Review

Richard Mabey, Home Country London: Century 1990 £14.95

A Tour of the Lakes in Cumberland. John Ruskin's Diary for 1830. James S. Dearden, (Ed.), with an Introduction by Van Akin Burd. Scolar Press, Aldershot, 1990.

The Landscape Vision of Paul Nash, Roger Cardinal. Reaktion Books, London, 1989. £9,95 paperback ISBN 0948462 02 7

M G R Cannell & M D Hooper, The Greenhouse Effect and Terrestrial Ecosystems of the UK London, HMSO, 1990. 56pp. £4.95

Amenity In Action, The Civic Trust Handbook of Community Initiatives compiled & edited by Deirdre Genet, Societies Development Officer, The Civic Trust (1989), (supported by the Shell Better Britain Campaign)

Sue Minter The Greatest Glass House: The rainforests recreated HMSO, 1990. £25.00     

Moment-rotation-temperature curves for semi-rigid joints

Although connections are known to have a very significant effect on the behaviour of steel and composite framed buildings in the event of fire, the cost of high temperature tests on the broad range of connections used in practice means that their influence is not well detailed in current design codes. The paucity of data also limits the effective use of numerical models developed to simulate the behaviour of complete structures at elevated temperature. This research describes a series of elevated temperature tests conducted on beam-to-column connections. This paper presents moment-rotation-temperature curves for a variety of connections.     

Performance comparison of thermal insulated packaging boxes,bags and refrigerants for single‐parcel shipments

A range of packaging solutions exists for products that must be kept within a specific temperature range throughout the supply‐and‐distribution chain. This report summarizes the results of studies conducted over a span of 2 years by the Consortium for Distribution Packaging at Michigan State University. Thermal insulation packaging materials such as expanded polystyrene, polyurethane, corrugated fibreboard, ThermalCor^® and other composite packaging such as thermal insulating bags were studied. Phase change materials such as gel packs were also evaluated. Properties such as R‐value, melting point and heat absorption were examined and are reported. Copyright © 2007 John Wiley & Sons, Ltd.     

Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation

Relevant parameters for non-covalent protein functionalization of carbon nanotubes are explored. Multiwalled carbon nanotubes are carboxylated and functionalized with metalloproteins. Using atomic force microscopy (AFM) we quantitatively determine that coverage with nitrogen-doped multiwalled carbon nanotubes is superior compared to coverage with un-doped multiwalled carbon nanotubes, due to enhanced carboxylation. Conformational analysis using a combination of AFM, antibody binding assays, circular dichroism and UV-visible spectroscopy demonstrates that the metalloproteins retain their native structure when adsorbed to nitrogen-doped multiwalled carbon nanotubes irrespective of their size, charge or folding motif.     

Energy Quantization in Solution‐Processed Layers of Indium Oxide and Their Application in Resonant Tunneling Diodes

The formation of quantized energy states in ultrathin layers of indium oxide (In₂O₃) grown via spin coating and thermally annealed at 200 °C in air is studied. Optical absorption measurements reveal a characteristic widening of the optical band gap with reducing In₂O₃ layer thickness from ≈43 to ≈3 nm in agreement with theoretical predictions for an infinite quantum well. Through sequential deposition of In₂O₃ and gallium oxide (Ga­₂O₃) layers, superlattice‐like structures with controlled dimensionality and spatially varying conduction band characteristics are demonstrated. This simple method is then explored for the fabrication of functional double‐barrier resonant tunneling diodes. Nanoscale current mapping analysis using conductive atomic force microscopy reveals that resonant tunneling is not uniform but localized in specific regions of the apparent device area. The latter observation is attributed to variation in the layer(s) thickness of the In₂O₃ quantum well and/or the Ga₂O₃ barrier layers. Despite the nonidealities, the tremendous potential of solution‐processable oxide semiconductors for the development of quantum effect devices that have so far been demonstrated only via sophisticated growth techniques is demonstrated.     

The immune-endocrine loop during aging: role of growth hormone and insulin-like growth factor-I

Why a primary lymphoid organ such as the thymus involutes during aging remains a fundamental question in immunology. Aging is associated with a decrease in plasma growth hormone (somatotropin) and IGF-I, and this somatopause of aging suggests a connection between the neuroendocrine and immune systems. Several investigators have demonstrated that treatment with either growth hormone or IGF-I restores architecture of the involuted thymus gland by reversing the loss of immature cortical thymocytes and preventing the decline in thymulin synthesis that occurs in old or GH-deficient animals and humans. The proliferation, differentiation and functions of other components of the immune system, including T and B cells, macrophages and neutrophils, also demonstrate age-associated decrements that can be restored by IGF-I. Knowledge of the mechanism by which cytokines and hormones influence hematopoietic cells is critical to improving the health of aged individuals. Our laboratory has recently demonstrated that IGF-I prevents apoptosis in promyeloid cells, which subsequently permits these cells to differentiate into neutrophils. We also demonstrated that IL-4 acts much like IGF-I to promote survival of promyeloid cells and to activate the enzyme phosphatidylinositol 3'-kinase (PI 3-kinase). However, the receptors for IGF-I and IL-4 are completely different, with the intracellular beta chains of the IGF receptor possessing intrinsic tyrosine kinase activity and the alpha and gammac subunit of the heterodimeric IL-4 receptor utilizing the Janus kinase family of nonreceptor protein kinases to tyrosine phosphorylate downstream targets. Both receptors share many of the components of the PI 3-kinase signal transduction pathway, converging at the level of insulin receptor substrate-1 or insulin receptor subtrate-2 (formally known as 4PS, or IL-4 Phosphorylated Substrate). Our investigations with IGF-I and IL-4 suggest that PI 3-kinase inhibits apoptosis by maintaining high levels of the anti-apoptotic protein Bcl-2. The sharing of common activation molecules, despite vastly different protein structures of their receptors, forms a molecular explanation for the possibility of cross talk between IL-4 and IGF-I in regulating many of the events associated with hematopoietic differentiation, proliferation and survival.     

The modern olympic vaulting pole

A vaulting pole is a combined pivot and energy storage device that enables top pole vaulters to reach heights of over 6 m. This paper describes how modern Olympic poles are highly optimised in terms of material selection and geometry. It also describes how modern techniques in composite production are enabling special design features such as tapered wall thickness and initially bent poles.     

Analyses of the effects of cooling and fire spread on steel-framed buildings

An existing computer model capable of predicting the response of three-dimensional semi-rigid steel framed buildings, including continuous floor systems, at elevated temperatures has been extended to include the possibility of extensive strain reversal within the material constitutive relationship. This allows the behaviour in cooling and the effect o f fire spread on steel-framed buildings to be investigated. A number of examples are presented to give an indication of the likely effects of cooling behaviour within the analysis. An initial study has also been made into the structural effects of fire spread from a single ignition point into adjacent bays of a two-dimensional steel building frame. It is shown that some of the distortions caused by the fire are increased by progressive fire spread, as compared with simultaneous burning across the same range of compartments.     

Practical thermal resistance and ice requirement calculations for insulating packages

Several styles of insulating packages were studied, ranging in size from 0.5 to 5 cubic feet and varying in construction from the ordinary expanded polystyrene cooler to various liner‐in‐box arrangements with and without aluminium foil surfaces. Ice‐melt tests were conducted to measure package insulating ability and the results were used to determine the thermal resistance (R‐value). The R‐value was then related to details connected with package construction including wall thickness, number of layers and number of foil surfaces through a simple equation so that it can be estimated for any construction. The system R‐value can then be used to estimate refrigerant requirements and temperature holding times for known shipping environments. Examples are included. Copyright © 1999 John Wiley & Sons, Ltd.     

Synthesis and evaluation of an intercalator-polyamide hairpin designed to target the inverted CCAAT box 2 in the topoisomerase IIalpha promoter

The synthesis and DNA-binding properties of a novel naphthalimide-polyamide hairpin (3) designed to target the inverted CCAAT box 2 (ICB2) site on the topoisomerase IIalpha (topoIIalpha) promoter are described. The polyamide component of 3 was derived from the minor-groove binder, 2, and tailored to bind to the 5'-TTGGT sequence found in and flanking ICB2. The propensity of mitonafide 4 to intercalate between G-C base pairs was exploited by the incorporation of a naphthalimide moiety at the N terminus of 2. Hybrid 3 targeted 5'-CGATTGGT and covered eight contiguous base pairs, which included the underlined ICB2 site. DNase I footprinting analysis with the topoIIalpha promoter sequence demonstrated that 3 bound selectively to the ICB2 and ICB3 sites. Thermal-denaturation studies confirmed these results, and the highest degree of stabilization was found for ICB2 and -3 in preference to ICB1 (4.1, 4.6, and 0.6 degrees C, respectively). CD studies confirmed minor-groove binding and suggested a 1:1 binding stoichiometry. Emission-titration experiments established intercalative binding. Surface plasmon resonance results showed strong binding to ICB2 (2.5x10(7) M(-1)) with no observable binding to ICB1. Furthermore, the binding constant of 3 to ICB2 was larger than that of the parent polyamide 2. The increased binding affinity was primarily due to a reduction in the dissociation-rate constant of the polyamide-DNA complex, which can be attributed to the N-terminal naphthalimide moiety. In addition, the binding site of 3 was larger than that of 2, which innately improved sequence selectivity. We conclude that the polyamide-naphthalimide 3 selectively binds to the ICB2 site by simultaneous intercalation and minor-groove binding, and warrants further investigation as a model compound for the regulation of topoIIalpha gene expression.