On the Estimation of Differential Entropy From Data Located on Embedded Manifolds

Estimation of the differential entropy from observations of a random variable is of great importance for a wide range of signal processing applications such as source coding, pattern recognition, hypothesis testing, and blind source separation. In this paper, we present a method for estimation of the Shannon differential entropy that accounts for embedded manifolds. The method is based on high-rate quantization theory and forms an extension of the classical nearest-neighbor entropy estimator. The estimator is consistent in the mean square sense and an upper bound on the rate of convergence of the estimator is given. Because of the close connection between compression and Shannon entropy, the proposed method has an advantage over methods estimating the Renyi entropy. Through experiments on uniformly distributed data on known manifolds and real-world speech data we show the accuracy and usefulness of our proposed method.     

Comparative robustness study of planar antennas

Is it possible to produce a planar dipole-like antenna with a reduced conductive area without any loss in either robustness or performance? The objective is to reduce the amount of expensive conductor to be used when applying a meshing technique to the printing of antennas. In this context, robustness means that the characteristics are maintained when the antenna is damaged, for example if it is scratched. This is particularly important for radio frequency identification tags in logistic systems. A general antenna robustness evaluation methodology, based on numerical simulations of a large number of randomly damaged antennas, is used for the antenna comparisons. The antenna performance degradation, based on the return loss (S₁₁) at 868 MHz, is monitored for some basic planar antennas. Finally, we show that it is possible to produce robust low-cost antennas using wire replacements for the solid planar antennas and thus, provided that the robustness requirement is moderate, replace the solid antenna with a thin conductor analogue     

A dual-affinity gene fusion system to express small recombinant proteins in a soluble form: expression and characterization of protein A deletion mutants

A novel gene fusion system to express and purify small recombinantproteins in Escherichia coli has been constructed. The conceptallows for affinity purification of soluble gene products bysequential albumin- and Zn²+-affinity chromatography. The dual-affinitysystem is well suited for expression of unstable proteins asonly full-length protein is obtained after purification andproteins gain proteolytic stability in the fusion protein. Herewe show that the dual-affinity approach can be used for theexpression of various unstable derivatives of a single IgG-bindingdomain based on staphylococcal protein A. Analysis of the proteolyticstabilities and the IgG-binding properties of the differentmutant proteins suggest that the model for the structure ofan IgG-binding domain must be re-evaluated.     

The combined effect of rosemary (Rosmarinus officinalis L.) and modified atmosphere packaging as protection against warmed over flavour in cooked minced pork meat

Rosemary (dried leaves ofRosmarinus officinalis L.) added to cooked meat balls (minced pork) was found to retard the formation of warmed over flavour (WOF) during chill storage. The spice was added to pork meat (Longissimus dorsi) balls in an amount acceptable for the product, as evaluated by a sensory panel (0.05% of total weight). After cooking (water bath, internal temperature 80°C), the meat balls were packed using five atmosphere packaging conditions: (i) air; (ii) 5% O₂/95% N₂; (iii) 3% O₂/97% N₂; (iv) 1% O₂/99% N₂; and (v) 100% N₂. During chill storage (5°C), the sensory quality of the reheated meat balls was evaluated by a trained sensory panel, and lipid oxidation was followed by measurement of thiobarbituric acid reactive substances (TBARS). The combination of decreased O₂ atmosphere in the packages and addition of rosemary resulted in a significantly (p<0.05) lower amount of TBARS and a significantly (p<0.05) better sensory score.

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Gemeinsamer Effekt von Rosmarin (Rosmarinus officinalis L.) und Verpackung in modifizierter Atmosphäre auf den Aufwärmegeschmack (WOF) von wärmebehandeltem, gehacktem Schweinefleisch

Zusammenfassung Rosmarin (getrocknete Blätter vonRosmarinus officinalis L.), erwärmten Fleischklößen (gehacktes Schweinefleisch) zugesetzt, hinderte die Entwicklung des Aufwärmgeschmacks (warmed over flavour) während der Kühllagerung. Das Gewürz wurde den aus Schweinefleisch (Longissimus dorsi) hergestellten Fleischklößen in einer für das Produkt akzeptablen Menge zugegeben, beurteilt von einem sensorischen Panel (0.05% des Totalgewichtes). Nach der Wärmebehandlung (Wasserbad, Zentrum-Temperatur 80°C) wurden die Fleischklöße in fünf unterschiedlichen und kontrollierten Atmosphären wie folgt verpackt: (i) Luft; (ii) 5% O₂/95% N₂; (iii) 3% O₂/97% N₂; (iv) 1% O₂/99% N₂ und (v) 100% N₂. Während der Kühllagerung (5°C) wurde die sensorische Qualität der wiedererwärmten Fleischklöße von einem geschulten Panel beurteilt und die Lipid-Oxidation durch Messung der Thiobarbitursäure-reaktiven Substanzen (TBARS) beobachtet. Die Kombination von ermäßigtem Sauerstoffgehalt in den Verpackungen mit der Zugabe von Rosmarin ergab eine signifikant (p<0.05) niedrigere Menge TBARS wie auch signifikant (p<0.05) bessere Punkte in der sensorischen Beurteilung.

     

Clinical profile of a new monoclonal antibody-based immunoassay for tissue polypeptide antigen

Our preliminary evaluation of a new monoclonal antibody-based assay for tissue polypeptide antigen (TPA) has shown it to be clinically equivalent to the polyclonal antibody-based assay for TPA. The new assay (TPA-M) employs three monoclonal antibodies to epitopes on cytokeratins 8, 18 and 19. This multicenter, multinational study included 266 patients with newly diagnosed carcinomas of the lung, breast, large bowel and urinary bladder. TPA values from the two assays were compared with three other cytokeratin markers (TPS, CYFRA 21-1 and TPACyk) and with the established reference markers for these malignancies (CEA and NSE for lung, CA 15-3 for breast, CEA and CA 19-9 for colorectal tumors). Analysis of receiver operating characteristic (ROC) curves in lung, colorectal and bladder cancer showed similar sensitivities for the two assays, ranging from 50% to 80% with a specificity of 95%. In breast cancer all the markers studied showed poor sensitivity. However, TPA determination by either method could discriminate advanced stage (stages III and IV) from early stage disease (stages 0 to II). TPA showed similar discriminating ability in bladder cancer. On the basis of the results obtained in our patient series, it seems that of the cytokeratin markers studied, TPA and TPA-M are the most sensitive and offer a wide range of clinical applications.     

Elasto-hydrodynamic simulation of complex geometries in hydraulic motors

As many other machines, the radial piston hydraulic motor contains a lot of tribological interfaces. One important area is the piston assembly and the journal bearing contact between the piston and cam roller. There exist good models to describe the performance of simpler geometries such as journal bearings, but when put into a system or when having a more complex geometry, the models do not apply very well. To be able to predict the tribological performance of such components, it is important to have a model that is able to include the real geometry and the properties of the system. A simulation model of the piston assembly in Hägglunds Compact hydraulic motor was built using FE software which made it easy to include the complex geometries. The model includes the deformation of the piston. The hydrodynamics is solved by using Reynolds equation. Density/pressure and viscosity/pressure dependency for the oil are included. Simulation results such as friction, hydrodynamic pressure and oil leakage were compared with test results. Good agreement between simulation and tests shows that this kind of model can be a useful tool in the development and optimization of tribological systems.     

Decay of end effects in graphite/epoxy bolted joints

Almost all analyses of large structures neglect local effects due to the application of loads and due to the transfer of loads between structural elements. In recent years, however, the use in aircraft structures of highly anisotropic materials, such as graphite/epoxy, has motivated more detailed studies of the applicability of Saint-Venant's principle.

In this paper in graphite/epoxy is analysed using the finite element method (FEM). The influence of anisotropy and transition from a thicker to a thinner laminate cross-section (ply-drop) is investigated. The characteristic decay length which is large for highly anisotropic materials is lessened by a change in thickness.     

A mathematical model for estimation of sulphide capacities of multi-component slags

The present work presents mathematical model for the estimation of sulphide capacities. The sulphide capacity, C_S is expressed as (1) where ΔG⁰ is the standard Gibbs energy change for the reaction, (2) ξ, being a function of both temperature and composition, is characterized by the system under consideration. By optimizing ξ, the sulphide capacities could be estimated as functions of temperature and composition. The slag composition is described using a modified Temkin approach, where complex polymeric ions are considered as dissociated to simple species. The model has been applied to the binary systems CaO-SiO₂, MnO-SiO₂ as well as the CaO-MnO-SiO₂ ternary system. The results show that reliable predictions of the sulphide capacities can be obtained using the present model.     

Intra- and inter-laboratory reproducibility of estrogen and progesterone receptor enzyme immunoassay in breast cancer cytosol samples--a Swedish multicenter study. Swedish Society of Cancer Study Group

Estrogen and progesterone receptor analysis results were compared within and between six laboratories in Sweden using frozen breast cancer cytosol samples, and the same technique (enzyme immunoassay, Abbott Laboratories). The concordance in receptor status (positive vs. negative) was excellent (98.4% (571/580)). The discordant results were attributable to values near cut-off (n = 4) or outliers (n = 5), the latter probably being due to analytical errors. One laboratory reported significantly higher ER concentrations than the others; thus caution should be observed when comparing absolute values from different centers. For PgR there were similar differences between the laboratories. However, the intra- and inter-laboratory differences were small compared with the overall variability in ER and PgR content between different samples in a large database. The range of the median intra-laboratory coefficient of variation was 11-23% for ER and 12-19% for PgR, indicating that there is room for improvement in the quality of assay performance.