indicating the selection of specific bacterial populations by the contrasting environmental and operational conditions. Comparative 16S rRNA sequencing indicated a diverse bacterial community in the MBR, with phylotypes from the α- and β-Proteobacteria and Bacteroidetes dominating the gene library. The vast majority of sequences retrieved were not closely related to classified organisms or displayed relatively low levels of similarity with any known 16S rRNA gene sequences and thus represent organisms that constitute new taxa. Similarly, the majority of the recovered nosZ sequences were novel and only moderately related to known denitrifiers from the α- and β-Proteobacteria. In contrast, analysis of the amoA gene showed a remarkably simple ammonia-oxidizing community with the detected members almost exclusively affiliated with the Nitrosomonas oligotropha lineage. Major shifts in total bacteria and denitrifying community were detected and these were associated with change in the external carbon added for denitrification enhancement. In spite of this, the MBR was able to maintain a stable process performance during that period. These results significantly expand our knowledge of the biodiversity and population dynamics of microorganisms in MBRs for wastewater treatment.     

Membrane bioreactors as core technology for water loop closure in a maltery.

This paper reports on the potential for water reuse in the malting sector. Core unit of a treatment train to close the water loop was a membrane bioreactor (MBR). Three different commercial submerged membranes were compared in terms of their fouling potential in this application. In a second step, MBR permeate was subjected to reverse osmosis (RO) and several oxidation processes. Neither the MBR permeate nor the RO permeate or oxidized water streams showed an adverse effect on malt quality. The worst case scenario was then tested in a closed water loop over several malting cycles at pilot scale and the effect on water and malt quality investigated.     

Identification of socioeconomic groups at increased risk for smoking in European countries: looking beyond educational level.

Educational level is most often used to identify social groups with increased prevalence of smoking. Other indicators of socioeconomic position (SEP) might, however, be equally or even more discriminatory. This study examined the extent to which smoking behavior is related to other socioeconomic indicators in addition to educational level. Data were derived from the European Household Panel. We selected data for 45,765 respondents aged 25-60 years from nine European countries. The association between six different SEP indicators and smoking prevalence was examined using prevalence rate ratios (RRs) estimated through log linear regression analyses. In univariate analyses, most selected SEP indicators were associated with smoking. In multivariate analyses, educational level, occupational class, accumulated wealth (measured by household assets), and housing tenure retained independent effects on smoking (RRs about 1.20). The effects observed for activity status and household income were small and insignificant in nearly all populations. In northern Europe, educational level had the greatest predictive value in the younger age groups; occupational class and housing tenure predicted most of smoking prevalence in the older age groups. The results showed a less pronounced and more varied pattern in southern Europe. Our results indicate that smoking prevalence is related not only to educational level but also to occupational class and measures of accumulated wealth (other than income). These measures should be used in addition to educational level to identify groups at increased risk for smoking.     

The effect of a feedback signal in a computer mouse on hovering behaviour, productivity, comfort and usability in a field study

The aim of this study was to determine the effect of a tactile feedback signal on hovering behaviour, productivity, usability and comfort after 1 week of using an experimental mouse. In a randomized controlled trial, a regular computer mouse was compared to a new developed mouse with a tactile, vibrating feedback signal to prevent unnecessary hovering above the computer mouse. According to this study, participants do decrease their hovering behaviour when using a mouse with tactile feedback. Furthermore, the mouse with tactile feedback did not influence productivity. Usability was rated somewhat mixed. The use of a mouse with a tactile vibrating feedback signal seems promising for preventing neck, shoulder and arm complaints. Further research is needed to study long-term effects on (prevention of) neck, shoulder and arm complaints and development of learning effects.     

Testing seasonal and long-term controls of streamwater DOC using empirical and process-based models

Concentrations of dissolved organic carbon (DOC) in surface waters are increasing across Europe and parts of North America. Several mechanisms have been proposed to explain these increases including reductions in acid deposition, change in frequency of winter storms and changes in temperature and precipitation patterns. We used two modelling approaches to identify the mechanisms responsible for changing surface water DOC concentrations. Empirical regression analysis and INCA-C, a process-based model of stream-water DOC, were used to simulate long-term (1986-2003) patterns in stream water DOC concentrations in a small boreal stream. Both modelling approaches successfully simulated seasonal and inter-annual patterns in DOC concentration. In both models, seasonal patterns of DOC concentration were controlled by hydrology and inter-annual patterns were explained by climatic variation. There was a non-linear relationship between warmer summer temperatures and INCA-C predicted DOC. Only the empirical model was able to satisfactorily simulate the observed long-term increase in DOC. The observed long-term trends in DOC are likely to be driven by in-soil processes controlled by SO₄²⁻ and Cl⁻ deposition, and to a lesser extent by temperature-controlled processes. Given the projected changes in climate and deposition, future modelling and experimental research should focus on the possible effects of soil temperature and moisture on organic carbon production, sorption and desorption rates, and chemical controls on organic matter solubility.     

Mercury budget of a small forested boreal catchment in southeast Norway

We present input and output fluxes of total mercury (Hg(tot)) and methyl mercury (MeHg) based on throughfall, litterfall and stream water samples from 2004/2005 from a small forested catchment (Langtjern) in Norway. Hg(tot) input via throughfall and litterfall was estimated to 6.7 and 2.7 microg m(-2) yr(-1), respectively, which is considerably lower than previously reported from other boreal catchments in Scandinavia. A likely cause for the low input flux is the sparseness and low productivity of the forest in the Langtjern catchment, with less atmospheric scavenging and lower litterfall fluxes than previously studied sites. In addition there has been a general decrease in mercury (Hg) in the atmosphere on the northern hemisphere in the last decade. The estimated output flux of Hg(tot) with surface water was 2.5 microg m(-2) yr(-1), which is comparable to what has been reported elsewhere. The ratio of Hg(tot) output flux to input flux was 26%, which is considerably higher than reported from other sites. This illustrates that catchment properties have greater importance for surface water export of Hg than the current atmospheric input. The estimated total soil pool of Hg(tot) in the catchment was 17.4 mg m(-2). This corresponds to roughly 8000 years of the current surface water output flux and 2000 years of the current input flux.