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131.
Buchanan HA Daéid NN Meier-Augenstein W Kemp HF Kerr WJ Middleditch M 《Analytical chemistry》2008,80(9):3350-3356
Drug profiling, or the ability to link batches of illicit drugs to a common source or synthetic route, has long been a goal of law enforcement agencies. Research in the past decade has explored drug profiling with isotope ratio mass spectrometry (IRMS). This type of research can be limited by the use of substances seized by police, of which the provenance is unknown. Fortunately, however, some studies in recent years have been carried out on drugs synthesized in-house and therefore of known history. In this study, 18 MDMA samples were synthesized in-house from aliquots of the same precursor by three common reductive amination routes and analyzed for 13C, 15N, and 2H isotope abundance using IRMS. For these three preparative methods, results indicate that 2H isotope abundance data is necessary for discrimination by synthetic route. Furthermore, hierarchical cluster analysis using 2H data on its own or combined with 13C and/or 15N provides a statistical means for accurate discrimination by synthetic route. 相似文献
132.
Ann?M.?McCauley Clain?A.?JonesEmail author Perry?R.?Miller Macdonald?H.?Burgess Catherine?A.?Zabinski 《Nutrient Cycling in Agroecosystems》2012,92(3):305-314
Crop-fallow systems dominate many semi-arid agricultural regions despite fallow’s negative effects on soil and water quality.
Annual legumes grown as a fallow-replacement crop, and terminated prior to maturity, can reduce these negative effects without
substantially decreasing plant available water for the subsequent crop. Interest in growing legume green manures (LGMs) in
synthetically-fertilized systems is increasing in the northern Great Plains of North America, partly due to the N-fixing capabilities
of legumes; however, little is known about the effects of planting and termination time on N fixation amounts in the region.
A 2-year field study was initiated in southwest Montana to determine the effects of planting time (spring or summer) and termination
time (e.g. flower or pod) on the amount of N fixed by field pea (Pisum sativum cv. Arvika) and lentil (Lens
culinaris cv. Richlea). Two methods, 15N natural abundance and N difference, were used to quantify N fixation, with wheat or in-crop weeds as reference plants. In
2009, N fixed by spring-planted lentil was higher by pod than flower (P = 0.03). Termination time did not affect the amount of N fixed by spring-planted pea, despite more biomass by pod than flower.
In 2010, both spring-planted crops fixed more N by pod than flower (P < 0.01) and more N was fixed by spring-planted than summer-planted crops (P < 0.01). These results should prove useful to growers interested in selecting management practices that optimize N fixation
of LGMs. 相似文献
133.
Hilary K Robie 《世界建筑导报》2012,(5):11-15
100年来,像底特律这样的城市已成为闻名世界的生产之都.美国人从未想过他们的工业丰碑会以我们正在见证的规模面临衰退、人口减少或者废弃.
今天,底特律给了我们机会修改我们对繁荣城市的看法.潜力在于工业城市向新兴都市形态的转型,该都市形态严重依赖农业生产以及副产品和服务网络以在城市中形成—个繁荣、多元的小经济体.最终,此类生产的目的不仅仅是自给自足,也是可持续的健康经济和居住环境的发展. 相似文献
134.
McDonnell MJ Rivas L Burgess CM Fanning S Duffy G 《International journal of food microbiology》2012,153(3):260-268
The antimic robial activities of caseicin A and B antimicrobial peptides (AMPs) were assessed against a selection of verocytotoxigenic Escherichia coli (VTEC) strains (n = 11), other bacterial pathogenic and spoilage bacteria (n = 7), using a model broth system. The ability of the AMPs to retain their antimicrobial activities against a strain of E. coli O157:H7 380-94 under various test conditions (pH, temperature, water activity, sodium chloride concentrations, inoculum size and the presence of competitive microflora) was assessed and the minimum inhibitory concentrations (MIC) and number of surviving E. coli O157:H7 calculated. The mean number of VTEC surviving after exposure to 2 mg/ml caseicin A and B was reduced by 4.96 and 4.19 log10 cfu/ml compared to the respective controls. The susceptibility of E. coli O157:H7 to the caseicin AMPs decreased as temperature, pH, water activity and inoculum size were reduced. The presence of sodium chloride (0.5-2.5%) did not affect the activity of caseicin A (p > 0.05), however it did inhibit the activity of caseicin B. The presence of a competitive microflora cocktail did not significantly (p > 0.05) affect the activities of the AMPs for the majority of the concentrations tested. Using a quantitative PCR assay, the levels of verotoxins (vt1 and vt2) expressed by E. coli O157:H7 following exposure to a sub-inhibitory concentration (0.5 mg/ml) of caseicin A showed that the verotoxin levels did not differ from the levels produced by the control cultures. The antimicrobial activity of caseicin A against E. coli O157:H7 was also tested in a model rumen system, however concentrations of ≥ 2 mg/ml did not significantly (p > 0.05) reduce E. coli O157:H7 numbers in the model system over a 24 h period. The application of caseicin AMPs in food and/or animal production may be valuable in combination with other antimicrobials although further research is required. 相似文献
135.
Hilary Schaffer Boudet Dilanka Chinthana Jayasundera Jennifer Davis 《Canadian Metallurgical Quarterly》2011,137(7):498-511
Despite the considerable scholarship focused on infrastructure investment in the developing world and the substantial sums of money spent each year on developing-country infrastructure, little attention has been given to understanding the drivers of conflict that shape the trajectory and cost structures of these massive investments. The manifestation of conflict among stakeholders in infrastructure projects ranges from the renegotiation of contract terms by project partners to popular protests among consumers of privatized services. The principal objective of this research is to identify combinations of country, project, and stakeholder factors that are associated with the emergence of legal and political conflict within natural gas and oil pipeline projects and water supply concessions and leases. The analysis includes data from 26 infrastructure projects spanning 31 countries and uses an analytical approach derived from Boolean algebra. Country-level characteristics, such as extent of democracy and rate of international NGO membership, are found to be important elements in the recipes for conflict among water supply projects but not for pipeline projects. Local impacts such as service price increases (water supply) and limited provision of oil and gas to the project host country (pipelines) are also important drivers of conflict for both subsectors. The involvement of one or more international financial institutions is also associated with the emergence of conflict in projects. Contrary to expectations, public consultation is associated with conflict in both subsectors. Overall, the study findings suggest that several factors associated with conflict in infrastructure projects can be minimized with careful project design. 相似文献
136.
Significant β-glucanolysis takes place during mashing and is catalysed by a β-glucanase which is specific to mixed-linkage β-glucans. The enzyme develops during the germination of barley, but is rapidly and extensively destroyed in kilning. Partially-purified preparations of β-glucanase are protected from denaturation by heat if their solutions are adjusted to pH 4 or if bovine serum albumin is added. However the most effective stabiliser of the enzyme is reduced glutathione. Oligosaccharides containing three and four glucosyl units are produced by the action of β-glucanase and they are further converted during malting and mashing by a different enzyme(s) to disaccharides and glucose. 相似文献
137.
Carbon and complex nitrogen source selection for secondary metabolite cultivation at the pilot scale
Junker B Mann Z Burgess B King J Greasham R 《Journal of Bioscience and Bioengineering》2001,91(5):462-468
The fermentation of desmethyl-asterriquinone B-1, a diabetes target, by a Pseudomonasarias species was conducted at the 600-l scale using a revised complex medium containing yeast extract and soy hydrolysate. Oat flour and tomato paste were removed from this medium due to difficulties in sterilization. An initial cerelose charge of 40 g/l improved titer and reduced product degradation in the broth at cultivation conditions. An initial mannitol concentration of 65 g/l effectively avoided mid-cycle mannitol additions necessary for the 40 g/l mannitol concentration without the reduction in productivity seen at 90 g/l mannitol. These additions diluted the broth because of the low aqueous solubility of mannitol. Titers reached 3.0 g/l after 158 h with an optimized process, increasing two-fold from the original medium and operating conditions. Reproducible foaming occurred at the point of glucose exhaustion when the culture switched to mannitol consumption. Use of alternative carbon sources (glycerol, soybean oil, sorbitol in conjunction with cerelose) was not effective in attaining similar productivity and did not reduce the extent of foaming. In the case of fructose, the extent of foaming was markedly reduced but product formation was negligible. 相似文献
138.
139.
Hilary Povey 《Pedagogy, Culture & Society》2000,8(2):219-231
The view that epistemological issues are central to developing a gender critical initial teacher education is explored. Particular reference is made to current government conceptualisations, and the associated assessment practices in England and Wales that are predicated on a technicist model of teaching. A critique of this model is put forward, and an alternative offered involving the self-reflective agent and a dialogic curriculum. Tentative suggestions are made for re-visioning practice 相似文献
140.
Ivask A Suarez E Patel T Boren D Ji Z Holden P Telesca D Damoiseaux R Bradley KA Godwin H 《Environmental science & technology》2012,46(4):2398-2405
By exploiting a genome-wide collection of bacterial single-gene deletion mutants, we have studied the toxicological pathways of a 60-nm cationic (amino-functionalized) polystyrene nanomaterial (PS-NH(2)) in bacterial cells. The IC(50) of commercially available 60 nm PS-NH(2) was determined to be 158 μg/mL, the IC(5) is 108 μg/mL, and the IC(90) is 190 μg/mL for the parent E. coli strain of the gene deletion library. Over 4000 single nonessential gene deletion mutants of Escherichia coli were screened for the growth phenotype of each strain in the presence and absence of PS-NH(2). This revealed that genes clusters in the lipopolysaccharide biosynthetic pathway, outer membrane transport channels, ubiquinone biosynthetic pathways, flagellar movement, and DNA repair systems are all important to how this organism responds to cationic nanomaterials. These results, coupled with those from confirmatory assays described herein, suggest that the primary mechanisms of toxicity of the 60-nm PS-NH(2) nanomaterial in E. coli are destabilization of the outer membrane and production of reactive oxygen species. The methodology reported herein should prove generally useful for identifying pathways that are involved in how cells respond to a broad range of nanomaterials and for determining the mechanisms of cellular toxicity of different types of nanomaterials. 相似文献