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91.
We present here some measurements of thermal diffusion of some polyelectrolytes in aqueous solutions; in this work the Agar and Turner type of cell was used with its ancillary apparatus, built in this laboratory, developed to improve its precision. Isothermal diffusion coefficients were measured with Agar and Lobo's cell. Heat of transport are calculated and discussed. It is shown that some polyelectrolytes behave as a collection of connected spheres where each monomer unit contributes additively to the total heat of transport, whereas others behave differently. 相似文献
92.
Helena Windemann Urs Müller und Erich Baumgartner 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1973,153(1):17-22
Zusammenfassung Die isoelektrische Fokussierung einer wasserlöslichen Proteinfraktion von Hart- und Weichweizen aus Teigwarenprodukten wird mit der bisher üblichen disk-elektrophoretischen Trennmethode verglichen. Die Elektrofokussierung liefert im Gegensatz zur Disk-Elektrophorese auch bei Anwesenheit von Ei-Proteinen sowie nach vorgängiger Hitzedenaturierung der Proteine zuverlässige und genaue Resultate. Im weiteren wird zur besseren Auftrennung und Charakterisierung der wasserlöslichen Proteinfraktion eine zweidimensionale Trennmethode (isoelektrische Fokussierung und anschließende Elektrophorese) beschrieben.
Isoelectric focusing and two-dimensional separation of watersoluble protein fraction of hard and soft wheat from macaroni
Summary The isoelectric focusing of water-soluble protein fraction of hard and soft wheat from macaroni are compared with the disc electrophoresic separation methods. Electrofocusing produced more trustworthy and exacter results in the presence of egg proteins as well for heat-denaturated proteins. Further is described a better separation and characterisation of a water-soluble protein fraction using a two-dimensional system involving isoelectric focusing in the first dimension followed by electrophoresis in the second.相似文献
93.
Cyclic assembly work is known to imply a high risk for musculoskeletal disorders. To have operators rotate between work tasks is believed to be one way of decreasing this risk, since it is expected to increase variation in mechanical and psychological exposures (physical and mental loads). This assumption was investigated by assessing mechanical exposure variability in three assembly tasks in an electronics assembly plant, each on a separate workstation, as well as in a 'job enlargement' scenario combining all three stations. Five experienced operators worked for 1 h on each station. Data on upper trapezius and forearm extensor muscle activity were obtained by means of electromyography (EMG), and working postures of the head and upper arms were assessed by inclinometry. The cycle-to-cycle variance of parameters representing the three exposure dimensions: level, frequency and duration was estimated using ANOVA algorithms for each workstation separately as well as for a balanced combination of all three. For a particular station, the variability of trapezius EMG activity levels relative to the mean was higher than for extensor EMG: between-cycles coefficients of variation (CV) about 0.15 and 0.10, respectively. A similar relationship between CV applied to the parameter describing frequency of EMG activity. Except for head inclination levels, the between-cycles CV was larger for posture parameters than for EMG. The between-cycles variance increased up to six fold in the job enlargement scenario, as compared to working at only one station. The difference in mean exposure between workstations was larger for trapezius EMG parameters than for forearm extensor EMG and postures, and hence the effect of job enlargement on exposure variability was more pronounced for the trapezius. For some stations, job enlargement even implied less cycle-to-cycle variability in forearm extensor EMG parameters than working at that station only. Whether the changes in exposure variability associated with job enlargement were sufficient to imply a decreased risk for musculoskeletal disorders is not known. 相似文献
94.
Ontologies: How can They be Built? 总被引:8,自引:1,他引:7
Ontologies are an important component in many areas, such as knowledge management and organization, electronic commerce and information retrieval and extraction. Several methodologies for ontology building have been proposed. In this article, we provide an overview of ontology building. We start by characterizing the ontology building process and its life cycle. We present the most representative methodologies for building ontologies from scratch, and the proposed techniques, guidelines and methods to help in the construction task. We analyze and compare these methodologies. We describe current research issues in ontology reuse. Finally, we discuss the current trends in ontology building and its future challenges, namely, the new issues for building ontologies for the Semantic Web. 相似文献
95.
Screening of biomarkers in rat urine using LC/electrospray ionization-MS and two-way data analysis 总被引:8,自引:0,他引:8
Idborg-Björkman H Edlund PO Kvalheim OM Schuppe-Koistinen I Jacobsson SP 《Analytical chemistry》2003,75(18):4784-4792
Biofluids, like urine, form very complex matrixes containing a large number of potential biomarkers, that is, changes of endogenous metabolites in response to xenobiotic exposure. This paper describes a fast and sensitive method of screening biomarkers in rat urine. Biomarkers for phospholipidosis, induced by an antidepressant drug, were studied. Urine samples from rats exposed to citalopram were analyzed using solid-phase extraction (SPE) and liquid chromatography mass spectrometry (LC/MS) analysis detecting negative ions. A fast iterative method, called Gentle, was used for the automatic curve resolution, and metabolic fingerprints were obtained. After peak alignment principal component analysis (PCA) was performed for pattern recognition, PCA loadings were studied as a means of discovering potential biomarkers. In this study a number of potential biomarkers of phospholipidosis in rats are discussed. They are reported by their retention time and base peak, as their identification is not within the scope of the study. In addition to the fact that it was possible to differentiate control samples from dosed samples, the data were very easy to interpret, and signals from xenobiotic-related substances were easily removed without affecting the endogenous compounds. The proposed method is a complement or an alternative to NMR for metabolomic applications. 相似文献
96.
Keetch CA Hernánndez H Sterling A Baumert M Allen MH Robinson CV 《Analytical chemistry》2003,75(18):4937-4941
Nanoflow electrospray mass spectrometry has been applied previously to investigate noncovalent protein-protein and protein-ligand interactions. Here we evaluate a commercial microchip device for this application. We show that the microchip can be used to obtain mass spectra of the noncovalent tetramer transthyretin. The device showed a 10-fold increase in signal stability compared with a nanoflow capillary and a high level of nozzle-to-nozzle reproducibility. Binding of the natural ligand thyroxine was clearly observed, and a range of small molecules proposed as inhibitors of transthyretin amyloidosis were shown to be effective in stabilizing the tetramer. We propose that measuring the ability of small molecules to stabilize protein complexes using this automated microchip technology will enable high-throughput screening of multi-protein complexes by mass spectrometry. 相似文献
97.
Osana Helena P.; Lacroix Guy L.; Tucker Bradley J.; Idan Einat; Jabbour Guillaume W. 《Canadian Metallurgical Quarterly》2007,99(4):888
This study extended the work of S. Siddiqui, R. F. West, and K. E. Stanovich (1998), who studied the link between general print exposure and syllogistic reasoning. It was hypothesized that exposure to certain text structures that contain well-delineated logical forms, such as popularized scientific texts, would be a better predictor of deductive reasoning skill than general print exposure, which is not sensitive to the quality of an individual's reading activity. Furthermore, it was predicted that the ability to generate explanatory bridging inferences while reading would also be predictive of syllogistic reasoning. Undergraduate students (N = 112) were tested for vocabulary, nonverbal cognitive ability, exposure to general print, exposure to popularized scientific literature, and the ability to comprehend texts distinguished by the number of inferences that must be generated to support comprehension. Hierarchical multiple regression analyses showed that a combined measure of exposure to general and scientific literature was a significant predictor of syllogistic reasoning ability. Additionally, the ability to comprehend high-inference-load texts was related to solving syllogisms that were inconsistent with world knowledge, indicating an overlap in deductive reasoning skill and text comprehension processes. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
98.
João Carlos Prates Eduardo Fritzen Sean W.M. Siqueira Maria Helena L.B. Braz Leila C.V. de Andrade 《Computers in human behavior》2013
The web is nowadays one of the main information sources, and information search is an important area in which many advances have been registered. One approach to improve web search results is to consider contextual information. Usually, information about context has been provided through user logs on previous searches or the monitoring of clicks on first results, but different approaches can be used in specific environments. In a web based learning environment, existing documents and exchanged messages could provide contextual information. So, the main goal of this work is to provide a contextual web search engine based on shared documents and messages posted in a social network used for collaborative learning. Contextual search is provided through query expansion using learning documents (material provided by the teacher) and discussion messages (posts, links and comments that result from the participants’ interactions). A prototype was implemented and used in a learning scenario to acquire the context in a learning community. The proposed approach makes the context acquisition faster and more dynamic as it considers an automatic approach over text processing of documents and discussions. In addition, the results of the query engine with and without the contextual information were compared and the proposed approach using contextual information showed improvements in the precision of the results. 相似文献
99.
We can never emulate their meekness, soft servants of durable material: they live without pretension in complicated relays and electrical circuits. Speed, docility are their strength. One asks: “What is 2 × 2?”—“Are you a machine?” They answer or refuse to answer, depending on what you demand. There are, however, other machines as well, more abstract automatons, bolder and more inaccessible, which eat their tape in mathematical formulae. They imitate in language. In infinite loops, farther and farther back in their retreat towards more subtle algorithms, more recursive functions. They are logical and describe themselves. As when a man with a hand-mirror pressed against his nose in front of a mirror sees in infinite rows the same image multiplied in a shrinking, darkening corridor of glass. It is a Gödel theorem as good as any. He sees infinity, but what he does not see is his face. (From Göran Printz-Påhlson´s poem “The Turing Machine” published in Säg minns du skeppet Refanut? Samlade dikter 1950–1983 (1984) Bonniers, Stockholm). 相似文献
100.
Rafael F.V. Saracchini Jorge Stolfi Helena C.G. Leitão Gary A. Atkinson Melvyn L. Smith 《Computers in Industry》2013
We show that using example-based photometric stereo, it is possible to achieve realistic reconstructions of the human face. The method can handle non-Lambertian reflectance and attached shadows after a simple calibration step. We use spherical harmonics to model and de-noise the illumination functions from images of a reference object with known shape, and a fast grid technique to invert those functions and recover the surface normal for each point of the target object. The depth coordinate is obtained by weighted multi-scale integration of these normals, using an integration weight mask obtained automatically from the images themselves. We have applied these techniques to improve the PhotoFace system of Hansen et al. (2010). 相似文献