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21.
Lamarque M Tastet C Poncet J Demettre E Jouin P Vial H Dubremetz JF 《Proteomics. Clinical applications》2008,2(9):1361-1374
The Plasmodium falciparum food vacuole (FV) is a lysosome-like organelle where erythrocyte hemoglobin digestion occurs. It is a favorite target in the development of antimalarials. We have used a tandem mass spectrometry approach to investigate the proteome of an FV-enriched fraction and identified 116 proteins. The electron microscopy analysis and the Western blot data showed that the major component of the fraction was the FV and, as expected, the majority of previously known FV markers were recovered. Of particular interest, several proteins involved in vesicle-mediated trafficking were identified, which are likely to play a key role in FV biogenesis and/or FV protein trafficking. Recovery of parasite surface proteins lends support to the cytostomal pathway of hemoglobin ingestion as a FV trafficking route. We have identified 32 proteins described as hypothetical in the databases. This insight into FV protein content provides new clues towards understanding the biological function of this organelle in P. falciparum. 相似文献
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Abstract The purpose of this study is to provide a preliminary framework to observe, analyse and evaluate both activity and learning in virtual communities. So various types of virtual communities will be studied by examining their relationship to socialisation and learning. After a presentation of the main ideas of Wenger's social learning theory, the principal components of the social context of the emergence and evolution of virtual communities will be described. It will show how taking this context into account enables the definition of four principal types of virtual communities: community of interest, goal-oriented community of interest, learners' community and community of practice and describe how the activity of these communities develops according to the goals they set for themselves and to the strategies they adopt to reach them. For each type of virtual community, an attempt will be made to determine the process of negotiation of meaning at the base of learning, and to describe the learning performed in terms of participation and reification processes. 相似文献
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Christophe Brun Pierre‐Henri Elchinger Guillaume Nonglaton Cheikh Tidiane‐Diagne Raluca Tiron Aurélie Thuaire Didier Gasparutto Xavier Baillin 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(33)
Metallic conductive nanowires (NWs) with DNA bundle core are achieved, thanks to an original process relying on double‐stranded DNA alignment and physical vapor deposition (PVD) metallization steps involving a silicon substrate. First, bundles of DNA are suspended with a repeatable process between 2 µm high parallel electrodes with separating gaps ranging from 800 nm to 2 µm. The process consists in the drop deposition of a DNA lambda‐phage solution on the electrodes followed by a naturally evaporation step. The deposition process is controlled by the DNA concentration within the buffer solution, the drop volume, and the electrode hydrophobicity. The suspended bundles are finally metallized with various thicknesses of titanium and gold by a PVD e‐beam evaporation process. The achieved NWs have a width ranging from a few nanometers up to 100 nm. The electrical behavior of the achieved 60 and 80 nm width metallic NWs is shown to be Ohmic and their intrinsic resistance is estimated according to different geometrical models of the NW section area. For the 80 nm width NWs, a resistance of about few ohms is established, opening exploration fields for applications in microelectronics. 相似文献
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Manuel Portugal Ferreira Cláudia Frias Pinto Fernando Ribeiro Serra 《Scientometrics》2014,98(3):1899-1922
Transaction costs theory (TCT) has long been an important conceptual lens for examining International Business (IB) phenomena and perhaps especially relevant for the study of multinational corporations, entry mode choices and location selection. In this paper we examine the extent to which TCT been used and has impacted IB research. Methodologically, we conduct a bibliometric study of the articles published on nine top journals for publishing IB-related research. We use Jean-François Hennart’s research as the key marker for TCT in IB research given that Hennart’s work has been a hallmark in the discipline. On a sample of 377 articles published between 1982 and 2010, and using the works rather than the authors as the unit of analysis, we analyze citations, co-citations and a spatial visualization of the intellectual research themes delved into. Our analyses provide insights on the influence of Hennart but more broadly of TCT on IB research over the past three decades. We conclude that the TCT has a pervasive influence on a large array of IB research and that Hennart’s work is boundary-spanning, connecting several research themes. 相似文献
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WANG Jiang FAUTRELLE Yves REN Zhong-ming LI Xi NGUYEN-THI Henri MANGELINCK-NOEL Nathalie SALLOUM ABOU JAOUDE Georges ZHONG Yun-bo KALDRE Imants BOJAREVICS Andris BULIGINS Leonid 《钢铁研究学报(英文版)》2012,(Z1):381-385
Sn-3wt%Pb alloy was directionally solidified without and with a 0.08T transverse magnetic field(TMF),and real-time recorded by in-situ synchrotron X-ray imaging.Results indicate that TMF shortened the distance from the location of nucleation to the advancing interface,and accelerated the growth rate of the equiaxed crystal,which caused the columnar-to-equiaxed transition(CET)finally.The thermoelectromagnetic convection(TEMC)in front of the interface and around the crystal’s dendritic branch should respond to changes of the distance and the growth rate. 相似文献
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Jonathan Ben-Naim Henri Prade 《Annals of Mathematics and Artificial Intelligence》2012,64(2-3):247-268
In many multi-agent systems, especially in the field of e-commerce, the users have to decide whether they sufficiently trust an agent to achieve a certain goal. To help users to make such decisions, an increasing number of trust systems have been developed. By trust system, we mean a system that gathers information about an agent and evaluates its trustworthiness on the basis of this information. The aim of the present paper is to develop, and analyze from an axiomatic point of view, new trust systems based on intervals. More precisely, we assume that a set of grades describing the past performances of an agent is given. Then, the goal is to construct an interval that summarizes these grades. In our opinion, such an interval gives a good account of the trustworthiness of the agent. In addition, this kind of representation format overcomes certain limitations (at a certain cost) of the approaches that represent trustworthiness by a single number. We establish seven axioms that should be satisfied by a summarizing method. Next, we develop two new methods. The first one is based on the idea that certain concentrations of grades are strong enough to pull the bounds of the summarizing interval towards themselves. The second one represents data in the setting of possibility theory, and then computes lower and upper expected values. Finally, we check that our methods satisfy the axioms introduced before, which provide theoretical justifications for them. 相似文献